MD17G1079300.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018905
Physical Location & Seq
Forward (+)
6543843 .. 6544901
1059 bp
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UTR
Exon/CDS
Intron
MD17G1079300.v1.1.491

Sequence Viewer

Length: 387 bp
ATGGCACCGGTGTACCATGGTGTGTCACATGTAGGTGGTGATATGTTTGAGGGCAATATTCCAAATGCTGATGCAATTTTTATGAAGTGGGTTATGCACGATTGGAGTGACAGTGATTGCATCAAGATTTTGAAGAATTGTCGAAAAGTAATACCAGAGAAAAGTGGGAAGATCATTATTGTTGATATAGTTCTAGAGCCAAATGGTGAAGGGATTTTGGATGACACAGGATTGGTCTTTGATTTGTTGATGATTGCACATGCGTCAGGTGGAAGGGAGAGGACTGAGAGTGAATGGAAGAAGATATTGGAAGGAGGAGGTTTCCCTCGCTACAAAGTCATCAAAATTCCAGCTATAGCATCCATTGTTGAGGCCTACCCTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

129

Amino Acids

14.15

Weight (kDa)

5.51

Isoelectric Point (pI)

22.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 6 - 108 1.8e-31 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018905)

Species Orthologous Gene IDs
malus_domestica MD17G1079300.v1.1 MD17G1079400.v1.1
pyrus_communis pycom09g01480
rosa_chinensis RchiOBHm_Chr2g0162441
rosa_multiflora Rmu_co8443219.1_g000001
rosa_roxburghii Rroxscaffold_2G00087920
rosa_samantha Rh2AG405000 Rh2AG563600 Rh2BG576400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 345
AfaI GTAC 1 cut(s) 14
AflIII ACRYGT 1 cut(s) 28
AgeI ACCGGT 1 cut(s) 7
AgsI TTSAA 1 cut(s) 133
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 1 cut(s) 353
AluI AGCT 1 cut(s) 353
AoxI GGCC 1 cut(s) 372
ApoI RAATTY 1 cut(s) 345
AsiGI ACCGGT 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 50, 218
BaeI ACNNNNGTAYC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 4
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 354
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 3 cut(s) 61, 129, 368
BsaJI CCNNGG 1 cut(s) 16
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bse118I RCCGGY 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 16
BseGI GGATG 2 cut(s) 226, 359
BseMII CTCAG 1 cut(s) 276
BseRI GAGGAG 1 cut(s) 330
BshFI GGCC 1 cut(s) 374
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 7
BsiSI CCGG 1 cut(s) 8
BsnI GGCC 1 cut(s) 374
Bsp143I GATC 1 cut(s) 171
Bsp19I CCATGG 1 cut(s) 16
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 16
BssMI GATC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 113
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 16
BstF5I GGATG 2 cut(s) 226, 359
BstKTI GATC 1 cut(s) 174
BstMBI GATC 1 cut(s) 171
BstNSI RCATGY 2 cut(s) 32, 263
BstSFI CTRYAG 1 cut(s) 354
BsuRI GGCC 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 16
BtsCI GGATG 2 cut(s) 226, 359
BtsIMutI CAGTG 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 7
CseI GACGC 1 cut(s) 252
Csp6I GTAC 1 cut(s) 13
CspAI ACCGGT 1 cut(s) 7
CviAII CATG 3 cut(s) 17, 29, 260
CviJI RGCY 3 cut(s) 199, 353, 374
CviKI_1 RGCY 3 cut(s) 199, 353, 374
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 1 cut(s) 285
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 16
Eco147I AGGCCT 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 3 cut(s) 20, 32, 263
FaiI YATR 9 cut(s) 18, 30, 44, 83, 95, 188, 261, 356, 383
FatI CATG 3 cut(s) 16, 28, 259
FokI GGATG 2 cut(s) 233, 346
FspBI CTAG 1 cut(s) 194
HaeIII GGCC 1 cut(s) 374
HapII CCGG 1 cut(s) 8
HgaI GACGC 1 cut(s) 252
Hin1II CATG 3 cut(s) 20, 32, 263
HpaII CCGG 1 cut(s) 8
HphI GGTGA 2 cut(s) 50, 218
Hpy166II GTNNAC 1 cut(s) 13
Hpy188III TCNNGA 2 cut(s) 124, 194
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 203, 267, 305
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4V TGCA 4 cut(s) 74, 97, 120, 257
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 3 cut(s) 20, 32, 263
Kzo9I GATC 1 cut(s) 171
LpnPI CCDG 5 cut(s) 21, 168, 213, 252, 363
LweI GCATC 3 cut(s) 61, 129, 368
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 24, 107
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 4 cut(s) 145, 181, 310, 313
MluCI AATT 3 cut(s) 75, 136, 345
MnlI CCTC 6 cut(s) 43, 273, 308, 311, 336, 364
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 1 cut(s) 8
NcoI CCATGG 1 cut(s) 16
NdeII GATC 1 cut(s) 171
NlaIII CATG 3 cut(s) 20, 32, 263
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 24, 107
NspI RCATGY 2 cut(s) 32, 263
PceI AGGCCT 1 cut(s) 374
PciI ACATGT 1 cut(s) 28
PinAI ACCGGT 1 cut(s) 7
PscI ACATGT 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 6
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
RseI CAYNNNNRTG 1 cut(s) 33
Sau3AI GATC 1 cut(s) 171
SetI ASST 4 cut(s) 37, 271, 322, 355
SfaNI GCATC 3 cut(s) 61, 129, 368
SfcI CTRYAG 1 cut(s) 354
SgrAI CRCCGGYG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 3 cut(s) 75, 136, 345
SseBI AGGCCT 1 cut(s) 374
SspI AATATT 1 cut(s) 58
SspMI CTAG 1 cut(s) 194
StuI AGGCCT 1 cut(s) 374
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 142
TasI AATT 3 cut(s) 75, 136, 345
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 2 cut(s) 24, 107
Tsp45I GTSAC 2 cut(s) 24, 107
TspDTI ATGAA 1 cut(s) 98
TspRI CASTG 1 cut(s) 118
XapI RAATTY 1 cut(s) 345
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 2 cut(s) 32, 263
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.