RLG00000022178

TruB family pseudouridylate synthase (N terminal domain)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014138
Physical Location & Seq
Forward (+)
81550573 .. 81553414
2842 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022178

Sequence Viewer

Length: 1512 bp
ATGGCTAAACCAGTCTCTCTGTCTCTTCTCTTCCTTCGCCCCGCCACACTCTCCCGCCTTCTCCCACACCCACCAACCCTCACCCTCACCCGCACCAGAACCAACCCCCTTCTCTCCTCCCATACGCCGTCGTTTTCAACCACCTCCACTCCATTCCCACTCCAGTACGACCTCATCATCAACCGCCCCACCCAGTCGTCGCTCGACCGCCGCCCCGCCCGCGCCGCCAAACCCGACTCATCCGGGTCGGAGTCCGATTCCCCCGAAAAGCCCAACGAATTGGGGTTCGACAACTGGCTTGACCAGAAGCTCGATCCGATGGAGAAGAAGAAGAGGAAGTATTATAACAAGCGGAGGAAGCGGATGTACGGCGGCGATTCGGAGGAGGAGGAGGAGAAGCGGCGGGAGGAGGAGAGGCTTGTGGAGCTGAAGCCGGAGGTGGTGGAGTTCAACACGTTGCATAAGAGAGAGGAGGAGCTCTACTTCTACGATACGTTTGCGTATCCGTGGGAGAAGGACAAGCATTACAAGATGGTGTACCAGCTGGAGAAGAAGTACTATCCGGACCAGTGTCTGGACAAAGCGTTTCTGGAACCCGGTGCAACGAATTGCAATCTGAGTAGGAAGAAGAAAGAGCGTGATGGTGATGATAAGGGAATGGTGTTTTTCGAGGGGAAGGGGGAGGGAGAATCGAGAGAACCGGCTGCTGCGACGAAGGATGTTGCAGAGAAGAAGGTGGAGGAGTTTTTCAAGTGTTTGAAGAAAGTGCCGAATGAAGTTAGTAATGAGGGGGAGCCGTATCTGTTGACAAGGAGTAGCGAGCTGCCTCCGAGGTGGGACGGGCCGTTTGGGACTGTGGTTTTGGTGAACAAACCGAAAGGGTGGACCTCTTTTACAGTTTGTGGAAAGCTGCGGCGCCTAGTCAGAGTGAAGAAGGTAGGGCATGCTGGAACGCTTGATCCAATGGCAACTGGTTTGTTAATTGTATGTGTTGGTAAAGCTACTAAGGTTGTGGACAGCTATCAAGGTATGACCAAGGGTTACAGTGGAGTTTTCCGTCTGGGGGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTCATTCAGCGTGAGCCGTGGGAGCATATCAAAGATGAAGATATAAAGAAAACTGCTGCATCCTTTTGTGGAGAGATCTGGCAAGTACCTCCAATGTTTTCTGCCATCAAAGTTGGAGGAGAAAAGATGTATGAGAAGGCAAGAAGAGGAGAAAGCATTGAGCTCTCTCCCAGACGGATATCAATCTATCAGTTTGACATTGAGCGCAGCTTAGATGACAGACAAAATTTGATTTTCCGGGTTACTTGCTCTAAAGGGACATATATTAGGTCTCTTTGCGCAGATTTGGGGAAAGCTCTCGGCAGTTGTGCTCATTTAACTGCTCTCCGGAGAGATTCAATTGGGGAATATTCTGCAGATAATGCATGGGACTTCAAAGAGCTGGAAGATGCAATTACCAAAACTTATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TruB_C_2 TruB_N

Protein Analysis

504

Amino Acids

57.52

Weight (kDa)

8.68

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TruB_N PF01509 305 - 455 3e-53 TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2 PF16198 456 - 499 2.1e-09 tRNA pseudouridylate synthase B C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14460
fragaria_vesca FvH4_6g51780 FvH4_6g51780
malus_domestica MD17G1022100.v1.1
prunus_persica Prupe.3G294000_v2.0.a1
pyrus_communis pycom17g01920
rosa_chinensis RchiOBHm_Chr2g0173311
rosa_laevigata RLG00000022178
rosa_multiflora Rmu_sc0010961.1_g000005
rosa_roxburghii Rroxscaffold_2G00079060
rosa_rugosa Rorug02G0567200 Rorug02G0567300
rosa_samantha Rh2AG647600 Rh2BG658100 Rh2CG623500 Rh2DG671900
rosa_wichuraiana Rw2G053160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc16I TGCGCA 1 cut(s) 1378
AccB1I GGYRCC 1 cut(s) 915
AccB7I CCANNNNNTGG 1 cut(s) 574
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 2 cut(s) 562, 1425
AclWI GGATC 2 cut(s) 308, 953
AcsI RAATTY 1 cut(s) 1324
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 916
AfaI GTAC 5 cut(s) 167, 368, 539, 557, 1185
AfiI CCNNNNNNNGG 2 cut(s) 574, 1063
AflIII ACRYGT 1 cut(s) 453
AgeI ACCGGT 1 cut(s) 1096
AgsI TTSAA 7 cut(s) 138, 451, 751, 760, 1077, 1437, 1474
AjuI GAANNNNNNNTTGG 1 cut(s) 1490
AloI GAACNNNNNNTCC 2 cut(s) 943, 975
Alw21I GWGCWC 3 cut(s) 480, 1263, 1411
Alw26I GTCTC 3 cut(s) 19, 27, 1374
AlwI GGATC 2 cut(s) 308, 953
AlwNI CAGNNNCTG 1 cut(s) 574
Aor13HI TCCGGA 2 cut(s) 562, 1425
AoxI GGCC 1 cut(s) 842
ApeKI GCWGC 6 cut(s) 704, 707, 823, 910, 1154, 1305
ApoI RAATTY 1 cut(s) 1324
ArsI GACNNNNNNTTYG 4 cut(s) 830, 844, 862, 876
AsiGI ACCGGT 1 cut(s) 1096
AspLEI GCGC 4 cut(s) 224, 918, 1305, 1379
AspS9I GGNCC 3 cut(s) 565, 842, 885
AsuC2I CCSGG 3 cut(s) 244, 597, 1337
AsuHPI GGTGA 5 cut(s) 73, 79, 656, 877, 1086
AvaII GGWCC 2 cut(s) 565, 885
BanI GGYRCC 1 cut(s) 915
BanII GRGCYC 2 cut(s) 480, 1263
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
Bbv12I GWGCWC 3 cut(s) 480, 1263, 1411
BbvI GCAGC 6 cut(s) 691, 694, 810, 897, 1141, 1317
BccI CCATC 4 cut(s) 313, 526, 635, 1211
BceAI ACGGC 5 cut(s) 112, 385, 781, 829, 1099
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciVI GTATCC 1 cut(s) 513
BcnI CCSGG 3 cut(s) 244, 597, 1337
BcoDI GTCTC 3 cut(s) 19, 27, 1374
BfaI CTAG 2 cut(s) 920, 1510
BfmI CTRYAG 1 cut(s) 1452
BfoI RGCGCY 1 cut(s) 919
BfuI GTATCC 1 cut(s) 513
BglII AGATCT 1 cut(s) 1173
BmcAI AGTACT 1 cut(s) 557
Bme1390I CCNGG 3 cut(s) 244, 597, 1337
Bme18I GGWCC 2 cut(s) 565, 885
BmgT120I GGNCC 3 cut(s) 565, 842, 885
BmiI GGNNCC 3 cut(s) 594, 795, 917
BmrFI CCNGG 3 cut(s) 244, 597, 1337
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 3 cut(s) 1075, 1166, 1477
BmuI ACTGGG 1 cut(s) 187
BoxI GACNNNNGTC 1 cut(s) 570
BpmI CTGGAG 2 cut(s) 146, 566
BpuMI CCSGG 3 cut(s) 244, 597, 1337
BsaBI GATNNNNATC 1 cut(s) 1280
BsaHI GRCGYC 1 cut(s) 916
BsaI GGTCTC 1 cut(s) 1374
BsaJI CCNNGG 4 cut(s) 506, 830, 1035, 1115
BsaWI WCCGGW 3 cut(s) 562, 1096, 1425
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 539, 569
Bsc4I CCNNNNNNNGG 2 cut(s) 574, 1063
Bse118I RCCGGY 2 cut(s) 700, 1096
Bse1I ACTGG 6 cut(s) 11, 163, 193, 299, 568, 976
Bse8I GATNNNNATC 1 cut(s) 1280
BseAI TCCGGA 2 cut(s) 562, 1425
BseDI CCNNGG 4 cut(s) 506, 830, 1035, 1115
BseGI GGATG 5 cut(s) 239, 369, 724, 1090, 1157
BseJI GATNNNNATC 1 cut(s) 1280
BseLI CCNNNNNNNGG 2 cut(s) 574, 1063
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 6 cut(s) 11, 163, 193, 299, 568, 976
BseXI GCAGC 6 cut(s) 691, 694, 810, 897, 1141, 1317
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 844
BshNI GGYRCC 1 cut(s) 915
BshTI ACCGGT 1 cut(s) 1096
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 3 cut(s) 480, 1263, 1411
BsiSI CCGG 8 cut(s) 243, 434, 563, 597, 701, 1097, 1336, 1426
BslFI GGGAC 4 cut(s) 851, 865, 1369, 1481
BslI CCNNNNNNNGG 2 cut(s) 574, 1063
BsmAI GTCTC 3 cut(s) 19, 27, 1374
BsmFI GGGAC 4 cut(s) 851, 865, 1369, 1481
BsnI GGCC 1 cut(s) 844
Bso31I GGTCTC 1 cut(s) 1374
Bsp1286I GDGCHC 3 cut(s) 480, 1263, 1411
Bsp13I TCCGGA 2 cut(s) 562, 1425
Bsp143I GATC 3 cut(s) 313, 958, 1173
BspANI GGCC 1 cut(s) 844
BspCNI CTCAG 1 cut(s) 609
BspEI TCCGGA 2 cut(s) 562, 1425
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 3 cut(s) 594, 795, 917
BspMAI CTGCAG 1 cut(s) 1456
BspPI GGATC 2 cut(s) 308, 953
BspT107I GGYRCC 1 cut(s) 915
BspTNI GGTCTC 1 cut(s) 1374
BsrFI RCCGGY 2 cut(s) 700, 1096
BsrI ACTGG 6 cut(s) 11, 163, 193, 299, 568, 976
BssAI RCCGGY 2 cut(s) 700, 1096
BssECI CCNNGG 4 cut(s) 506, 830, 1035, 1115
BssMI GATC 3 cut(s) 313, 958, 1173
BssNI GRCGYC 1 cut(s) 916
BssT1I CCWWGG 1 cut(s) 1035
Bst4CI ACNGT 3 cut(s) 856, 898, 1046
Bst6I CTCTTC 4 cut(s) 30, 35, 326, 1237
BstACI GRCGYC 1 cut(s) 916
BstAPI GCANNNNNTGC 1 cut(s) 1460
BstC8I GCNNGC 3 cut(s) 220, 821, 945
BstDEI CTNAG 3 cut(s) 617, 1005, 1309
BstDSI CCRYGG 2 cut(s) 506, 1115
BstF5I GGATG 5 cut(s) 239, 369, 724, 1090, 1157
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 919
BstHHI GCGC 4 cut(s) 224, 918, 1305, 1379
BstKTI GATC 3 cut(s) 316, 961, 1176
BstMAI GTCTC 3 cut(s) 19, 27, 1374
BstMBI GATC 3 cut(s) 313, 958, 1173
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 219, 224, 358, 424, 1120, 1460
BstNSI RCATGY 1 cut(s) 947
BstPAI GACNNNNGTC 1 cut(s) 570
BstSCI CCNGG 3 cut(s) 242, 595, 1335
BstSFI CTRYAG 1 cut(s) 1452
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 6 cut(s) 691, 694, 810, 897, 1141, 1317
BstX2I RGATCY 1 cut(s) 1173
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 1173
BsuI GTATCC 1 cut(s) 513
BsuRI GGCC 1 cut(s) 844
BtgI CCRYGG 2 cut(s) 506, 1115
BtsCI GGATG 5 cut(s) 239, 369, 724, 1090, 1157
BtsIMutI CAGTG 2 cut(s) 575, 1051
Cac8I GCNNGC 3 cut(s) 220, 821, 945
CaiI CAGNNNCTG 1 cut(s) 574
CfoI GCGC 4 cut(s) 224, 918, 1305, 1379
Cfr10I RCCGGY 2 cut(s) 700, 1096
Cfr13I GGNCC 3 cut(s) 565, 842, 885
Csp6I GTAC 5 cut(s) 166, 367, 538, 556, 1184
CspAI ACCGGT 1 cut(s) 1096
CviAII CATG 2 cut(s) 944, 1464
CviQI GTAC 5 cut(s) 166, 367, 538, 556, 1184
DdeI CTNAG 3 cut(s) 617, 1005, 1309
DinI GGCGCC 1 cut(s) 917
DpnI GATC 3 cut(s) 315, 960, 1175
DpnII GATC 3 cut(s) 313, 958, 1173
Eam1104I CTCTTC 4 cut(s) 30, 35, 326, 1237
EarI CTCTTC 4 cut(s) 30, 35, 326, 1237
Ecl136II GAGCTC 2 cut(s) 478, 1261
Eco130I CCWWGG 1 cut(s) 1035
Eco24I GRGCYC 2 cut(s) 480, 1263
Eco31I GGTCTC 1 cut(s) 1374
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 2 cut(s) 565, 885
Eco53kI GAGCTC 2 cut(s) 478, 1261
Eco57I CTGAAG 1 cut(s) 449
EcoICRI GAGCTC 2 cut(s) 478, 1261
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 1 cut(s) 1035
EcoT22I ATGCAT 1 cut(s) 1465
EcoT38I GRGCYC 2 cut(s) 480, 1263
EgeI GGCGCC 1 cut(s) 917
EheI GGCGCC 1 cut(s) 917
ErhI CCWWGG 1 cut(s) 1035
FaeI CATG 2 cut(s) 947, 1467
FaqI GGGAC 4 cut(s) 851, 865, 1369, 1481
FatI CATG 2 cut(s) 943, 1463
FauI CCCGC 6 cut(s) 49, 62, 98, 223, 227, 396
FokI GGATG 5 cut(s) 226, 376, 731, 1097, 1144
FriOI GRGCYC 2 cut(s) 480, 1263
FspBI CTAG 2 cut(s) 920, 1510
FspI TGCGCA 1 cut(s) 1378
GlaI GCGC 4 cut(s) 223, 917, 1304, 1378
GsuI CTGGAG 2 cut(s) 146, 566
HaeII RGCGCY 1 cut(s) 919
HaeIII GGCC 1 cut(s) 844
HapII CCGG 8 cut(s) 243, 434, 563, 597, 701, 1097, 1336, 1426
HhaI GCGC 4 cut(s) 224, 918, 1305, 1379
Hin1I GRCGYC 1 cut(s) 916
Hin1II CATG 2 cut(s) 947, 1467
Hin6I GCGC 4 cut(s) 222, 916, 1303, 1377
HinP1I GCGC 4 cut(s) 222, 916, 1303, 1377
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HinfI GANTC 7 cut(s) 236, 251, 257, 377, 689, 1091, 1433
HpaII CCGG 8 cut(s) 243, 434, 563, 597, 701, 1097, 1336, 1426
HphI GGTGA 5 cut(s) 73, 79, 656, 877, 1086
Hpy166II GTNNAC 5 cut(s) 538, 807, 868, 885, 1015
Hpy188I TCNGA 7 cut(s) 250, 256, 318, 382, 618, 831, 926
Hpy188III TCNNGA 5 cut(s) 563, 575, 590, 693, 1426
Hpy8I GTNNAC 5 cut(s) 538, 807, 868, 885, 1015
Hpy99I CGWCG 3 cut(s) 133, 202, 715
HpyAV CCTTC 9 cut(s) 44, 68, 119, 508, 670, 709, 727, 928, 1228
HpyCH4III ACNGT 3 cut(s) 856, 898, 1046
HpyCH4IV ACGT 2 cut(s) 455, 494
HpyCH4V TGCA 8 cut(s) 460, 602, 612, 725, 1157, 1454, 1463, 1490
HpyF10VI GCNNNNNNNGC 6 cut(s) 219, 224, 358, 424, 1120, 1460
HpyF3I CTNAG 3 cut(s) 617, 1005, 1309
HpySE526I ACGT 2 cut(s) 455, 494
Hsp92I GRCGYC 1 cut(s) 916
Hsp92II CATG 2 cut(s) 947, 1467
HspAI GCGC 4 cut(s) 222, 916, 1303, 1377
KasI GGCGCC 1 cut(s) 915
Kpn2I TCCGGA 2 cut(s) 562, 1425
Kzo9I GATC 3 cut(s) 313, 958, 1173
LmnI GCTCC 4 cut(s) 424, 475, 793, 1120
Lsp1109I GCAGC 6 cut(s) 691, 694, 810, 897, 1141, 1317
LweI GCATC 3 cut(s) 1075, 1166, 1477
MaeI CTAG 2 cut(s) 920, 1510
MaeII ACGT 2 cut(s) 455, 494
MaeIII GTNAC 2 cut(s) 1040, 1339
MalI GATC 3 cut(s) 315, 960, 1175
MboI GATC 3 cut(s) 313, 958, 1173
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 1 cut(s) 1173
MhlI GDGCHC 3 cut(s) 480, 1263, 1411
MluCI AATT 6 cut(s) 278, 607, 981, 1324, 1437, 1491
Mly113I GGCGCC 1 cut(s) 916
MlyI GAGTC 2 cut(s) 230, 260
MmeI TCCRAC 2 cut(s) 228, 1192
Mph1103I ATGCAT 1 cut(s) 1465
MroI TCCGGA 2 cut(s) 562, 1425
MseI TTAA 2 cut(s) 980, 1415
MspA1I CMGCKG 1 cut(s) 544
MspI CCGG 8 cut(s) 243, 434, 563, 597, 701, 1097, 1336, 1426
MspR9I CCNGG 3 cut(s) 244, 597, 1337
MunI CAATTG 1 cut(s) 1437
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 6 cut(s) 219, 224, 358, 424, 1120, 1460
NarI GGCGCC 1 cut(s) 916
NciI CCSGG 3 cut(s) 244, 597, 1337
NdeII GATC 3 cut(s) 313, 958, 1173
NlaIII CATG 2 cut(s) 947, 1467
NlaIV GGNNCC 3 cut(s) 594, 795, 917
NmeAIII GCCGAG 1 cut(s) 1377
NsbI TGCGCA 1 cut(s) 1378
NsiI ATGCAT 1 cut(s) 1465
NspI RCATGY 1 cut(s) 947
PaeI GCATGC 1 cut(s) 947
PfeI GAWTC 5 cut(s) 257, 377, 689, 1091, 1433
PflMI CCANNNNNTGG 1 cut(s) 574
PinAI ACCGGT 1 cut(s) 1096
PleI GAGTC 2 cut(s) 230, 259
PluTI GGCGCC 1 cut(s) 919
PpsI GAGTC 2 cut(s) 230, 259
PshAI GACNNNNGTC 1 cut(s) 570
PsiI TTATAA 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 480, 1263
PspN4I GGNNCC 3 cut(s) 594, 795, 917
PspPI GGNCC 3 cut(s) 565, 842, 885
PstI CTGCAG 1 cut(s) 1456
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 1173
PvuII CAGCTG 1 cut(s) 544
RsaI GTAC 5 cut(s) 167, 368, 539, 557, 1185
RsaNI GTAC 5 cut(s) 166, 367, 538, 556, 1184
SacI GAGCTC 2 cut(s) 480, 1263
SaqAI TTAA 2 cut(s) 980, 1415
Sau3AI GATC 3 cut(s) 313, 958, 1173
Sau96I GGNCC 3 cut(s) 565, 842, 885
ScaI AGTACT 1 cut(s) 557
SchI GAGTC 2 cut(s) 230, 260
ScrFI CCNGG 3 cut(s) 244, 597, 1337
SduI GDGCHC 3 cut(s) 480, 1263, 1411
SfaNI GCATC 3 cut(s) 1075, 1166, 1477
SfcI CTRYAG 1 cut(s) 1452
SfoI GGCGCC 1 cut(s) 917
SinI GGWCC 2 cut(s) 565, 885
SphI GCATGC 1 cut(s) 947
Sse9I AATT 6 cut(s) 278, 607, 981, 1324, 1437, 1491
SspDI GGCGCC 1 cut(s) 915
SspI AATATT 1 cut(s) 1448
SspMI CTAG 2 cut(s) 920, 1510
SstI GAGCTC 2 cut(s) 480, 1263
StyD4I CCNGG 3 cut(s) 242, 595, 1335
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 3 cut(s) 856, 898, 1046
TaiI ACGT 2 cut(s) 458, 497
TaqI TCGA 5 cut(s) 204, 288, 312, 669, 692
TasI AATT 6 cut(s) 278, 607, 981, 1324, 1437, 1491
TatI WGTACW 1 cut(s) 555
TauI GCSGC 5 cut(s) 213, 227, 375, 403, 916
TfiI GAWTC 5 cut(s) 257, 377, 689, 1091, 1433
Tru1I TTAA 2 cut(s) 980, 1415
Tru9I TTAA 2 cut(s) 980, 1415
TscAI CASTG 2 cut(s) 575, 1051
TseI GCWGC 6 cut(s) 704, 707, 823, 910, 1154, 1305
TspDTI ATGAA 2 cut(s) 789, 1149
TspGWI ACGGA 3 cut(s) 495, 1046, 1288
TspRI CASTG 2 cut(s) 575, 1051
Van91I CCANNNNNTGG 1 cut(s) 574
VpaK11BI GGWCC 2 cut(s) 565, 885
XapI RAATTY 1 cut(s) 1324
XceI RCATGY 1 cut(s) 947
XspI CTAG 2 cut(s) 920, 1510
ZrmI AGTACT 1 cut(s) 557
Zsp2I ATGCAT 1 cut(s) 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.