Rroxscaffold_2G00076200

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010061
Physical Location & Seq
Forward (+)
115570 .. 118006
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076200.1

Sequence Viewer

Length: 1215 bp
ATGGCTTCGGCTATTCTTTCTGTGGCCAAACCATCTCCTCAGGCAAATGGAAAGGGCTTTGCAGAATTCTCAGGTCTCCGCAACTCATCTGCAGCTTCGCTTTCTTTCGGGAGGAGAACCTCAGACGACTTCTTTTCAGTCATTGCCTTCCAAACCTCTGCTGCGGGAACTAACGGGGGATACAAAAGAGGGATAACGGAGGCGAAGCTGAAGGTGGCTATAAATGGGTTTGGAAGAATTGGAAGGAACTTCTTGAGGTGCTGGCATGGGCGCAAGGACTCACCTCTTGATGTGATTGCCATCAACGACACCGGAGGCGTGAAGCAGGCGTCTCACCTCCTCAAGTACGACTCTACCCTCGGGATCTTCGACGCTGACGTCAAGCCAGTTGGCGATGATGGCATCTCTGTTGACGGCAAGGTCATCAAGGTTGTGTCAAGCCGCAATCCCCTCAACCTACCTTGGAAGGACTTGGGGATCGACCTGGTGATTGAAGGCACTGGAGTGTTTGTTGATAGGGAGGGTGCCGGGAAACACATAGAGGCAGGGGCCAAGAAGGTGCTCATCACTGCCCCCGGAAAGGGCGACATCCCTACCTATGTGGTCGGGGTCAATGCTGAACTCTACAGTCATGATGATACCATCATTAGCAATGCTTCTTGCACCACCAACTGCCTTGCCCCCTTCGTCAAGGTTCTTGACCAGAAGTTTGGCATCATCAAGGGCACCATGACTACCACACACTCCTACACTGGTGACCAAAGGCTACTTGATGCCAGCCACCGTGACCTCAGGCGTGCACGAGCTGCTGCTCTCAACATAGTTCCCACTTCAACTGGTGCAGCAAAGGCCGTTGCTCTAGTCCTTCCATCTCTCAAAGGAAAGCTAAATGGCATTGCCCTGCGTGTGCCCACCCCCAATGTCTCCGTCGTCGACCTTGTTGTCCAGGTCACTAAGAAGACCTTTGCAGAGGAGGTTAATGCTGCTTTCAGAGAGAGTGCTGATAAGGAGCTGGCAGGTATCCTTTCTGTTTGCGATGAGCCCCTTGTTTCTGTCGATTTCAGGTGCACAGATGTATCGTCAACAGTTGACTCCTCATTGACTATGGTGATGGGAGATGACATGGTCAAGGTCATTGCTTGGTACGATAACGAATGGGGTTACTCACAAAGGGTTGTTGACTTGGCTGATATTGTCGCCAACAACTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00710 ko01100 ko01120 ko01200 map00710 map01100 map01120 map01200
Pfam Domains
Protein Families
Gp_dh_C Gp_dh_N

Protein Analysis

404

Amino Acids

43.06

Weight (kDa)

8.16

Isoelectric Point (pI)

24.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gp_dh_N PF00044 71 - 172 3.3e-31 Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
Gp_dh_C PF02800 226 - 382 4.5e-66 Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010061)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12900 AT1G12900 AT1G12900 AT1G12900 AT3G26650
fragaria_vesca FvH4_6g54460
malus_domestica MD09G1000800.v1.1 MD17G1003500.v1.1
prunus_persica Prupe.3G315500_v2.0.a1
pyrus_communis pycom111g00050 pycom17g00030
rosa_chinensis RchiOBHm_Chr2g0176741
rosa_laevigata RLG00000022441
rosa_multiflora Rmu_ssc0000468.1_g000003
rosa_roxburghii Rroxscaffold_2G00076200
rosa_rugosa Rorug02G0598200
rosa_samantha Rh2AG675500 Rh2BG686800 Rh2CG650100 Rh2DG701000
rosa_wichuraiana Rw2G055440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 377, 419, 941
AatII GACGTC 1 cut(s) 381
Acc36I ACCTGC 1 cut(s) 1007
AccB1I GGYRCC 2 cut(s) 524, 725
AccB7I CCANNNNNTGG 1 cut(s) 1206
AccI GTMKAC 1 cut(s) 933
AciI CCGC 3 cut(s) 79, 164, 442
AclWI GGATC 2 cut(s) 371, 485
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 2 cut(s) 329, 378
AfaI GTAC 2 cut(s) 347, 1145
AfiI CCNNNNNNNGG 3 cut(s) 580, 581, 1206
AgsI TTSAA 2 cut(s) 494, 834
AjnI CCWGG 2 cut(s) 483, 945
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 5 cut(s) 95, 208, 806, 886, 1012
AluI AGCT 5 cut(s) 95, 208, 806, 886, 1012
Alw21I GWGCWC 3 cut(s) 564, 802, 1070
Alw26I GTCTC 3 cut(s) 80, 336, 928
Alw44I GTGCAC 2 cut(s) 798, 1066
AlwI GGATC 2 cut(s) 371, 485
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 3 cut(s) 24, 549, 849
ApaLI GTGCAC 2 cut(s) 798, 1066
ApeKI GCWGC 6 cut(s) 92, 161, 806, 809, 842, 983
ApoI RAATTY 1 cut(s) 65
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 549
AsuC2I CCSGG 2 cut(s) 529, 576
AsuHPI GGTGA 5 cut(s) 273, 326, 499, 767, 1120
AvaI CYCGRG 1 cut(s) 359
AxyI CCTNAGG 2 cut(s) 39, 791
BaeGI GKGCMC 4 cut(s) 728, 802, 912, 1070
BaeI ACNNNNGTAYC 2 cut(s) 1059, 1092
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 2 cut(s) 524, 725
BanII GRGCYC 1 cut(s) 1044
BauI CACGAG 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 965
Bbv12I GWGCWC 3 cut(s) 564, 802, 1070
BbvI GCAGC 6 cut(s) 104, 148, 793, 796, 854, 970
BccI CCATC 6 cut(s) 40, 308, 392, 650, 877, 1105
BceAI ACGGC 2 cut(s) 430, 836
BciT130I CCWGG 2 cut(s) 485, 947
BciVI GTATCC 2 cut(s) 173, 1031
BcnI CCSGG 2 cut(s) 529, 576
BcoDI GTCTC 3 cut(s) 80, 336, 928
BfaI CTAG 1 cut(s) 860
BfmI CTRYAG 2 cut(s) 90, 625
BfuAI ACCTGC 1 cut(s) 1007
BfuI GTATCC 2 cut(s) 173, 1031
BisI GCNGC 7 cut(s) 93, 162, 442, 807, 810, 843, 984
BlsI GCNGC 7 cut(s) 94, 163, 443, 808, 811, 844, 985
Bme1390I CCNGG 4 cut(s) 485, 529, 576, 947
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 549
BmiI GGNNCC 3 cut(s) 526, 550, 727
BmrFI CCNGG 4 cut(s) 485, 529, 576, 947
BmsI GCATC 3 cut(s) 411, 723, 763
BpiI GAAGAC 1 cut(s) 965
BpmI CTGGAG 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 274, 326
BpuMI CCSGG 2 cut(s) 529, 576
BsaBI GATNNNNATC 1 cut(s) 299
BsaHI GRCGYC 2 cut(s) 329, 378
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 358, 461, 574
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 3 cut(s) 580, 581, 1206
Bse1I ACTGG 5 cut(s) 386, 505, 757, 841, 1211
Bse21I CCTNAGG 2 cut(s) 39, 791
Bse3DI GCAATG 4 cut(s) 141, 658, 894, 1134
Bse8I GATNNNNATC 1 cut(s) 299
BseBI CCWGG 2 cut(s) 485, 947
BseDI CCNNGG 3 cut(s) 358, 461, 574
BseGI GGATG 1 cut(s) 588
BseJI GATNNNNATC 1 cut(s) 299
BseLI CCNNNNNNNGG 3 cut(s) 580, 581, 1206
BseMI GCAATG 4 cut(s) 141, 658, 894, 1134
BseMII CTCAG 4 cut(s) 53, 84, 135, 805
BseNI ACTGG 5 cut(s) 386, 505, 757, 841, 1211
BseRI GAGGAG 5 cut(s) 27, 127, 329, 986, 1084
BseSI GKGCMC 4 cut(s) 728, 802, 912, 1070
BseXI GCAGC 6 cut(s) 104, 148, 793, 796, 854, 970
BsgI GTGCAG 1 cut(s) 861
BshFI GGCC 3 cut(s) 26, 551, 851
BshNI GGYRCC 2 cut(s) 524, 725
BsiHKAI GWGCWC 3 cut(s) 564, 802, 1070
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 3 cut(s) 312, 528, 576
BslI CCNNNNNNNGG 3 cut(s) 580, 581, 1206
BsmAI GTCTC 3 cut(s) 80, 336, 928
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 3 cut(s) 26, 551, 851
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 6 cut(s) 564, 728, 802, 912, 1044, 1070
Bsp143I GATC 2 cut(s) 363, 477
BspACI CCGC 3 cut(s) 79, 164, 442
BspANI GGCC 3 cut(s) 26, 551, 851
BspCNI CTCAG 4 cut(s) 52, 83, 134, 804
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 3 cut(s) 526, 550, 727
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 1007
BspPI GGATC 2 cut(s) 371, 485
BspT107I GGYRCC 2 cut(s) 524, 725
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 4 cut(s) 141, 658, 894, 1134
BsrI ACTGG 5 cut(s) 386, 505, 757, 841, 1211
BssECI CCNNGG 3 cut(s) 358, 461, 574
BssMI GATC 2 cut(s) 363, 477
BssNI GRCGYC 2 cut(s) 329, 378
BssSI CACGAG 1 cut(s) 801
BssT1I CCWWGG 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 801
Bst2UI CCWGG 2 cut(s) 485, 947
Bst4CI ACNGT 3 cut(s) 629, 785, 1087
BstACI GRCGYC 2 cut(s) 329, 378
BstAPI GCANNNNNTGC 1 cut(s) 806
BstC8I GCNNGC 5 cut(s) 263, 327, 778, 798, 1014
BstDEI CTNAG 5 cut(s) 39, 70, 121, 791, 954
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 1 cut(s) 588
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 2 cut(s) 366, 480
BstMAI GTCTC 3 cut(s) 80, 336, 928
BstMBI GATC 2 cut(s) 363, 477
BstMWI GCNNNNNNNGC 3 cut(s) 399, 806, 848
BstNI CCWGG 2 cut(s) 485, 947
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 4 cut(s) 483, 527, 574, 945
BstSFI CTRYAG 2 cut(s) 90, 625
BstSLI GKGCMC 4 cut(s) 728, 802, 912, 1070
BstV1I GCAGC 6 cut(s) 104, 148, 793, 796, 854, 970
BstV2I GAAGAC 1 cut(s) 965
BstX2I RGATCY 1 cut(s) 363
BstXI CCANNNNNNTGG 1 cut(s) 710
BstYI RGATCY 1 cut(s) 363
Bsu36I CCTNAGG 2 cut(s) 39, 791
BsuI GTATCC 2 cut(s) 173, 1031
BsuRI GGCC 3 cut(s) 26, 551, 851
BtgZI GCGATG 2 cut(s) 408, 1050
BtsCI GGATG 1 cut(s) 588
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 3 cut(s) 498, 567, 750
BveI ACCTGC 1 cut(s) 1007
Cac8I GCNNGC 5 cut(s) 263, 327, 778, 798, 1014
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 549
CseI GACGC 2 cut(s) 318, 380
CsiI ACCWGGT 1 cut(s) 483
Csp6I GTAC 2 cut(s) 346, 1144
CviAII CATG 4 cut(s) 266, 632, 730, 1123
CviQI GTAC 2 cut(s) 346, 1144
DdeI CTNAG 5 cut(s) 39, 70, 121, 791, 954
DpnI GATC 2 cut(s) 365, 479
DpnII GATC 2 cut(s) 363, 477
DrdI GACNNNNNNGTC 3 cut(s) 377, 419, 941
DseDI GACNNNNNNGTC 3 cut(s) 377, 419, 941
EaeI YGGCCR 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 1044
Eco31I GGTCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 230
Eco81I CCTNAGG 2 cut(s) 39, 791
Eco88I CYCGRG 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 755
EcoO65I GGTNACC 1 cut(s) 755
EcoRI GAATTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 483, 945
EcoT14I CCWWGG 1 cut(s) 461
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 4 cut(s) 269, 635, 733, 1126
FaiI YATR 9 cut(s) 221, 267, 539, 600, 633, 731, 821, 1106, 1124
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FatI CATG 4 cut(s) 265, 631, 729, 1122
FauI CCCGC 1 cut(s) 157
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 7 cut(s) 93, 162, 442, 807, 810, 843, 984
FokI GGATG 1 cut(s) 575
FriOI GRGCYC 1 cut(s) 1044
Fsp4HI GCNGC 7 cut(s) 93, 162, 442, 807, 810, 843, 984
FspBI CTAG 1 cut(s) 860
GlaI GCGC 1 cut(s) 272
GluI GCNGC 7 cut(s) 93, 162, 442, 807, 810, 843, 984
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 3 cut(s) 26, 551, 851
HapII CCGG 3 cut(s) 312, 528, 576
HgaI GACGC 2 cut(s) 318, 380
HhaI GCGC 1 cut(s) 273
Hin1I GRCGYC 2 cut(s) 329, 378
Hin1II CATG 4 cut(s) 269, 635, 733, 1126
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HincII GTYRAC 5 cut(s) 412, 934, 1083, 1090, 1180
HindII GTYRAC 5 cut(s) 412, 934, 1083, 1090, 1180
HinfI GANTC 3 cut(s) 278, 350, 1091
HpaII CCGG 3 cut(s) 312, 528, 576
HphI GGTGA 5 cut(s) 273, 326, 499, 767, 1120
Hpy166II GTNNAC 7 cut(s) 412, 800, 934, 1068, 1083, 1090, 1180
Hpy188I TCNGA 2 cut(s) 124, 992
Hpy188III TCNNGA 6 cut(s) 109, 253, 287, 361, 632, 698
Hpy8I GTNNAC 7 cut(s) 412, 800, 934, 1068, 1083, 1090, 1180
Hpy99I CGWCG 3 cut(s) 374, 932, 935
HpyAV CCTTC 8 cut(s) 157, 205, 237, 460, 488, 550, 694, 875
HpyCH4III ACNGT 3 cut(s) 629, 785, 1087
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 7 cut(s) 62, 92, 663, 800, 842, 968, 1068
HpyF10VI GCNNNNNNNGC 3 cut(s) 399, 806, 848
HpyF3I CTNAG 5 cut(s) 39, 70, 121, 791, 954
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 2 cut(s) 329, 378
Hsp92II CATG 4 cut(s) 269, 635, 733, 1126
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 2 cut(s) 363, 477
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 6 cut(s) 104, 148, 793, 796, 854, 970
LweI GCATC 3 cut(s) 411, 723, 763
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 1 cut(s) 860
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 4 cut(s) 755, 785, 949, 1160
MalI GATC 2 cut(s) 365, 479
MboI GATC 2 cut(s) 363, 477
MboII GAAGA 3 cut(s) 246, 358, 970
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 6 cut(s) 564, 728, 802, 912, 1044, 1070
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 2 cut(s) 65, 237
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 3 cut(s) 272, 344, 1085
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
MseI TTAA 1 cut(s) 978
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 26
MspI CCGG 3 cut(s) 312, 528, 576
MspR9I CCNGG 4 cut(s) 485, 529, 576, 947
MvaI CCWGG 2 cut(s) 485, 947
MwoI GCNNNNNNNGC 3 cut(s) 399, 806, 848
NciI CCSGG 2 cut(s) 529, 576
NdeII GATC 2 cut(s) 363, 477
NlaIII CATG 4 cut(s) 269, 635, 733, 1126
NlaIV GGNNCC 3 cut(s) 526, 550, 727
NmuCI GTSAC 3 cut(s) 755, 785, 949
OliI CACNNNNGTG 1 cut(s) 503
PagI TCATGA 1 cut(s) 631
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 375
PflFI GACNNNGTC 1 cut(s) 1124
PflMI CCANNNNNTGG 1 cut(s) 1206
PkrI GCNGC 7 cut(s) 94, 163, 443, 808, 811, 844, 985
PleI GAGTC 3 cut(s) 272, 344, 1085
PpsI GAGTC 3 cut(s) 272, 344, 1085
Psp6I CCWGG 2 cut(s) 483, 945
PspEI GGTNACC 1 cut(s) 755
PspGI CCWGG 2 cut(s) 483, 945
PspN4I GGNNCC 3 cut(s) 526, 550, 727
PspPI GGNCC 1 cut(s) 549
PstI CTGCAG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 363
PsyI GACNNNGTC 1 cut(s) 1124
RsaI GTAC 2 cut(s) 347, 1145
RsaNI GTAC 2 cut(s) 346, 1144
RseI CAYNNNNRTG 1 cut(s) 503
SalI GTCGAC 1 cut(s) 932
SaqAI TTAA 1 cut(s) 978
SatI GCNGC 7 cut(s) 93, 162, 442, 807, 810, 843, 984
Sau3AI GATC 2 cut(s) 363, 477
Sau96I GGNCC 1 cut(s) 549
SchI GAGTC 3 cut(s) 272, 344, 1085
ScrFI CCNGG 4 cut(s) 485, 529, 576, 947
SduI GDGCHC 6 cut(s) 564, 728, 802, 912, 1044, 1070
SexAI ACCWGGT 1 cut(s) 483
SfaNI GCATC 3 cut(s) 411, 723, 763
SfcI CTRYAG 2 cut(s) 90, 625
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 2 cut(s) 253, 341
SmoI CTYRAG 2 cut(s) 253, 341
Sse9I AATT 2 cut(s) 65, 237
SsiI CCGC 3 cut(s) 79, 164, 442
SspMI CTAG 1 cut(s) 860
StyD4I CCNGG 4 cut(s) 483, 527, 574, 945
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 3 cut(s) 629, 785, 1087
TaiI ACGT 1 cut(s) 381
TaqI TCGA 4 cut(s) 369, 480, 933, 1056
TasI AATT 2 cut(s) 65, 237
TauI GCSGC 1 cut(s) 444
Tru1I TTAA 1 cut(s) 978
Tru9I TTAA 1 cut(s) 978
TscAI CASTG 3 cut(s) 505, 574, 757
TseFI GTSAC 3 cut(s) 755, 785, 949
TseI GCWGC 6 cut(s) 92, 161, 806, 809, 842, 983
Tsp45I GTSAC 3 cut(s) 755, 785, 949
TspGWI ACGGA 2 cut(s) 212, 916
TspRI CASTG 3 cut(s) 505, 574, 757
Tth111I GACNNNGTC 1 cut(s) 1124
Van91I CCANNNNNTGG 1 cut(s) 1206
VneI GTGCAC 2 cut(s) 798, 1066
XapI RAATTY 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 933
XspI CTAG 1 cut(s) 860
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.