MD17G1207700.v1.1
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012767
Physical Location & Seq
Reverse (-)
25042309 .. 25044153
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1207700.v1.1.491

Sequence Viewer

Length: 519 bp
ATGAAGGAAAGCATGGCAAACCCTCTACATCTGAAATCTTTGAACCACATCTCCCTTATCTGCCGATCAGTTGAGGAATCAATAAATTTTTACCAGAATGTCCTCGGCTTTGTACCAATTAGGAGGCCTGGCTCCTTTGATTTTGATGGGGCATGGCTATTTGGGTATGGAATTGGAATACATCTTTTGCAATCTGAGGATCCGGAGAACATGCCCAAGAAAACTGAGATTAATCCCAAGGATAATCATATTTCTTTTCAGTGTGAGAGCATGGGAGCCGTTGAGAAGAAGCTGAAGGAGATGGAACTGAAATACAAGCGATCCATGGTGGAAGAAGGTGGCATTCACGTTGATCAGTTGTTCTTCCATGACCCGGATGGCTTCATGATCGAAATATGTGACTGCGACAACCTCCCCGTGGTTCCTATCGCCGGCGGGATGGCTCGGGCATGTTCCCTTGTAAACCTGCCTAGGTTGCAGCAACCACAGCAGCCGCAGATAAGAGTTATTCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

173

Amino Acids

19.54

Weight (kDa)

5.66

Isoelectric Point (pI)

69.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 14 - 132 5e-13 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15380 AT1G15380 AT1G80160 AT1G80160
fragaria_vesca FvH4_6g30160
malus_domestica MD17G1207700.v1.1
prunus_persica Prupe.3G082100_v2.0.a1
pyrus_communis pycom17g21020
rosa_chinensis RchiOBHm_Chr3g0458691
rosa_laevigata RLG00000019525
rosa_multiflora Rmu_sc0001998.1_g000022
rosa_roxburghii Rroxscaffold_2G00108340
rosa_rugosa Rorug02G0329400
rosa_samantha Rh2AG380900 Rh2BG374500 Rh2BG387700 Rh2DG404000
rosa_wichuraiana Rw2G031150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 474
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 2 cut(s) 435, 494
AclWI GGATC 3 cut(s) 194, 207, 315
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 373, 418, 431
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 127
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
AluBI AGCT 1 cut(s) 292
AluI AGCT 1 cut(s) 292
AlwI GGATC 3 cut(s) 194, 207, 315
Ama87I CYCGRG 1 cut(s) 444
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 478, 490
ApoI RAATTY 1 cut(s) 85
AseI ATTAAT 1 cut(s) 231
AspA2I CCTAGG 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 444
AvrII CCTAGG 1 cut(s) 470
BamHI GGATCC 1 cut(s) 199
BbvI GCAGC 2 cut(s) 490, 502
BccI CCATC 4 cut(s) 140, 295, 371, 433
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 129
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 1 cut(s) 374
BfaI CTAG 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 474
BisI GCNGC 3 cut(s) 479, 491, 494
BlnI CCTAGG 1 cut(s) 470
BlsI GCNGC 3 cut(s) 480, 492, 495
Bme1390I CCNGG 2 cut(s) 129, 374
BmeT110I CYCGRG 1 cut(s) 444
BmiI GGNNCC 4 cut(s) 133, 201, 277, 423
BmrFI CCNGG 2 cut(s) 129, 374
BpuMI CCSGG 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 103, 237, 324, 417, 470
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 418, 431
Bse118I RCCGGY 1 cut(s) 431
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 5 cut(s) 103, 237, 324, 417, 470
BseGI GGATG 2 cut(s) 382, 444
BseLI CCNNNNNNNGG 3 cut(s) 373, 418, 431
BseMII CTCAG 2 cut(s) 186, 216
BseXI GCAGC 2 cut(s) 490, 502
BshFI GGCC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 3 cut(s) 203, 374, 432
BslI CCNNNNNNNGG 3 cut(s) 373, 418, 431
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 444
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 65, 199, 320, 352, 387
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 2 cut(s) 435, 494
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 2 cut(s) 187, 217
BspEI TCCGGA 1 cut(s) 202
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 4 cut(s) 133, 201, 277, 423
BspMI ACCTGC 1 cut(s) 474
BspPI GGATC 3 cut(s) 194, 207, 315
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 5 cut(s) 103, 237, 324, 417, 470
BssMI GATC 5 cut(s) 65, 199, 320, 352, 387
BssT1I CCWWGG 3 cut(s) 237, 324, 470
Bst2UI CCWGG 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 195, 225
BstDSI CCRYGG 2 cut(s) 324, 417
BstF5I GGATG 2 cut(s) 382, 444
BstKTI GATC 5 cut(s) 68, 202, 323, 355, 390
BstMBI GATC 5 cut(s) 65, 199, 320, 352, 387
BstMWI GCNNNNNNNGC 2 cut(s) 475, 487
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 2 cut(s) 214, 453
BstSCI CCNGG 2 cut(s) 127, 372
BstV1I GCAGC 2 cut(s) 490, 502
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 2 cut(s) 324, 417
BtsCI GGATG 2 cut(s) 382, 444
BtsIMutI CAGTG 1 cut(s) 266
BveI ACCTGC 1 cut(s) 474
Cac8I GCNNGC 1 cut(s) 433
CciI TCATGA 1 cut(s) 384
Cfr10I RCCGGY 1 cut(s) 431
Csp6I GTAC 1 cut(s) 113
CviAII CATG 8 cut(s) 13, 153, 211, 271, 325, 368, 385, 450
CviJI RGCY 9 cut(s) 108, 127, 132, 157, 278, 292, 381, 443, 493
CviKI_1 RGCY 9 cut(s) 108, 127, 132, 157, 278, 292, 381, 443, 493
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 2 cut(s) 195, 225
DpnI GATC 5 cut(s) 67, 201, 322, 354, 389
DpnII GATC 5 cut(s) 65, 199, 320, 352, 387
Eco130I CCWWGG 3 cut(s) 237, 324, 470
Eco147I AGGCCT 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 3 cut(s) 237, 324, 470
ErhI CCWWGG 3 cut(s) 237, 324, 470
FaeI CATG 8 cut(s) 16, 156, 214, 274, 328, 371, 388, 453
FatI CATG 8 cut(s) 12, 152, 210, 270, 324, 367, 384, 449
FauI CCCGC 1 cut(s) 428
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 479, 491, 494
FokI GGATG 2 cut(s) 389, 451
Fsp4HI GCNGC 3 cut(s) 479, 491, 494
FspBI CTAG 1 cut(s) 471
GluI GCNGC 3 cut(s) 479, 491, 494
HaeIII GGCC 1 cut(s) 127
HapII CCGG 3 cut(s) 203, 374, 432
Hin1II CATG 8 cut(s) 16, 156, 214, 274, 328, 371, 388, 453
HinfI GANTC 1 cut(s) 77
HpaII CCGG 3 cut(s) 203, 374, 432
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 33, 196
Hpy188III TCNNGA 2 cut(s) 203, 385
Hpy8I GTNNAC 1 cut(s) 463
HpyAV CCTTC 2 cut(s) 289, 329
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 190, 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 487
HpyF3I CTNAG 2 cut(s) 195, 225
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 8 cut(s) 16, 156, 214, 274, 328, 371, 388, 453
Kpn2I TCCGGA 1 cut(s) 202
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 5 cut(s) 65, 199, 320, 352, 387
LmnI GCTCC 2 cut(s) 137, 275
LpnPI CCDG 7 cut(s) 107, 114, 141, 216, 387, 445, 479
Lsp1109I GCAGC 2 cut(s) 490, 502
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 1 cut(s) 398
MalI GATC 5 cut(s) 67, 201, 322, 354, 389
MboI GATC 5 cut(s) 65, 199, 320, 352, 387
MboII GAAGA 3 cut(s) 298, 344, 355
MflI RGATCY 1 cut(s) 199
MluCI AATT 3 cut(s) 85, 117, 171
MnlI CCTC 6 cut(s) 33, 67, 113, 117, 190, 422
MreI CGCCGGCG 1 cut(s) 431
MroI TCCGGA 1 cut(s) 202
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 1 cut(s) 231
MspI CCGG 3 cut(s) 203, 374, 432
MspR9I CCNGG 2 cut(s) 129, 374
Mva1269I GAATGC 1 cut(s) 342
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 475, 487
NaeI GCCGGC 1 cut(s) 433
NciI CCSGG 1 cut(s) 374
NcoI CCATGG 1 cut(s) 324
NdeII GATC 5 cut(s) 65, 199, 320, 352, 387
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 8 cut(s) 16, 156, 214, 274, 328, 371, 388, 453
NlaIV GGNNCC 4 cut(s) 133, 201, 277, 423
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 398
NspI RCATGY 2 cut(s) 214, 453
PagI TCATGA 1 cut(s) 384
PceI AGGCCT 1 cut(s) 127
PctI GAATGC 1 cut(s) 342
PdiI GCCGGC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 480, 492, 495
PshBI ATTAAT 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 4 cut(s) 133, 201, 277, 423
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 3 cut(s) 479, 491, 494
Sau3AI GATC 5 cut(s) 65, 199, 320, 352, 387
ScrFI CCNGG 2 cut(s) 129, 374
SetI ASST 6 cut(s) 294, 340, 351, 414, 468, 476
SgrAI CRCCGGYG 1 cut(s) 431
Sse9I AATT 3 cut(s) 85, 117, 171
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 2 cut(s) 435, 494
SspMI CTAG 1 cut(s) 471
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 2 cut(s) 127, 372
StyI CCWWGG 3 cut(s) 237, 324, 470
TaiI ACGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 390
TasI AATT 3 cut(s) 85, 117, 171
TauI GCSGC 1 cut(s) 496
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 266
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 2 cut(s) 478, 490
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 2 cut(s) 17, 373
TspRI CASTG 1 cut(s) 266
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 1 cut(s) 85
XceI RCATGY 2 cut(s) 214, 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XmaJI CCTAGG 1 cut(s) 470
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.