MD17G1213300.v1.1

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012547
Physical Location & Seq
Forward (+)
26038854 .. 26041640
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1213300.v1.1.491

Sequence Viewer

Length: 900 bp
ATGAAGGGGATGAAGGGGATGATGCCATCTCAATCTTCCGTGAACTACGCTAATGAAGACGGCTACGACGAACTAGAAGAAGAAAATGAAGGATTGTTGAGGAGAGGCATAGTAGAAGGCTGCCGTGGAATTGATGGTATAGCAGACAAGTTTCAGTGCATGAGCATTCACAAGCGAGACGCTGCCAAACGAGGTGAAGAGATCTCTTTTCAGAAGAAATGTCTGTGGAAGCAAGAGCAGAAGGGCAGAGTTGCAAGGCTAGAGAAACAGCTCAGGATAAGGTGGGAACTTGAGGAGCTCATTGAAGAACAACTGAACAGGTTCCATGCCCACTACAACTTGGATATGGTCCCAACCCGGCTTAAGGATGTTGCTCAACTGCTCATGCCCAATTGGACTCCGCCTGATGAGCTGATCTCCACAGCTTGGCTTGGTGATTGGCGTCCCTCTGCAATTCTGGAGCTTGTGCGTAGCTTGCCCATCTGTTCCTGCTCATCATCATCATCCTCTTCGAACTCTAGTCGCACTGAACAGCTCTTATCGCAGCTCATTTATGAGATTCGGATTGAAGAGGCAATAATTGATGAGGAAATGGCTGAAATTCAATCAACATGTATCTTCTACCTTCCATTTGCTCCGGGAAACATCCAATCAGGTAGAGCTGCCTTGGCTTCTGTTCAGTCAGAGTTCAAGAAGATTGAAAGGGTGATCACAAAAGCTCAACAACTTAGGTTCAAGGCACTGGAGTTGGTGTTGAAGAAGGTACTGAATGAAACTGATGCGGCAGAGTTCTTGGTTGCATTCGAGGGAATTCAAGAAGCAATTCACCAGTTTGCAGCAAACCAGAGGTTCCAAAAGGGTCCAGTCACGGTGCCTGTCAAGTCCTTGGGGTCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

300

Amino Acids

33.94

Weight (kDa)

5.63

Isoelectric Point (pI)

68.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 94 - 158 5e-13 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15320 AT1G15320
fragaria_vesca FvH4_6g29541
malus_domestica MD17G1213300.v1.1
prunus_persica Prupe.3G088600_v2.0.a1 Prupe.3G088600_v2.0.a1
pyrus_communis pycom17g21820
rosa_chinensis RchiOBHm_Chr2g0135091
rosa_laevigata RLG00000019441
rosa_multiflora Rmu_co8282347.1_g000001 Rmu_sc0001385.1_g000014 Rmu_sc0003543.1_g000003
rosa_roxburghii Rroxscaffold_2G00109490
rosa_rugosa Rorug02G0322700
rosa_samantha Rh2AG371900 Rh2BG379600 Rh2CG358700 Rh2DG396200
rosa_wichuraiana Rw0G013560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 871
AccB7I CCANNNNNTGG 1 cut(s) 426
AciI CCGC 2 cut(s) 401, 782
AcsI RAATTY 2 cut(s) 600, 810
AcyI GRCGYC 1 cut(s) 442
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 364, 426
AflII CTTAAG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 611
AgsI TTSAA 8 cut(s) 305, 569, 605, 691, 701, 736, 757, 815
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 171
ApeKI GCWGC 5 cut(s) 120, 182, 544, 662, 836
ApoI RAATTY 2 cut(s) 600, 810
Asp700I GAANNNNTTC 2 cut(s) 320, 822
AspS9I GGNCC 2 cut(s) 349, 860
AsuC2I CCSGG 2 cut(s) 358, 639
AsuHPI GGTGA 4 cut(s) 206, 446, 718, 818
AsuII TTCGAA 1 cut(s) 512
AvaII GGWCC 2 cut(s) 349, 860
BanI GGYRCC 1 cut(s) 871
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 5 cut(s) 107, 169, 556, 649, 848
BccI CCATC 3 cut(s) 34, 128, 488
BceAI ACGGC 2 cut(s) 76, 108
BclI TGATCA 1 cut(s) 708
BcnI CCSGG 2 cut(s) 358, 639
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 4 cut(s) 74, 260, 519, 894
BfrI CTTAAG 1 cut(s) 362
BglII AGATCT 1 cut(s) 201
BisI GCNGC 6 cut(s) 121, 183, 545, 663, 783, 837
BlsI GCNGC 6 cut(s) 122, 184, 546, 664, 784, 838
Bme1390I CCNGG 2 cut(s) 358, 639
Bme18I GGWCC 2 cut(s) 349, 860
BmgT120I GGNCC 2 cut(s) 349, 860
BmiI GGNNCC 5 cut(s) 323, 351, 851, 861, 873
BmrFI CCNGG 2 cut(s) 358, 639
BmsI GCATC 2 cut(s) 12, 769
BpiI GAAGAC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 2 cut(s) 479, 764
Bpu10I CCTNAGC 1 cut(s) 272
Bpu14I TTCGAA 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 2 cut(s) 358, 639
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 3 cut(s) 124, 666, 885
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 364, 426
Bse1I ACTGG 3 cut(s) 747, 829, 863
BseDI CCNNGG 3 cut(s) 124, 666, 885
BseGI GGATG 5 cut(s) 15, 24, 373, 503, 645
BseLI CCNNNNNNNGG 2 cut(s) 364, 426
BseMII CTCAG 1 cut(s) 286
BseNI ACTGG 3 cut(s) 747, 829, 863
BseRI GAGGAG 2 cut(s) 115, 308
BseXI GCAGC 5 cut(s) 107, 169, 556, 649, 848
BshNI GGYRCC 1 cut(s) 871
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 2 cut(s) 358, 638
BslFI GGGAC 2 cut(s) 335, 429
BslI CCNNNNNNNGG 2 cut(s) 364, 426
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 2 cut(s) 335, 429
BsmI GAATGC 2 cut(s) 165, 800
Bsp119I TTCGAA 1 cut(s) 512
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 3 cut(s) 201, 414, 708
BspACI CCGC 2 cut(s) 401, 782
BspCNI CTCAG 1 cut(s) 285
BspLI GGNNCC 5 cut(s) 323, 351, 851, 861, 873
BspT104I TTCGAA 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 871
BspTI CTTAAG 1 cut(s) 362
BsrI ACTGG 3 cut(s) 747, 829, 863
BssECI CCNNGG 3 cut(s) 124, 666, 885
BssMI GATC 3 cut(s) 201, 414, 708
BssNI GRCGYC 1 cut(s) 442
BssT1I CCWWGG 2 cut(s) 666, 885
Bst4CI ACNGT 1 cut(s) 871
Bst6I CTCTTC 3 cut(s) 192, 514, 564
BstACI GRCGYC 1 cut(s) 442
BstAFI CTTAAG 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 2 cut(s) 272, 728
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 5 cut(s) 15, 24, 373, 503, 645
BstKTI GATC 3 cut(s) 204, 417, 711
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 3 cut(s) 201, 414, 708
BstMWI GCNNNNNNNGC 4 cut(s) 409, 475, 541, 668
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 2 cut(s) 356, 637
BstV1I GCAGC 5 cut(s) 107, 169, 556, 649, 848
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 5 cut(s) 15, 24, 373, 503, 645
BtsIMutI CAGTG 3 cut(s) 161, 525, 740
Cac8I GCNNGC 1 cut(s) 476
Cfr13I GGNCC 2 cut(s) 349, 860
CseI GACGC 2 cut(s) 188, 431
Csp6I GTAC 1 cut(s) 764
CviAII CATG 4 cut(s) 160, 326, 385, 612
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 2 cut(s) 272, 728
DpnI GATC 3 cut(s) 203, 416, 710
DpnII GATC 3 cut(s) 201, 414, 708
Eam1104I CTCTTC 3 cut(s) 192, 514, 564
EarI CTCTTC 3 cut(s) 192, 514, 564
EciI GGCGGA 1 cut(s) 390
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 2 cut(s) 666, 885
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 349, 860
Eco53kI GAGCTC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 810
EcoT14I CCWWGG 2 cut(s) 666, 885
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 2 cut(s) 666, 885
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 4 cut(s) 163, 329, 388, 615
FaiI YATR 8 cut(s) 110, 140, 161, 327, 347, 386, 555, 613
FaqI GGGAC 2 cut(s) 335, 429
FatI CATG 4 cut(s) 159, 325, 384, 611
FbaI TGATCA 1 cut(s) 708
Fnu4HI GCNGC 6 cut(s) 121, 183, 545, 663, 783, 837
FokI GGATG 5 cut(s) 22, 31, 380, 490, 632
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 6 cut(s) 121, 183, 545, 663, 783, 837
FspBI CTAG 4 cut(s) 74, 260, 519, 894
GluI GCNGC 6 cut(s) 121, 183, 545, 663, 783, 837
GsuI CTGGAG 2 cut(s) 479, 764
HapII CCGG 2 cut(s) 358, 638
HgaI GACGC 2 cut(s) 188, 431
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 4 cut(s) 163, 329, 388, 615
HinfI GANTC 2 cut(s) 397, 559
HpaII CCGG 2 cut(s) 358, 638
HphI GGTGA 4 cut(s) 206, 446, 718, 818
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 213, 564, 685
Hpy188III TCNNGA 4 cut(s) 274, 458, 691, 815
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 6 cut(s) 7, 83, 110, 235, 635, 754
HpyCH4III ACNGT 1 cut(s) 871
HpyCH4V TGCA 5 cut(s) 159, 254, 452, 800, 836
HpyF10VI GCNNNNNNNGC 4 cut(s) 409, 475, 541, 668
HpyF3I CTNAG 2 cut(s) 272, 728
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 4 cut(s) 163, 329, 388, 615
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 3 cut(s) 201, 414, 708
LmnI GCTCC 3 cut(s) 295, 460, 640
Lsp1109I GCAGC 5 cut(s) 107, 169, 556, 649, 848
LweI GCATC 2 cut(s) 12, 769
MaeI CTAG 4 cut(s) 74, 260, 519, 894
MaeIII GTNAC 1 cut(s) 865
MalI GATC 3 cut(s) 203, 416, 710
MboI GATC 3 cut(s) 201, 414, 708
MfeI CAATTG 1 cut(s) 391
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 7 cut(s) 129, 391, 453, 579, 600, 810, 822
MlyI GAGTC 1 cut(s) 391
MroXI GAANNNNTTC 2 cut(s) 320, 822
MseI TTAA 1 cut(s) 363
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 2 cut(s) 358, 638
MspR9I CCNGG 2 cut(s) 358, 639
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 2 cut(s) 165, 800
MwoI GCNNNNNNNGC 4 cut(s) 409, 475, 541, 668
NciI CCSGG 2 cut(s) 358, 639
NdeII GATC 3 cut(s) 201, 414, 708
NlaIII CATG 4 cut(s) 163, 329, 388, 615
NlaIV GGNNCC 5 cut(s) 323, 351, 851, 861, 873
NmuCI GTSAC 1 cut(s) 865
NspI RCATGY 1 cut(s) 615
NspV TTCGAA 1 cut(s) 512
PciI ACATGT 1 cut(s) 611
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 2 cut(s) 165, 800
PdmI GAANNNNTTC 2 cut(s) 320, 822
PfeI GAWTC 1 cut(s) 559
PflMI CCANNNNNTGG 1 cut(s) 426
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 6 cut(s) 122, 184, 546, 664, 784, 838
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PscI ACATGT 1 cut(s) 611
Psp124BI GAGCTC 1 cut(s) 300
PspN4I GGNNCC 5 cut(s) 323, 351, 851, 861, 873
PspPI GGNCC 2 cut(s) 349, 860
PsuI RGATCY 1 cut(s) 201
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 6 cut(s) 121, 183, 545, 663, 783, 837
Sau3AI GATC 3 cut(s) 201, 414, 708
Sau96I GGNCC 2 cut(s) 349, 860
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 2 cut(s) 358, 639
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 2 cut(s) 12, 769
SfuI TTCGAA 1 cut(s) 512
SinI GGWCC 2 cut(s) 349, 860
SmlI CTYRAG 2 cut(s) 290, 362
SmoI CTYRAG 2 cut(s) 290, 362
Sse9I AATT 7 cut(s) 129, 391, 453, 579, 600, 810, 822
SsiI CCGC 2 cut(s) 401, 782
SspMI CTAG 4 cut(s) 74, 260, 519, 894
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 2 cut(s) 356, 637
StyI CCWWGG 2 cut(s) 666, 885
TaaI ACNGT 1 cut(s) 871
TaqI TCGA 2 cut(s) 512, 804
TasI AATT 7 cut(s) 129, 391, 453, 579, 600, 810, 822
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 1 cut(s) 559
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 3 cut(s) 161, 532, 747
TseFI GTSAC 1 cut(s) 865
TseI GCWGC 5 cut(s) 120, 182, 544, 662, 836
Tsp45I GTSAC 1 cut(s) 865
TspDTI ATGAA 5 cut(s) 17, 26, 69, 102, 786
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 3 cut(s) 161, 532, 747
Van91I CCANNNNNTGG 1 cut(s) 426
Vha464I CTTAAG 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 349, 860
XapI RAATTY 2 cut(s) 600, 810
XceI RCATGY 1 cut(s) 615
XmnI GAANNNNTTC 2 cut(s) 320, 822
XspI CTAG 4 cut(s) 74, 260, 519, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.