MD17G1022100.v1.1

TruB family pseudouridylate synthase (N terminal domain)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014138
Physical Location & Seq
Reverse (-)
1639624 .. 1642980
3357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1022100.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGCTAAGCCAGTCTCTCTGTCTCTCCTCTTCCTCCGTCCCACACTCACACTCTCTCGCTTACTGCGACCGCACCCCAACCTCCGCACCGTACTCTGCCGATTATGGTCCCCCAAAACGCCGTCGTTCTCAACCACCTCCACTCCCTACCCACTTCAGTACGACATGATCGTCAACCGCCCAACTCAGTCCTCCCTCGACCACAATCGCCGCCGACCCGCCAGAGTCAGCAGACCGGACTCCGAAACCTCCCTCGAGTCCGAACCCGACTTACCGGAAAAACCCACCTCGGCGTCGGAATTGGGGTTCGAGAACTGGCTCGACGAGAAGCTGGCGTCGGCGGAGATGGACAAGTCGAAGCGAAAGTACTACAATAAGAGGAGGAAGAGGATGTACGGGACGGATTCGGAGGAGGAGGACCGGCGGCGGGAGGAGGAGGAGAGCTTGGTGGAGCTCAAGCCGGAGGTGGTTGAGTTCAACACGCTGCATAAGAGGGAGGAGGAATTGTACTTTTACGACACGTTTGCGTATCCATGGGAGAAGGAGAAGCATTACAAGATGGTGTACCAGCTGGAGAAGAAGTACTATCCTGATCAGTGTCTCGAAAAGGCGTTTCTTGAACCAGGGGAGTCAAATTCCGGCGTCAATAGCGATGTCAATTTGAAGGGGAAGGCAAAGCCGAAGAAAACGAAAGACGGGGAAGGTGATAAGGGATTGGTGTTCTTTGAGCAGGCGGAGAGAGAGGAGAAGAAGGAGAGAGAATTCGTGAAGAATGTGAGTAGTAAAGATGTGACAGAGAAGAAGGTGGAGGACTTCTTTAAGTGTTTGAAGAAAGTCCCCAATGAAGATGCTGGCAGTGGCGATGGTAATGGGGAGCCATATCTTTTAACAAGGAATACTGAGCTTCCTCCGAGATGGGACGGTCCTTATGGAACTGTGGTTTTGGTGAACAAACCGAAAGGATGGACTTCTTTTACAGTTTGCGGAAAGCTGCGTCGCCTGGTGAAAGTGAAAAAGGTAGGGCATGCTGGAACTCTTGATCCTATGGCAACTGGTTTGTTAATTGTTTGTGTTGGTAAAGCCACTAAGTTGGTAGACGGATACCAAGGTATGATCAAGGGGTATAGTGGAGTTTTCCGTTTGGGGGAGGCTACTTCAACTTGGGATGCAGATTCACCGGTCATTCAGCGCGAACCCTGGGAGCATATCAAAGATGAAGACATAAAGAAAGCTGTTGCATCCTTTTCTGGGGAGATCTGGCAAGTACCTCCCATGTTTTCTGCCATCAAAGTTGGAGGTGAAAAGATGTATGAGAAAGCAAGAAGAGGAGAAAGCATTGAGCTTTCACCTAGACGTATATCAATCTTCCAGTTTGACATTGAGCGCAGCTTAGATGACAGACAAAATTTGATCTTCCGGGTTATTTGCTCCAAAGGGACATATATTCGGTCGCTATGTGCGGACTTGGGGAAGGCTCTTGGAAGCTGTGCCCATTTAACTGCTCTTCGAAGAGATTCAATCGGGGAATACTCTGCAGACAATGCCTGGGACTTCAAAGAGCTGGAAGAGGCAATTACCAAAACTTATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TruB_C_2 TruB_N

Protein Analysis

531

Amino Acids

60.75

Weight (kDa)

8.49

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TruB_N PF01509 332 - 482 5.8e-52 TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2 PF16198 483 - 526 5.9e-09 tRNA pseudouridylate synthase B C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14460
fragaria_vesca FvH4_6g51780 FvH4_6g51780
malus_domestica MD17G1022100.v1.1
prunus_persica Prupe.3G294000_v2.0.a1
pyrus_communis pycom17g01920
rosa_chinensis RchiOBHm_Chr2g0173311
rosa_laevigata RLG00000022178
rosa_multiflora Rmu_sc0010961.1_g000005
rosa_roxburghii Rroxscaffold_2G00079060
rosa_rugosa Rorug02G0567200 Rorug02G0567300
rosa_samantha Rh2AG647600 Rh2BG658100 Rh2CG623500 Rh2DG671900
rosa_wichuraiana Rw2G053160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AccI GTMKAC 1 cut(s) 1095
AccII CGCG 1 cut(s) 1191
AclWI GGATC 1 cut(s) 1034
AcsI RAATTY 3 cut(s) 634, 761, 1405
AcuI CTGAAG 1 cut(s) 140
AcyI GRCGYC 3 cut(s) 293, 335, 642
AfaI GTAC 8 cut(s) 93, 161, 368, 395, 509, 566, 584, 1266
AfiI CCNNNNNNNGG 3 cut(s) 427, 1144, 1248
AflIII ACRYGT 1 cut(s) 519
AgeI ACCGGT 1 cut(s) 1177
AgsI TTSAA 7 cut(s) 478, 620, 664, 829, 1158, 1518, 1555
AjnI CCWGG 4 cut(s) 622, 999, 1196, 1544
AjuI GAANNNNNNNTTGG 1 cut(s) 1571
AloI GAACNNNNNNTCC 4 cut(s) 290, 322, 1024, 1056
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 3 cut(s) 19, 27, 605
AlwI GGATC 1 cut(s) 1034
Ama87I CYCGRG 1 cut(s) 254
ApeKI GCWGC 3 cut(s) 484, 991, 1386
ApoI RAATTY 3 cut(s) 634, 761, 1405
AsiGI ACCGGT 1 cut(s) 1177
Asp700I GAANNNNTTC 1 cut(s) 1513
AspLEI GCGC 2 cut(s) 1191, 1386
AspS9I GGNCC 3 cut(s) 108, 418, 923
AsuC2I CCSGG 1 cut(s) 1418
AsuHPI GGTGA 6 cut(s) 716, 958, 1015, 1167, 1310, 1338
AsuII TTCGAA 1 cut(s) 1507
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 3 cut(s) 108, 418, 923
BaeGI GKGCMC 1 cut(s) 1492
BaeI ACNNNNGTAYC 2 cut(s) 1093, 1126
BanII GRGCYC 1 cut(s) 456
BarI GAAGNNNNNNTAC 4 cut(s) 350, 377, 382, 409
BbsI GAAGAC 1 cut(s) 1224
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 3 cut(s) 471, 978, 1398
BccI CCATC 6 cut(s) 340, 553, 857, 909, 957, 1292
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 4 cut(s) 624, 1001, 1198, 1546
BciVI GTATCC 2 cut(s) 540, 1094
BclI TGATCA 2 cut(s) 592, 1113
BcnI CCSGG 1 cut(s) 1418
BcoDI GTCTC 3 cut(s) 19, 27, 605
BfaI CTAG 1 cut(s) 1350
BfmI CTRYAG 1 cut(s) 1533
BfuI GTATCC 2 cut(s) 540, 1094
BglII AGATCT 1 cut(s) 1254
BisI GCNGC 5 cut(s) 211, 425, 485, 992, 1387
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 5 cut(s) 212, 426, 486, 993, 1388
BmcAI AGTACT 2 cut(s) 368, 584
Bme1390I CCNGG 5 cut(s) 624, 1001, 1198, 1418, 1546
Bme18I GGWCC 3 cut(s) 108, 418, 923
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 3 cut(s) 108, 418, 923
BmiI GGNNCC 2 cut(s) 110, 876
BmrFI CCNGG 5 cut(s) 624, 1001, 1198, 1418, 1546
BmsI GCATC 3 cut(s) 838, 1156, 1247
BpiI GAAGAC 1 cut(s) 1224
BpmI CTGGAG 1 cut(s) 593
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 1507
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 1418
BsaHI GRCGYC 3 cut(s) 293, 335, 642
BsaJI CCNNGG 7 cut(s) 288, 533, 623, 1105, 1196, 1197, 1545
BsaWI WCCGGW 3 cut(s) 235, 274, 1177
BsaXI ACNNNNNCTCC 6 cut(s) 127, 157, 491, 521, 566, 596
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 1144, 1248
Bse118I RCCGGY 2 cut(s) 420, 1177
Bse1I ACTGG 4 cut(s) 11, 320, 1057, 1369
BseBI CCWGG 4 cut(s) 624, 1001, 1198, 1546
BseDI CCNNGG 7 cut(s) 288, 533, 623, 1105, 1196, 1197, 1545
BseGI GGATG 4 cut(s) 396, 968, 1171, 1238
BseLI CCNNNNNNNGG 3 cut(s) 427, 1144, 1248
BseMII CTCAG 2 cut(s) 200, 891
BseNI ACTGG 4 cut(s) 11, 320, 1057, 1369
BseSI GKGCMC 1 cut(s) 1492
BseXI GCAGC 3 cut(s) 471, 978, 1398
Bsh1236I CGCG 1 cut(s) 1191
Bsh1285I CGRYCG 2 cut(s) 71, 1451
BshTI ACCGGT 1 cut(s) 1177
BsiEI CGRYCG 2 cut(s) 71, 1451
BsiHKAI GWGCWC 1 cut(s) 456
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 7 cut(s) 236, 275, 421, 461, 639, 1178, 1417
BslFI GGGAC 7 cut(s) 24, 94, 412, 821, 932, 1450, 1562
BslI CCNNNNNNNGG 3 cut(s) 427, 1144, 1248
BsmAI GTCTC 3 cut(s) 19, 27, 605
BsmFI GGGAC 7 cut(s) 24, 94, 412, 821, 932, 1450, 1562
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 1507
Bsp1286I GDGCHC 2 cut(s) 456, 1492
Bsp143I GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 199, 892
BspFNI CGCG 1 cut(s) 1191
BspLI GGNNCC 2 cut(s) 110, 876
BspMAI CTGCAG 1 cut(s) 1537
BspPI GGATC 1 cut(s) 1034
BspQI GCTCTTC 1 cut(s) 1509
BspT104I TTCGAA 1 cut(s) 1507
BsrFI RCCGGY 2 cut(s) 420, 1177
BsrI ACTGG 4 cut(s) 11, 320, 1057, 1369
BssAI RCCGGY 2 cut(s) 420, 1177
BssECI CCNNGG 7 cut(s) 288, 533, 623, 1105, 1196, 1197, 1545
BssMI GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
BssNI GRCGYC 3 cut(s) 293, 335, 642
BssT1I CCWWGG 2 cut(s) 533, 1105
Bst2UI CCWGG 4 cut(s) 624, 1001, 1198, 1546
Bst4CI ACNGT 4 cut(s) 91, 923, 937, 979
Bst6I CTCTTC 6 cut(s) 35, 380, 1318, 1504, 1509, 1560
BstACI GRCGYC 3 cut(s) 293, 335, 642
BstAPI GCANNNNNTGC 1 cut(s) 1541
BstBI TTCGAA 1 cut(s) 1507
BstC8I GCNNGC 4 cut(s) 333, 732, 853, 1026
BstDEI CTNAG 5 cut(s) 6, 186, 900, 1086, 1390
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 4 cut(s) 396, 968, 1171, 1238
BstFNI CGCG 1 cut(s) 1191
BstHHI GCGC 2 cut(s) 1191, 1386
BstKTI GATC 6 cut(s) 171, 595, 1042, 1116, 1257, 1413
BstMAI GTCTC 3 cut(s) 19, 27, 605
BstMBI GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
BstMCI CGRYCG 2 cut(s) 71, 1451
BstMWI GCNNNNNNNGC 2 cut(s) 648, 1541
BstNI CCWGG 4 cut(s) 624, 1001, 1198, 1546
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 5 cut(s) 622, 999, 1196, 1416, 1544
BstSFI CTRYAG 1 cut(s) 1533
BstSLI GKGCMC 1 cut(s) 1492
BstUI CGCG 1 cut(s) 1191
BstV1I GCAGC 3 cut(s) 471, 978, 1398
BstV2I GAAGAC 1 cut(s) 1224
BstX2I RGATCY 1 cut(s) 1254
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 1254
BsuI GTATCC 2 cut(s) 540, 1094
BtgI CCRYGG 1 cut(s) 533
BtgZI GCGATG 2 cut(s) 666, 876
BtsCI GGATG 4 cut(s) 396, 968, 1171, 1238
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 2 cut(s) 602, 862
Cac8I GCNNGC 4 cut(s) 333, 732, 853, 1026
CfoI GCGC 2 cut(s) 1191, 1386
Cfr10I RCCGGY 2 cut(s) 420, 1177
Cfr13I GGNCC 3 cut(s) 108, 418, 923
CseI GACGC 4 cut(s) 282, 324, 631, 983
Csp6I GTAC 8 cut(s) 92, 160, 367, 394, 508, 565, 583, 1265
CspAI ACCGGT 1 cut(s) 1177
CviAII CATG 4 cut(s) 166, 534, 1025, 1273
CviQI GTAC 8 cut(s) 92, 160, 367, 394, 508, 565, 583, 1265
DdeI CTNAG 5 cut(s) 6, 186, 900, 1086, 1390
DpnI GATC 6 cut(s) 170, 594, 1041, 1115, 1256, 1412
DpnII GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 6 cut(s) 35, 380, 1318, 1504, 1509, 1560
EarI CTCTTC 6 cut(s) 35, 380, 1318, 1504, 1509, 1560
EciI GGCGGA 2 cut(s) 356, 749
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 2 cut(s) 533, 1105
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 3 cut(s) 108, 418, 923
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 4 cut(s) 622, 999, 1196, 1544
EcoT14I CCWWGG 2 cut(s) 533, 1105
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 2 cut(s) 533, 1105
FaeI CATG 4 cut(s) 169, 537, 1028, 1276
FaqI GGGAC 7 cut(s) 24, 94, 412, 821, 932, 1450, 1562
FatI CATG 4 cut(s) 165, 533, 1024, 1272
FauI CCCGC 2 cut(s) 226, 420
FbaI TGATCA 2 cut(s) 592, 1113
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 5 cut(s) 211, 425, 485, 992, 1387
FokI GGATG 4 cut(s) 403, 975, 1178, 1225
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 5 cut(s) 211, 425, 485, 992, 1387
FspBI CTAG 1 cut(s) 1350
GlaI GCGC 2 cut(s) 1190, 1385
GluI GCNGC 5 cut(s) 211, 425, 485, 992, 1387
GsuI CTGGAG 1 cut(s) 593
HapII CCGG 7 cut(s) 236, 275, 421, 461, 639, 1178, 1417
HgaI GACGC 4 cut(s) 282, 324, 631, 983
HhaI GCGC 2 cut(s) 1191, 1386
Hin1I GRCGYC 3 cut(s) 293, 335, 642
Hin1II CATG 4 cut(s) 169, 537, 1028, 1276
Hin6I GCGC 2 cut(s) 1189, 1384
HinP1I GCGC 2 cut(s) 1189, 1384
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 7 cut(s) 225, 239, 257, 404, 629, 1172, 1514
HpaII CCGG 7 cut(s) 236, 275, 421, 461, 639, 1178, 1417
HphI GGTGA 6 cut(s) 716, 958, 1015, 1167, 1310, 1338
Hpy166II GTNNAC 4 cut(s) 175, 565, 949, 1096
Hpy188I TCNGA 5 cut(s) 244, 262, 298, 409, 912
Hpy188III TCNNGA 6 cut(s) 310, 590, 602, 617, 766, 1037
Hpy8I GTNNAC 4 cut(s) 175, 565, 949, 1096
Hpy99I CGWCG 5 cut(s) 127, 298, 326, 340, 999
HpyAV CCTTC 7 cut(s) 535, 658, 664, 695, 745, 796, 1465
HpyCH4III ACNGT 4 cut(s) 91, 923, 937, 979
HpyCH4IV ACGT 2 cut(s) 521, 1354
HpyCH4V TGCA 4 cut(s) 487, 1169, 1238, 1535
HpyF10VI GCNNNNNNNGC 2 cut(s) 648, 1541
HpyF3I CTNAG 5 cut(s) 6, 186, 900, 1086, 1390
HpySE526I ACGT 2 cut(s) 521, 1354
Hsp92I GRCGYC 3 cut(s) 293, 335, 642
Hsp92II CATG 4 cut(s) 169, 537, 1028, 1276
HspAI GCGC 2 cut(s) 1189, 1384
Ksp22I TGATCA 2 cut(s) 592, 1113
Kzo9I GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
LguI GCTCTTC 1 cut(s) 1509
LmnI GCTCC 4 cut(s) 451, 874, 1201, 1433
Lsp1109I GCAGC 3 cut(s) 471, 978, 1398
LweI GCATC 3 cut(s) 838, 1156, 1247
MaeI CTAG 1 cut(s) 1350
MaeII ACGT 2 cut(s) 521, 1354
MaeIII GTNAC 1 cut(s) 790
MalI GATC 6 cut(s) 170, 594, 1041, 1115, 1256, 1412
MboI GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
MflI RGATCY 1 cut(s) 1254
MhlI GDGCHC 2 cut(s) 456, 1492
MluCI AATT 8 cut(s) 299, 503, 634, 658, 761, 1062, 1405, 1572
MlyI GAGTC 4 cut(s) 233, 234, 266, 638
MmeI TCCRAC 2 cut(s) 276, 1273
MroXI GAANNNNTTC 1 cut(s) 1513
MseI TTAA 4 cut(s) 819, 887, 1061, 1496
MspA1I CMGCKG 1 cut(s) 571
MspI CCGG 7 cut(s) 236, 275, 421, 461, 639, 1178, 1417
MspR9I CCNGG 5 cut(s) 624, 1001, 1198, 1418, 1546
MvaI CCWGG 4 cut(s) 624, 1001, 1198, 1546
MvnI CGCG 1 cut(s) 1191
MwoI GCNNNNNNNGC 2 cut(s) 648, 1541
NciI CCSGG 1 cut(s) 1418
NcoI CCATGG 1 cut(s) 533
NdeII GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
NlaIII CATG 4 cut(s) 169, 537, 1028, 1276
NlaIV GGNNCC 2 cut(s) 110, 876
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 790
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 1507
PaeI GCATGC 1 cut(s) 1028
PaeR7I CTCGAG 1 cut(s) 254
PasI CCCWGGG 1 cut(s) 1197
PciSI GCTCTTC 1 cut(s) 1509
PcsI WCGNNNNNNNCGW 2 cut(s) 64, 168
PdmI GAANNNNTTC 1 cut(s) 1513
PfeI GAWTC 3 cut(s) 404, 1172, 1514
PinAI ACCGGT 1 cut(s) 1177
PkrI GCNGC 5 cut(s) 212, 426, 486, 993, 1388
PleI GAGTC 4 cut(s) 233, 233, 265, 637
PpsI GAGTC 4 cut(s) 233, 233, 265, 637
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 4 cut(s) 622, 999, 1196, 1544
PspGI CCWGG 4 cut(s) 622, 999, 1196, 1544
PspN4I GGNNCC 2 cut(s) 110, 876
PspPI GGNCC 3 cut(s) 108, 418, 923
PspXI VCTCGAGB 1 cut(s) 254
PsrI GAACNNNNNNTAC 2 cut(s) 255, 287
PstI CTGCAG 1 cut(s) 1537
PsuI RGATCY 1 cut(s) 1254
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 8 cut(s) 93, 161, 368, 395, 509, 566, 584, 1266
RsaNI GTAC 8 cut(s) 92, 160, 367, 394, 508, 565, 583, 1265
SacI GAGCTC 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 1509
SaqAI TTAA 4 cut(s) 819, 887, 1061, 1496
SatI GCNGC 5 cut(s) 211, 425, 485, 992, 1387
Sau3AI GATC 6 cut(s) 168, 592, 1039, 1113, 1254, 1410
Sau96I GGNCC 3 cut(s) 108, 418, 923
ScaI AGTACT 2 cut(s) 368, 584
SchI GAGTC 4 cut(s) 233, 234, 266, 638
ScrFI CCNGG 5 cut(s) 624, 1001, 1198, 1418, 1546
SduI GDGCHC 2 cut(s) 456, 1492
SfaNI GCATC 3 cut(s) 838, 1156, 1247
SfcI CTRYAG 1 cut(s) 1533
Sfr274I CTCGAG 1 cut(s) 254
SfuI TTCGAA 1 cut(s) 1507
SinI GGWCC 3 cut(s) 108, 418, 923
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 254, 455
SmoI CTYRAG 2 cut(s) 254, 455
SphI GCATGC 1 cut(s) 1028
Sse9I AATT 8 cut(s) 299, 503, 634, 658, 761, 1062, 1405, 1572
SspMI CTAG 1 cut(s) 1350
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 5 cut(s) 622, 999, 1196, 1416, 1544
StyI CCWWGG 2 cut(s) 533, 1105
TaaI ACNGT 4 cut(s) 91, 923, 937, 979
TaiI ACGT 2 cut(s) 524, 1357
TaqI TCGA 7 cut(s) 198, 255, 309, 321, 356, 603, 1507
TaqII GACCGA 1 cut(s) 1437
TasI AATT 8 cut(s) 299, 503, 634, 658, 761, 1062, 1405, 1572
TatI WGTACW 3 cut(s) 366, 507, 582
TauI GCSGC 2 cut(s) 213, 427
TfiI GAWTC 3 cut(s) 404, 1172, 1514
Tru1I TTAA 4 cut(s) 819, 887, 1061, 1496
Tru9I TTAA 4 cut(s) 819, 887, 1061, 1496
TscAI CASTG 2 cut(s) 602, 862
TseFI GTSAC 1 cut(s) 790
TseI GCWGC 3 cut(s) 484, 991, 1386
Tsp45I GTSAC 1 cut(s) 790
TspDTI ATGAA 2 cut(s) 858, 1230
TspGWI ACGGA 4 cut(s) 26, 416, 1113, 1127
TspRI CASTG 2 cut(s) 602, 862
VpaK11BI GGWCC 3 cut(s) 108, 418, 923
XapI RAATTY 3 cut(s) 634, 761, 1405
XceI RCATGY 1 cut(s) 1028
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 1095
XmnI GAANNNNTTC 1 cut(s) 1513
XspI CTAG 1 cut(s) 1350
ZrmI AGTACT 2 cut(s) 368, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.