Rh2AG405000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018905
Physical Location & Seq
Reverse (-)
60374706 .. 60375297
592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG405000.1

Sequence Viewer

Length: 501 bp
ATGGCAAAACTGGTGGATGTTGGTGGTGGGACAGGAACAACCGTGGCCGAGATTGTGAAGGCGTATCCCAGCATTAAGGGGATCAACTTTGATCTACCATATGTGGTTGCAACAGCGCCGGCGTACCATAGGGTGTCACATGTCGGAGGTGACATGTTTGATGAGGGCAATATTCCAAACGCTGATGCAATTTTTATGAAGTGGGTTTTGCACGACTGGAGCGACAGCGATTGCATCAAGATATTGAAGAACTGTCGGAAGGCAATACCGGAGAGAAGTGGCAAGGTGATTATTGTGGACGTTGTTCTGGAGCCAAATGATGATGGCATGTTCGACGACACAGGCGTAGTTTTTGATCTGCTAATGATTGCTCATACCACAGGTGGAAAGGAGAGGAGCGAGAGTGAATGGAAGAAAATGTTGGAACAAGCAGGCTTCCCTCGCTACAAAATCATCAAAATTCCAGCTTTACCGTCCATTATCGAGGCCTACCCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

166

Amino Acids

18.25

Weight (kDa)

5.33

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 2 - 146 3.4e-51 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018905)

Species Orthologous Gene IDs
malus_domestica MD17G1079300.v1.1 MD17G1079400.v1.1
pyrus_communis pycom09g01480
rosa_chinensis RchiOBHm_Chr2g0162441
rosa_multiflora Rmu_co8443219.1_g000001
rosa_roxburghii Rroxscaffold_2G00087920
rosa_samantha Rh2AG405000 Rh2AG563600 Rh2BG576400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 459
AfaI GTAC 1 cut(s) 125
AflIII ACRYGT 2 cut(s) 139, 153
AgsI TTSAA 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AluBI AGCT 1 cut(s) 467
AluI AGCT 1 cut(s) 467
AlwI GGATC 1 cut(s) 89
AoxI GGCC 2 cut(s) 45, 486
ApoI RAATTY 1 cut(s) 459
AspLEI GCGC 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 161, 298
BccI CCATC 1 cut(s) 317
BciVI GTATCC 1 cut(s) 75
BfoI RGCGCY 1 cut(s) 119
BfuI GTATCC 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 312
BmsI GCATC 2 cut(s) 175, 243
BpmI CTGGAG 2 cut(s) 238, 329
BsaJI CCNNGG 1 cut(s) 42
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 15, 221
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 22
BseNI ACTGG 2 cut(s) 15, 221
BseRI GAGGAG 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 68
BshFI GGCC 2 cut(s) 47, 488
BsiSI CCGG 2 cut(s) 119, 269
BslFI GGGAC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 2 cut(s) 47, 488
Bsp143I GATC 3 cut(s) 81, 91, 355
BspANI GGCC 2 cut(s) 47, 488
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 2 cut(s) 15, 221
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 3 cut(s) 81, 91, 355
Bst4CI ACNGT 3 cut(s) 43, 254, 474
BstC8I GCNNGC 2 cut(s) 120, 433
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 3 cut(s) 84, 94, 358
BstMBI GATC 3 cut(s) 81, 91, 355
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstNSI RCATGY 3 cut(s) 143, 157, 331
BsuI GTATCC 1 cut(s) 75
BsuRI GGCC 2 cut(s) 47, 488
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 120, 433
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 118
Csp6I GTAC 1 cut(s) 124
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 3 cut(s) 140, 154, 328
CviJI RGCY 5 cut(s) 47, 313, 435, 467, 488
CviKI_1 RGCY 5 cut(s) 47, 313, 435, 467, 488
CviQI GTAC 1 cut(s) 124
DpnI GATC 3 cut(s) 83, 93, 357
DpnII GATC 3 cut(s) 81, 91, 355
EaeI YGGCCR 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 488
FaeI CATG 3 cut(s) 143, 157, 331
FaiI YATR 8 cut(s) 100, 102, 129, 141, 155, 197, 329, 375
FaqI GGGAC 1 cut(s) 43
FatI CATG 3 cut(s) 139, 153, 327
FauNDI CATATG 1 cut(s) 100
FokI GGATG 1 cut(s) 29
GlaI GCGC 1 cut(s) 117
GsaI CCCAGC 1 cut(s) 72
GsuI CTGGAG 2 cut(s) 238, 329
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 2 cut(s) 47, 488
HapII CCGG 2 cut(s) 119, 269
HhaI GCGC 1 cut(s) 118
Hin1II CATG 3 cut(s) 143, 157, 331
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HpaII CCGG 2 cut(s) 119, 269
HphI GGTGA 2 cut(s) 161, 298
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 146, 258
Hpy188III TCNNGA 2 cut(s) 238, 308
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 2 cut(s) 52, 253
HpyCH4III ACNGT 3 cut(s) 43, 254, 474
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 4 cut(s) 110, 188, 211, 234
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 3 cut(s) 143, 157, 331
HspAI GCGC 1 cut(s) 116
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Kzo9I GATC 3 cut(s) 81, 91, 355
LmnI GCTCC 3 cut(s) 219, 310, 396
LweI GCATC 2 cut(s) 175, 243
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 135, 149
MalI GATC 3 cut(s) 83, 93, 357
MboI GATC 3 cut(s) 81, 91, 355
MboII GAAGA 2 cut(s) 259, 424
MluCI AATT 2 cut(s) 189, 459
MmeI TCCRAC 3 cut(s) 124, 236, 402
MnlI CCTC 5 cut(s) 140, 157, 387, 450, 478
MreI CGCCGGCG 1 cut(s) 118
MroNI GCCGGC 1 cut(s) 118
MseI TTAA 1 cut(s) 75
MspI CCGG 2 cut(s) 119, 269
MwoI GCNNNNNNNGC 1 cut(s) 441
NaeI GCCGGC 1 cut(s) 120
NdeI CATATG 1 cut(s) 100
NdeII GATC 3 cut(s) 81, 91, 355
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 3 cut(s) 143, 157, 331
NlaIV GGNNCC 1 cut(s) 312
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 2 cut(s) 135, 149
NspI RCATGY 3 cut(s) 143, 157, 331
PceI AGGCCT 1 cut(s) 488
PciI ACATGT 2 cut(s) 139, 153
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 342
PdiI GCCGGC 1 cut(s) 120
PscI ACATGT 2 cut(s) 139, 153
PspFI CCCAGC 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 312
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 75
Sau3AI GATC 3 cut(s) 81, 91, 355
SetI ASST 5 cut(s) 151, 288, 303, 385, 469
SfaNI GCATC 2 cut(s) 175, 243
SgrAI CRCCGGYG 1 cut(s) 118
Sse9I AATT 2 cut(s) 189, 459
SseBI AGGCCT 1 cut(s) 488
SspI AATATT 1 cut(s) 172
StuI AGGCCT 1 cut(s) 488
TaaI ACNGT 3 cut(s) 43, 254, 474
TaiI ACGT 1 cut(s) 303
TaqI TCGA 2 cut(s) 333, 483
TasI AATT 2 cut(s) 189, 459
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TseFI GTSAC 2 cut(s) 135, 149
Tsp45I GTSAC 2 cut(s) 135, 149
TspDTI ATGAA 1 cut(s) 212
XapI RAATTY 1 cut(s) 459
XceI RCATGY 3 cut(s) 143, 157, 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.