Rorug02G0567200

TruB family pseudouridylate synthase (N terminal domain)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014138
Physical Location & Seq
Forward (+)
69166980 .. 69168464
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0567200.1

Sequence Viewer

Length: 1485 bp
ATGGCTGGTCATTTCGTGCGACGAGTCCTGTCGGCGGAGGCAGAGGAAACGGTGATTGATGGGGTTGAAACTAACCACGATCTGGGTCGGATTTATCACTTTTTGGTTGGAATGGTGCTTACGACAAAATCTGTGAATAAAGAGGCTCTGTTTAAGAAATTGAAGGAGCTTTGGCGTACAAAAGAGAAGTTTTCGTTTTGTGATATTGAGGGATCGGATCGGTTCTTGGTAGGGTTTAATTCAGATGATAGGAAGAGGGTGATGCATGGGTGTCCATGGACTTTTGATAATGCCCTAGTTGCTCTGGCGGAGACGGATGGAAGGATAGATGTAAGAGATGTTTGTCTGGATGAGCATGAAATCTGGATTCGGGTCAGAGGACTCCCTCCTCTATTCCTCAACAATGGGATGGGGGAGAGGATCGGGAACAGCGTTGGGAGGCATGTTTGGACTGACGGAGGTCGGAATGGGGATTTTCTTGGTGATTATCTTCGTATTAGGGTTTGTCTCAAGCTTTCTGCTCCAATTCCTCGGTGGGTGAATATTAGAGTGAACAATGGGTTAACTAAGAAGTTCGAATTGGAGTATGAGAGACTGCCTCATTTTTGCTTCTTCTGCGGTTTATTCTCCCATGTCAGCTCCTGCTGTCCAGAGAAGGAGAGTAATCCGCTGCTGGAGCCGAGCTATGGGCGATGGAAGACGACAATTCGTGATGTGTATAACATTAGAGTGAATGGTGAACTTAAAGGTAAGTCATTCGGATTGTTTAAGGAGTCTCCGGCATCAGGGTGGGCGTGTAGTGCTCCGGAACCCCAAATCTGTGGATCAGTACGCAGTTGGGAAGAAGCAGATTTGGAGGAATCAAATCCTCGACCTGTTAAGCGTTGCAGGGGGGCTCAGTTGGCAGAGAGTGTGGCGGCCAGAGGTGGTGGGGTGGCCGGGGATGAGCCTGGGCTGGATGCAACTACGTCGGAAAGATCGGAAAAGCAGGCTGCAGATTGTGGAAAGGAGAGGACTGATTTGTCGCGAGAATTAAAGGATGTTTTAATGACGGAAGTTATTGAGGATTTTGGGCGTGATAATAGGGATTTGGGGCTTTTCAAACAGAGCAAATCAAGCGGGAACTCTTCGTGTTGGAAAGGGAAGGAAAAACTTGATAAGGGTCCACTCTTTGAAGTCCCGGTGGTTGCTGGACCCAGTATATCCAAGGCCCATAAGCCCAAAGCTGTGAAGTCGCAAAGTGCAGGGGGTACTGATCAGAAGCATCAGAAGCTAGGGGAGAAGTTGAGCAGCATATCCAAGAAAACGATGCATATGTCTGTGGATCCTGCGGTAGTGAAGAGAATTGGCTTCCCAGTTGGAGGTAGTAGGCGCCCTAAATCGGGGAATGGGGTTGGTTCTCCAAGTAAAGCTCAGAAGTTTCGTACTGTGATCATGAAGAAAACCGTGATGGGTTCCGGTCCTAGGACCCATCACGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TruB_C_2 TruB_N

Protein Analysis

494

Amino Acids

54.85

Weight (kDa)

9.18

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 172 - 217 1.7e-08 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14460
fragaria_vesca FvH4_6g51780 FvH4_6g51780
malus_domestica MD17G1022100.v1.1
prunus_persica Prupe.3G294000_v2.0.a1
pyrus_communis pycom17g01920
rosa_chinensis RchiOBHm_Chr2g0173311
rosa_laevigata RLG00000022178
rosa_multiflora Rmu_sc0010961.1_g000005
rosa_roxburghii Rroxscaffold_2G00079060
rosa_rugosa Rorug02G0567200 Rorug02G0567300
rosa_samantha Rh2AG647600 Rh2BG658100 Rh2CG623500 Rh2DG671900
rosa_wichuraiana Rw2G053160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1021
AccB1I GGYRCC 1 cut(s) 1371
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 1027
AccIII TCCGGA 1 cut(s) 805
AciI CCGC 7 cut(s) 35, 308, 618, 668, 917, 1119, 1331
AclWI GGATC 6 cut(s) 220, 225, 428, 832, 1319, 1332
AcoI YGGCCR 2 cut(s) 918, 936
AcyI GRCGYC 1 cut(s) 1372
AfaI GTAC 4 cut(s) 178, 831, 1252, 1426
AfiI CCNNNNNNNGG 8 cut(s) 34, 82, 686, 785, 1361, 1381, 1382, 1464
AgsI TTSAA 4 cut(s) 68, 163, 1102, 1175
AjnI CCWGG 1 cut(s) 949
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AluBI AGCT 7 cut(s) 169, 514, 639, 684, 1226, 1273, 1412
AluI AGCT 7 cut(s) 169, 514, 639, 684, 1226, 1273, 1412
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 4 cut(s) 305, 512, 586, 780
AlwI GGATC 6 cut(s) 220, 225, 428, 832, 1319, 1332
AlwNI CAGNNNCTG 1 cut(s) 642
Aor13HI TCCGGA 1 cut(s) 805
AoxI GGCC 3 cut(s) 918, 936, 1209
ApeKI GCWGC 3 cut(s) 670, 992, 1290
AspA2I CCTAGG 1 cut(s) 1463
AspLEI GCGC 1 cut(s) 1374
AspS9I GGNCC 5 cut(s) 1163, 1193, 1210, 1460, 1467
AsuC2I CCSGG 2 cut(s) 940, 1181
AsuHPI GGTGA 5 cut(s) 64, 271, 494, 550, 749
AsuII TTCGAA 1 cut(s) 576
AvaII GGWCC 4 cut(s) 1163, 1193, 1460, 1467
AvrII CCTAGG 1 cut(s) 1463
BamHI GGATCC 1 cut(s) 1324
BanI GGYRCC 1 cut(s) 1371
BanII GRGCYC 1 cut(s) 898
BauI CACGAG 1 cut(s) 1475
BbsI GAAGAC 1 cut(s) 704
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 3 cut(s) 657, 979, 1302
BccI CCATC 6 cut(s) 53, 311, 403, 687, 1444, 1479
BcgI CGANNNNNNTGC 2 cut(s) 951, 985
BciT130I CCWGG 1 cut(s) 951
BclI TGATCA 2 cut(s) 1255, 1431
BcnI CCSGG 2 cut(s) 940, 1181
BcoDI GTCTC 4 cut(s) 305, 512, 586, 780
BfaI CTAG 3 cut(s) 296, 1274, 1464
BfmI CTRYAG 1 cut(s) 993
BfoI RGCGCY 1 cut(s) 1375
BisI GCNGC 4 cut(s) 671, 918, 993, 1291
BlnI CCTAGG 1 cut(s) 1463
BlsI GCNGC 4 cut(s) 672, 919, 994, 1292
Bme1390I CCNGG 3 cut(s) 940, 951, 1181
Bme18I GGWCC 4 cut(s) 1163, 1193, 1460, 1467
BmgT120I GGNCC 5 cut(s) 1163, 1193, 1210, 1460, 1467
BmiI GGNNCC 8 cut(s) 678, 810, 1164, 1195, 1326, 1373, 1456, 1469
BmrFI CCNGG 3 cut(s) 940, 951, 1181
BmrI ACTGGG 2 cut(s) 1191, 1349
BmsI GCATC 5 cut(s) 252, 791, 949, 1273, 1299
BmuI ACTGGG 2 cut(s) 1191, 1349
BoxI GACNNNNGTC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 704
BplI GAGNNNNNCTC 2 cut(s) 583, 615
BpmI CTGGAG 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 940, 1181
BsaHI GRCGYC 1 cut(s) 1372
BsaJI CCNNGG 6 cut(s) 275, 530, 939, 950, 1206, 1463
BsaWI WCCGGW 2 cut(s) 805, 1457
Bsc4I CCNNNNNNNGG 8 cut(s) 34, 82, 686, 785, 1361, 1381, 1382, 1464
Bse1I ACTGG 2 cut(s) 1197, 1355
BseAI TCCGGA 1 cut(s) 805
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 6 cut(s) 275, 530, 939, 950, 1206, 1463
BseGI GGATG 6 cut(s) 322, 355, 414, 949, 964, 1045
BseLI CCNNNNNNNGG 8 cut(s) 34, 82, 686, 785, 1361, 1381, 1382, 1464
BseMII CTCAG 2 cut(s) 911, 1427
BseNI ACTGG 2 cut(s) 1197, 1355
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 657, 979, 1302
BsgI GTGCAG 1 cut(s) 1263
Bsh1236I CGCG 1 cut(s) 1027
BshFI GGCC 3 cut(s) 920, 938, 1211
BshNI GGYRCC 1 cut(s) 1371
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 5 cut(s) 779, 806, 939, 1181, 1458
BslFI GGGAC 1 cut(s) 1163
BslI CCNNNNNNNGG 8 cut(s) 34, 82, 686, 785, 1361, 1381, 1382, 1464
BsmAI GTCTC 4 cut(s) 305, 512, 586, 780
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 1163
BsnI GGCC 3 cut(s) 920, 938, 1211
Bsp119I TTCGAA 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 805, 898
Bsp13I TCCGGA 1 cut(s) 805
Bsp143I GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
Bsp19I CCATGG 1 cut(s) 275
Bsp68I TCGCGA 1 cut(s) 1027
BspACI CCGC 7 cut(s) 35, 308, 618, 668, 917, 1119, 1331
BspANI GGCC 3 cut(s) 920, 938, 1211
BspCNI CTCAG 2 cut(s) 910, 1426
BspEI TCCGGA 1 cut(s) 805
BspFNI CGCG 1 cut(s) 1027
BspHI TCATGA 1 cut(s) 1434
BspLI GGNNCC 8 cut(s) 678, 810, 1164, 1195, 1326, 1373, 1456, 1469
BspMAI CTGCAG 1 cut(s) 997
BspPI GGATC 6 cut(s) 220, 225, 428, 832, 1319, 1332
BspT104I TTCGAA 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 1371
BsrI ACTGG 2 cut(s) 1197, 1355
BssECI CCNNGG 6 cut(s) 275, 530, 939, 950, 1206, 1463
BssMI GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
BssNI GRCGYC 1 cut(s) 1372
BssSI CACGAG 1 cut(s) 1475
BssT1I CCWWGG 3 cut(s) 275, 1206, 1463
Bst2BI CACGAG 1 cut(s) 1475
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 3 cut(s) 52, 1429, 1447
Bst6I CTCTTC 3 cut(s) 248, 1132, 1334
BstACI GRCGYC 1 cut(s) 1372
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 1 cut(s) 990
BstDEI CTNAG 3 cut(s) 567, 897, 1413
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 6 cut(s) 322, 355, 414, 949, 964, 1045
BstFNI CGCG 1 cut(s) 1027
BstH2I RGCGCY 1 cut(s) 1375
BstHHI GCGC 1 cut(s) 1374
BstKTI GATC 9 cut(s) 82, 215, 220, 423, 827, 980, 1258, 1327, 1434
BstMAI GTCTC 4 cut(s) 305, 512, 586, 780
BstMBI GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
BstMWI GCNNNNNNNGC 7 cut(s) 299, 615, 676, 800, 902, 1116, 1270
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 1 cut(s) 446
BstPAI GACNNNNGTC 1 cut(s) 459
BstSCI CCNGG 3 cut(s) 938, 949, 1179
BstSFI CTRYAG 1 cut(s) 993
BstUI CGCG 1 cut(s) 1027
BstV1I GCAGC 3 cut(s) 657, 979, 1302
BstV2I GAAGAC 1 cut(s) 704
BstX2I RGATCY 1 cut(s) 1324
BstXI CCANNNNNNTGG 1 cut(s) 821
BstYI RGATCY 1 cut(s) 1324
BsuRI GGCC 3 cut(s) 920, 938, 1211
BtgI CCRYGG 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 706
BtsCI GGATG 6 cut(s) 322, 355, 414, 949, 964, 1045
BtuMI TCGCGA 1 cut(s) 1027
Cac8I GCNNGC 1 cut(s) 990
CaiI CAGNNNCTG 1 cut(s) 642
CciI TCATGA 1 cut(s) 1434
CfoI GCGC 1 cut(s) 1374
Cfr13I GGNCC 5 cut(s) 1163, 1193, 1210, 1460, 1467
Csp6I GTAC 4 cut(s) 177, 830, 1251, 1425
CviAII CATG 6 cut(s) 266, 276, 356, 443, 632, 1435
CviQI GTAC 4 cut(s) 177, 830, 1251, 1425
DdeI CTNAG 3 cut(s) 567, 897, 1413
DinI GGCGCC 1 cut(s) 1373
DpnI GATC 9 cut(s) 81, 214, 219, 422, 826, 979, 1257, 1326, 1433
DpnII GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
DrdI GACNNNNNNGTC 1 cut(s) 1021
DseDI GACNNNNNNGTC 1 cut(s) 1021
EaeI YGGCCR 2 cut(s) 918, 936
Eam1104I CTCTTC 3 cut(s) 248, 1132, 1334
EarI CTCTTC 3 cut(s) 248, 1132, 1334
EciI GGCGGA 2 cut(s) 50, 323
Eco130I CCWWGG 3 cut(s) 275, 1206, 1463
Eco24I GRGCYC 1 cut(s) 898
Eco47I GGWCC 4 cut(s) 1163, 1193, 1460, 1467
EcoO109I RGGNCCY 1 cut(s) 1467
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 3 cut(s) 275, 1206, 1463
EcoT22I ATGCAT 2 cut(s) 267, 1314
EcoT38I GRGCYC 1 cut(s) 898
EgeI GGCGCC 1 cut(s) 1373
EheI GGCGCC 1 cut(s) 1373
ErhI CCWWGG 3 cut(s) 275, 1206, 1463
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 6 cut(s) 269, 279, 359, 446, 635, 1438
FaqI GGGAC 1 cut(s) 1163
FatI CATG 6 cut(s) 265, 275, 355, 442, 631, 1434
FauI CCCGC 1 cut(s) 1112
FauNDI CATATG 1 cut(s) 1314
FbaI TGATCA 2 cut(s) 1255, 1431
Fnu4HI GCNGC 4 cut(s) 671, 918, 993, 1291
FokI GGATG 6 cut(s) 329, 362, 421, 956, 971, 1052
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 4 cut(s) 671, 918, 993, 1291
FspBI CTAG 3 cut(s) 296, 1274, 1464
GlaI GCGC 1 cut(s) 1373
GluI GCNGC 4 cut(s) 671, 918, 993, 1291
GsuI CTGGAG 1 cut(s) 695
HaeII RGCGCY 1 cut(s) 1375
HaeIII GGCC 3 cut(s) 920, 938, 1211
HapII CCGG 5 cut(s) 779, 806, 939, 1181, 1458
HhaI GCGC 1 cut(s) 1374
Hin1I GRCGYC 1 cut(s) 1372
Hin1II CATG 6 cut(s) 269, 279, 359, 446, 635, 1438
Hin6I GCGC 1 cut(s) 1372
HinP1I GCGC 1 cut(s) 1372
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 5 cut(s) 24, 367, 381, 773, 860
HpaI GTTAAC 1 cut(s) 564
HpaII CCGG 5 cut(s) 779, 806, 939, 1181, 1458
HphI GGTGA 5 cut(s) 64, 271, 494, 550, 749
Hpy166II GTNNAC 4 cut(s) 553, 564, 740, 1166
Hpy188III TCNNGA 9 cut(s) 347, 364, 424, 650, 710, 806, 1026, 1435, 1475
Hpy8I GTNNAC 4 cut(s) 553, 564, 740, 1166
Hpy99I CGWCG 2 cut(s) 24, 973
HpyAV CCTTC 4 cut(s) 157, 315, 649, 1138
HpyCH4III ACNGT 3 cut(s) 52, 1429, 1447
HpyCH4IV ACGT 1 cut(s) 968
HpyCH4V TGCA 6 cut(s) 265, 888, 962, 995, 1244, 1312
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 615, 676, 800, 902, 1116, 1270
HpyF3I CTNAG 3 cut(s) 567, 897, 1413
HpySE526I ACGT 1 cut(s) 968
Hsp92I GRCGYC 1 cut(s) 1372
Hsp92II CATG 6 cut(s) 269, 279, 359, 446, 635, 1438
HspAI GCGC 1 cut(s) 1372
KasI GGCGCC 1 cut(s) 1371
Kpn2I TCCGGA 1 cut(s) 805
Ksp22I TGATCA 2 cut(s) 1255, 1431
KspAI GTTAAC 1 cut(s) 564
Kzo9I GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
LmnI GCTCC 5 cut(s) 166, 526, 644, 676, 808
Lsp1109I GCAGC 3 cut(s) 657, 979, 1302
LweI GCATC 5 cut(s) 252, 791, 949, 1273, 1299
MaeI CTAG 3 cut(s) 296, 1274, 1464
MaeII ACGT 1 cut(s) 968
MalI GATC 9 cut(s) 81, 214, 219, 422, 826, 979, 1257, 1326, 1433
MboI GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
MboII GAAGA 8 cut(s) 265, 482, 604, 709, 854, 1119, 1351, 1450
MflI RGATCY 1 cut(s) 1324
MhlI GDGCHC 2 cut(s) 805, 898
MluCI AATT 7 cut(s) 158, 238, 525, 578, 705, 1031, 1344
Mly113I GGCGCC 1 cut(s) 1372
MlyI GAGTC 3 cut(s) 33, 375, 782
MmeI TCCRAC 6 cut(s) 68, 88, 443, 951, 1115, 1339
Mph1103I ATGCAT 2 cut(s) 267, 1314
MroI TCCGGA 1 cut(s) 805
MseI TTAA 8 cut(s) 153, 237, 563, 744, 768, 879, 1034, 1046
MslI CAYNNNNRTG 2 cut(s) 728, 787
MspA1I CMGCKG 1 cut(s) 670
MspI CCGG 5 cut(s) 779, 806, 939, 1181, 1458
MspR9I CCNGG 3 cut(s) 940, 951, 1181
MvaI CCWGG 1 cut(s) 951
MvnI CGCG 1 cut(s) 1027
MwoI GCNNNNNNNGC 7 cut(s) 299, 615, 676, 800, 902, 1116, 1270
NarI GGCGCC 1 cut(s) 1372
NciI CCSGG 2 cut(s) 940, 1181
NcoI CCATGG 1 cut(s) 275
NdeI CATATG 1 cut(s) 1314
NdeII GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
NlaIII CATG 6 cut(s) 269, 279, 359, 446, 635, 1438
NlaIV GGNNCC 8 cut(s) 678, 810, 1164, 1195, 1326, 1373, 1456, 1469
NmeAIII GCCGAG 1 cut(s) 705
NruI TCGCGA 1 cut(s) 1027
NsiI ATGCAT 2 cut(s) 267, 1314
NspI RCATGY 1 cut(s) 446
NspV TTCGAA 1 cut(s) 576
PagI TCATGA 1 cut(s) 1434
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PfeI GAWTC 2 cut(s) 367, 860
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 4 cut(s) 672, 919, 994, 1292
PleI GAGTC 3 cut(s) 32, 375, 781
PluTI GGCGCC 1 cut(s) 1375
PpsI GAGTC 3 cut(s) 32, 375, 781
PpuMI RGGWCCY 1 cut(s) 1467
PshAI GACNNNNGTC 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 1467
Psp6I CCWGG 1 cut(s) 949
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 8 cut(s) 678, 810, 1164, 1195, 1326, 1373, 1456, 1469
PspPI GGNCC 5 cut(s) 1163, 1193, 1210, 1460, 1467
PspPPI RGGWCCY 1 cut(s) 1467
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 1 cut(s) 642
PsuI RGATCY 1 cut(s) 1324
RruI TCGCGA 1 cut(s) 1027
RsaI GTAC 4 cut(s) 178, 831, 1252, 1426
RsaNI GTAC 4 cut(s) 177, 830, 1251, 1425
RseI CAYNNNNRTG 2 cut(s) 728, 787
SaqAI TTAA 8 cut(s) 153, 237, 563, 744, 768, 879, 1034, 1046
SatI GCNGC 4 cut(s) 671, 918, 993, 1291
Sau3AI GATC 9 cut(s) 79, 212, 217, 420, 824, 977, 1255, 1324, 1431
Sau96I GGNCC 5 cut(s) 1163, 1193, 1210, 1460, 1467
SchI GAGTC 3 cut(s) 33, 375, 782
ScrFI CCNGG 3 cut(s) 940, 951, 1181
SduI GDGCHC 2 cut(s) 805, 898
SfaNI GCATC 5 cut(s) 252, 791, 949, 1273, 1299
SfcI CTRYAG 1 cut(s) 993
SfoI GGCGCC 1 cut(s) 1373
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 4 cut(s) 1163, 1193, 1460, 1467
SmiMI CAYNNNNRTG 2 cut(s) 728, 787
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 7 cut(s) 158, 238, 525, 578, 705, 1031, 1344
SsiI CCGC 7 cut(s) 35, 308, 618, 668, 917, 1119, 1331
SspDI GGCGCC 1 cut(s) 1371
SspI AATATT 1 cut(s) 544
SspMI CTAG 3 cut(s) 296, 1274, 1464
StyD4I CCNGG 3 cut(s) 938, 949, 1179
StyI CCWWGG 3 cut(s) 275, 1206, 1463
TaaI ACNGT 3 cut(s) 52, 1429, 1447
TaiI ACGT 1 cut(s) 971
TaqI TCGA 2 cut(s) 576, 871
TasI AATT 7 cut(s) 158, 238, 525, 578, 705, 1031, 1344
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 2 cut(s) 367, 860
Tru1I TTAA 8 cut(s) 153, 237, 563, 744, 768, 879, 1034, 1046
Tru9I TTAA 8 cut(s) 153, 237, 563, 744, 768, 879, 1034, 1046
TseI GCWGC 3 cut(s) 670, 992, 1290
TspDTI ATGAA 2 cut(s) 372, 1451
TspGWI ACGGA 3 cut(s) 329, 471, 1067
Van91I CCANNNNNTGG 1 cut(s) 82
VpaK11BI GGWCC 4 cut(s) 1163, 1193, 1460, 1467
XceI RCATGY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 531
XmaJI CCTAGG 1 cut(s) 1463
XspI CTAG 3 cut(s) 296, 1274, 1464
Zsp2I ATGCAT 2 cut(s) 267, 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.