MD17G1079400.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018905
Physical Location & Seq
Forward (+)
6554222 .. 6555108
887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1079400.v1.1.491

Sequence Viewer

Length: 480 bp
ATGTGGCTTTTGAGCAATGAGGAGCAAAGCCTTGCCCCACTAGTGCTTATGGCCAATCAGCCCCATCACATAGCCTCCTGGCATTGCATAAGCAAATGTATAACGGAAGGTGGAAGTGGGTTTGAAAAGGCAAATGGGTTTAGCCTCTATGATTTCTCAAGTGAGATATCTGAATTGGGAAACTACTTCAAAGAGGGTATGGCATGTACGTCTAGGATTGTCATGAAAGCAATATTGTCCAATTATAAAGATGGGTTTGAGGGAGTTGGATTGATTGCTCATGTTGGAGGGAGTATAGGGGCAGCTGTGGCTGAGATTGTAAATGCACATCCACATATAAGAGGCATCAATTTTGACCTACCAGATGTGGTGGCTAGGGCACCAAGGTATCCTGATGAGGGGATCCAATTCCACCAATTTGGGGGTGACATGTTTGATGCTATTCCCAATGCTGATGCCATTTTCATGAAGGTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

160

Amino Acids

17.32

Weight (kDa)

5.38

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 27 - 157 1.1e-28 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018905)

Species Orthologous Gene IDs
malus_domestica MD17G1079300.v1.1 MD17G1079400.v1.1
pyrus_communis pycom09g01480
rosa_chinensis RchiOBHm_Chr2g0162441
rosa_multiflora Rmu_co8443219.1_g000001
rosa_roxburghii Rroxscaffold_2G00087920
rosa_samantha Rh2AG405000 Rh2AG563600 Rh2BG576400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 379
AclWI GGATC 2 cut(s) 397, 410
AcoI YGGCCR 1 cut(s) 51
AfaI GTAC 1 cut(s) 208
AfiI CCNNNNNNNGG 2 cut(s) 398, 421
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 2 cut(s) 125, 190
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 1 cut(s) 77
AluBI AGCT 1 cut(s) 305
AluI AGCT 1 cut(s) 305
AlwI GGATC 2 cut(s) 397, 410
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 437
BaeGI GKGCMC 1 cut(s) 382
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BalI TGGCCA 1 cut(s) 53
BamHI GGATCC 1 cut(s) 402
BanI GGYRCC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 314
BccI CCATC 2 cut(s) 72, 245
BciT130I CCWGG 1 cut(s) 79
BciVI GTATCC 1 cut(s) 399
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 3 cut(s) 41, 213, 375
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 381, 404
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 3 cut(s) 354, 427, 445
BpuEI CTTGAG 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 383
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 421
Bse3DI GCAATG 2 cut(s) 22, 82
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 383
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 398, 421
BseMI GCAATG 2 cut(s) 22, 82
BseMII CTCAG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 1 cut(s) 314
BshFI GGCC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 398, 421
BsnI GGCC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 1 cut(s) 402
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 304
BspHI TCATGA 2 cut(s) 222, 465
BspLI GGNNCC 2 cut(s) 381, 404
BspPI GGATC 2 cut(s) 397, 410
BspT107I GGYRCC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 22, 82
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 383
Bst2UI CCWGG 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 1 cut(s) 405
BstMBI GATC 1 cut(s) 402
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 2 cut(s) 207, 433
BstSCI CCNGG 1 cut(s) 77
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 1 cut(s) 402
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 53
BtsCI GGATG 1 cut(s) 328
CciI TCATGA 2 cut(s) 222, 465
Csp6I GTAC 1 cut(s) 207
CviAII CATG 5 cut(s) 204, 223, 281, 430, 466
CviJI RGCY 9 cut(s) 7, 30, 53, 61, 74, 144, 305, 311, 374
CviKI_1 RGCY 9 cut(s) 7, 30, 53, 61, 74, 144, 305, 311, 374
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 1 cut(s) 312
DpnI GATC 1 cut(s) 404
DpnII GATC 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 383
Eco32I GATATC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 77
EcoRV GATATC 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 383
FaeI CATG 5 cut(s) 207, 226, 284, 433, 469
FatI CATG 5 cut(s) 203, 222, 280, 429, 465
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 303
FspBI CTAG 3 cut(s) 41, 213, 375
GluI GCNGC 1 cut(s) 303
HaeIII GGCC 1 cut(s) 53
Hin1II CATG 5 cut(s) 207, 226, 284, 433, 469
HphI GGTGA 1 cut(s) 437
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 3 cut(s) 223, 392, 466
HpyAV CCTTC 2 cut(s) 101, 463
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 2 cut(s) 87, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 5 cut(s) 207, 226, 284, 433, 469
Kzo9I GATC 1 cut(s) 402
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 4 cut(s) 64, 91, 375, 405
Lsp1109I GCAGC 1 cut(s) 314
LweI GCATC 3 cut(s) 354, 427, 445
MaeI CTAG 3 cut(s) 41, 213, 375
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 425
MalI GATC 1 cut(s) 404
MboI GATC 1 cut(s) 402
MflI RGATCY 1 cut(s) 402
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 5 cut(s) 173, 241, 349, 407, 416
MluNI TGGCCA 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 247, 265
MnlI CCTC 8 cut(s) 13, 85, 155, 187, 253, 281, 335, 391
Mox20I TGGCCA 1 cut(s) 53
MscI TGGCCA 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 464
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 1 cut(s) 402
NlaIII CATG 5 cut(s) 207, 226, 284, 433, 469
NlaIV GGNNCC 2 cut(s) 381, 404
NmuCI GTSAC 1 cut(s) 425
NspI RCATGY 2 cut(s) 207, 433
PagI TCATGA 2 cut(s) 222, 465
PciI ACATGT 1 cut(s) 429
PkrI GCNGC 1 cut(s) 304
PscI ACATGT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 381, 404
PsuI RGATCY 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
RseI CAYNNNNRTG 1 cut(s) 464
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 1 cut(s) 402
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 1 cut(s) 382
SetI ASST 6 cut(s) 112, 212, 307, 360, 389, 474
SfaNI GCATC 3 cut(s) 354, 427, 445
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 5 cut(s) 173, 241, 349, 407, 416
SspI AATATT 1 cut(s) 234
SspMI CTAG 3 cut(s) 41, 213, 375
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 383
TaiI ACGT 1 cut(s) 212
TasI AATT 5 cut(s) 173, 241, 349, 407, 416
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 2 cut(s) 239, 454
TspGWI ACGGA 1 cut(s) 119
XceI RCATGY 2 cut(s) 207, 433
XspI CTAG 3 cut(s) 41, 213, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.