Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0015190

Physical Location & Seq

Genomic Coordinates Reverse (-)

12202012 .. 12208475

Gene Structure 6464 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1136200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGGCGAACGTCTCGGTTGCCGCGGAGTGGCAGCTCCTCTACAACCGCTACTACCGTAAGCCCGAAATCTACCGAATGAGCTGGAAGCACGTAGAGCTCAACCGCAACAAGGTCGCCTGCGCCCCCTTCGGCGGTCCCATCGCCGTCATCCGAGACGATTCCAAGATCGTCCAACTCGGCGGCGAATCGGCCCAGCGGAAGCTCCGCATCTTCAGTTCCGCTGGCCATCTTCTCGGCGAGACAATCTGGAAACACCCGGGCGGCCGATTAATCGGCATGGCCTGGACCGACGACCAGAACCTAGTCTGCCTCGTCCAAGACGGCACCGTTTTTCGCTACACCATCCACGCGGAGCTCGTCGAGCCAAGTATCTCCATGGGGAAAGAGTGCTTCGAGCGAAACGTCGTGGACTGCGTTTTCTGGGGAAACGGCGTCGTTTGTATCACCGAGACGAATCAGCTGTTCTGCATTTCCGATTTCAAGAACCCGAACCCGATTAAGCTTGCCGACCCGGGGATCGAGGACCCGCCGCTCTGTATGGCGGTGATCGAGCCGCAGTACACGATGTCGGGAAATGTGGAGGTTCTGCTCGGGATCGGAGATGCTTGCGTTTTGGCTGTGGAGGAAGACGGGGTTCAGCAGCTAGGGCTGGAGATGCTCCGCGGGCCGATTCAGAAGATGGCGGTTTCGAGAGACGGGCAGTGGCTTGCTTCCTTCACGCACGACGGTCGTTTGTTGGTGATGACTTCGAATTTGAACGACATTCTCATTGAGCAGGAATGTGAGTCGGCTCTTCCTCCGGAACAGCTCTCTTGGTGTGGAATGGACACTGTGCTGCTTTATTGGGATGATATGCTTTTGATGATGGGTCCTAGAGGTGATCCAGTACGCTATTTTTATGATGAACCAATAATTCTTATTCCCGAGTGCGATGGAGTGAGGATATTATCTAACTCGAGCATGGAGTTTCTACAACGGGTGCCTGATTCCACTGAATCCATCTTCAAGATTGGAAGTACATCGCCTGCAGCTTTGTTGTATGATGCTTTGGACCATTTCGACAGACGAAGTGCCAAGGCAGATGAGAATTTGCGACTAATACGCCCATCCTTGACGGAGGCTGTTGAAGCATGTATTGATGCTGCTGGCCATGAATTTGATGTTCTACGCCAACGAACACTCCTAAGAGCTGCAAGCTATGGCCAAGCCTTTTGCAGCAATTTTCAACGTGATCATATTCAAGAGATGTGTAAAACTTTGCGGGTATTAAATGCTGTTCGGCATCCTGACGTTGGAATGCCTCTAAGTATTCAACAATACAAGTTACTTACACCAGCAGTTCTGATCAGTCGTTTGATCAATGCCTACAAACACTTTCTGGCACTACGGATATCAGAGTACCTTGGAATGAACCAAGAGATGGTGATTATGCACTGGGCATGCTCCAAAATAACAGCATCCTTAGCAATTTCTGATACCAATCTCCTTGATATTTTGCTTGATAAGTTGAAACTATGCAAAGGAATATCCTATGCAGCAGTTGCTGCTCATGCAGATAAGAATGGCCGCCGGAAGCTAGCTGCCATGCTTGTTGAGCATGAACCACGCTCCTCCAAACAGGTTCCTCTGTTGTTGAGCATAGGAGAAGAAGATACAGCTTTAATGAAAGCAATTGAAAGTGGTGATACAGACCTTGTTTATCTTGTTCTCTTTCATATCTGGCAAAAGCGACAACCATTGGAGTTCTTTGGAATGATACAAGCTAGAGCTCTGGCACGCGATTTGTTTATAATTTATGCACGGTGCTACAAGCATGAATTCTTGAAGGACTTTTTTCTATCAACAGGACAACTTCAAGAGGTGGCTTTCCTTCTGTGGAAAGAATCTTGGGAGATTGGAAAAAATCCAATGGCAAGCAGAGGATCTCCCCTTCATGGTCCACGCATAAAACTAATTGAGAAGGCCCATAATCTTTTCGTAGAAACTAAGGAATATACCTTTGAGTCGAAGGCTGCTGAAGAACATGCAAAGCTTATAAGGATGCAGCATGAGTTAGAAGTCTCAACAAAGCAGGCCATTTTTGTTGACTCGAGTATCAGTGATACAATTAGAACTTGTATTGTTCTGGGAAATCATCGAGCTGCAATGAAAGTGAAAACAGAATTCAAGGTTTCTGAGAAGAGATGGTATTGGCTAAAAGTTTTTGCTTTGGCTACAATCAGAGATTGGGATGCCTTGGAGAAGTTCTCAAAGGAAAAGAGACCACCAATTGGTTACAGGCCATTTGTGGAGGCGTGTGTTGACGCAGATGAGAAAGGAGAGGCTTTGAAATATATCCCAAAACTCACAGATCCTCGGGAAAGAGCGGAGTCTTATGCTCGGATTGGCATGGCCAAGGAAGCTGCTGATGCTGCCTCTCAGGCAAAAGATGGTGAATTGCTTGGTCGACTCAAAATGACTTTCTCACAAAATGCTGCAGCCTCATCAATTTTTGATACTCTTAGGGACCGATTCGTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000325 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005856 GO:0006810 GO:0006906 GO:0006996 GO:0007033 GO:0008092 GO:0008150 GO:0009705 GO:0009987 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016197 GO:0030897 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032879 GO:0032889 GO:0032991 GO:0033043 GO:0033263 GO:0035542 GO:0042144 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0044087 GO:0044088 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045992 GO:0045995 GO:0046907 GO:0048284 GO:0048519 GO:0050789 GO:0050793 GO:0050794 GO:0051049 GO:0051093 GO:0051128 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051469 GO:0051641 GO:0051649 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0071840 GO:0090174 GO:0097576 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:0099023 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

Vps16_C Vps16_N

Protein Analysis

841

Amino Acids

94.92

Weight (kDa)

6.11

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps16_N	PF04841	8 - 412	3.8e-84	Vps16, N-terminal region
Vps16_C	PF04840	509 - 822	2.4e-68	Vps16, C-terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015190)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38020 AT2G38020
fragaria_vesca	FvH4_6g38590 FvH4_6g38590
malus_domestica	MD17G1136200.v1.1
prunus_persica	Prupe.3G176600_v2.0.a1 Prupe.3G176600_v2.0.a1
pyrus_communis	pycom17g12990
rosa_chinensis	RchiOBHm_Chr2g0152001
rosa_laevigata	RLG00000020599
rosa_multiflora	Rmu_sc0017048.1_g000002
rosa_roxburghii	Rroxscaffold_2G00096060
rosa_rugosa	Rorug02G0430200 Rorug02G0430300
rosa_samantha	Rh2BG503200
rosa_wichuraiana	Rw2G040350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1790, 2036
AccB1I	GGYRCC	2 cut(s)	323, 979
AccB7I	CCANNNNNTGG	2 cut(s)	1420, 2270
AccBSI	CCGCTC	2 cut(s)	532, 2366
AccI	GTMKAC	1 cut(s)	2446
AccII	CGCG	4 cut(s)	23, 350, 663, 1779
AccIII	TCCGGA	1 cut(s)	799
AclWI	GGATC	5 cut(s)	524, 602, 875, 1930, 2345
AcoI	YGGCCR	6 cut(s)	223, 262, 1147, 1201, 1564, 2391
AcsI	RAATTY	5 cut(s)	751, 1087, 1154, 1817, 2162
AcuI	CTGAAG	2 cut(s)	196, 2037
AcyI	GRCGYC	1 cut(s)	432
AfaI	GTAC	4 cut(s)	560, 888, 1018, 1400
AfiI	CCNNNNNNNGG	8 cut(s)	27, 109, 131, 513, 1292, 1420, 1934, 2270
AjnI	CCWGG	1 cut(s)	281
Alw21I	GWGCWC	3 cut(s)	99, 357, 1771
Alw26I	GTCTC	7 cut(s)	16, 147, 233, 443, 687, 2065, 2254
AlwI	GGATC	5 cut(s)	524, 602, 875, 1930, 2345
AlwNI	CAGNNNCTG	1 cut(s)	1544
Ama87I	CYCGRG	7 cut(s)	256, 511, 590, 923, 955, 2089, 2355
Aor13HI	TCCGGA	1 cut(s)	799
ApoI	RAATTY	5 cut(s)	751, 1087, 1154, 1817, 2162
ArsI	GACNNNNNNTTYG	2 cut(s)	736, 768
AseI	ATTAAT	1 cut(s)	269
AspLEI	GCGC	1 cut(s)	122
AsuC2I	CCSGG	4 cut(s)	257, 258, 512, 513
AsuHPI	GGTGA	7 cut(s)	436, 556, 751, 890, 1435, 1694, 2444
AsuII	TTCGAA	1 cut(s)	749
AsuNHI	GCTAGC	1 cut(s)	1576
AvaI	CYCGRG	7 cut(s)	256, 511, 590, 923, 955, 2089, 2355
AvaII	GGWCC	7 cut(s)	134, 285, 523, 869, 1051, 1937, 2506
BaeI	ACNNNNGTAYC	2 cut(s)	2077, 2110
BalI	TGGCCA	4 cut(s)	225, 1149, 1203, 2393
BanI	GGYRCC	2 cut(s)	323, 979
BanII	GRGCYC	3 cut(s)	99, 357, 1771
BbsI	GAAGAC	1 cut(s)	633
Bbv12I	GWGCWC	3 cut(s)	99, 357, 1771
BceAI	ACGGC	3 cut(s)	128, 337, 445
BcgI	CGANNNNNNTGC	4 cut(s)	94, 128, 710, 744
BciT130I	CCWGG	1 cut(s)	283
BclI	TGATCA	3 cut(s)	1231, 1344, 1356
BcnI	CCSGG	4 cut(s)	257, 258, 512, 513
BcoDI	GTCTC	7 cut(s)	16, 147, 233, 443, 687, 2065, 2254
BfaI	CTAG	5 cut(s)	302, 644, 873, 1577, 1764
BfmI	CTRYAG	2 cut(s)	1026, 2475
BglI	GCCNNNNNGGC	1 cut(s)	2420
Bme1390I	CCNGG	5 cut(s)	257, 258, 283, 512, 513
Bme18I	GGWCC	7 cut(s)	134, 285, 523, 869, 1051, 1937, 2506
BmeT110I	CYCGRG	7 cut(s)	256, 511, 590, 923, 955, 2089, 2355
BmiI	GGNNCC	7 cut(s)	136, 325, 525, 870, 981, 1623, 2507
BmrFI	CCNGG	5 cut(s)	257, 258, 283, 512, 513
BmrI	ACTGGG	1 cut(s)	1444
BmtI	GCTAGC	1 cut(s)	1580
BmuI	ACTGGG	1 cut(s)	1444
BpiI	GAAGAC	1 cut(s)	633
BplI	GAGNNNNNCTC	2 cut(s)	2231, 2263
BpmI	CTGGAG	1 cut(s)	671
Bpu10I	CCTNAGC	1 cut(s)	1462
Bpu14I	TTCGAA	1 cut(s)	749
BpuMI	CCSGG	4 cut(s)	257, 258, 512, 513
BsaAI	YACGTR	1 cut(s)	91
BsaBI	GATNNNNATC	1 cut(s)	1479
BsaHI	GRCGYC	1 cut(s)	432
BsaI	GGTCTC	1 cut(s)	2254
BsaWI	WCCGGW	1 cut(s)	799
BsaXI	ACNNNNNCTCC	2 cut(s)	1164, 1194
Bsc4I	CCNNNNNNNGG	8 cut(s)	27, 109, 131, 513, 1292, 1420, 1934, 2270
Bse1I	ACTGG	2 cut(s)	884, 1439
Bse3DI	GCAATG	1 cut(s)	2151
Bse8I	GATNNNNATC	1 cut(s)	1479
BseAI	TCCGGA	1 cut(s)	799
BseBI	CCWGG	1 cut(s)	283
BseGI	GGATG	8 cut(s)	147, 342, 853, 1106, 1282, 1457, 2046, 2236
BseJI	GATNNNNATC	1 cut(s)	1479
BseLI	CCNNNNNNNGG	8 cut(s)	27, 109, 131, 513, 1292, 1420, 1934, 2270
BseMI	GCAATG	1 cut(s)	2151
BseMII	CTCAG	2 cut(s)	2166, 2432
BseNI	ACTGG	2 cut(s)	884, 1439
BseRI	GAGGAG	2 cut(s)	26, 1600
BseX3I	CGGCCG	1 cut(s)	262
BseYI	CCCAGC	1 cut(s)	192
Bsh1236I	CGCG	4 cut(s)	23, 350, 663, 1779
Bsh1285I	CGRYCG	2 cut(s)	265, 730
BshNI	GGYRCC	2 cut(s)	323, 979
BsiEI	CGRYCG	2 cut(s)	265, 730
BsiHKAI	GWGCWC	3 cut(s)	99, 357, 1771
BsiHKCI	CYCGRG	7 cut(s)	256, 511, 590, 923, 955, 2089, 2355
BsiSI	CCGG	4 cut(s)	257, 512, 800, 1570
BslFI	GGGAC	2 cut(s)	120, 2519
BslI	CCNNNNNNNGG	8 cut(s)	27, 109, 131, 513, 1292, 1420, 1934, 2270
BsmAI	GTCTC	7 cut(s)	16, 147, 233, 443, 687, 2065, 2254
BsmBI	CGTCTC	4 cut(s)	16, 147, 443, 687
BsmFI	GGGAC	2 cut(s)	120, 2519
BsmI	GAATGC	1 cut(s)	1302
Bso31I	GGTCTC	1 cut(s)	2254
BsoBI	CYCGRG	7 cut(s)	256, 511, 590, 923, 955, 2089, 2355
Bsp119I	TTCGAA	1 cut(s)	749
Bsp1286I	GDGCHC	3 cut(s)	99, 357, 1771
Bsp13I	TCCGGA	1 cut(s)	799
Bsp19I	CCATGG	1 cut(s)	375
BspCNI	CTCAG	2 cut(s)	2167, 2431
BspEI	TCCGGA	1 cut(s)	799
BspFNI	CGCG	4 cut(s)	23, 350, 663, 1779
BspLI	GGNNCC	7 cut(s)	136, 325, 525, 870, 981, 1623, 2507
BspMAI	CTGCAG	2 cut(s)	1030, 2479
BspOI	GCTAGC	1 cut(s)	1580
BspPI	GGATC	5 cut(s)	524, 602, 875, 1930, 2345
BspQI	GCTCTTC	1 cut(s)	798
BspT104I	TTCGAA	1 cut(s)	749
BspT107I	GGYRCC	2 cut(s)	323, 979
BspTNI	GGTCTC	1 cut(s)	2254
BsrBI	CCGCTC	2 cut(s)	532, 2366
BsrDI	GCAATG	1 cut(s)	2151
BsrI	ACTGG	2 cut(s)	884, 1439
BssNI	GRCGYC	1 cut(s)	432
BssT1I	CCWWGG	5 cut(s)	375, 1074, 1402, 2235, 2394
Bst2UI	CCWGG	1 cut(s)	283
Bst4CI	ACNGT	5 cut(s)	56, 328, 728, 832, 1803
Bst6I	CTCTTC	2 cut(s)	798, 2174
BstACI	GRCGYC	1 cut(s)	432
BstAPI	GCANNNNNTGC	2 cut(s)	1541, 1544
BstBAI	YACGTR	1 cut(s)	91
BstBI	TTCGAA	1 cut(s)	749
BstDEI	CTNAG	7 cut(s)	1184, 1304, 1462, 1986, 2175, 2418, 2501
BstDSI	CCRYGG	3 cut(s)	21, 375, 661
BstF5I	GGATG	8 cut(s)	147, 342, 853, 1106, 1282, 1457, 2046, 2236
BstFNI	CGCG	4 cut(s)	23, 350, 663, 1779
BstHHI	GCGC	1 cut(s)	122
BstMAI	GTCTC	7 cut(s)	16, 147, 233, 443, 687, 2065, 2254
BstMCI	CGRYCG	2 cut(s)	265, 730
BstNI	CCWGG	1 cut(s)	283
BstNSI	RCATGY	3 cut(s)	1134, 1443, 2027
BstSCI	CCNGG	5 cut(s)	255, 256, 281, 510, 511
BstSFI	CTRYAG	2 cut(s)	1026, 2475
BstUI	CGCG	4 cut(s)	23, 350, 663, 1779
BstV2I	GAAGAC	1 cut(s)	633
BstX2I	RGATCY	2 cut(s)	1922, 2350
BstYI	RGATCY	2 cut(s)	1922, 2350
BstZI	CGGCCG	1 cut(s)	262
BtgI	CCRYGG	3 cut(s)	21, 375, 661
BtgZI	GCGATG	3 cut(s)	124, 945, 1005
BtsCI	GGATG	8 cut(s)	147, 342, 853, 1106, 1282, 1457, 2046, 2236
BtsI	GCAGTG	1 cut(s)	707
BtsIMutI	CAGTG	5 cut(s)	707, 828, 990, 1432, 2104
CaiI	CAGNNNCTG	1 cut(s)	1544
CfoI	GCGC	1 cut(s)	122
Cfr42I	CCGCGG	2 cut(s)	24, 664
Cfr9I	CCCGGG	2 cut(s)	256, 511
CseI	GACGC	2 cut(s)	421, 2312
Csp6I	GTAC	4 cut(s)	559, 887, 1017, 1399
CviQI	GTAC	4 cut(s)	559, 887, 1017, 1399
DdeI	CTNAG	7 cut(s)	1184, 1304, 1462, 1986, 2175, 2418, 2501
EaeI	YGGCCR	6 cut(s)	223, 262, 1147, 1201, 1564, 2391
EagI	CGGCCG	1 cut(s)	262
Eam1104I	CTCTTC	2 cut(s)	798, 2174
EarI	CTCTTC	2 cut(s)	798, 2174
Ecl136II	GAGCTC	3 cut(s)	97, 355, 1769
EclXI	CGGCCG	1 cut(s)	262
Eco130I	CCWWGG	5 cut(s)	375, 1074, 1402, 2235, 2394
Eco24I	GRGCYC	3 cut(s)	99, 357, 1771
Eco31I	GGTCTC	1 cut(s)	2254
Eco32I	GATATC	1 cut(s)	1392
Eco47I	GGWCC	7 cut(s)	134, 285, 523, 869, 1051, 1937, 2506
Eco52I	CGGCCG	1 cut(s)	262
Eco53kI	GAGCTC	3 cut(s)	97, 355, 1769
Eco57I	CTGAAG	2 cut(s)	196, 2037
Eco88I	CYCGRG	7 cut(s)	256, 511, 590, 923, 955, 2089, 2355
EcoICRI	GAGCTC	3 cut(s)	97, 355, 1769
EcoO109I	RGGNCCY	2 cut(s)	523, 869
EcoRI	GAATTC	2 cut(s)	1817, 2162
EcoRII	CCWGG	1 cut(s)	281
EcoRV	GATATC	1 cut(s)	1392
EcoT14I	CCWWGG	5 cut(s)	375, 1074, 1402, 2235, 2394
EcoT38I	GRGCYC	3 cut(s)	99, 357, 1771
ErhI	CCWWGG	5 cut(s)	375, 1074, 1402, 2235, 2394
Esp3I	CGTCTC	4 cut(s)	16, 147, 443, 687
FaqI	GGGAC	2 cut(s)	120, 2519
FauI	CCCGC	3 cut(s)	534, 656, 1254
FbaI	TGATCA	3 cut(s)	1231, 1344, 1356
FblI	GTMKAC	1 cut(s)	2446
FokI	GGATG	8 cut(s)	134, 329, 860, 1093, 1269, 1444, 2053, 2243
FriOI	GRGCYC	3 cut(s)	99, 357, 1771
FspBI	CTAG	5 cut(s)	302, 644, 873, 1577, 1764
GlaI	GCGC	1 cut(s)	121
GsaI	CCCAGC	1 cut(s)	196
GsuI	CTGGAG	1 cut(s)	671
HapII	CCGG	4 cut(s)	257, 512, 800, 1570
HgaI	GACGC	2 cut(s)	421, 2312
HhaI	GCGC	1 cut(s)	122
Hin1I	GRCGYC	1 cut(s)	432
Hin6I	GCGC	1 cut(s)	120
HinP1I	GCGC	1 cut(s)	120
HincII	GTYRAC	3 cut(s)	2086, 2302, 2447
HindII	GTYRAC	3 cut(s)	2086, 2302, 2447
HindIII	AAGCTT	2 cut(s)	500, 2030
HpaII	CCGG	4 cut(s)	257, 512, 800, 1570
HphI	GGTGA	7 cut(s)	436, 556, 751, 890, 1435, 1694, 2444
Hpy166II	GTNNAC	6 cut(s)	409, 561, 1940, 2086, 2302, 2447
Hpy8I	GTNNAC	6 cut(s)	409, 561, 1940, 2086, 2302, 2447
Hpy99I	CGWCG	5 cut(s)	293, 362, 407, 437, 728
HpyAV	CCTTC	7 cut(s)	136, 724, 1819, 1880, 1940, 1954, 2002
HpyCH4III	ACNGT	5 cut(s)	56, 328, 728, 832, 1803
HpyCH4IV	ACGT	5 cut(s)	9, 90, 402, 1228, 1290
HpyF3I	CTNAG	7 cut(s)	1184, 1304, 1462, 1986, 2175, 2418, 2501
HpySE526I	ACGT	5 cut(s)	9, 90, 402, 1228, 1290
Hsp92I	GRCGYC	1 cut(s)	432
HspAI	GCGC	1 cut(s)	120
Kpn2I	TCCGGA	1 cut(s)	799
Ksp22I	TGATCA	3 cut(s)	1231, 1344, 1356
KspI	CCGCGG	2 cut(s)	24, 664
LguI	GCTCTTC	1 cut(s)	798
LmnI	GCTCC	6 cut(s)	39, 207, 352, 663, 1448, 1613
MaeI	CTAG	5 cut(s)	302, 644, 873, 1577, 1764
MaeII	ACGT	5 cut(s)	9, 90, 402, 1228, 1290
MaeIII	GTNAC	2 cut(s)	1323, 2273
MbiI	CCGCTC	2 cut(s)	532, 2366
MfeI	CAATTG	2 cut(s)	1671, 2268
MflI	RGATCY	2 cut(s)	1922, 2350
MhlI	GDGCHC	3 cut(s)	99, 357, 1771
MlsI	TGGCCA	4 cut(s)	225, 1149, 1203, 2393
MluNI	TGGCCA	4 cut(s)	225, 1149, 1203, 2393
MlyI	GAGTC	5 cut(s)	794, 2012, 2081, 2378, 2442
MmeI	TCCRAC	2 cut(s)	196, 1273
Mox20I	TGGCCA	4 cut(s)	225, 1149, 1203, 2393
MroI	TCCGGA	1 cut(s)	799
MscI	TGGCCA	4 cut(s)	225, 1149, 1203, 2393
MseI	TTAA	4 cut(s)	269, 498, 1268, 1661
Msp20I	TGGCCA	4 cut(s)	225, 1149, 1203, 2393
MspA1I	CMGCKG	5 cut(s)	23, 196, 221, 460, 663
MspI	CCGG	4 cut(s)	257, 512, 800, 1570
MspR9I	CCNGG	5 cut(s)	257, 258, 283, 512, 513
MunI	CAATTG	2 cut(s)	1671, 2268
Mva1269I	GAATGC	1 cut(s)	1302
MvaI	CCWGG	1 cut(s)	283
MvnI	CGCG	4 cut(s)	23, 350, 663, 1779
NciI	CCSGG	4 cut(s)	257, 258, 512, 513
NcoI	CCATGG	1 cut(s)	375
NheI	GCTAGC	1 cut(s)	1576
NlaIV	GGNNCC	7 cut(s)	136, 325, 525, 870, 981, 1623, 2507
NmeAIII	GCCGAG	2 cut(s)	156, 213
NspI	RCATGY	3 cut(s)	1134, 1443, 2027
NspV	TTCGAA	1 cut(s)	749
PaeI	GCATGC	1 cut(s)	1443
PaeR7I	CTCGAG	2 cut(s)	955, 2089
PciSI	GCTCTTC	1 cut(s)	798
PcsI	WCGNNNNNNNCGW	3 cut(s)	354, 399, 411
PctI	GAATGC	1 cut(s)	1302
PfeI	GAWTC	8 cut(s)	158, 185, 454, 670, 986, 995, 1883, 2511
PflMI	CCANNNNNTGG	2 cut(s)	1420, 2270
PleI	GAGTC	5 cut(s)	793, 2011, 2081, 2377, 2442
PpsI	GAGTC	5 cut(s)	793, 2011, 2081, 2377, 2442
Ppu21I	YACGTR	1 cut(s)	91
PpuMI	RGGWCCY	2 cut(s)	523, 869
PshBI	ATTAAT	1 cut(s)	269
PsiI	TTATAA	2 cut(s)	1790, 2036
Psp124BI	GAGCTC	3 cut(s)	99, 357, 1771
Psp5II	RGGWCCY	2 cut(s)	523, 869
Psp6I	CCWGG	1 cut(s)	281
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	1 cut(s)	281
PspN4I	GGNNCC	7 cut(s)	136, 325, 525, 870, 981, 1623, 2507
PspPPI	RGGWCCY	2 cut(s)	523, 869
PspXI	VCTCGAGB	2 cut(s)	955, 2089
PstI	CTGCAG	2 cut(s)	1030, 2479
PstNI	CAGNNNCTG	1 cut(s)	1544
PsuI	RGATCY	2 cut(s)	1922, 2350
PvuII	CAGCTG	1 cut(s)	460
RsaI	GTAC	4 cut(s)	560, 888, 1018, 1400
RsaNI	GTAC	4 cut(s)	559, 887, 1017, 1399
SacI	GAGCTC	3 cut(s)	99, 357, 1771
SacII	CCGCGG	2 cut(s)	24, 664
SalI	GTCGAC	1 cut(s)	2445
SapI	GCTCTTC	1 cut(s)	798
SaqAI	TTAA	4 cut(s)	269, 498, 1268, 1661
SchI	GAGTC	5 cut(s)	794, 2012, 2081, 2378, 2442
ScrFI	CCNGG	5 cut(s)	257, 258, 283, 512, 513
SduI	GDGCHC	3 cut(s)	99, 357, 1771
SfcI	CTRYAG	2 cut(s)	1026, 2475
Sfr274I	CTCGAG	2 cut(s)	955, 2089
Sfr303I	CCGCGG	2 cut(s)	24, 664
SfuI	TTCGAA	1 cut(s)	749
SgrBI	CCGCGG	2 cut(s)	24, 664
SinI	GGWCC	7 cut(s)	134, 285, 523, 869, 1051, 1937, 2506
SlaI	CTCGAG	2 cut(s)	955, 2089
SmaI	CCCGGG	2 cut(s)	258, 513
SmlI	CTYRAG	2 cut(s)	955, 2089
SmoI	CTYRAG	2 cut(s)	955, 2089
SphI	GCATGC	1 cut(s)	1443
SspMI	CTAG	5 cut(s)	302, 644, 873, 1577, 1764
SstI	GAGCTC	3 cut(s)	99, 357, 1771
StyD4I	CCNGG	5 cut(s)	255, 256, 281, 510, 511
StyI	CCWWGG	5 cut(s)	375, 1074, 1402, 2235, 2394
TaaI	ACNGT	5 cut(s)	56, 328, 728, 832, 1803
TaiI	ACGT	5 cut(s)	12, 93, 405, 1231, 1293
TaqII	GACCGA	2 cut(s)	302, 2523
TatI	WGTACW	2 cut(s)	558, 1016
TauI	GCSGC	6 cut(s)	23, 183, 264, 532, 556, 1569
TfiI	GAWTC	8 cut(s)	158, 185, 454, 670, 986, 995, 1883, 2511
Tru1I	TTAA	4 cut(s)	269, 498, 1268, 1661
Tru9I	TTAA	4 cut(s)	269, 498, 1268, 1661
TscAI	CASTG	5 cut(s)	707, 835, 997, 1439, 2104
TspGWI	ACGGA	2 cut(s)	1130, 1402
TspMI	CCCGGG	2 cut(s)	256, 511
TspRI	CASTG	5 cut(s)	707, 835, 997, 1439, 2104
Van91I	CCANNNNNTGG	2 cut(s)	1420, 2270
VpaK11BI	GGWCC	7 cut(s)	134, 285, 523, 869, 1051, 1937, 2506
VspI	ATTAAT	1 cut(s)	269
XapI	RAATTY	5 cut(s)	751, 1087, 1154, 1817, 2162
XceI	RCATGY	3 cut(s)	1134, 1443, 2027
XhoI	CTCGAG	2 cut(s)	955, 2089
XmaI	CCCGGG	2 cut(s)	256, 511
XmiI	GTMKAC	1 cut(s)	2446
XspI	CTAG	5 cut(s)	302, 644, 873, 1577, 1764

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.