MD17G1094400.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011586
Physical Location & Seq
Forward (+)
7952582 .. 7953160
579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1094400.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGAAACGGACAAACGGTCGCGCAGAGGCCTCAAAGTCTGCTGTGGTGCAACAGCAATTTTCCTAGTCATTGTGATCATCATCCTCACAACTCTTTCACTTACCATCCTGAAGCCTAAACAACCCATAATCGATGCCAAGCCTGTTGGCGTTCAAAACATTCAGTTTTCACTCTTCCCAAGTGCGACTCTAAATGTCACATTGAACATGCTTATTACAATAGAAAACAGGAACCGTATTGGGAGCTTCAAGTACAAGAATGCCACGGCTTATGTTCAGTACCGTGACAGCATTGTGGCCGAAGTTCCAATCCAGGCAGATTTTGTGCCTGCAAGAGGCAAGGTTAACATGACCACCTCGGTGGATCTTATGGGGGAGACCTTACTGTCGAACCCAAATTTCATGTCAGATGTGGCCAGTGGGAGCTTGAACATGACCTCAATTGCTAGTTTACATGGGAGAGTGAGTGTACTCAATTTCTTGAAGTTCCACGCGACGGCTTTAAGCACGTGTGACATCTCGTGTTCCATCCTAACCAAGACTATTGATTCTAAATGCAAGTCCAAAATGAAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

193

Amino Acids

21.02

Weight (kDa)

9.61

Isoelectric Point (pI)

29.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 72 - 156 9.4e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 385
AccII CGCG 2 cut(s) 22, 494
AclWI GGATC 1 cut(s) 372
AcoI YGGCCR 2 cut(s) 297, 414
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 131
AcvI CACGTG 1 cut(s) 510
AfaI GTAC 3 cut(s) 254, 281, 471
AfiI CCNNNNNNNGG 2 cut(s) 335, 496
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 5 cut(s) 155, 205, 250, 430, 484
AhdI GACNNNNNGTC 1 cut(s) 16
AjnI CCWGG 1 cut(s) 312
AjuI GAANNNNNNNTTGG 1 cut(s) 557
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 2 cut(s) 246, 426
AluI AGCT 2 cut(s) 246, 426
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 1 cut(s) 372
AoxI GGCC 3 cut(s) 28, 297, 414
ApoI RAATTY 1 cut(s) 397
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspLEI GCGC 1 cut(s) 24
BalI TGGCCA 1 cut(s) 416
BauI CACGAG 1 cut(s) 520
BbrPI CACGTG 1 cut(s) 510
BccI CCATC 2 cut(s) 113, 536
BceAI ACGGC 2 cut(s) 282, 513
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 65, 447
Bme1390I CCNGG 1 cut(s) 314
BmeRI GACNNNNNGTC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 1 cut(s) 124
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 80
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 264, 357
BsaXI ACNNNNNCTCC 2 cut(s) 451, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 496
Bse1I ACTGG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 264, 357
BseGI GGATG 3 cut(s) 81, 105, 528
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 2 cut(s) 335, 496
BseNI ACTGG 1 cut(s) 417
Bsh1236I CGCG 2 cut(s) 22, 494
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 3 cut(s) 30, 299, 416
BshVI ATCGAT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 335, 496
BsmAI GTCTC 1 cut(s) 371
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 3 cut(s) 30, 299, 416
Bso31I GGTCTC 1 cut(s) 371
Bsp143I GATC 2 cut(s) 75, 364
BspANI GGCC 3 cut(s) 30, 299, 416
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 2 cut(s) 22, 494
BspLI GGNNCC 1 cut(s) 233
BspPI GGATC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 371
BsrI ACTGG 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 264, 357
BssMI GATC 2 cut(s) 75, 364
BssSI CACGAG 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 520
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 4 cut(s) 18, 236, 284, 387
Bst6I CTCTTC 1 cut(s) 179
BstBAI YACGTR 1 cut(s) 510
BstC8I GCNNGC 1 cut(s) 330
BstDSI CCRYGG 1 cut(s) 264
BstENI CCTNNNNNAGG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 81, 105, 528
BstFNI CGCG 2 cut(s) 22, 494
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 2 cut(s) 78, 367
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 2 cut(s) 75, 364
BstMCI CGRYCG 1 cut(s) 20
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 312
BstUI CGCG 2 cut(s) 22, 494
BstX2I RGATCY 1 cut(s) 364
BstXI CCANNNNNNTGG 1 cut(s) 361
BstYI RGATCY 1 cut(s) 364
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 3 cut(s) 30, 299, 416
BsuTUI ATCGAT 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 3 cut(s) 81, 105, 528
BtsIMutI CAGTG 1 cut(s) 424
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 24
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 3 cut(s) 253, 280, 470
CviAII CATG 5 cut(s) 208, 349, 403, 433, 455
CviJI RGCY 9 cut(s) 30, 115, 142, 246, 269, 299, 416, 426, 500
CviKI_1 RGCY 9 cut(s) 30, 115, 142, 246, 269, 299, 416, 426, 500
CviQI GTAC 3 cut(s) 253, 280, 470
DpnI GATC 2 cut(s) 77, 366
DpnII GATC 2 cut(s) 75, 364
DrdI GACNNNNNNGTC 1 cut(s) 385
DriI GACNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 2 cut(s) 297, 414
Eam1104I CTCTTC 1 cut(s) 179
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 179
Eco147I AGGCCT 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 131
Eco72I CACGTG 1 cut(s) 510
EcoNI CCTNNNNNAGG 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 312
FaeI CATG 5 cut(s) 211, 352, 406, 436, 458
FaiI YATR 8 cut(s) 128, 209, 273, 350, 371, 404, 434, 456
FatI CATG 5 cut(s) 207, 348, 402, 432, 454
FbaI TGATCA 1 cut(s) 75
FokI GGATG 3 cut(s) 68, 92, 515
FspBI CTAG 2 cut(s) 65, 447
GlaI GCGC 1 cut(s) 23
HaeIII GGCC 3 cut(s) 30, 299, 416
HhaI GCGC 1 cut(s) 24
Hin1II CATG 5 cut(s) 211, 352, 406, 436, 458
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 2 cut(s) 187, 548
HpaI GTTAAC 1 cut(s) 346
Hpy166II GTNNAC 3 cut(s) 346, 452, 470
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 2 cut(s) 109, 481
Hpy8I GTNNAC 3 cut(s) 346, 452, 470
Hpy99I CGWCG 1 cut(s) 499
HpyCH4III ACNGT 4 cut(s) 18, 236, 284, 387
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 3 cut(s) 50, 332, 558
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 5 cut(s) 211, 352, 406, 436, 458
HspAI GCGC 1 cut(s) 22
Ksp22I TGATCA 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 346
Kzo9I GATC 2 cut(s) 75, 364
LmnI GCTCC 2 cut(s) 243, 423
LpnPI CCDG 7 cut(s) 122, 156, 214, 299, 326, 342, 430
LweI GCATC 1 cut(s) 124
MaeI CTAG 2 cut(s) 65, 447
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 3 cut(s) 196, 284, 512
MalI GATC 2 cut(s) 77, 366
MboI GATC 2 cut(s) 75, 364
MboII GAAGA 1 cut(s) 166
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 364
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 4 cut(s) 57, 397, 441, 475
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 6 cut(s) 20, 41, 95, 329, 367, 448
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
MseI TTAA 2 cut(s) 345, 503
MslI CAYNNNNRTG 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 314
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 2 cut(s) 22, 494
NdeII GATC 2 cut(s) 75, 364
NlaIII CATG 5 cut(s) 211, 352, 406, 436, 458
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 3 cut(s) 196, 284, 512
NspI RCATGY 1 cut(s) 211
OliI CACNNNNGTG 1 cut(s) 359
PceI AGGCCT 1 cut(s) 30
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 1 cut(s) 548
PleI GAGTC 1 cut(s) 181
PmaCI CACGTG 1 cut(s) 510
PmlI CACGTG 1 cut(s) 510
PpsI GAGTC 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 312
PspCI CACGTG 1 cut(s) 510
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 233
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 3 cut(s) 254, 281, 471
RsaNI GTAC 3 cut(s) 253, 280, 470
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 2 cut(s) 345, 503
Sau3AI GATC 2 cut(s) 75, 364
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 314
SetI ASST 7 cut(s) 248, 345, 359, 383, 428, 440, 512
SfaNI GCATC 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 359
Sse9I AATT 4 cut(s) 57, 397, 441, 475
SseBI AGGCCT 1 cut(s) 30
SspMI CTAG 2 cut(s) 65, 447
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 312
TaaI ACNGT 4 cut(s) 18, 236, 284, 387
TaiI ACGT 1 cut(s) 512
TaqI TCGA 2 cut(s) 132, 389
TasI AATT 4 cut(s) 57, 397, 441, 475
TatI WGTACW 2 cut(s) 252, 469
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 2 cut(s) 345, 503
Tru9I TTAA 2 cut(s) 345, 503
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 3 cut(s) 196, 284, 512
Tsp45I GTSAC 3 cut(s) 196, 284, 512
TspDTI ATGAA 1 cut(s) 391
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 424
XagI CCTNNNNNAGG 1 cut(s) 333
XapI RAATTY 1 cut(s) 397
XceI RCATGY 1 cut(s) 211
XspI CTAG 2 cut(s) 65, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.