Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0009310

Physical Location & Seq

Genomic Coordinates Reverse (-)

65349845 .. 65355878

Gene Structure 6034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG461900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGGCAACTGCAACTATGGCCACCGCAGCCGGTGCAGCTGCTCTCTTGTATTACACATTGAACCGCAAGTTACAGACCGGCGGTGATGATGACGAAAGAGGTGGTGGCGGCGGCTTGACCGATCAGGCGACTTTGGGAATCGACCGCGTTTCCAATAGGTTAATTCAGGCCCCTGCTACCTGGTTGGAGACTATTTCCACCTTGTCTGAGACTCTTAGGTTTACTTACTCTGAGACTCTCGGCAAGTGGCCTATCGGGGACTTGGCTTTCGGAATCAGCTTCCTTCTCAAGAGGCAGGGAAACTTACTTGTTGATGGTGTATTTGGTGGAGAAGAAAGTCAACAGCTAAAGGGGCCTGAAATAGTAGAGGAACTTAAGTACCTCTTAAACTTATTAACACTGTGTTGGCACTTTTCCAAGAAACCTTTTCCTTTATTTTTAGAGGAGACGGGGTATTCTGAAGAGAATGTTCTTCTTCAGGAACCCAAAGCAGGAATTTTGAAGCCTGCGTTTACAATTCTGGTCGATCATAACCAAAAATGCTTTCTCTTGTTGATTCGTGGAACCCATAGTATCAAGGACACTTTGACAGCGGCAACAGGGGCTGTTGTACCTTTCCATCACAGTGTTGTGCATGAGGGAGGGGTCAGTGATCTAGTTTTGGGTTATGCACATTGTGGAATGGTCGCGGCTGCAAGGTGGATTGCAAAGCTTGCAAAACCGCGTCTTCTAGAAGCGCTTGACCAGTATCCTGGCTATGAACTTAAGATTGTAGGGCATTCTTTGGGTGGAGGCACTGCAGCACTTTTAACATATATATTGCGTGAGCAGAAAGAACTTGCAACAACCACTTGTGTGACGTTTGCTCCAGCTGCCTGTATGACATGGGAATTGGCAGAATCAGGCGCTAAGTTTATTACTTCCGTTGTTAATGGTGCTGACTTAGTGCCCACCTTCTCTGCTGCATCAGTGGATGATTTACGTGCTGAGGTAACTGCATCTGCCTGGCTAAATGATCTGAGAAATCAGATAGAGCAGACAAGAATCCTTAGCACTGTCTATCGTTCTGCTTCAGCTTTGGGTTCACGCCTTCCATCCATTGCGACTGCGAAAGCAACGGTCGCAGGGGCAGGGGCGATTCTGCGCCCTGTTTCCAGTGGTACACAGGTTGTTATGAGAAGAGCTCAGAGCATGGCAGCATGGGCACGTCCTAACCTTCGCTTATCATCATGGAACTGCATGGCTCCTCGTCACAGAACCTCAGCTGTCTGTTCAAATTCAAATGATGAGGCAAGTTCTGCAGAGTCCTCATGTGGCCACACTGAGACTTCTGAGCCTCTCCTTACGTCTCCTAAGACAAGGACGAGCTCAAGTGTCATTGAAAGCCTTGACCTTCCTGTATCTACATCTGTAAGAATGGAGTGGACTTCAGAGATTGGATGCTCTATTTCTGATAGTGGGATGCGTCCAGATATTGATGAGGATGTCAGTAATGAAGGTGACCATGATAGACATGAAGATCGCATGACTGAAGCTGAATTGTGGCAACAACTTGAGCATGAACTTTATGATAGAACAGAAGGTGAGGAGGCAGATGTAGCAAAAGAAATAAGGGAAGAAGAAGAAGAGGCAAGAAGGGAAGAAGTAAACGAAGCGTCAATAAGGGAAGAAGAAGAAGCGGCAATTGCAGAGGTAAGCAATGGCCAGCAGGAGAGCTCTTCTCCAGGAATGAAGGAAGCGCACAGATTTTTTCCTGCAGGCAAAATCATGCATATTGTTACCCTTCATAGTGGTGCTACTAATTGTGAAAGTGGCAGGCCTAGCTCCAGTGGCTCGGATGAGCAGCAGCAGCCAGAAGAGACTAGGATTGGTATTTTCCTCACTCCAAGATCTCTGTACAGTAAGCTGAGGTTGTCGCAAACAATGATCAGTGATCACTTCATGCCAGTCTATAGAAGACGGATTGAGAAGCTGATAAAAGAATTTGAGAAAGAAGCAGCTTCCATCGGAGAGGTAGTATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006638 GO:0006639 GO:0006690 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019752 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:1901568 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase3_N Lipase_3

Protein Analysis

674

Amino Acids

73.62

Weight (kDa)

5.14

Isoelectric Point (pI)

49.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase3_N	PF03893	49 - 128	1.2e-18	Lipase 3 N-terminal region
Lipase_3	PF01764	184 - 321	3.2e-29	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14075 AT3G14075 AT3G14075
fragaria_vesca	FvH4_6g35030
malus_domestica	MD09G1180000.v1.1 MD17G1165600.v1.1
prunus_persica	Prupe.3G038800_v2.0.a1 Prupe.3G038800_v2.0.a1
pyrus_communis	pycom09g09770 pycom17g15830
rosa_chinensis	RchiOBHm_Chr2g0146191
rosa_laevigata	RLG00000020185
rosa_multiflora	Rmu_sc0010454.1_g000013
rosa_roxburghii	Rroxscaffold_152G00434890 Rroxscaffold_2G00100260
rosa_rugosa	Rorug02G0393700 Rorug02G0393700
rosa_samantha	Rh2AG449400 Rh2BG461900 Rh2CG436300 Rh2DG471100
rosa_wichuraiana	Rw2G036690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	147, 689, 724
AcoI	YGGCCR	3 cut(s)	18, 1315, 1702
AcsI	RAATTY	3 cut(s)	495, 1276, 1982
AcuI	CTGAAG	5 cut(s)	461, 480, 1056, 1413, 1551
AdeI	CACNNNGTG	1 cut(s)	677
AfaI	GTAC	4 cut(s)	380, 612, 1162, 1898
AfeI	AGCGCT	1 cut(s)	738
AfiI	CCNNNNNNNGG	1 cut(s)	491
AflII	CTTAAG	2 cut(s)	374, 764
AgsI	TTSAA	5 cut(s)	61, 502, 1275, 1281, 1382
AjiI	CACGTC	1 cut(s)	1208
AjnI	CCWGG	4 cut(s)	179, 751, 1004, 1723
AjuI	GAANNNNNNNTTGG	2 cut(s)	528, 560
AleI	CACNNNNGTG	1 cut(s)	854
Alw21I	GWGCWC	3 cut(s)	1186, 1370, 1718
Alw26I	GTCTC	7 cut(s)	182, 203, 227, 440, 1319, 1353, 1853
AlwNI	CAGNNNCTG	1 cut(s)	605
Aor51HI	AGCGCT	1 cut(s)	738
AoxI	GGCC	7 cut(s)	18, 168, 248, 353, 1315, 1702, 1817
ApoI	RAATTY	3 cut(s)	495, 1276, 1982
ArsI	GACNNNNNNTTYG	2 cut(s)	251, 283
AspLEI	GCGC	4 cut(s)	739, 908, 1146, 1741
AspS9I	GGNCC	2 cut(s)	169, 353
AsuHPI	GGTGA	3 cut(s)	95, 1511, 1595
BaeGI	GKGCMC	2 cut(s)	951, 1207
BaeI	ACNNNNGTAYC	4 cut(s)	362, 395, 1152, 1185
BalI	TGGCCA	3 cut(s)	20, 1317, 1704
BanII	GRGCYC	3 cut(s)	1186, 1370, 1718
BbsI	GAAGAC	2 cut(s)	719, 1963
Bbv12I	GWGCWC	3 cut(s)	1186, 1370, 1718
BbvCI	CCTCAGC	3 cut(s)	987, 1261, 1907
BccI	CCATC	4 cut(s)	308, 627, 1102, 2012
BciT130I	CCWGG	4 cut(s)	181, 753, 1006, 1725
BciVI	GTATCC	1 cut(s)	759
BclI	TGATCA	2 cut(s)	1926, 1933
BcoDI	GTCTC	7 cut(s)	182, 203, 227, 440, 1319, 1353, 1853
BfaI	CTAG	4 cut(s)	656, 731, 1821, 1863
BfmI	CTRYAG	4 cut(s)	798, 1299, 1755, 1951
BfoI	RGCGCY	2 cut(s)	740, 909
BfrI	CTTAAG	2 cut(s)	374, 764
BfuI	GTATCC	1 cut(s)	759
BglII	AGATCT	1 cut(s)	1889
Bme1390I	CCNGG	4 cut(s)	181, 753, 1006, 1725
BmgBI	CACGTC	1 cut(s)	1208
BmgT120I	GGNCC	2 cut(s)	169, 353
BmiI	GGNNCC	5 cut(s)	171, 354, 483, 565, 1245
BmrFI	CCNGG	4 cut(s)	181, 753, 1006, 1725
BmsI	GCATC	4 cut(s)	974, 1007, 1430, 1452
BpiI	GAAGAC	2 cut(s)	719, 1963
BplI	GAGNNNNNCTC	4 cut(s)	1168, 1200, 1705, 1737
BpmI	CTGGAG	3 cut(s)	852, 1707, 1810
Bpu10I	CCTNAGC	4 cut(s)	987, 1049, 1261, 1907
BpuEI	CTTGAG	3 cut(s)	272, 1354, 1574
BsaAI	YACGTR	1 cut(s)	983
BsaXI	ACNNNNNCTCC	5 cut(s)	437, 467, 850, 880, 2001
Bsc4I	CCNNNNNNNGG	1 cut(s)	491
Bse118I	RCCGGY	2 cut(s)	29, 77
Bse1I	ACTGG	4 cut(s)	745, 1155, 1827, 1946
Bse3DI	GCAATG	2 cut(s)	1098, 1705
BseBI	CCWGG	4 cut(s)	181, 753, 1006, 1725
BseGI	GGATG	6 cut(s)	979, 1094, 1445, 1467, 1489, 1843
BseLI	CCNNNNNNNGG	1 cut(s)	491
BseMI	GCAATG	2 cut(s)	1098, 1705
BseMII	CTCAG	9 cut(s)	198, 222, 978, 1010, 1199, 1275, 1314, 1323, 1898
BseNI	ACTGG	4 cut(s)	745, 1155, 1827, 1946
BseRI	GAGGAG	3 cut(s)	458, 1236, 1601
BseSI	GKGCMC	2 cut(s)	951, 1207
BsgI	GTGCAG	1 cut(s)	54
Bsh1236I	CGCG	3 cut(s)	147, 689, 724
Bsh1285I	CGRYCG	2 cut(s)	145, 1122
BshFI	GGCC	7 cut(s)	20, 170, 250, 355, 1317, 1704, 1819
BsiEI	CGRYCG	2 cut(s)	145, 1122
BsiHKAI	GWGCWC	3 cut(s)	1186, 1370, 1718
BsiSI	CCGG	2 cut(s)	30, 78
BslFI	GGGAC	1 cut(s)	272
BslI	CCNNNNNNNGG	1 cut(s)	491
BsmAI	GTCTC	7 cut(s)	182, 203, 227, 440, 1319, 1353, 1853
BsmBI	CGTCTC	2 cut(s)	440, 1353
BsmFI	GGGAC	1 cut(s)	272
BsmI	GAATGC	1 cut(s)	778
BsnI	GGCC	7 cut(s)	20, 170, 250, 355, 1317, 1704, 1819
Bsp1286I	GDGCHC	5 cut(s)	951, 1186, 1207, 1370, 1718
Bsp1407I	TGTACA	1 cut(s)	1896
Bsp143I	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
BspANI	GGCC	7 cut(s)	20, 170, 250, 355, 1317, 1704, 1819
BspCNI	CTCAG	9 cut(s)	199, 223, 979, 1011, 1198, 1274, 1315, 1324, 1899
BspFNI	CGCG	3 cut(s)	147, 689, 724
BspLI	GGNNCC	5 cut(s)	171, 354, 483, 565, 1245
BspMAI	CTGCAG	3 cut(s)	802, 1303, 1759
BspQI	GCTCTTC	2 cut(s)	1174, 1723
BspTI	CTTAAG	2 cut(s)	374, 764
BsrDI	GCAATG	2 cut(s)	1098, 1705
BsrFI	RCCGGY	2 cut(s)	29, 77
BsrGI	TGTACA	1 cut(s)	1896
BsrI	ACTGG	4 cut(s)	745, 1155, 1827, 1946
BssAI	RCCGGY	2 cut(s)	29, 77
BssMI	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
Bst2UI	CCWGG	4 cut(s)	181, 753, 1006, 1725
Bst4CI	ACNGT	5 cut(s)	402, 626, 1057, 1120, 1901
Bst6I	CTCTTC	5 cut(s)	456, 1174, 1620, 1723, 1851
BstAFI	CTTAAG	2 cut(s)	374, 764
BstAPI	GCANNNNNTGC	3 cut(s)	32, 713, 1298
BstAUI	TGTACA	1 cut(s)	1896
BstBAI	YACGTR	1 cut(s)	983
BstC8I	GCNNGC	5 cut(s)	507, 714, 1706, 1759, 1817
BstEII	GGTNACC	1 cut(s)	1499
BstF5I	GGATG	6 cut(s)	979, 1094, 1445, 1467, 1489, 1843
BstFNI	CGCG	3 cut(s)	147, 689, 724
BstH2I	RGCGCY	2 cut(s)	740, 909
BstHHI	GCGC	4 cut(s)	739, 908, 1146, 1741
BstKTI	GATC	8 cut(s)	124, 529, 655, 1018, 1522, 1892, 1929, 1936
BstMAI	GTCTC	7 cut(s)	182, 203, 227, 440, 1319, 1353, 1853
BstMBI	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
BstMCI	CGRYCG	2 cut(s)	145, 1122
BstNI	CCWGG	4 cut(s)	181, 753, 1006, 1725
BstPI	GGTNACC	1 cut(s)	1499
BstSCI	CCNGG	4 cut(s)	179, 751, 1004, 1723
BstSFI	CTRYAG	4 cut(s)	798, 1299, 1755, 1951
BstSLI	GKGCMC	2 cut(s)	951, 1207
BstUI	CGCG	3 cut(s)	147, 689, 724
BstV2I	GAAGAC	2 cut(s)	719, 1963
BstX2I	RGATCY	1 cut(s)	1889
BstXI	CCANNNNNNTGG	1 cut(s)	752
BstYI	RGATCY	1 cut(s)	1889
BsuI	GTATCC	1 cut(s)	759
BsuRI	GGCC	7 cut(s)	20, 170, 250, 355, 1317, 1704, 1819
BtrI	CACGTC	1 cut(s)	1208
BtsCI	GGATG	6 cut(s)	979, 1094, 1445, 1467, 1489, 1843
BtsI	GCAGTG	1 cut(s)	795
Cac8I	GCNNGC	5 cut(s)	507, 714, 1706, 1759, 1817
CaiI	CAGNNNCTG	1 cut(s)	605
CfoI	GCGC	4 cut(s)	739, 908, 1146, 1741
Cfr10I	RCCGGY	2 cut(s)	29, 77
Cfr13I	GGNCC	2 cut(s)	169, 353
CseI	GACGC	3 cut(s)	713, 1454, 1644
CsiI	ACCWGGT	1 cut(s)	179
Csp6I	GTAC	4 cut(s)	379, 611, 1161, 1897
CviQI	GTAC	4 cut(s)	379, 611, 1161, 1897
DpnI	GATC	8 cut(s)	123, 528, 654, 1017, 1521, 1891, 1928, 1935
DpnII	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
DraIII	CACNNNGTG	1 cut(s)	677
EaeI	YGGCCR	3 cut(s)	18, 1315, 1702
Eam1104I	CTCTTC	5 cut(s)	456, 1174, 1620, 1723, 1851
EarI	CTCTTC	5 cut(s)	456, 1174, 1620, 1723, 1851
Ecl136II	GAGCTC	3 cut(s)	1184, 1368, 1716
Eco147I	AGGCCT	1 cut(s)	1819
Eco24I	GRGCYC	3 cut(s)	1186, 1370, 1718
Eco47III	AGCGCT	1 cut(s)	738
Eco53kI	GAGCTC	3 cut(s)	1184, 1368, 1716
Eco57I	CTGAAG	5 cut(s)	461, 480, 1056, 1413, 1551
Eco91I	GGTNACC	1 cut(s)	1499
EcoICRI	GAGCTC	3 cut(s)	1184, 1368, 1716
EcoO109I	RGGNCCY	2 cut(s)	169, 353
EcoO65I	GGTNACC	1 cut(s)	1499
EcoRII	CCWGG	4 cut(s)	179, 751, 1004, 1723
EcoT22I	ATGCAT	1 cut(s)	1773
EcoT38I	GRGCYC	3 cut(s)	1186, 1370, 1718
Esp3I	CGTCTC	2 cut(s)	440, 1353
FalI	AAGNNNNNCTT	2 cut(s)	368, 400
FaqI	GGGAC	1 cut(s)	272
FbaI	TGATCA	2 cut(s)	1926, 1933
FokI	GGATG	6 cut(s)	986, 1081, 1452, 1474, 1496, 1850
FriOI	GRGCYC	3 cut(s)	1186, 1370, 1718
FspBI	CTAG	4 cut(s)	656, 731, 1821, 1863
GlaI	GCGC	4 cut(s)	738, 907, 1145, 1740
GsuI	CTGGAG	3 cut(s)	852, 1707, 1810
HaeII	RGCGCY	2 cut(s)	740, 909
HaeIII	GGCC	7 cut(s)	20, 170, 250, 355, 1317, 1704, 1819
HapII	CCGG	2 cut(s)	30, 78
HgaI	GACGC	3 cut(s)	713, 1454, 1644
HhaI	GCGC	4 cut(s)	739, 908, 1146, 1741
Hin6I	GCGC	4 cut(s)	737, 906, 1144, 1739
HinP1I	GCGC	4 cut(s)	737, 906, 1144, 1739
HincII	GTYRAC	1 cut(s)	341
HindII	GTYRAC	1 cut(s)	341
HindIII	AAGCTT	1 cut(s)	710
HinfI	GANTC	9 cut(s)	138, 211, 235, 273, 556, 899, 1044, 1138, 1304
HpaII	CCGG	2 cut(s)	30, 78
HphI	GGTGA	3 cut(s)	95, 1511, 1595
Hpy166II	GTNNAC	7 cut(s)	222, 341, 513, 1085, 1163, 1425, 1648
Hpy188III	TCNNGA	4 cut(s)	289, 479, 731, 1469
Hpy8I	GTNNAC	7 cut(s)	222, 341, 513, 1085, 1163, 1425, 1648
HpyCH4III	ACNGT	5 cut(s)	402, 626, 1057, 1120, 1901
HpyCH4IV	ACGT	4 cut(s)	860, 982, 1207, 1346
HpySE526I	ACGT	4 cut(s)	860, 982, 1207, 1346
HspAI	GCGC	4 cut(s)	737, 906, 1144, 1739
Ksp22I	TGATCA	2 cut(s)	1926, 1933
Kzo9I	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
LguI	GCTCTTC	2 cut(s)	1174, 1723
LmnI	GCTCC	3 cut(s)	871, 1249, 1829
LweI	GCATC	4 cut(s)	974, 1007, 1430, 1452
MabI	ACCWGGT	1 cut(s)	179
MaeI	CTAG	4 cut(s)	656, 731, 1821, 1863
MaeII	ACGT	4 cut(s)	860, 982, 1207, 1346
MaeIII	GTNAC	6 cut(s)	69, 856, 991, 1250, 1499, 1777
MalI	GATC	8 cut(s)	123, 528, 654, 1017, 1521, 1891, 1928, 1935
MboI	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
MfeI	CAATTG	1 cut(s)	1683
MflI	RGATCY	1 cut(s)	1889
MhlI	GDGCHC	5 cut(s)	951, 1186, 1207, 1370, 1718
MlsI	TGGCCA	3 cut(s)	20, 1317, 1704
MluCI	AATT	9 cut(s)	162, 495, 516, 890, 1276, 1538, 1683, 1801, 1982
MluNI	TGGCCA	3 cut(s)	20, 1317, 1704
MlyI	GAGTC	3 cut(s)	205, 229, 1313
MmeI	TCCRAC	1 cut(s)	165
Mox20I	TGGCCA	3 cut(s)	20, 1317, 1704
Mph1103I	ATGCAT	1 cut(s)	1773
MscI	TGGCCA	3 cut(s)	20, 1317, 1704
MseI	TTAA	7 cut(s)	161, 375, 386, 395, 765, 809, 930
MslI	CAYNNNNRTG	3 cut(s)	624, 854, 1791
Msp20I	TGGCCA	3 cut(s)	20, 1317, 1704
MspA1I	CMGCKG	4 cut(s)	38, 593, 872, 1265
MspCI	CTTAAG	2 cut(s)	374, 764
MspI	CCGG	2 cut(s)	30, 78
MspR9I	CCNGG	4 cut(s)	181, 753, 1006, 1725
MunI	CAATTG	1 cut(s)	1683
Mva1269I	GAATGC	1 cut(s)	778
MvaI	CCWGG	4 cut(s)	181, 753, 1006, 1725
MvnI	CGCG	3 cut(s)	147, 689, 724
NdeII	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
NlaIV	GGNNCC	5 cut(s)	171, 354, 483, 565, 1245
NmeAIII	GCCGAG	1 cut(s)	219
NmuCI	GTSAC	3 cut(s)	856, 1250, 1499
NsiI	ATGCAT	1 cut(s)	1773
OliI	CACNNNNGTG	1 cut(s)	854
PceI	AGGCCT	1 cut(s)	1819
PciSI	GCTCTTC	2 cut(s)	1174, 1723
PctI	GAATGC	1 cut(s)	778
PfeI	GAWTC	6 cut(s)	138, 273, 556, 899, 1044, 1138
PfoI	TCCNGGA	1 cut(s)	1723
PleI	GAGTC	3 cut(s)	205, 229, 1312
PpsI	GAGTC	3 cut(s)	205, 229, 1312
Ppu21I	YACGTR	1 cut(s)	983
Psp124BI	GAGCTC	3 cut(s)	1186, 1370, 1718
Psp6I	CCWGG	4 cut(s)	179, 751, 1004, 1723
PspEI	GGTNACC	1 cut(s)	1499
PspGI	CCWGG	4 cut(s)	179, 751, 1004, 1723
PspN4I	GGNNCC	5 cut(s)	171, 354, 483, 565, 1245
PspPI	GGNCC	2 cut(s)	169, 353
PstI	CTGCAG	3 cut(s)	802, 1303, 1759
PstNI	CAGNNNCTG	1 cut(s)	605
PsuI	RGATCY	1 cut(s)	1889
PvuII	CAGCTG	3 cut(s)	38, 872, 1265
RsaI	GTAC	4 cut(s)	380, 612, 1162, 1898
RsaNI	GTAC	4 cut(s)	379, 611, 1161, 1897
RseI	CAYNNNNRTG	3 cut(s)	624, 854, 1791
SacI	GAGCTC	3 cut(s)	1186, 1370, 1718
SapI	GCTCTTC	2 cut(s)	1174, 1723
SaqAI	TTAA	7 cut(s)	161, 375, 386, 395, 765, 809, 930
Sau3AI	GATC	8 cut(s)	121, 526, 652, 1015, 1519, 1889, 1926, 1933
Sau96I	GGNCC	2 cut(s)	169, 353
SbfI	CCTGCAGG	1 cut(s)	1759
SchI	GAGTC	3 cut(s)	205, 229, 1313
ScrFI	CCNGG	4 cut(s)	181, 753, 1006, 1725
SdaI	CCTGCAGG	1 cut(s)	1759
SduI	GDGCHC	5 cut(s)	951, 1186, 1207, 1370, 1718
SexAI	ACCWGGT	1 cut(s)	179
SfaNI	GCATC	4 cut(s)	974, 1007, 1430, 1452
SfcI	CTRYAG	4 cut(s)	798, 1299, 1755, 1951
SmiMI	CAYNNNNRTG	3 cut(s)	624, 854, 1791
SmlI	CTYRAG	5 cut(s)	287, 374, 764, 1369, 1553
SmoI	CTYRAG	5 cut(s)	287, 374, 764, 1369, 1553
Sse8387I	CCTGCAGG	1 cut(s)	1759
Sse9I	AATT	9 cut(s)	162, 495, 516, 890, 1276, 1538, 1683, 1801, 1982
SseBI	AGGCCT	1 cut(s)	1819
SspMI	CTAG	4 cut(s)	656, 731, 1821, 1863
SstI	GAGCTC	3 cut(s)	1186, 1370, 1718
StuI	AGGCCT	1 cut(s)	1819
StyD4I	CCNGG	4 cut(s)	179, 751, 1004, 1723
TaaI	ACNGT	5 cut(s)	402, 626, 1057, 1120, 1901
TaiI	ACGT	4 cut(s)	863, 985, 1210, 1349
TaqI	TCGA	2 cut(s)	141, 525
TaqII	GACCGA	1 cut(s)	134
TasI	AATT	9 cut(s)	162, 495, 516, 890, 1276, 1538, 1683, 1801, 1982
TatI	WGTACW	1 cut(s)	1896
TauI	GCSGC	5 cut(s)	111, 114, 596, 692, 1682
TfiI	GAWTC	6 cut(s)	138, 273, 556, 899, 1044, 1138
Tru1I	TTAA	7 cut(s)	161, 375, 386, 395, 765, 809, 930
Tru9I	TTAA	7 cut(s)	161, 375, 386, 395, 765, 809, 930
TseFI	GTSAC	3 cut(s)	856, 1250, 1499
Tsp45I	GTSAC	3 cut(s)	856, 1250, 1499
TspDTI	ATGAA	7 cut(s)	774, 1509, 1530, 1575, 1745, 1775, 1930
TspGWI	ACGGA	2 cut(s)	913, 1975
Vha464I	CTTAAG	2 cut(s)	374, 764
XapI	RAATTY	3 cut(s)	495, 1276, 1982
XbaI	TCTAGA	1 cut(s)	730
XspI	CTAG	4 cut(s)	656, 731, 1821, 1863
Zsp2I	ATGCAT	1 cut(s)	1773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.