Rroxscaffold_2G00087920
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018905
Physical Location & Seq
Forward (+)
9958946 .. 9969472
10527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087920.1

Sequence Viewer

Length: 741 bp
ATGACGTGGCAACACCGATGGATGTTGGTGGTGGGATTGGACGCTCGGGTGGCGGAGATTGTGAAGTTGTATCCCAGCATTAAGGGCATCAACTTTGATCTACCACATCTAGTTGCAACAGCGCCGGTGTACCATAGGGTGTCACATGTTGGAGGTGACATGTTTGATGAGGGCAATATTCCAAACGCTGATGCAATTTTCATGAAGACCGGCCAGAAACATGCCCCTAAAGCTGGACTCATAGGTTGTTTTCCAGCATCGACGCCAACCCCCGACCGTGCATCAAATCACAGCACGAATTGCCTTGCCGTCCACGACATCGATCACCTCTTGCGCCTCATGAGATTTCTCGTCCACAAAAATGTCTTCGAGGCAACCACCGATCCCAAAAGCGGAGATACCCTCTACGGCCTAACTTATTCATCCAAATATATGGCAACAATGCCAAAACTGAAAGAAGAAAAAGAGGAAGCCATGCTGCTACAAGGCCAAGCTAATATTTTTCACTACACGTACAACTTTGTCAAAAGCATGGCCTTGAAATGCGCTGTGAAACTTGGCATTGCAGACATCATAAACTCTCAAGGCCAACCCCCGACCGTGCATCAAATCACCAGCATGAATTGCCTCGTCATCCACGGACATCGATCACCTCTTGCGCCTCATGAGATTTCTCGTCCACAAAAATGTCTTCGAGGCAACCACCGATCCCCAAAAGCGGAGATACCCTCTATGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

246

Amino Acids

27.36

Weight (kDa)

9.06

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 12 - 69 6.1e-14 O-methyltransferase domain
Dimerisation PF08100 169 - 205 9.9e-07 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018905)

Species Orthologous Gene IDs
malus_domestica MD17G1079300.v1.1 MD17G1079400.v1.1
pyrus_communis pycom09g01480
rosa_chinensis RchiOBHm_Chr2g0162441
rosa_multiflora Rmu_co8443219.1_g000001
rosa_roxburghii Rroxscaffold_2G00087920
rosa_samantha Rh2AG405000 Rh2AG563600 Rh2BG576400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 53, 393, 719
AclWI GGATC 2 cut(s) 377, 702
AcoI YGGCCR 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 2 cut(s) 131, 515
AfiI CCNNNNNNNGG 3 cut(s) 233, 392, 718
AflIII ACRYGT 3 cut(s) 145, 159, 510
AgsI TTSAA 1 cut(s) 541
AjiI CACGTC 1 cut(s) 6
AluBI AGCT 2 cut(s) 233, 494
AluI AGCT 2 cut(s) 233, 494
AlwI GGATC 2 cut(s) 377, 702
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 5 cut(s) 211, 409, 487, 534, 586
ApeKI GCWGC 1 cut(s) 478
AspLEI GCGC 4 cut(s) 124, 336, 548, 661
AsuHPI GGTGA 4 cut(s) 167, 317, 604, 642
AvaI CYCGRG 1 cut(s) 45
BbsI GAAGAC 3 cut(s) 212, 358, 683
BbvI GCAGC 1 cut(s) 465
BccI CCATC 1 cut(s) 12
BceAI ACGGC 2 cut(s) 293, 424
BciVI GTATCC 1 cut(s) 81
BfaI CTAG 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 125
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 1 cut(s) 479
BlsI GCNGC 1 cut(s) 480
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 6
BmsI GCATC 5 cut(s) 96, 181, 266, 290, 613
BpiI GAAGAC 3 cut(s) 212, 358, 683
BplI GAGNNNNNCTC 3 cut(s) 387, 419, 713
BpuEI CTTGAG 1 cut(s) 567
Bsa29I ATCGAT 2 cut(s) 321, 646
BsaAI YACGTR 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 637
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 392, 718
Bse118I RCCGGY 2 cut(s) 124, 209
Bse3DI GCAATG 1 cut(s) 561
BseCI ATCGAT 2 cut(s) 321, 646
BseDI CCNNGG 1 cut(s) 637
BseGI GGATG 3 cut(s) 27, 422, 633
BseLI CCNNNNNNNGG 3 cut(s) 233, 392, 718
BseMI GCAATG 1 cut(s) 561
BseXI GCAGC 1 cut(s) 465
BseYI CCCAGC 1 cut(s) 74
Bsh1285I CGRYCG 2 cut(s) 277, 600
BshFI GGCC 5 cut(s) 213, 411, 489, 536, 588
BshVI ATCGAT 2 cut(s) 321, 646
BsiEI CGRYCG 2 cut(s) 277, 600
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 2 cut(s) 125, 210
BslI CCNNNNNNNGG 3 cut(s) 233, 392, 718
BsnI GGCC 5 cut(s) 213, 411, 489, 536, 588
BsoBI CYCGRG 1 cut(s) 45
Bsp143I GATC 5 cut(s) 97, 322, 382, 647, 707
BspACI CCGC 3 cut(s) 53, 393, 719
BspANI GGCC 5 cut(s) 213, 411, 489, 536, 588
BspDI ATCGAT 2 cut(s) 321, 646
BspHI TCATGA 3 cut(s) 201, 339, 664
BspPI GGATC 2 cut(s) 377, 702
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 2 cut(s) 124, 209
BssAI RCCGGY 2 cut(s) 124, 209
BssECI CCNNGG 1 cut(s) 637
BssMI GATC 5 cut(s) 97, 322, 382, 647, 707
BssNI GRCGYC 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 278, 601
BstACI GRCGYC 1 cut(s) 263
BstAPI GCANNNNNTGC 2 cut(s) 300, 624
BstBAI YACGTR 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 637
BstF5I GGATG 3 cut(s) 27, 422, 633
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 4 cut(s) 124, 336, 548, 661
BstKTI GATC 5 cut(s) 100, 325, 385, 650, 710
BstMBI GATC 5 cut(s) 97, 322, 382, 647, 707
BstMCI CGRYCG 2 cut(s) 277, 600
BstMWI GCNNNNNNNGC 5 cut(s) 50, 84, 230, 300, 624
BstNSI RCATGY 3 cut(s) 149, 163, 224
BstV1I GCAGC 1 cut(s) 465
BstV2I GAAGAC 3 cut(s) 212, 358, 683
BstXI CCANNNNNNTGG 1 cut(s) 433
Bsu15I ATCGAT 2 cut(s) 321, 646
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 5 cut(s) 213, 411, 489, 536, 588
BsuTUI ATCGAT 2 cut(s) 321, 646
BtgI CCRYGG 1 cut(s) 637
BtrI CACGTC 1 cut(s) 6
BtsCI GGATG 3 cut(s) 27, 422, 633
CciI TCATGA 3 cut(s) 201, 339, 664
CfoI GCGC 4 cut(s) 124, 336, 548, 661
Cfr10I RCCGGY 2 cut(s) 124, 209
ClaI ATCGAT 2 cut(s) 321, 646
CseI GACGC 2 cut(s) 50, 271
Csp6I GTAC 2 cut(s) 130, 514
CviAII CATG 9 cut(s) 146, 160, 202, 221, 340, 475, 532, 619, 665
CviJI RGCY 8 cut(s) 213, 233, 411, 473, 489, 494, 536, 588
CviKI_1 RGCY 8 cut(s) 213, 233, 411, 473, 489, 494, 536, 588
CviQI GTAC 2 cut(s) 130, 514
DpnI GATC 5 cut(s) 99, 324, 384, 649, 709
DpnII GATC 5 cut(s) 97, 322, 382, 647, 707
EaeI YGGCCR 1 cut(s) 211
EciI GGCGGA 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 45
FaeI CATG 9 cut(s) 149, 163, 205, 224, 343, 478, 535, 622, 668
FatI CATG 9 cut(s) 145, 159, 201, 220, 339, 474, 531, 618, 664
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 3 cut(s) 34, 409, 620
Fsp4HI GCNGC 1 cut(s) 479
FspBI CTAG 1 cut(s) 110
GlaI GCGC 4 cut(s) 123, 335, 547, 660
GluI GCNGC 1 cut(s) 479
GsaI CCCAGC 1 cut(s) 78
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 5 cut(s) 213, 411, 489, 536, 588
HapII CCGG 2 cut(s) 125, 210
HgaI GACGC 2 cut(s) 50, 271
HhaI GCGC 4 cut(s) 124, 336, 548, 661
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 9 cut(s) 149, 163, 205, 224, 343, 478, 535, 622, 668
Hin6I GCGC 4 cut(s) 122, 334, 546, 659
HinP1I GCGC 4 cut(s) 122, 334, 546, 659
HinfI GANTC 1 cut(s) 237
HpaII CCGG 2 cut(s) 125, 210
HphI GGTGA 4 cut(s) 167, 317, 604, 642
Hpy166II GTNNAC 4 cut(s) 130, 313, 355, 680
Hpy188III TCNNGA 3 cut(s) 202, 340, 665
Hpy8I GTNNAC 4 cut(s) 130, 313, 355, 680
Hpy99I CGWCG 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 278, 601
HpyCH4IV ACGT 2 cut(s) 5, 512
HpyCH4V TGCA 5 cut(s) 116, 194, 281, 566, 604
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 84, 230, 300, 624
HpySE526I ACGT 2 cut(s) 5, 512
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 9 cut(s) 149, 163, 205, 224, 343, 478, 535, 622, 668
HspAI GCGC 4 cut(s) 122, 334, 546, 659
Kzo9I GATC 5 cut(s) 97, 322, 382, 647, 707
LpnPI CCDG 7 cut(s) 88, 138, 219, 223, 227, 267, 628
Lsp1109I GCAGC 1 cut(s) 465
LweI GCATC 5 cut(s) 96, 181, 266, 290, 613
MaeI CTAG 1 cut(s) 110
MaeII ACGT 2 cut(s) 5, 512
MaeIII GTNAC 2 cut(s) 141, 155
MalI GATC 5 cut(s) 99, 324, 384, 649, 709
MboI GATC 5 cut(s) 97, 322, 382, 647, 707
MboII GAAGA 4 cut(s) 217, 358, 470, 683
MluCI AATT 3 cut(s) 195, 298, 622
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 130
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 3 cut(s) 360, 617, 685
MspI CCGG 2 cut(s) 125, 210
MwoI GCNNNNNNNGC 5 cut(s) 50, 84, 230, 300, 624
NdeII GATC 5 cut(s) 97, 322, 382, 647, 707
NlaIII CATG 9 cut(s) 149, 163, 205, 224, 343, 478, 535, 622, 668
NmuCI GTSAC 2 cut(s) 141, 155
NspI RCATGY 3 cut(s) 149, 163, 224
PagI TCATGA 3 cut(s) 201, 339, 664
PciI ACATGT 2 cut(s) 145, 159
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
Ppu21I YACGTR 1 cut(s) 513
PscI ACATGT 2 cut(s) 145, 159
PspFI CCCAGC 1 cut(s) 74
RsaI GTAC 2 cut(s) 131, 515
RsaNI GTAC 2 cut(s) 130, 514
RseI CAYNNNNRTG 3 cut(s) 360, 617, 685
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 5 cut(s) 97, 322, 382, 647, 707
SchI GAGTC 1 cut(s) 231
SetI ASST 9 cut(s) 8, 157, 235, 247, 330, 496, 515, 655, 740
SfaNI GCATC 5 cut(s) 96, 181, 266, 290, 613
SgrAI CRCCGGYG 1 cut(s) 124
SmiMI CAYNNNNRTG 3 cut(s) 360, 617, 685
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 3 cut(s) 195, 298, 622
SsiI CCGC 3 cut(s) 53, 393, 719
SspI AATATT 2 cut(s) 178, 499
SspMI CTAG 1 cut(s) 110
TaaI ACNGT 2 cut(s) 278, 601
TaiI ACGT 2 cut(s) 8, 515
TaqI TCGA 5 cut(s) 260, 321, 369, 646, 694
TasI AATT 3 cut(s) 195, 298, 622
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseFI GTSAC 2 cut(s) 141, 155
TseI GCWGC 1 cut(s) 478
Tsp45I GTSAC 2 cut(s) 141, 155
TspDTI ATGAA 4 cut(s) 190, 218, 411, 635
TspGWI ACGGA 1 cut(s) 654
XceI RCATGY 3 cut(s) 149, 163, 224
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.