Rroxscaffold_2G00079060

TruB family pseudouridylate synthase (N terminal domain)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014138
Physical Location & Seq
Reverse (-)
2175186 .. 2178475
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00079060.1

Sequence Viewer

Length: 1521 bp
ATGGCTAAACCAGTCTCGCTGTCTCTTCTGTTCCTTCGCCCCGCCACACTCTCCCGCCTTCTCGCACACCCACCAACCCTCACCCTCACCCGCACCCGCACCAGAACCAACCCCCTTCTCTCCTCCCATACGCCGTCGTCGTTTTCAACCACCTCCACTCCATTCCCACTCCAGTACGACCTCATCATCAACCGCCCCACCCAGTCCTCGCTCGACCGCCGCCCCGCCCGCGCCGCCAAACCCCACTCATCCGGGTCGGAGTCCGATTCCCCCGAAAAGCCCAACGAATTGGGGTTCGACAACTGGCTGGACCAGAAGCTCGATCCGATGGAGAAGAAGAAGAGGAAGTATTACAACAAGCGGAGGAAGCGGATGTACGGCGGCGATTCGGAGGAGGAGGAGGAGAAGCGGCGGGAGGAGGAGAGGCTGGTGGAGCTGAAGCCGGAGGTGGTGGAGTTCAACACGTTGCATAAGAGAGAGGAGGAGCTCTACTTCTACGATACGTTTGCGTATCCGTGGGAGAAGGACAAGCATTACAAGATGGTGTACCAGCTGGAGAAGAAGTACTATCCCGACCAGTGTCTGGACAAAGCGTTTCTCGAACCCGGTGCAACGAATTGCAATCTGAGCAGGAAGAAGAAAGAGCGTGATGGTGATGATAAGGGACTGGTGTTTTTCGAGGGGAAGGGGGAGGGAGAATTGAGAGAACCGGCTGCTGCGACGAAGGATGTTGCAGAGAAGAAGGTGGAGGAGTTTTTCAAGTGTTTGAAGAAAGTGCCGAATGAAGTTAGTAATGAGGGGGAGCCGTATCTGTTGACAAGGAGTAGCGAGCTTCCTCCGAGGTGGGACGGGCCGTATGGAACTGTGGTTTTAGTGAACAAACCGAAAGGGTGGACCTCTTTTACAGTCTGTGGAAAGCTGCGGCGCCTAGTCAAAGTGAAGAAGGTAGGGCATGCTGGAACGCTTGATCCGATGGCAACTGGTTTGTTAATTGTATGTGTTGGTAAAGCTACTAAGGTTGTGGACAGCTATCAAGGTATGACCAAGGGTTACAGTGGAGTTTTCCGTCTGGGGGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTCATTCAGCGTGAGCCGTGGGAGCATATCAAAGATGAAGATATAAAGAAAACTGCTGCATCCTTTTGTGGAGAGATCTGGCAAGTACCTCCAATGTTTTCTGCCATCAAAGTTGGAGGTGAAAAGATGTATGAGAAGGCAAGAAGAGGAGAAAGCATTGAGCTCTCACCCAGGCGGATATCAATCTATCAGTTTGATATTGAGCGCAGCTTAGATAACAGACAAAATTTGATTTTCCGGGTTACTTGCTCTAAAGGGACATATATTAGGTCTCTATGTGCAGATTTGGGGAAAGCTCTCGGCAGTTGTGCTCATTTAACTGCTCTTCGGAGAGATTCAATTGGGGAATTTTCTGCAGATAATGCATGGGACTTCAAAGAGCTGGAAGATGCAATTACCAAAACTTATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TruB_C_2 TruB_N

Protein Analysis

506

Amino Acids

57.84

Weight (kDa)

8.9

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TruB_N PF01509 308 - 458 1.7e-53 TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2 PF16198 459 - 502 1.5e-09 tRNA pseudouridylate synthase B C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14460
fragaria_vesca FvH4_6g51780 FvH4_6g51780
malus_domestica MD17G1022100.v1.1
prunus_persica Prupe.3G294000_v2.0.a1
pyrus_communis pycom17g01920
rosa_chinensis RchiOBHm_Chr2g0173311
rosa_laevigata RLG00000022178
rosa_multiflora Rmu_sc0010961.1_g000005
rosa_roxburghii Rroxscaffold_2G00079060
rosa_rugosa Rorug02G0567200 Rorug02G0567300
rosa_samantha Rh2AG647600 Rh2BG658100 Rh2CG623500 Rh2DG671900
rosa_wichuraiana Rw2G053160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 924
AccB7I CCANNNNNTGG 1 cut(s) 583
AccII CGCG 1 cut(s) 231
AclWI GGATC 2 cut(s) 317, 962
AcsI RAATTY 2 cut(s) 1333, 1454
AcuI CTGAAG 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 925
AfaI GTAC 5 cut(s) 176, 377, 548, 566, 1194
AfiI CCNNNNNNNGG 2 cut(s) 583, 1072
AflIII ACRYGT 1 cut(s) 462
AgeI ACCGGT 1 cut(s) 1105
AgsI TTSAA 7 cut(s) 147, 460, 760, 769, 1086, 1446, 1483
AjnI CCWGG 1 cut(s) 1277
AjuI GAANNNNNNNTTGG 1 cut(s) 1499
AloI GAACNNNNNNTCC 2 cut(s) 952, 984
Alw21I GWGCWC 3 cut(s) 489, 1272, 1420
Alw26I GTCTC 3 cut(s) 19, 27, 1383
AlwI GGATC 2 cut(s) 317, 962
AlwNI CAGNNNCTG 1 cut(s) 583
AoxI GGCC 1 cut(s) 851
ApeKI GCWGC 5 cut(s) 713, 716, 919, 1163, 1314
ApoI RAATTY 2 cut(s) 1333, 1454
AsiGI ACCGGT 1 cut(s) 1105
AspLEI GCGC 3 cut(s) 233, 927, 1314
AspS9I GGNCC 3 cut(s) 310, 851, 894
AsuC2I CCSGG 3 cut(s) 253, 606, 1346
AsuHPI GGTGA 6 cut(s) 73, 79, 665, 1095, 1238, 1266
AvaII GGWCC 2 cut(s) 310, 894
BanI GGYRCC 1 cut(s) 924
BanII GRGCYC 2 cut(s) 489, 1272
BarI GAAGNNNNNNTAC 2 cut(s) 359, 391
Bbv12I GWGCWC 3 cut(s) 489, 1272, 1420
BbvI GCAGC 5 cut(s) 700, 703, 906, 1150, 1326
BccI CCATC 5 cut(s) 322, 535, 644, 967, 1220
BceAI ACGGC 5 cut(s) 118, 394, 790, 838, 1108
BcgI CGANNNNNNTGC 4 cut(s) 488, 522, 590, 624
BciT130I CCWGG 1 cut(s) 1279
BciVI GTATCC 1 cut(s) 522
BcnI CCSGG 3 cut(s) 253, 606, 1346
BcoDI GTCTC 3 cut(s) 19, 27, 1383
BfaI CTAG 2 cut(s) 929, 1519
BfmI CTRYAG 1 cut(s) 1461
BfoI RGCGCY 1 cut(s) 928
BfuI GTATCC 1 cut(s) 522
BglII AGATCT 1 cut(s) 1182
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 4 cut(s) 253, 606, 1279, 1346
Bme18I GGWCC 2 cut(s) 310, 894
BmgT120I GGNCC 3 cut(s) 310, 851, 894
BmiI GGNNCC 2 cut(s) 804, 926
BmrFI CCNGG 4 cut(s) 253, 606, 1279, 1346
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 3 cut(s) 1084, 1175, 1486
BmuI ACTGGG 1 cut(s) 196
BoxI GACNNNNGTC 1 cut(s) 579
BpmI CTGGAG 2 cut(s) 155, 575
BpuMI CCSGG 3 cut(s) 253, 606, 1346
BsaBI GATNNNNATC 1 cut(s) 1289
BsaHI GRCGYC 1 cut(s) 925
BsaI GGTCTC 1 cut(s) 1383
BsaJI CCNNGG 5 cut(s) 515, 839, 1044, 1124, 1277
BsaWI WCCGGW 1 cut(s) 1105
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 548, 578
Bsc4I CCNNNNNNNGG 2 cut(s) 583, 1072
Bse118I RCCGGY 2 cut(s) 709, 1105
Bse1I ACTGG 7 cut(s) 11, 172, 202, 308, 577, 672, 985
Bse8I GATNNNNATC 1 cut(s) 1289
BseBI CCWGG 1 cut(s) 1279
BseDI CCNNGG 5 cut(s) 515, 839, 1044, 1124, 1277
BseGI GGATG 5 cut(s) 248, 378, 733, 1099, 1166
BseJI GATNNNNATC 1 cut(s) 1289
BseLI CCNNNNNNNGG 2 cut(s) 583, 1072
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 7 cut(s) 11, 172, 202, 308, 577, 672, 985
BseXI GCAGC 5 cut(s) 700, 703, 906, 1150, 1326
BsgI GTGCAG 1 cut(s) 1407
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 853
BshNI GGYRCC 1 cut(s) 924
BshTI ACCGGT 1 cut(s) 1105
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 3 cut(s) 489, 1272, 1420
BsiSI CCGG 6 cut(s) 252, 443, 606, 710, 1106, 1345
BslFI GGGAC 4 cut(s) 678, 860, 1378, 1490
BslI CCNNNNNNNGG 2 cut(s) 583, 1072
BsmAI GTCTC 3 cut(s) 19, 27, 1383
BsmFI GGGAC 4 cut(s) 678, 860, 1378, 1490
BsnI GGCC 1 cut(s) 853
Bso31I GGTCTC 1 cut(s) 1383
Bsp1286I GDGCHC 3 cut(s) 489, 1272, 1420
Bsp143I GATC 3 cut(s) 322, 967, 1182
BspANI GGCC 1 cut(s) 853
BspCNI CTCAG 1 cut(s) 618
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 804, 926
BspMAI CTGCAG 1 cut(s) 1465
BspPI GGATC 2 cut(s) 317, 962
BspQI GCTCTTC 1 cut(s) 1437
BspT107I GGYRCC 1 cut(s) 924
BspTNI GGTCTC 1 cut(s) 1383
BsrFI RCCGGY 2 cut(s) 709, 1105
BsrI ACTGG 7 cut(s) 11, 172, 202, 308, 577, 672, 985
BssAI RCCGGY 2 cut(s) 709, 1105
BssECI CCNNGG 5 cut(s) 515, 839, 1044, 1124, 1277
BssMI GATC 3 cut(s) 322, 967, 1182
BssNI GRCGYC 1 cut(s) 925
BssT1I CCWWGG 1 cut(s) 1044
Bst2UI CCWGG 1 cut(s) 1279
Bst4CI ACNGT 3 cut(s) 865, 907, 1055
Bst6I CTCTTC 4 cut(s) 30, 335, 1246, 1437
BstACI GRCGYC 1 cut(s) 925
BstAPI GCANNNNNTGC 1 cut(s) 1469
BstC8I GCNNGC 3 cut(s) 229, 830, 954
BstDEI CTNAG 3 cut(s) 626, 1014, 1318
BstDSI CCRYGG 2 cut(s) 515, 1124
BstF5I GGATG 5 cut(s) 248, 378, 733, 1099, 1166
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 928
BstHHI GCGC 3 cut(s) 233, 927, 1314
BstKTI GATC 3 cut(s) 325, 970, 1185
BstMAI GTCTC 3 cut(s) 19, 27, 1383
BstMBI GATC 3 cut(s) 322, 967, 1182
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 7 cut(s) 228, 233, 367, 433, 627, 1129, 1469
BstNI CCWGG 1 cut(s) 1279
BstNSI RCATGY 1 cut(s) 956
BstPAI GACNNNNGTC 1 cut(s) 579
BstSCI CCNGG 4 cut(s) 251, 604, 1277, 1344
BstSFI CTRYAG 1 cut(s) 1461
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 5 cut(s) 700, 703, 906, 1150, 1326
BstX2I RGATCY 1 cut(s) 1182
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 1 cut(s) 1182
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 1 cut(s) 853
BtgI CCRYGG 2 cut(s) 515, 1124
BtsCI GGATG 5 cut(s) 248, 378, 733, 1099, 1166
BtsIMutI CAGTG 2 cut(s) 584, 1060
Cac8I GCNNGC 3 cut(s) 229, 830, 954
CaiI CAGNNNCTG 1 cut(s) 583
CfoI GCGC 3 cut(s) 233, 927, 1314
Cfr10I RCCGGY 2 cut(s) 709, 1105
Cfr13I GGNCC 3 cut(s) 310, 851, 894
Csp6I GTAC 5 cut(s) 175, 376, 547, 565, 1193
CspAI ACCGGT 1 cut(s) 1105
CviAII CATG 2 cut(s) 953, 1473
CviQI GTAC 5 cut(s) 175, 376, 547, 565, 1193
DdeI CTNAG 3 cut(s) 626, 1014, 1318
DinI GGCGCC 1 cut(s) 926
DpnI GATC 3 cut(s) 324, 969, 1184
DpnII GATC 3 cut(s) 322, 967, 1182
Eam1104I CTCTTC 4 cut(s) 30, 335, 1246, 1437
EarI CTCTTC 4 cut(s) 30, 335, 1246, 1437
EciI GGCGGA 1 cut(s) 1297
Ecl136II GAGCTC 2 cut(s) 487, 1270
Eco130I CCWWGG 1 cut(s) 1044
Eco24I GRGCYC 2 cut(s) 489, 1272
Eco31I GGTCTC 1 cut(s) 1383
Eco32I GATATC 1 cut(s) 1287
Eco47I GGWCC 2 cut(s) 310, 894
Eco53kI GAGCTC 2 cut(s) 487, 1270
Eco57I CTGAAG 1 cut(s) 458
EcoICRI GAGCTC 2 cut(s) 487, 1270
EcoRII CCWGG 1 cut(s) 1277
EcoRV GATATC 1 cut(s) 1287
EcoT14I CCWWGG 1 cut(s) 1044
EcoT22I ATGCAT 1 cut(s) 1474
EcoT38I GRGCYC 2 cut(s) 489, 1272
EgeI GGCGCC 1 cut(s) 926
EheI GGCGCC 1 cut(s) 926
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 2 cut(s) 956, 1476
FaqI GGGAC 4 cut(s) 678, 860, 1378, 1490
FatI CATG 2 cut(s) 952, 1472
FauI CCCGC 7 cut(s) 49, 62, 98, 104, 232, 236, 405
FokI GGATG 5 cut(s) 235, 385, 740, 1106, 1153
FriOI GRGCYC 2 cut(s) 489, 1272
FspBI CTAG 2 cut(s) 929, 1519
GlaI GCGC 3 cut(s) 232, 926, 1313
GsuI CTGGAG 2 cut(s) 155, 575
HaeII RGCGCY 1 cut(s) 928
HaeIII GGCC 1 cut(s) 853
HapII CCGG 6 cut(s) 252, 443, 606, 710, 1106, 1345
HhaI GCGC 3 cut(s) 233, 927, 1314
Hin1I GRCGYC 1 cut(s) 925
Hin1II CATG 2 cut(s) 956, 1476
Hin6I GCGC 3 cut(s) 231, 925, 1312
HinP1I GCGC 3 cut(s) 231, 925, 1312
HincII GTYRAC 1 cut(s) 816
HindII GTYRAC 1 cut(s) 816
HinfI GANTC 5 cut(s) 260, 266, 386, 1100, 1442
HpaII CCGG 6 cut(s) 252, 443, 606, 710, 1106, 1345
HphI GGTGA 6 cut(s) 73, 79, 665, 1095, 1238, 1266
Hpy166II GTNNAC 5 cut(s) 547, 816, 877, 894, 1024
Hpy188I TCNGA 8 cut(s) 259, 265, 327, 391, 627, 840, 972, 1437
Hpy188III TCNNGA 3 cut(s) 572, 584, 599
Hpy8I GTNNAC 5 cut(s) 547, 816, 877, 894, 1024
Hpy99I CGWCG 3 cut(s) 139, 142, 724
HpyAV CCTTC 9 cut(s) 44, 68, 125, 517, 679, 718, 736, 937, 1237
HpyCH4III ACNGT 3 cut(s) 865, 907, 1055
HpyCH4IV ACGT 2 cut(s) 464, 503
HpyCH4V TGCA 9 cut(s) 469, 611, 621, 734, 1166, 1388, 1463, 1472, 1499
HpyF10VI GCNNNNNNNGC 7 cut(s) 228, 233, 367, 433, 627, 1129, 1469
HpyF3I CTNAG 3 cut(s) 626, 1014, 1318
HpySE526I ACGT 2 cut(s) 464, 503
Hsp92I GRCGYC 1 cut(s) 925
Hsp92II CATG 2 cut(s) 956, 1476
HspAI GCGC 3 cut(s) 231, 925, 1312
KasI GGCGCC 1 cut(s) 924
Kzo9I GATC 3 cut(s) 322, 967, 1182
LguI GCTCTTC 1 cut(s) 1437
LmnI GCTCC 4 cut(s) 433, 484, 802, 1129
Lsp1109I GCAGC 5 cut(s) 700, 703, 906, 1150, 1326
LweI GCATC 3 cut(s) 1084, 1175, 1486
MaeI CTAG 2 cut(s) 929, 1519
MaeII ACGT 2 cut(s) 464, 503
MaeIII GTNAC 2 cut(s) 1049, 1348
MalI GATC 3 cut(s) 324, 969, 1184
MboI GATC 3 cut(s) 322, 967, 1182
MfeI CAATTG 1 cut(s) 1446
MflI RGATCY 1 cut(s) 1182
MhlI GDGCHC 3 cut(s) 489, 1272, 1420
MluCI AATT 8 cut(s) 287, 616, 698, 990, 1333, 1446, 1454, 1500
Mly113I GGCGCC 1 cut(s) 925
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 237, 1201
Mph1103I ATGCAT 1 cut(s) 1474
MseI TTAA 2 cut(s) 989, 1424
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 6 cut(s) 252, 443, 606, 710, 1106, 1345
MspR9I CCNGG 4 cut(s) 253, 606, 1279, 1346
MunI CAATTG 1 cut(s) 1446
MvaI CCWGG 1 cut(s) 1279
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 7 cut(s) 228, 233, 367, 433, 627, 1129, 1469
NarI GGCGCC 1 cut(s) 925
NciI CCSGG 3 cut(s) 253, 606, 1346
NdeII GATC 3 cut(s) 322, 967, 1182
NlaIII CATG 2 cut(s) 956, 1476
NlaIV GGNNCC 2 cut(s) 804, 926
NmeAIII GCCGAG 1 cut(s) 1386
NsiI ATGCAT 1 cut(s) 1474
NspI RCATGY 1 cut(s) 956
PaeI GCATGC 1 cut(s) 956
PciSI GCTCTTC 1 cut(s) 1437
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 968
PfeI GAWTC 4 cut(s) 266, 386, 1100, 1442
PflMI CCANNNNNTGG 1 cut(s) 583
PinAI ACCGGT 1 cut(s) 1105
PleI GAGTC 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 268
PshAI GACNNNNGTC 1 cut(s) 579
Psp124BI GAGCTC 2 cut(s) 489, 1272
Psp6I CCWGG 1 cut(s) 1277
PspGI CCWGG 1 cut(s) 1277
PspN4I GGNNCC 2 cut(s) 804, 926
PspPI GGNCC 3 cut(s) 310, 851, 894
PstI CTGCAG 1 cut(s) 1465
PstNI CAGNNNCTG 1 cut(s) 583
PsuI RGATCY 1 cut(s) 1182
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 5 cut(s) 176, 377, 548, 566, 1194
RsaNI GTAC 5 cut(s) 175, 376, 547, 565, 1193
SacI GAGCTC 2 cut(s) 489, 1272
SapI GCTCTTC 1 cut(s) 1437
SaqAI TTAA 2 cut(s) 989, 1424
Sau3AI GATC 3 cut(s) 322, 967, 1182
Sau96I GGNCC 3 cut(s) 310, 851, 894
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 4 cut(s) 253, 606, 1279, 1346
SduI GDGCHC 3 cut(s) 489, 1272, 1420
SfaNI GCATC 3 cut(s) 1084, 1175, 1486
SfcI CTRYAG 1 cut(s) 1461
SfoI GGCGCC 1 cut(s) 926
SinI GGWCC 2 cut(s) 310, 894
SphI GCATGC 1 cut(s) 956
Sse9I AATT 8 cut(s) 287, 616, 698, 990, 1333, 1446, 1454, 1500
SspDI GGCGCC 1 cut(s) 924
SspMI CTAG 2 cut(s) 929, 1519
SstI GAGCTC 2 cut(s) 489, 1272
StyD4I CCNGG 4 cut(s) 251, 604, 1277, 1344
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 3 cut(s) 865, 907, 1055
TaiI ACGT 2 cut(s) 467, 506
TaqI TCGA 5 cut(s) 213, 297, 321, 600, 678
TasI AATT 8 cut(s) 287, 616, 698, 990, 1333, 1446, 1454, 1500
TatI WGTACW 1 cut(s) 564
TauI GCSGC 5 cut(s) 222, 236, 384, 412, 925
TfiI GAWTC 4 cut(s) 266, 386, 1100, 1442
Tru1I TTAA 2 cut(s) 989, 1424
Tru9I TTAA 2 cut(s) 989, 1424
TscAI CASTG 2 cut(s) 584, 1060
TseI GCWGC 5 cut(s) 713, 716, 919, 1163, 1314
TspDTI ATGAA 2 cut(s) 798, 1158
TspGWI ACGGA 2 cut(s) 504, 1055
TspRI CASTG 2 cut(s) 584, 1060
Van91I CCANNNNNTGG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 310, 894
XapI RAATTY 2 cut(s) 1333, 1454
XceI RCATGY 1 cut(s) 956
XspI CTAG 2 cut(s) 929, 1519
ZrmI AGTACT 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 1474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.