Rroxscaffold_2G00108340
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012767
Physical Location & Seq
Reverse (-)
32326413 .. 32328046
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108340.1

Sequence Viewer

Length: 513 bp
ATGAAGGAAATTGTGGGAAACCCTCTTCATCTAAAATCTCTGAACCACATCTCACTTATCTGCAGATCAGTTGAAGAATCCATTGATTTTTACCAAAATATTCTTGGGTTTATCCCAATTAGAAGGCCTGGATCATTTGATTTTGATGGGGCATGGTTATTCGGGTATGGAATCGGAATCCATCTTTTGCAATCTGAGGAGCCGGAAAAGATGCCCAAGAAGACTGAGATTAATCCCAAAGATAATCACATTTCTTTTCAGTGTGAGAGCATGGGAGCAGTGGAGAAGAAGCTGAAGGAGATGGAGCTGAACTATAAACGGTCGATGGTGGAGGAAGGCGGTATCCACGTCGATCAGTTGTTCTTCCATGACCCGGATGGCTTCATGATCGAAATATGCGACTGTGACAACCTCCCGGTGATTCCGATAGCCGGCGAGGTGGCTCGGGCATGTTCTCTTGCAAACCTGCCGATGTTGCAGCAGCAACAGCAGATACGAGTCACCCAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

170

Amino Acids

19.36

Weight (kDa)

5.2

Isoelectric Point (pI)

68.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 14 - 132 3.6e-13 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15380 AT1G15380 AT1G80160 AT1G80160
fragaria_vesca FvH4_6g30160
malus_domestica MD17G1207700.v1.1
prunus_persica Prupe.3G082100_v2.0.a1
pyrus_communis pycom17g21020
rosa_chinensis RchiOBHm_Chr3g0458691
rosa_laevigata RLG00000019525
rosa_multiflora Rmu_sc0001998.1_g000022
rosa_roxburghii Rroxscaffold_2G00108340
rosa_rugosa Rorug02G0329400
rosa_samantha Rh2AG380900 Rh2BG374500 Rh2BG387700 Rh2DG404000
rosa_wichuraiana Rw2G031150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 474
AciI CCGC 1 cut(s) 339
AclWI GGATC 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 373, 431
AgsI TTSAA 1 cut(s) 74
AjiI CACGTC 1 cut(s) 349
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 2 cut(s) 292, 307
AluI AGCT 2 cut(s) 292, 307
AlwI GGATC 1 cut(s) 139
Ama87I CYCGRG 1 cut(s) 444
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 478, 481
AseI ATTAAT 1 cut(s) 231
AsuC2I CCSGG 2 cut(s) 374, 416
AsuHPI GGTGA 2 cut(s) 430, 493
AvaI CYCGRG 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 490, 493
BccI CCATC 5 cut(s) 140, 189, 295, 319, 371
BciT130I CCWGG 1 cut(s) 129
BciVI GTATCC 1 cut(s) 353
BcnI CCSGG 2 cut(s) 374, 416
BfmI CTRYAG 1 cut(s) 61
BfuAI ACCTGC 1 cut(s) 474
BfuI GTATCC 1 cut(s) 353
BisI GCNGC 2 cut(s) 479, 482
BlsI GCNGC 2 cut(s) 480, 483
Bme1390I CCNGG 3 cut(s) 129, 374, 416
BmeT110I CYCGRG 1 cut(s) 444
BmgBI CACGTC 1 cut(s) 349
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 3 cut(s) 129, 374, 416
BmsI GCATC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 374, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 431
Bse118I RCCGGY 1 cut(s) 431
BseBI CCWGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 2 cut(s) 373, 431
BseMII CTCAG 2 cut(s) 186, 216
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 490, 493
Bsh1285I CGRYCG 1 cut(s) 323
BshFI GGCC 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 323
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 4 cut(s) 203, 374, 416, 432
BslI CCNNNNNNNGG 2 cut(s) 373, 431
BsnI GGCC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 444
Bsp143I GATC 4 cut(s) 65, 131, 352, 387
BspACI CCGC 1 cut(s) 339
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 2 cut(s) 187, 217
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 65
BspMI ACCTGC 1 cut(s) 474
BspPI GGATC 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssMI GATC 4 cut(s) 65, 131, 352, 387
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 321, 404, 510
Bst6I CTCTTC 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 195, 225
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 4 cut(s) 68, 134, 355, 390
BstMBI GATC 4 cut(s) 65, 131, 352, 387
BstMCI CGRYCG 1 cut(s) 323
BstMWI GCNNNNNNNGC 2 cut(s) 475, 487
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 3 cut(s) 127, 372, 414
BstSFI CTRYAG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 490, 493
BstV2I GAAGAC 1 cut(s) 227
BsuI GTATCC 1 cut(s) 353
BsuRI GGCC 1 cut(s) 127
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 1 cut(s) 382
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 266, 285
BveI ACCTGC 1 cut(s) 474
Cac8I GCNNGC 1 cut(s) 433
CciI TCATGA 1 cut(s) 384
Cfr10I RCCGGY 1 cut(s) 431
CviAII CATG 5 cut(s) 153, 271, 368, 385, 450
CviJI RGCY 7 cut(s) 127, 202, 292, 307, 381, 431, 443
CviKI_1 RGCY 7 cut(s) 127, 202, 292, 307, 381, 431, 443
DdeI CTNAG 2 cut(s) 195, 225
DpnI GATC 4 cut(s) 67, 133, 354, 389
DpnII GATC 4 cut(s) 65, 131, 352, 387
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 127
FaeI CATG 5 cut(s) 156, 274, 371, 388, 453
FaiI YATR 8 cut(s) 154, 168, 272, 315, 369, 386, 397, 451
FatI CATG 5 cut(s) 152, 270, 367, 384, 449
Fnu4HI GCNGC 2 cut(s) 479, 482
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 2 cut(s) 479, 482
GluI GCNGC 2 cut(s) 479, 482
HaeIII GGCC 1 cut(s) 127
HapII CCGG 4 cut(s) 203, 374, 416, 432
Hin1II CATG 5 cut(s) 156, 274, 371, 388, 453
HinfI GANTC 5 cut(s) 77, 171, 177, 421, 498
HpaII CCGG 4 cut(s) 203, 374, 416, 432
HphI GGTGA 2 cut(s) 430, 493
Hpy188I TCNGA 4 cut(s) 42, 176, 196, 426
Hpy188III TCNNGA 1 cut(s) 385
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 3 cut(s) 117, 289, 329
HpyCH4III ACNGT 3 cut(s) 321, 404, 510
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 63, 190, 461, 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 487
HpyF3I CTNAG 2 cut(s) 195, 225
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 5 cut(s) 156, 274, 371, 388, 453
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Kzo9I GATC 4 cut(s) 65, 131, 352, 387
LmnI GCTCC 3 cut(s) 199, 275, 304
LpnPI CCDG 7 cut(s) 114, 141, 216, 387, 429, 445, 479
Lsp1109I GCAGC 2 cut(s) 490, 493
LweI GCATC 1 cut(s) 201
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 404, 499
MalI GATC 4 cut(s) 67, 133, 354, 389
MboI GATC 4 cut(s) 65, 131, 352, 387
MboII GAAGA 5 cut(s) 17, 86, 232, 298, 355
MluCI AATT 2 cut(s) 9, 117
MlyI GAGTC 1 cut(s) 507
MnlI CCTC 5 cut(s) 33, 190, 325, 422, 430
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 1 cut(s) 231
MspI CCGG 4 cut(s) 203, 374, 416, 432
MspR9I CCNGG 3 cut(s) 129, 374, 416
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 475, 487
NaeI GCCGGC 1 cut(s) 433
NciI CCSGG 2 cut(s) 374, 416
NdeII GATC 4 cut(s) 65, 131, 352, 387
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 5 cut(s) 156, 274, 371, 388, 453
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 404, 499
NspI RCATGY 1 cut(s) 453
PagI TCATGA 1 cut(s) 384
PceI AGGCCT 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PdiI GCCGGC 1 cut(s) 433
PfeI GAWTC 4 cut(s) 77, 171, 177, 421
PkrI GCNGC 2 cut(s) 480, 483
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PshBI ATTAAT 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 201
PstI CTGCAG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 479, 482
Sau3AI GATC 4 cut(s) 65, 131, 352, 387
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 3 cut(s) 129, 374, 416
SetI ASST 6 cut(s) 294, 309, 351, 414, 441, 468
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 61
Sse9I AATT 2 cut(s) 9, 117
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 1 cut(s) 339
SspI AATATT 1 cut(s) 100
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 3 cut(s) 127, 372, 414
TaaI ACNGT 3 cut(s) 321, 404, 510
TaiI ACGT 1 cut(s) 351
TaqI TCGA 3 cut(s) 323, 351, 390
TasI AATT 2 cut(s) 9, 117
TfiI GAWTC 4 cut(s) 77, 171, 177, 421
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 266, 285
TseFI GTSAC 2 cut(s) 404, 499
TseI GCWGC 2 cut(s) 478, 481
Tsp45I GTSAC 2 cut(s) 404, 499
TspDTI ATGAA 3 cut(s) 17, 17, 373
TspRI CASTG 2 cut(s) 266, 285
VspI ATTAAT 1 cut(s) 231
XceI RCATGY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 2 cut(s) 101, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.