Rorug02G0322700

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001801
Physical Location & Seq
Reverse (-)
39507629 .. 39513843
6215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0322700.1

Sequence Viewer

Length: 1041 bp
ATGGAGCTCAATATAACACGCCCTGATGATTGGCATCTTCACCTTCGTGACGGTGAACTTCTTGAAGCTGTCGTCCCCCACAGTGCAACTCATTTTGGACGGGGAATAGTGATGCCAAATCTAAAGCCCCCTGTCACCACCACTGCTGCAGCTGTGGCTTACCGAGAATCTATCTTAAAAGCACTGCCTTCTGGTAGTGGCTTCACTCCTCTTATGACACTTTATTTGACAGATACCACTAGTCCTAAAGAGATCAAGCTTGCAAGAAAAAGTGGGGTCGTATTTGCTGTAAAGCTTTACCCTGCTGGTGCCACAACAAATTCCCAAGATGGAGTCACAGATCTTTTTGGAAAATGCCTACCTGTCCTTGAGGAGATGGTTGAACAAAATATGCCTCTGCTGGTTCATGGGGAGGTTACAGATCCTGATGTTGATGTATTTGATCGTGAAAAGGTATTCATCGACTCTGTTTTACAACCTTTAATCAAAAGGCTTCCACAGCTGAAGGTCGTCATGGAGCATATAACTACTATGGATGCTGTTAAGTTTGTTCAGTCTTGCGAAGAAGGTTCTGTAGCAGCAACAGTTACTCCACAGCATCTTCTTCTGAATAGGAATGCTCTATTTCAAGGAGGATTGCAGCCTCACAACTACTGTCTGCCTGTACTCAAAAGGGAGATCCACAGACAGGCTCTTGTATCAGCTGTTACTAGTGGAAGTAAAAGATTTTTTCTTGGAACTGATAGTGCTCCACATGAAAAGCGAAGAAAAGAATGTCCTTGTGGTTGTGCTGGTATATACAGTGCCCCTGTTGCTCTATCATTGTATGCAAAGGTCTTTGAAGAGGCAGGTGCTCTTGACAAGCTAGAGGCATTTACAAGCTTTAATGGGCCAGACTTTTATGGCCTTCCTCGGAATACGTCAAAAACAAAGCTGAGCAGAACTTCATGGAAGGTACCAGAGTGCTTCTCATTTCCATTTGGAGACATCATACCAATGTTTGCAGGAGAAATGCTTGAGTGGCAGTCGTCCTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

346

Amino Acids

38.18

Weight (kDa)

6.37

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 10 - 306 9.7e-23 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 839
Acc36I ACCTGC 1 cut(s) 839
Acc65I GGTACC 1 cut(s) 955
AccB1I GGYRCC 2 cut(s) 308, 955
AclWI GGATC 2 cut(s) 416, 673
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 2 cut(s) 666, 957
AfiI CCNNNNNNNGG 1 cut(s) 688
AgsI TTSAA 5 cut(s) 65, 383, 629, 842, 1036
AhlI ACTAGT 2 cut(s) 239, 710
AleI CACNNNNGTG 1 cut(s) 45
Alw21I GWGCWC 3 cut(s) 9, 751, 856
Alw26I GTCTC 1 cut(s) 978
AlwI GGATC 2 cut(s) 416, 673
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 2 cut(s) 890, 904
ApeKI GCWGC 4 cut(s) 146, 149, 578, 640
ApoI RAATTY 1 cut(s) 319
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
Asp718I GGTACC 1 cut(s) 955
AspS9I GGNCC 1 cut(s) 890
AsuHPI GGTGA 3 cut(s) 32, 65, 127
BaeGI GKGCMC 1 cut(s) 808
BaeI ACNNNNGTAYC 2 cut(s) 947, 980
BanI GGYRCC 2 cut(s) 308, 955
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 3 cut(s) 9, 751, 856
BbvI GCAGC 4 cut(s) 133, 161, 590, 652
BccI CCATC 2 cut(s) 323, 370
BcoDI GTCTC 1 cut(s) 978
BcuI ACTAGT 2 cut(s) 239, 710
BfaI CTAG 3 cut(s) 240, 711, 866
BfmI CTRYAG 2 cut(s) 147, 573
BfuAI ACCTGC 1 cut(s) 839
BglII AGATCT 1 cut(s) 340
BisI GCNGC 4 cut(s) 147, 150, 579, 641
BlpI GCTNAGC 1 cut(s) 935
BlsI GCNGC 4 cut(s) 148, 151, 580, 642
BmgT120I GGNCC 1 cut(s) 890
BmiI GGNNCC 2 cut(s) 310, 957
BmsI GCATC 4 cut(s) 43, 102, 526, 607
BplI GAGNNNNNCTC 2 cut(s) 953, 985
Bpu1102I GCTNAGC 1 cut(s) 935
BpuEI CTTGAG 2 cut(s) 389, 1037
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 911
BsaXI ACNNNNNCTCC 2 cut(s) 574, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse8I GATNNNNATC 1 cut(s) 33
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 1 cut(s) 541
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 1 cut(s) 926
BseRI GAGGAG 2 cut(s) 198, 386
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 4 cut(s) 133, 161, 590, 652
BshFI GGCC 2 cut(s) 892, 906
BshNI GGYRCC 2 cut(s) 308, 955
BsiHKAI GWGCWC 3 cut(s) 9, 751, 856
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 1 cut(s) 978
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 2 cut(s) 892, 906
Bsp1286I GDGCHC 4 cut(s) 9, 751, 808, 856
Bsp143I GATC 5 cut(s) 252, 340, 421, 442, 678
Bsp1720I GCTNAGC 1 cut(s) 935
BspANI GGCC 2 cut(s) 892, 906
BspCNI CTCAG 1 cut(s) 927
BspLI GGNNCC 2 cut(s) 310, 957
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 2 cut(s) 416, 673
BspT107I GGYRCC 2 cut(s) 308, 955
BssECI CCNNGG 1 cut(s) 911
BssMI GATC 5 cut(s) 252, 340, 421, 442, 678
Bst4CI ACNGT 5 cut(s) 53, 83, 586, 656, 803
Bst6I CTCTTC 1 cut(s) 837
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 935
BstF5I GGATG 1 cut(s) 541
BstKTI GATC 5 cut(s) 255, 343, 424, 445, 681
BstMAI GTCTC 1 cut(s) 978
BstMBI GATC 5 cut(s) 252, 340, 421, 442, 678
BstMWI GCNNNNNNNGC 4 cut(s) 155, 499, 812, 1021
BstSFI CTRYAG 2 cut(s) 147, 573
BstSLI GKGCMC 1 cut(s) 808
BstV1I GCAGC 4 cut(s) 133, 161, 590, 652
BstX2I RGATCY 3 cut(s) 340, 421, 678
BstYI RGATCY 3 cut(s) 340, 421, 678
BsuRI GGCC 2 cut(s) 892, 906
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 2 cut(s) 141, 182
BtsIMutI CAGTG 4 cut(s) 88, 141, 182, 808
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 1 cut(s) 261
CaiI CAGNNNCTG 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 890
Csp6I GTAC 2 cut(s) 665, 956
CviAII CATG 4 cut(s) 407, 514, 755, 948
CviQI GTAC 2 cut(s) 665, 956
DdeI CTNAG 1 cut(s) 935
DpnI GATC 5 cut(s) 254, 342, 423, 444, 680
DpnII GATC 5 cut(s) 252, 340, 421, 442, 678
Eam1104I CTCTTC 1 cut(s) 837
EarI CTCTTC 1 cut(s) 837
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 7
EcoT38I GRGCYC 1 cut(s) 9
FaeI CATG 4 cut(s) 410, 517, 758, 951
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 406, 513, 754, 947
Fnu4HI GCNGC 4 cut(s) 147, 150, 579, 641
FokI GGATG 1 cut(s) 548
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 4 cut(s) 147, 150, 579, 641
FspBI CTAG 3 cut(s) 240, 711, 866
GluI GCNGC 4 cut(s) 147, 150, 579, 641
HaeIII GGCC 2 cut(s) 892, 906
Hin1II CATG 4 cut(s) 410, 517, 758, 951
HindIII AAGCTT 3 cut(s) 257, 293, 880
HinfI GANTC 3 cut(s) 167, 333, 464
HphI GGTGA 3 cut(s) 32, 65, 127
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 2 cut(s) 609, 915
Hpy188III TCNNGA 5 cut(s) 47, 62, 425, 446, 857
Hpy8I GTNNAC 1 cut(s) 56
HpyAV CCTTC 6 cut(s) 53, 198, 499, 560, 917, 946
HpyCH4III ACNGT 5 cut(s) 53, 83, 586, 656, 803
HpyCH4IV ACGT 1 cut(s) 920
HpyCH4V TGCA 6 cut(s) 86, 149, 263, 640, 830, 1004
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 499, 812, 1021
HpyF3I CTNAG 1 cut(s) 935
HpySE526I ACGT 1 cut(s) 920
Hsp92II CATG 4 cut(s) 410, 517, 758, 951
KpnI GGTACC 1 cut(s) 959
Kzo9I GATC 5 cut(s) 252, 340, 421, 442, 678
LmnI GCTCC 3 cut(s) 4, 517, 754
Lsp1109I GCAGC 4 cut(s) 133, 161, 590, 652
LweI GCATC 4 cut(s) 43, 102, 526, 607
MaeI CTAG 3 cut(s) 240, 711, 866
MaeII ACGT 1 cut(s) 920
MaeIII GTNAC 6 cut(s) 47, 133, 334, 415, 586, 706
MalI GATC 5 cut(s) 254, 342, 423, 444, 680
MboI GATC 5 cut(s) 252, 340, 421, 442, 678
MboII GAAGA 6 cut(s) 29, 575, 593, 596, 777, 854
MfeI CAATTG 1 cut(s) 1036
MflI RGATCY 3 cut(s) 340, 421, 678
MhlI GDGCHC 4 cut(s) 9, 751, 808, 856
MluCI AATT 2 cut(s) 319, 1036
MlyI GAGTC 2 cut(s) 342, 458
MnlI CCTC 9 cut(s) 219, 364, 405, 406, 626, 654, 838, 862, 921
MseI TTAA 4 cut(s) 176, 482, 543, 885
MslI CAYNNNNRTG 2 cut(s) 45, 995
MspA1I CMGCKG 3 cut(s) 152, 502, 704
MunI CAATTG 1 cut(s) 1036
Mva1269I GAATGC 1 cut(s) 622
MwoI GCNNNNNNNGC 4 cut(s) 155, 499, 812, 1021
NdeII GATC 5 cut(s) 252, 340, 421, 442, 678
NlaIII CATG 4 cut(s) 410, 517, 758, 951
NlaIV GGNNCC 2 cut(s) 310, 957
NmuCI GTSAC 3 cut(s) 47, 133, 334
OliI CACNNNNGTG 1 cut(s) 45
PaqCI CACCTGC 1 cut(s) 839
PctI GAATGC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 4 cut(s) 148, 151, 580, 642
PleI GAGTC 2 cut(s) 341, 458
PpsI GAGTC 2 cut(s) 341, 458
Psp124BI GAGCTC 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 310, 957
PspPI GGNCC 1 cut(s) 890
PstI CTGCAG 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 3 cut(s) 340, 421, 678
PvuII CAGCTG 3 cut(s) 152, 502, 704
RsaI GTAC 2 cut(s) 666, 957
RsaNI GTAC 2 cut(s) 665, 956
RseI CAYNNNNRTG 2 cut(s) 45, 995
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 4 cut(s) 176, 482, 543, 885
SatI GCNGC 4 cut(s) 147, 150, 579, 641
Sau3AI GATC 5 cut(s) 252, 340, 421, 442, 678
Sau96I GGNCC 1 cut(s) 890
SchI GAGTC 2 cut(s) 342, 458
SduI GDGCHC 4 cut(s) 9, 751, 808, 856
SfaNI GCATC 4 cut(s) 43, 102, 526, 607
SfcI CTRYAG 2 cut(s) 147, 573
SmiMI CAYNNNNRTG 2 cut(s) 45, 995
SmlI CTYRAG 2 cut(s) 368, 1016
SmoI CTYRAG 2 cut(s) 368, 1016
SpeI ACTAGT 2 cut(s) 239, 710
Sse9I AATT 2 cut(s) 319, 1036
SspMI CTAG 3 cut(s) 240, 711, 866
SstI GAGCTC 1 cut(s) 9
TaaI ACNGT 5 cut(s) 53, 83, 586, 656, 803
TaiI ACGT 1 cut(s) 923
TaqI TCGA 1 cut(s) 462
TasI AATT 2 cut(s) 319, 1036
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 4 cut(s) 176, 482, 543, 885
Tru9I TTAA 4 cut(s) 176, 482, 543, 885
TscAI CASTG 4 cut(s) 88, 148, 189, 808
TseFI GTSAC 3 cut(s) 47, 133, 334
TseI GCWGC 4 cut(s) 146, 149, 578, 640
Tsp45I GTSAC 3 cut(s) 47, 133, 334
TspDTI ATGAA 4 cut(s) 395, 448, 771, 936
TspRI CASTG 4 cut(s) 88, 148, 189, 808
XapI RAATTY 1 cut(s) 319
XspI CTAG 3 cut(s) 240, 711, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.