Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009310

Physical Location & Seq

Genomic Coordinates Forward (+)

16444300 .. 16454223

Gene Structure 9924 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1165600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGGCCACCGCAGCCGGTGCAGCTGCTCTTTTGTATTATACGTTGAATCGTAAGTTACAGTCCCCCCTGTCGGGTGGCGATGATGTCGAAGATGGGCGTGGTGATGTGCCCAAACATGCTCCTTTGGGAATTGAACGCGTTTCGCATAGGTTAATTCAAGCCCCGGCTACATGGCTGGAGACCATTTCGACTTTGTCGGAGACGCTTAGGTTCACTTACTCCGAGACTTTGGGGAAGTGGCCTATAGGGGATTTGGCATTTGGGATTAGCTTTCTCCTAAAGAGGCAGGGAAACTTACATGTTCATGGTGTATTTGGTGGAATAGACAGTCAACAGCTAAAAGGATCTCAAACAATTGCTGAACTCAAATACCTCTTAAACTTACTGACATTGTGTTGGCATTTTTCGAAGAAACCTTTTCCATTGTTTTTAGAGGAGACAGGCTATTCAAAAGAAAATGTTCTTCTCCAGGAACCCAAAGCAGGAATCTTGAAGCCTGCTTTTACAATTCTTGTTGATCATAATAAGAAGTGTTTTCTCTTGCTGATTCGTGGAACCCATAGTATTAAGGACACTCTGACGGTTGCAACGGGAGCTGTTGTACCATTCCATCACAGTGTTGTACATGAGGGAGGAGTCAGTAATTTAGTTTTGGGTTATGCACATTGTGGAATGGTTGCAGCTGCTCGATGGATTGCAAAGCTTGCAAAACCTTGCGTACTAAAAGCACTTGAAGAATATCCTGACTATGAACTTAAGATTGTAGGGCATTCTTTGGGTGGAGGCACTGCCGCACTTCTAACATATATATTGCGGGAGCAGAAAGAACTTTCAACAACCACCTGTGTGACGTTTGCTCCAGCTGCCTGCATGACGTGGGAATTGGCAGAATCAGGAGCTGGTTTCATTACTTCTGTTATCAATGGAGCTGACCTAGTGCCCACGTTCTCTGCTGCGTCAGTGGATGATTTGCGTGCTGAGGTTACGGCATCTGCTTGGATAAATGACTTGAGAAATCAGATTGAGCAGACAAGAATTCTTAGCACTGTCTATCGATCTGCCTCAGCTTTGGGTTCACGGCTTCCCTCCATTGCCAGTGCTAAAGCAAAAGTTGCCGGGGCAGGGGCAATTCTACGCCCTGTTTCCAATGGTACACAGGTCGTTATGAGAAGAGCCCAGAGCATGGCTCAGGCAGCATGGACTCGTCCTTCCCTTGGCTTGTCATCATGGTCGTGCATGGCTCCTCGTCACCGAGCCTCGGTTGCTCATTCAAACTCAAATGATGAAGGAAGTTCTGCCGGATCCTCCGGTGGCAGCACAGAGACTTCTGACCCTCTTCTTACACCCCCCAAGAGAATCACAAGCTCAGCTGCCATTGAAACCATCGACCTTCCTGTATCTTCATCTGTAGGAATGGACTGGACTTCAGGGATTGATTATTCTTATTCTGATAGAATGCGTCAAGACACTGATGGTGAAGTCAGTGATGAGCGTGAGGGGCTCACAGACCATGATAGACATGAAGACCGTATGACAGAGGTTGAATTGTGGCAACAACTTGAGCTTGAGCTTTATGATAGAACAGAAGGTGAGGATGCCGATGTGGCAAATGAAATAAGGAATGCAGAAAATGAAATACGGGAAGAAGAAGAAGCTGCAATAGCGGAGGCAAGCGATGGTCAGCCCGAGCGCTCTGCTCCTGACATGAAGGAAGCGCATAGATTTTTTCCTGCAGGGAAAATCATGCATATTGTTACCGTTTACCATGGTGGTGCTGATAGTGAATCGAGCCACTCGGGCAATGAGCAGCCAGAAGAGACTAGGGTTGGTATTTTCCTCACTCCAAGATCTCTGTACAGTAAGCTGAGGTTGTCACAAACCATGATTAGTGATCACTTCATGCCGGTATATAGAAGACAGATTGAAAAGTTAATTAAAGAACTCGAGGAAGAAACAGCTTCCATCGGAGAAGTGGTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006638 GO:0006639 GO:0006690 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019752 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:1901568 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase3_N Lipase_3

Protein Analysis

661

Amino Acids

71.96

Weight (kDa)

5.46

Isoelectric Point (pI)

44.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase3_N	PF03893	46 - 125	2.3e-17	Lipase 3 N-terminal region
Lipase_3	PF01764	181 - 318	6.8e-29	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14075 AT3G14075 AT3G14075
fragaria_vesca	FvH4_6g35030
malus_domestica	MD09G1180000.v1.1 MD17G1165600.v1.1
prunus_persica	Prupe.3G038800_v2.0.a1 Prupe.3G038800_v2.0.a1
pyrus_communis	pycom09g09770 pycom17g15830
rosa_chinensis	RchiOBHm_Chr2g0146191
rosa_laevigata	RLG00000020185
rosa_multiflora	Rmu_sc0010454.1_g000013
rosa_roxburghii	Rroxscaffold_152G00434890 Rroxscaffold_2G00100260
rosa_rugosa	Rorug02G0393700 Rorug02G0393700
rosa_samantha	Rh2AG449400 Rh2BG461900 Rh2CG436300 Rh2DG471100
rosa_wichuraiana	Rw2G036690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1183
AccII	CGCG	1 cut(s)	138
AciI	CCGC	4 cut(s)	9, 792, 814, 1664
AclWI	GGATC	3 cut(s)	352, 1296, 1309
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	1035
AcuI	CTGAAG	1 cut(s)	1410
AdeI	CACNNNGTG	1 cut(s)	668
AfaI	GTAC	5 cut(s)	603, 624, 720, 1153, 1856
AfeI	AGCGCT	1 cut(s)	1691
AfiI	CCNNNNNNNGG	7 cut(s)	70, 71, 482, 1122, 1183, 1214, 1258
AflII	CTTAAG	1 cut(s)	755
AflIII	ACRYGT	2 cut(s)	136, 298
AjiI	CACGTC	1 cut(s)	876
AjnI	CCWGG	1 cut(s)	468
AleI	CACNNNNGTG	1 cut(s)	845
Alw26I	GTCTC	6 cut(s)	173, 194, 218, 431, 1316, 1811
AlwI	GGATC	3 cut(s)	352, 1296, 1309
AlwNI	CAGNNNCTG	1 cut(s)	899
Ama87I	CYCGRG	3 cut(s)	1685, 1795, 1943
Aor51HI	AGCGCT	1 cut(s)	1691
AoxI	GGCC	2 cut(s)	3, 239
ApoI	RAATTY	1 cut(s)	1035
Asp700I	GAANNNNTTC	1 cut(s)	459
AspLEI	GCGC	2 cut(s)	1692, 1717
AsuC2I	CCSGG	2 cut(s)	164, 1117
AsuHPI	GGTGA	4 cut(s)	113, 1241, 1487, 1601
AsuII	TTCGAA	1 cut(s)	407
AvaI	CYCGRG	3 cut(s)	1685, 1795, 1943
BaeGI	GKGCMC	2 cut(s)	111, 942
BaeI	ACNNNNGTAYC	2 cut(s)	1143, 1176
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1301
BanII	GRGCYC	2 cut(s)	1177, 1503
BbsI	GAAGAC	2 cut(s)	1530, 1921
BbvCI	CCTCAGC	3 cut(s)	978, 1063, 1865
BccI	CCATC	7 cut(s)	86, 618, 684, 1391, 1466, 1670, 1970
BceAI	ACGGC	2 cut(s)	1002, 1094
BcgI	CGANNNNNNTGC	2 cut(s)	1676, 1710
BciT130I	CCWGG	1 cut(s)	470
BclI	TGATCA	2 cut(s)	517, 1891
BcnI	CCSGG	2 cut(s)	164, 1117
BcoDI	GTCTC	6 cut(s)	173, 194, 218, 431, 1316, 1811
BfaI	CTAG	2 cut(s)	935, 1821
BfmI	CTRYAG	3 cut(s)	243, 1407, 1731
BfoI	RGCGCY	1 cut(s)	1693
BfrI	CTTAAG	1 cut(s)	755
BglI	GCCNNNNNGGC	2 cut(s)	1604, 1797
BglII	AGATCT	1 cut(s)	1847
BlpI	GCTNAGC	1 cut(s)	1366
Bme1390I	CCNGG	3 cut(s)	164, 470, 1117
BmeT110I	CYCGRG	3 cut(s)	1685, 1795, 1943
BmgBI	CACGTC	1 cut(s)	876
BmiI	GGNNCC	4 cut(s)	474, 556, 1242, 1303
BmrFI	CCNGG	3 cut(s)	164, 470, 1117
BmsI	GCATC	2 cut(s)	998, 1585
BpiI	GAAGAC	2 cut(s)	1530, 1921
BplI	GAGNNNNNCTC	2 cut(s)	1171, 1203
BpmI	CTGGAG	3 cut(s)	197, 452, 843
Bpu10I	CCTNAGC	5 cut(s)	206, 978, 1063, 1188, 1865
Bpu1102I	GCTNAGC	1 cut(s)	1366
Bpu14I	TTCGAA	1 cut(s)	407
BpuEI	CTTGAG	3 cut(s)	1030, 1580, 1586
BpuMI	CCSGG	2 cut(s)	164, 1117
Bsa29I	ATCGAT	1 cut(s)	1054
BsaI	GGTCTC	1 cut(s)	173
BsaJI	CCNNGG	5 cut(s)	162, 1116, 1213, 1257, 1765
BsaWI	WCCGGW	1 cut(s)	1307
BsaXI	ACNNNNNCTCC	5 cut(s)	585, 615, 841, 871, 1959
Bsc4I	CCNNNNNNNGG	7 cut(s)	70, 71, 482, 1122, 1183, 1214, 1258
Bse118I	RCCGGY	2 cut(s)	14, 1903
Bse1I	ACTGG	2 cut(s)	1095, 1424
Bse3DI	GCAATG	2 cut(s)	1089, 1807
BseBI	CCWGG	1 cut(s)	470
BseCI	ATCGAT	1 cut(s)	1054
BseDI	CCNNGG	5 cut(s)	162, 1116, 1213, 1257, 1765
BseGI	GGATG	2 cut(s)	970, 1600
BseLI	CCNNNNNNNGG	7 cut(s)	70, 71, 482, 1122, 1183, 1214, 1258
BseMI	GCAATG	2 cut(s)	1089, 1807
BseMII	CTCAG	5 cut(s)	969, 1077, 1202, 1380, 1856
BseNI	ACTGG	2 cut(s)	1095, 1424
BseRI	GAGGAG	3 cut(s)	449, 648, 1233
BseSI	GKGCMC	2 cut(s)	111, 942
BsgI	GTGCAG	1 cut(s)	39
Bsh1236I	CGCG	1 cut(s)	138
BshFI	GGCC	2 cut(s)	5, 241
BshVI	ATCGAT	1 cut(s)	1054
BsiHKCI	CYCGRG	3 cut(s)	1685, 1795, 1943
BsiSI	CCGG	6 cut(s)	15, 164, 1116, 1299, 1308, 1904
BslFI	GGGAC	1 cut(s)	46
BslI	CCNNNNNNNGG	7 cut(s)	70, 71, 482, 1122, 1183, 1214, 1258
BsmAI	GTCTC	6 cut(s)	173, 194, 218, 431, 1316, 1811
BsmBI	CGTCTC	1 cut(s)	194
BsmFI	GGGAC	1 cut(s)	46
BsmI	GAATGC	3 cut(s)	769, 1461, 1627
BsnI	GGCC	2 cut(s)	5, 241
Bso31I	GGTCTC	1 cut(s)	173
BsoBI	CYCGRG	3 cut(s)	1685, 1795, 1943
Bsp119I	TTCGAA	1 cut(s)	407
Bsp1286I	GDGCHC	4 cut(s)	111, 942, 1177, 1503
Bsp1407I	TGTACA	2 cut(s)	622, 1854
Bsp143I	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
Bsp1720I	GCTNAGC	1 cut(s)	1366
Bsp19I	CCATGG	1 cut(s)	1765
BspACI	CCGC	4 cut(s)	9, 792, 814, 1664
BspANI	GGCC	2 cut(s)	5, 241
BspCNI	CTCAG	5 cut(s)	970, 1076, 1201, 1379, 1857
BspDI	ATCGAT	1 cut(s)	1054
BspFNI	CGCG	1 cut(s)	138
BspLI	GGNNCC	4 cut(s)	474, 556, 1242, 1303
BspMAI	CTGCAG	1 cut(s)	1735
BspPI	GGATC	3 cut(s)	352, 1296, 1309
BspQI	GCTCTTC	1 cut(s)	1165
BspT104I	TTCGAA	1 cut(s)	407
BspTI	CTTAAG	1 cut(s)	755
BspTNI	GGTCTC	1 cut(s)	173
BsrDI	GCAATG	2 cut(s)	1089, 1807
BsrFI	RCCGGY	2 cut(s)	14, 1903
BsrGI	TGTACA	2 cut(s)	622, 1854
BsrI	ACTGG	2 cut(s)	1095, 1424
BssAI	RCCGGY	2 cut(s)	14, 1903
BssECI	CCNNGG	5 cut(s)	162, 1116, 1213, 1257, 1765
BssMI	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
BssT1I	CCWWGG	2 cut(s)	1213, 1765
Bst2UI	CCWGG	1 cut(s)	470
Bst4CI	ACNGT	8 cut(s)	60, 329, 583, 617, 1048, 1529, 1759, 1859
Bst6I	CTCTTC	3 cut(s)	1165, 1341, 1809
BstAFI	CTTAAG	1 cut(s)	755
BstAPI	GCANNNNNTGC	3 cut(s)	17, 704, 1112
BstAUI	TGTACA	2 cut(s)	622, 1854
BstBI	TTCGAA	1 cut(s)	407
BstC8I	GCNNGC	5 cut(s)	498, 705, 868, 975, 1672
BstDEI	CTNAG	7 cut(s)	206, 978, 1040, 1063, 1188, 1366, 1865
BstDSI	CCRYGG	1 cut(s)	1765
BstF5I	GGATG	2 cut(s)	970, 1600
BstFNI	CGCG	1 cut(s)	138
BstH2I	RGCGCY	1 cut(s)	1693
BstHHI	GCGC	2 cut(s)	1692, 1717
BstKTI	GATC	6 cut(s)	347, 520, 1058, 1304, 1850, 1894
BstMAI	GTCTC	6 cut(s)	173, 194, 218, 431, 1316, 1811
BstMBI	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
BstNI	CCWGG	1 cut(s)	470
BstNSI	RCATGY	2 cut(s)	119, 302
BstSCI	CCNGG	3 cut(s)	162, 468, 1115
BstSFI	CTRYAG	3 cut(s)	243, 1407, 1731
BstSLI	GKGCMC	2 cut(s)	111, 942
BstUI	CGCG	1 cut(s)	138
BstV2I	GAAGAC	2 cut(s)	1530, 1921
BstX2I	RGATCY	3 cut(s)	344, 1301, 1847
BstYI	RGATCY	3 cut(s)	344, 1301, 1847
Bsu15I	ATCGAT	1 cut(s)	1054
BsuRI	GGCC	2 cut(s)	5, 241
BsuTUI	ATCGAT	1 cut(s)	1054
BtgI	CCRYGG	1 cut(s)	1765
BtgZI	GCGATG	2 cut(s)	93, 1689
BtrI	CACGTC	1 cut(s)	876
BtsCI	GGATG	2 cut(s)	970, 1600
BtsI	GCAGTG	1 cut(s)	786
BtsIMutI	CAGTG	7 cut(s)	622, 786, 966, 1044, 1102, 1467, 1489
Cac8I	GCNNGC	5 cut(s)	498, 705, 868, 975, 1672
CaiI	CAGNNNCTG	1 cut(s)	899
CfoI	GCGC	2 cut(s)	1692, 1717
Cfr10I	RCCGGY	2 cut(s)	14, 1903
ClaI	ATCGAT	1 cut(s)	1054
CseI	GACGC	3 cut(s)	211, 945, 1448
Csp6I	GTAC	5 cut(s)	602, 623, 719, 1152, 1855
CviQI	GTAC	5 cut(s)	602, 623, 719, 1152, 1855
DdeI	CTNAG	7 cut(s)	206, 978, 1040, 1063, 1188, 1366, 1865
DpnI	GATC	6 cut(s)	346, 519, 1057, 1303, 1849, 1893
DpnII	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
DraIII	CACNNNGTG	1 cut(s)	668
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	1165, 1341, 1809
EarI	CTCTTC	3 cut(s)	1165, 1341, 1809
Eco130I	CCWWGG	2 cut(s)	1213, 1765
Eco24I	GRGCYC	2 cut(s)	1177, 1503
Eco31I	GGTCTC	1 cut(s)	173
Eco47III	AGCGCT	1 cut(s)	1691
Eco57I	CTGAAG	1 cut(s)	1410
Eco88I	CYCGRG	3 cut(s)	1685, 1795, 1943
EcoRI	GAATTC	1 cut(s)	1035
EcoRII	CCWGG	1 cut(s)	468
EcoT14I	CCWWGG	2 cut(s)	1213, 1765
EcoT22I	ATGCAT	1 cut(s)	1749
EcoT38I	GRGCYC	2 cut(s)	1177, 1503
ErhI	CCWWGG	2 cut(s)	1213, 1765
Esp3I	CGTCTC	1 cut(s)	194
FaqI	GGGAC	1 cut(s)	46
FauI	CCCGC	1 cut(s)	807
FbaI	TGATCA	2 cut(s)	517, 1891
FokI	GGATG	2 cut(s)	977, 1607
FriOI	GRGCYC	2 cut(s)	1177, 1503
FspBI	CTAG	2 cut(s)	935, 1821
GlaI	GCGC	2 cut(s)	1691, 1716
GsuI	CTGGAG	3 cut(s)	197, 452, 843
HaeII	RGCGCY	1 cut(s)	1693
HaeIII	GGCC	2 cut(s)	5, 241
HapII	CCGG	6 cut(s)	15, 164, 1116, 1299, 1308, 1904
HgaI	GACGC	3 cut(s)	211, 945, 1448
HhaI	GCGC	2 cut(s)	1692, 1717
Hin6I	GCGC	2 cut(s)	1690, 1715
HinP1I	GCGC	2 cut(s)	1690, 1715
HincII	GTYRAC	1 cut(s)	332
HindII	GTYRAC	1 cut(s)	332
HindIII	AAGCTT	1 cut(s)	701
HinfI	GANTC	8 cut(s)	46, 486, 547, 636, 890, 1201, 1356, 1784
HpaII	CCGG	6 cut(s)	15, 164, 1116, 1299, 1308, 1904
HphI	GGTGA	4 cut(s)	113, 1241, 1487, 1601
Hpy166II	GTNNAC	5 cut(s)	213, 332, 1076, 1154, 1762
Hpy188I	TCNGA	7 cut(s)	199, 223, 579, 1020, 1330, 1450, 1967
Hpy188III	TCNNGA	5 cut(s)	490, 743, 894, 1463, 1700
Hpy8I	GTNNAC	5 cut(s)	213, 332, 1076, 1154, 1762
HpyAV	CCTTC	5 cut(s)	1218, 1280, 1400, 1580, 1702
HpyCH4III	ACNGT	8 cut(s)	60, 329, 583, 617, 1048, 1529, 1759, 1859
HpyCH4IV	ACGT	4 cut(s)	41, 851, 875, 944
HpyF3I	CTNAG	7 cut(s)	206, 978, 1040, 1063, 1188, 1366, 1865
HpySE526I	ACGT	4 cut(s)	41, 851, 875, 944
HspAI	GCGC	2 cut(s)	1690, 1715
Ksp22I	TGATCA	2 cut(s)	517, 1891
Kzo9I	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
LguI	GCTCTTC	1 cut(s)	1165
LmnI	GCTCC	8 cut(s)	124, 593, 817, 862, 896, 926, 1246, 1702
LweI	GCATC	2 cut(s)	998, 1585
MaeI	CTAG	2 cut(s)	935, 1821
MaeII	ACGT	4 cut(s)	41, 851, 875, 944
MaeIII	GTNAC	6 cut(s)	54, 847, 982, 1247, 1753, 1872
MalI	GATC	6 cut(s)	346, 519, 1057, 1303, 1849, 1893
MboI	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
MfeI	CAATTG	1 cut(s)	354
MflI	RGATCY	3 cut(s)	344, 1301, 1847
MhlI	GDGCHC	4 cut(s)	111, 942, 1177, 1503
MlsI	TGGCCA	1 cut(s)	5
MluI	ACGCGT	1 cut(s)	136
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	645, 1195
MmeI	TCCRAC	1 cut(s)	177
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	1749
MroXI	GAANNNNTTC	1 cut(s)	459
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	152, 377, 567, 756, 1931, 1935
MslI	CAYNNNNRTG	5 cut(s)	303, 615, 845, 1231, 1770
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	4 cut(s)	23, 683, 863, 1370
MspCI	CTTAAG	1 cut(s)	755
MspI	CCGG	6 cut(s)	15, 164, 1116, 1299, 1308, 1904
MspR9I	CCNGG	3 cut(s)	164, 470, 1117
MunI	CAATTG	1 cut(s)	354
Mva1269I	GAATGC	3 cut(s)	769, 1461, 1627
MvaI	CCWGG	1 cut(s)	470
MvnI	CGCG	1 cut(s)	138
NciI	CCSGG	2 cut(s)	164, 1117
NcoI	CCATGG	1 cut(s)	1765
NdeII	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
NlaIV	GGNNCC	4 cut(s)	474, 556, 1242, 1303
NmuCI	GTSAC	3 cut(s)	847, 1247, 1872
NsiI	ATGCAT	1 cut(s)	1749
NspI	RCATGY	2 cut(s)	119, 302
NspV	TTCGAA	1 cut(s)	407
OliI	CACNNNNGTG	1 cut(s)	845
PacI	TTAATTAA	1 cut(s)	1935
PaeR7I	CTCGAG	1 cut(s)	1943
PciI	ACATGT	1 cut(s)	298
PciSI	GCTCTTC	1 cut(s)	1165
PctI	GAATGC	3 cut(s)	769, 1461, 1627
PdmI	GAANNNNTTC	1 cut(s)	459
PfeI	GAWTC	6 cut(s)	46, 486, 547, 890, 1356, 1784
PflFI	GACNNNGTC	1 cut(s)	193
PflMI	CCANNNNNTGG	1 cut(s)	1183
PfoI	TCCNGGA	1 cut(s)	468
PleI	GAGTC	2 cut(s)	644, 1195
PpsI	GAGTC	2 cut(s)	644, 1195
PscI	ACATGT	1 cut(s)	298
Psp6I	CCWGG	1 cut(s)	468
PspGI	CCWGG	1 cut(s)	468
PspN4I	GGNNCC	4 cut(s)	474, 556, 1242, 1303
PspXI	VCTCGAGB	1 cut(s)	1943
PstI	CTGCAG	1 cut(s)	1735
PstNI	CAGNNNCTG	1 cut(s)	899
PsuI	RGATCY	3 cut(s)	344, 1301, 1847
PsyI	GACNNNGTC	1 cut(s)	193
PvuII	CAGCTG	4 cut(s)	23, 683, 863, 1370
RsaI	GTAC	5 cut(s)	603, 624, 720, 1153, 1856
RsaNI	GTAC	5 cut(s)	602, 623, 719, 1152, 1855
RseI	CAYNNNNRTG	5 cut(s)	303, 615, 845, 1231, 1770
SapI	GCTCTTC	1 cut(s)	1165
SaqAI	TTAA	6 cut(s)	152, 377, 567, 756, 1931, 1935
Sau3AI	GATC	6 cut(s)	344, 517, 1055, 1301, 1847, 1891
SbfI	CCTGCAGG	1 cut(s)	1735
SchI	GAGTC	2 cut(s)	645, 1195
ScrFI	CCNGG	3 cut(s)	164, 470, 1117
SdaI	CCTGCAGG	1 cut(s)	1735
SduI	GDGCHC	4 cut(s)	111, 942, 1177, 1503
SfaNI	GCATC	2 cut(s)	998, 1585
SfcI	CTRYAG	3 cut(s)	243, 1407, 1731
Sfr274I	CTCGAG	1 cut(s)	1943
SfuI	TTCGAA	1 cut(s)	407
SlaI	CTCGAG	1 cut(s)	1943
SmiMI	CAYNNNNRTG	5 cut(s)	303, 615, 845, 1231, 1770
SmlI	CTYRAG	5 cut(s)	755, 1009, 1559, 1565, 1943
SmoI	CTYRAG	5 cut(s)	755, 1009, 1559, 1565, 1943
Sse8387I	CCTGCAGG	1 cut(s)	1735
SsiI	CCGC	4 cut(s)	9, 792, 814, 1664
SspMI	CTAG	2 cut(s)	935, 1821
StyD4I	CCNGG	3 cut(s)	162, 468, 1115
StyI	CCWWGG	2 cut(s)	1213, 1765
TaaI	ACNGT	8 cut(s)	60, 329, 583, 617, 1048, 1529, 1759, 1859
TaiI	ACGT	4 cut(s)	44, 854, 878, 947
TaqI	TCGA	8 cut(s)	87, 188, 407, 688, 1054, 1386, 1787, 1944
TatI	WGTACW	2 cut(s)	622, 1854
TauI	GCSGC	1 cut(s)	794
TfiI	GAWTC	6 cut(s)	46, 486, 547, 890, 1356, 1784
Tru1I	TTAA	6 cut(s)	152, 377, 567, 756, 1931, 1935
Tru9I	TTAA	6 cut(s)	152, 377, 567, 756, 1931, 1935
TscAI	CASTG	7 cut(s)	622, 793, 966, 1051, 1102, 1474, 1489
TseFI	GTSAC	3 cut(s)	847, 1247, 1872
Tsp45I	GTSAC	3 cut(s)	847, 1247, 1872
TspRI	CASTG	7 cut(s)	622, 793, 966, 1051, 1102, 1474, 1489
Tth111I	GACNNNGTC	1 cut(s)	193
Van91I	CCANNNNNTGG	1 cut(s)	1183
Vha464I	CTTAAG	1 cut(s)	755
XapI	RAATTY	1 cut(s)	1035
XceI	RCATGY	2 cut(s)	119, 302
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1969
XhoI	CTCGAG	1 cut(s)	1943
XmnI	GAANNNNTTC	1 cut(s)	459
XspI	CTAG	2 cut(s)	935, 1821
Zsp2I	ATGCAT	1 cut(s)	1749

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.