MD17G1027100.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010411
Physical Location & Seq
Forward (+)
1934602 .. 1936035
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1027100.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGACAAGTTGCTATGTGATGAACTTGTCCAAGAAATCTTCCAAAGGCTGCCACCATCAACCTCCTCCTCCGTTTCTCTGGTCTCCAAGCGGTGGCTCCGCATCTACCGCACCTCCACAACTACCCTCTCTCTCCGCCTCACCACTCATCCTTCCACCCTCCCTTATCTCTCCTCCCTCCTCTCTCACTACCCTTTTCTTTCTTCCCTCTCTCTCCTCTTACCTCCCGACCCCACCACCTCCGCCACCGCAAAAACCACCGCTTTCGCGGACCACCTCCTCTTCCTAGTCTCCTCCTTCTGCCCCCAGCTTCTCAGCCTCAGGTTCTTGGCCGGCCCTGTCTCTCCTTCCTCCCTCGCTTCTCTCGCCTCATCATGTACCCAGCTCACCTCTCTGTATATCAATCTCTCCAGGCCTCTGTTCCTCATGTGGGTCCTCAAGTTTCCATCTTTGAAGGAACTGTCAGTCACGGTTTGCTCCGGGGACGGCGCTGATCATGCCGTTGACCCCAATTGGGAATATGGGTTTTCTGCAGAGGAGGATTCTGCTGCAGAATTGGGTTTGGATAGTCTCTGTTTATCTGGAATTGGAGCCGGCGATTGGGGTTTTGGCTGGCTATGGAGGAGCTGCAGAAAGCTGAGGAAATTGCAGTTGAAAAGCTGTGAAGGGATTGGGGATGGAGGGTCATTTTCGTCATTTGAAAGTTGTTTGCAGGGTGTTCAGGAGCTGGAGCTCCGGACTTGTAGGGCAATAATCGACGGTGTTCTGCTGAAAGTGGCGGAGAATTGTGATTCTCTGACTTCCCTTTTGGTTTATGATGGTGGCAGCAGTGAGGGCCTGCTCAGATTCTTTAGCCAAAGTCGTTGTAACTTGCGAAAAGTAGATTTTCGATTGCCCCTCGACCTTAATAACGATCATCTATTGGCTGTGGCGATGAATTTCAGAAGCCTTTCGAGTATTAAGCTTCAAAGTTGCTGTCTTGTCAGCGGTGAAGGCCTCAGAGCTCTTGCTATCGCCGCGAGTTCAGGCCTCGAAGAATTGGCACTGGTGAATTGTGATGTGGTTGAAAGAGAGCCGGGGTTGTTGGCCACATTAGGACAGAATTTGAGGAAATTGAGGAAGTTGGATTTGTCTTATAACGAAATGCTGGTGGATAAAGAGTTCATGTCGATGTTAGTTTCGTGTAATGATTTGGTTGATTTGAGGCTGAGAGGTTGTAGAAGGCTTACTACTGCAGCCATGGTTTCGATTTTTAAGAGCTGTAAGCGTTTGGAGAGTGTTGATATCATGCACTGTCGTGGGATTCAAGCCGAGGCGATTGAGTATTTGGTCCTCAACTCTCCTCAGCTGAGAGAGGTGCAGGTTGAGCAAAGCAAGATATCGGATGCAGCAAAGACTTGTGCCTCAAGTAAGTTCATTGAGGCTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

478

Amino Acids

52.36

Weight (kDa)

5.97

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 363 - 441 1.7e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1137
Acc36I ACCTGC 1 cut(s) 1351
AccB7I CCANNNNNTGG 1 cut(s) 93
AccII CGCG 2 cut(s) 269, 1019
AccIII TCCGGA 1 cut(s) 735
AcoI YGGCCR 2 cut(s) 330, 1086
AcsI RAATTY 2 cut(s) 937, 1102
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 4 cut(s) 93, 430, 514, 600
AgsI TTSAA 6 cut(s) 454, 655, 701, 968, 1067, 1307
AjnI CCWGG 1 cut(s) 410
AleI CACNNNNGTG 1 cut(s) 1296
AloI GAACNNNNNNTCC 2 cut(s) 1145, 1177
Alw21I GWGCWC 2 cut(s) 735, 1006
Alw26I GTCTC 4 cut(s) 88, 295, 346, 575
AlwNI CAGNNNCTG 1 cut(s) 727
Aor13HI TCCGGA 1 cut(s) 735
AoxI GGCC 7 cut(s) 330, 334, 413, 835, 994, 1027, 1086
ApeKI GCWGC 6 cut(s) 49, 548, 627, 825, 1235, 1388
ApoI RAATTY 2 cut(s) 937, 1102
ArsI GACNNNNNNTTYG 4 cut(s) 790, 822, 961, 993
Asp700I GAANNNNTTC 1 cut(s) 949
AspLEI GCGC 1 cut(s) 491
AspS9I GGNCC 5 cut(s) 271, 335, 433, 835, 1330
AsuC2I CCSGG 2 cut(s) 481, 1077
AsuHPI GGTGA 4 cut(s) 133, 379, 1001, 1060
AvaII GGWCC 3 cut(s) 271, 433, 1330
AxyI CCTNAGG 1 cut(s) 320
BalI TGGCCA 1 cut(s) 1088
BanII GRGCYC 2 cut(s) 735, 1006
BarI GAAGNNNNNNTAC 2 cut(s) 1213, 1245
Bbv12I GWGCWC 2 cut(s) 735, 1006
BbvCI CCTCAGC 2 cut(s) 638, 1344
BbvI GCAGC 6 cut(s) 36, 535, 614, 837, 1247, 1400
BccI CCATC 4 cut(s) 64, 454, 671, 812
BceAI ACGGC 2 cut(s) 485, 502
BciT130I CCWGG 1 cut(s) 412
BclI TGATCA 1 cut(s) 493
BcnI CCSGG 2 cut(s) 481, 1077
BcoDI GTCTC 4 cut(s) 88, 295, 346, 575
BfaI CTAG 1 cut(s) 287
BfmI CTRYAG 4 cut(s) 531, 549, 628, 1233
BfoI RGCGCY 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 1351
BisI GCNGC 7 cut(s) 50, 549, 628, 826, 1017, 1236, 1389
BlsI GCNGC 7 cut(s) 51, 550, 629, 827, 1018, 1237, 1390
Bme1390I CCNGG 3 cut(s) 412, 481, 1077
Bme18I GGWCC 3 cut(s) 271, 433, 1330
BmgT120I GGNCC 5 cut(s) 271, 335, 433, 835, 1330
BmiI GGNNCC 3 cut(s) 98, 434, 592
BmrFI CCNGG 3 cut(s) 412, 481, 1077
BmsI GCATC 3 cut(s) 111, 1375, 1417
BpmI CTGGAG 2 cut(s) 394, 749
Bpu10I CCTNAGC 2 cut(s) 638, 1344
BpuEI CTTGAG 2 cut(s) 422, 1390
BpuMI CCSGG 2 cut(s) 481, 1077
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 4 cut(s) 480, 1076, 1239, 1311
BsaWI WCCGGW 1 cut(s) 735
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 430, 514, 600
Bse118I RCCGGY 2 cut(s) 332, 593
Bse1I ACTGG 1 cut(s) 1050
Bse21I CCTNAGG 1 cut(s) 320
BseAI TCCGGA 1 cut(s) 735
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 4 cut(s) 480, 1076, 1239, 1311
BseGI GGATG 3 cut(s) 148, 682, 1390
BseLI CCNNNNNNNGG 4 cut(s) 93, 430, 514, 600
BseMII CTCAG 8 cut(s) 328, 334, 629, 856, 1012, 1199, 1340, 1358
BseNI ACTGG 1 cut(s) 1050
BseXI GCAGC 6 cut(s) 36, 535, 614, 837, 1247, 1400
BseYI CCCAGC 2 cut(s) 306, 381
BsgI GTGCAG 1 cut(s) 1379
Bsh1236I CGCG 2 cut(s) 269, 1019
BshFI GGCC 7 cut(s) 332, 336, 415, 837, 996, 1029, 1088
BsiHKAI GWGCWC 2 cut(s) 735, 1006
BsiSI CCGG 5 cut(s) 333, 480, 594, 736, 1076
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 4 cut(s) 93, 430, 514, 600
BsmAI GTCTC 4 cut(s) 88, 295, 346, 575
BsmFI GGGAC 1 cut(s) 497
BsnI GGCC 7 cut(s) 332, 336, 415, 837, 996, 1029, 1088
Bso31I GGTCTC 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 735, 1006
Bsp13I TCCGGA 1 cut(s) 735
Bsp143I GATC 2 cut(s) 493, 913
Bsp19I CCATGG 1 cut(s) 1239
BspANI GGCC 7 cut(s) 332, 336, 415, 837, 996, 1029, 1088
BspCNI CTCAG 8 cut(s) 327, 333, 630, 855, 1011, 1200, 1341, 1357
BspEI TCCGGA 1 cut(s) 735
BspFNI CGCG 2 cut(s) 269, 1019
BspLI GGNNCC 3 cut(s) 98, 434, 592
BspMAI CTGCAG 4 cut(s) 535, 553, 632, 1237
BspMI ACCTGC 1 cut(s) 1351
BspTNI GGTCTC 1 cut(s) 88
BsrFI RCCGGY 2 cut(s) 332, 593
BsrI ACTGG 1 cut(s) 1050
BssAI RCCGGY 2 cut(s) 332, 593
BssECI CCNNGG 4 cut(s) 480, 1076, 1239, 1311
BssMI GATC 2 cut(s) 493, 913
BssT1I CCWWGG 1 cut(s) 1239
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 4 cut(s) 462, 472, 761, 1295
Bst6I CTCTTC 1 cut(s) 287
BstC8I GCNNGC 4 cut(s) 334, 595, 614, 839
BstDEI CTNAG 8 cut(s) 314, 320, 638, 842, 998, 1208, 1344, 1349
BstDSI CCRYGG 1 cut(s) 1239
BstF5I GGATG 3 cut(s) 148, 682, 1390
BstFNI CGCG 2 cut(s) 269, 1019
BstH2I RGCGCY 1 cut(s) 492
BstHHI GCGC 1 cut(s) 491
BstKTI GATC 2 cut(s) 496, 916
BstMAI GTCTC 4 cut(s) 88, 295, 346, 575
BstMBI GATC 2 cut(s) 493, 913
BstMWI GCNNNNNNNGC 7 cut(s) 108, 365, 497, 834, 993, 1016, 1366
BstNI CCWGG 1 cut(s) 412
BstSCI CCNGG 3 cut(s) 410, 479, 1075
BstSFI CTRYAG 4 cut(s) 531, 549, 628, 1233
BstUI CGCG 2 cut(s) 269, 1019
BstV1I GCAGC 6 cut(s) 36, 535, 614, 837, 1247, 1400
Bsu36I CCTNAGG 1 cut(s) 320
BsuRI GGCC 7 cut(s) 332, 336, 415, 837, 996, 1029, 1088
BtgI CCRYGG 1 cut(s) 1239
BtgZI GCGATG 1 cut(s) 947
BtsCI GGATG 3 cut(s) 148, 682, 1390
BtsI GCAGTG 1 cut(s) 835
BtsIMutI CAGTG 3 cut(s) 835, 1043, 1291
BveI ACCTGC 1 cut(s) 1351
Cac8I GCNNGC 4 cut(s) 334, 595, 614, 839
CaiI CAGNNNCTG 1 cut(s) 727
CfoI GCGC 1 cut(s) 491
Cfr10I RCCGGY 2 cut(s) 332, 593
Cfr13I GGNCC 5 cut(s) 271, 335, 433, 835, 1330
Csp6I GTAC 1 cut(s) 378
CviAII CATG 6 cut(s) 375, 427, 497, 1165, 1240, 1288
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 8 cut(s) 314, 320, 638, 842, 998, 1208, 1344, 1349
DpnI GATC 2 cut(s) 495, 915
DpnII GATC 2 cut(s) 493, 913
EaeI YGGCCR 2 cut(s) 330, 1086
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 3 cut(s) 125, 232, 794
Ecl136II GAGCTC 2 cut(s) 733, 1004
Eco130I CCWWGG 1 cut(s) 1239
Eco147I AGGCCT 3 cut(s) 415, 996, 1029
Eco24I GRGCYC 2 cut(s) 735, 1006
Eco31I GGTCTC 1 cut(s) 88
Eco32I GATATC 2 cut(s) 1285, 1380
Eco47I GGWCC 3 cut(s) 271, 433, 1330
Eco53kI GAGCTC 2 cut(s) 733, 1004
Eco81I CCTNAGG 1 cut(s) 320
EcoICRI GAGCTC 2 cut(s) 733, 1004
EcoO109I RGGNCCY 2 cut(s) 433, 835
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 2 cut(s) 1285, 1380
EcoT14I CCWWGG 1 cut(s) 1239
EcoT38I GRGCYC 2 cut(s) 735, 1006
ErhI CCWWGG 1 cut(s) 1239
FaeI CATG 6 cut(s) 378, 430, 500, 1168, 1243, 1291
FaqI GGGAC 1 cut(s) 497
FatI CATG 6 cut(s) 374, 426, 496, 1164, 1239, 1287
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 7 cut(s) 50, 549, 628, 826, 1017, 1236, 1389
FokI GGATG 3 cut(s) 135, 689, 1397
FriOI GRGCYC 2 cut(s) 735, 1006
FseI GGCCGGCC 1 cut(s) 336
Fsp4HI GCNGC 7 cut(s) 50, 549, 628, 826, 1017, 1236, 1389
FspBI CTAG 1 cut(s) 287
GlaI GCGC 1 cut(s) 490
GluI GCNGC 7 cut(s) 50, 549, 628, 826, 1017, 1236, 1389
GsaI CCCAGC 2 cut(s) 310, 385
GsuI CTGGAG 2 cut(s) 394, 749
HaeII RGCGCY 1 cut(s) 492
HaeIII GGCC 7 cut(s) 332, 336, 415, 837, 996, 1029, 1088
HapII CCGG 5 cut(s) 333, 480, 594, 736, 1076
HhaI GCGC 1 cut(s) 491
Hin1II CATG 6 cut(s) 378, 430, 500, 1168, 1243, 1291
Hin6I GCGC 1 cut(s) 489
HinP1I GCGC 1 cut(s) 489
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 962
HinfI GANTC 4 cut(s) 542, 791, 846, 1303
HpaII CCGG 5 cut(s) 333, 480, 594, 736, 1076
HphI GGTGA 4 cut(s) 133, 379, 1001, 1060
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 5 cut(s) 798, 845, 944, 1001, 1384
Hpy188III TCNNGA 4 cut(s) 227, 582, 722, 736
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 7 cut(s) 162, 307, 357, 448, 659, 986, 1215
HpyCH4III ACNGT 4 cut(s) 462, 472, 761, 1295
HpyCH4V TGCA 9 cut(s) 533, 551, 630, 649, 712, 1235, 1291, 1360, 1388
HpyF10VI GCNNNNNNNGC 7 cut(s) 108, 365, 497, 834, 993, 1016, 1366
HpyF3I CTNAG 8 cut(s) 314, 320, 638, 842, 998, 1208, 1344, 1349
Hsp92II CATG 6 cut(s) 378, 430, 500, 1168, 1243, 1291
HspAI GCGC 1 cut(s) 489
Kpn2I TCCGGA 1 cut(s) 735
KroI GCCGGC 2 cut(s) 332, 593
KroNI GCCGGC 2 cut(s) 334, 595
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 2 cut(s) 493, 913
LmnI GCTCC 7 cut(s) 102, 482, 590, 624, 724, 730, 738
Lsp1109I GCAGC 6 cut(s) 36, 535, 614, 837, 1247, 1400
LweI GCATC 3 cut(s) 111, 1375, 1417
MaeI CTAG 1 cut(s) 287
MaeIII GTNAC 2 cut(s) 466, 866
MalI GATC 2 cut(s) 495, 915
MboI GATC 2 cut(s) 493, 913
MboII GAAGA 4 cut(s) 31, 195, 274, 1046
MfeI CAATTG 1 cut(s) 511
MhlI GDGCHC 2 cut(s) 735, 1006
MlsI TGGCCA 1 cut(s) 1088
MluNI TGGCCA 1 cut(s) 1088
MmeI TCCRAC 1 cut(s) 1104
Mox20I TGGCCA 1 cut(s) 1088
MroI TCCGGA 1 cut(s) 735
MroNI GCCGGC 2 cut(s) 332, 593
MroXI GAANNNNTTC 1 cut(s) 949
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 3 cut(s) 906, 960, 1254
MslI CAYNNNNRTG 2 cut(s) 1169, 1296
Msp20I TGGCCA 1 cut(s) 1088
MspA1I CMGCKG 2 cut(s) 987, 1348
MspI CCGG 5 cut(s) 333, 480, 594, 736, 1076
MspR9I CCNGG 3 cut(s) 412, 481, 1077
MunI CAATTG 1 cut(s) 511
MvaI CCWGG 1 cut(s) 412
MvnI CGCG 2 cut(s) 269, 1019
MwoI GCNNNNNNNGC 7 cut(s) 108, 365, 497, 834, 993, 1016, 1366
NaeI GCCGGC 2 cut(s) 334, 595
NciI CCSGG 2 cut(s) 481, 1077
NcoI CCATGG 1 cut(s) 1239
NdeII GATC 2 cut(s) 493, 913
NgoMIV GCCGGC 2 cut(s) 332, 593
NlaIII CATG 6 cut(s) 378, 430, 500, 1168, 1243, 1291
NlaIV GGNNCC 3 cut(s) 98, 434, 592
NmeAIII GCCGAG 1 cut(s) 1336
NmuCI GTSAC 1 cut(s) 466
OliI CACNNNNGTG 1 cut(s) 1296
PceI AGGCCT 3 cut(s) 415, 996, 1029
PdiI GCCGGC 2 cut(s) 334, 595
PdmI GAANNNNTTC 1 cut(s) 949
PfeI GAWTC 4 cut(s) 542, 791, 846, 1303
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 7 cut(s) 51, 550, 629, 827, 1018, 1237, 1390
PpuMI RGGWCCY 1 cut(s) 433
PsiI TTATAA 1 cut(s) 1137
Psp124BI GAGCTC 2 cut(s) 735, 1006
Psp5II RGGWCCY 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 410
PspFI CCCAGC 2 cut(s) 306, 381
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 3 cut(s) 98, 434, 592
PspPI GGNCC 5 cut(s) 271, 335, 433, 835, 1330
PspPPI RGGWCCY 1 cut(s) 433
PstI CTGCAG 4 cut(s) 535, 553, 632, 1237
PstNI CAGNNNCTG 1 cut(s) 727
PvuII CAGCTG 1 cut(s) 1348
RigI GGCCGGCC 1 cut(s) 336
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 2 cut(s) 1169, 1296
SacI GAGCTC 2 cut(s) 735, 1006
SaqAI TTAA 3 cut(s) 906, 960, 1254
SatI GCNGC 7 cut(s) 50, 549, 628, 826, 1017, 1236, 1389
Sau3AI GATC 2 cut(s) 493, 913
Sau96I GGNCC 5 cut(s) 271, 335, 433, 835, 1330
ScrFI CCNGG 3 cut(s) 412, 481, 1077
SduI GDGCHC 2 cut(s) 735, 1006
SfaNI GCATC 3 cut(s) 111, 1375, 1417
SfcI CTRYAG 4 cut(s) 531, 549, 628, 1233
SinI GGWCC 3 cut(s) 271, 433, 1330
SmiMI CAYNNNNRTG 2 cut(s) 1169, 1296
SmlI CTYRAG 2 cut(s) 437, 1405
SmoI CTYRAG 2 cut(s) 437, 1405
SseBI AGGCCT 3 cut(s) 415, 996, 1029
SspMI CTAG 1 cut(s) 287
SstI GAGCTC 2 cut(s) 735, 1006
StuI AGGCCT 3 cut(s) 415, 996, 1029
StyD4I CCNGG 3 cut(s) 410, 479, 1075
StyI CCWWGG 1 cut(s) 1239
TaaI ACNGT 4 cut(s) 462, 472, 761, 1295
TaqI TCGA 7 cut(s) 756, 889, 900, 953, 1032, 1169, 1247
TauI GCSGC 1 cut(s) 1019
TfiI GAWTC 4 cut(s) 542, 791, 846, 1303
Tru1I TTAA 3 cut(s) 906, 960, 1254
Tru9I TTAA 3 cut(s) 906, 960, 1254
TscAI CASTG 3 cut(s) 835, 1050, 1298
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 6 cut(s) 49, 548, 627, 825, 1235, 1388
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 4 cut(s) 36, 950, 1153, 1405
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 3 cut(s) 835, 1050, 1298
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 3 cut(s) 271, 433, 1330
XapI RAATTY 2 cut(s) 937, 1102
XmnI GAANNNNTTC 1 cut(s) 949
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.