MD17G1221700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016224
Physical Location & Seq
Forward (+)
27024283 .. 27024723
441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1221700.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGGCGACAATGACCCGTGGTTAGCCCCAGACAAGCTCCAACATGTTCTCTTCTGCTTCTCTCTCACGCTCATCTTCTCCACTCTAGCCACCCGAACCCGGTACCCGTTTCTCCGTCGTCACTCTATTTGGTTCGGATCCATCTTGTCTCTCTTAGCCGGCGCAGTCAAGGAGTTCGCTGACGAGCTCGGGTTCTTCAGGTCCGCCGGAGCCTCCGCTAAAGACGCCGTTGCCGATTTCGTTGGCGTCCTAATCGGCTCGCTGGTGCTTTATTTTGTCAAGTATGTGACTCGACCCGAGAATGAGACGGGTCGGCTCAAAGAGGTTTCGATGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

12.51

Weight (kDa)

7.9

Isoelectric Point (pI)

25.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15900
fragaria_vesca FvH4_6g28800
malus_domestica MD16G1196200.v1.1 MD17G1221700.v1.1
prunus_persica Prupe.3G096700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0133221
rosa_laevigata RLG00000019322
rosa_multiflora Rmu_sc0023254.1_g000005
rosa_roxburghii Rroxscaffold_2G00111290
rosa_rugosa Rorug02G0310800
rosa_samantha Rh2BG369000 Rh2CG346600 Rh2DG386000
rosa_wichuraiana Rw2G029530 Rw2G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 102
AciI CCGC 2 cut(s) 204, 216
AclWI GGATC 2 cut(s) 132, 145
AcuI CTGAAG 1 cut(s) 181
AcyI GRCGYC 2 cut(s) 225, 246
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 99
AflIII ACRYGT 1 cut(s) 43
AluBI AGCT 2 cut(s) 37, 187
AluI AGCT 2 cut(s) 37, 187
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 153, 299
AlwI GGATC 2 cut(s) 132, 145
Ama87I CYCGRG 2 cut(s) 188, 296
Asp718I GGTACC 1 cut(s) 102
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 201
AsuC2I CCSGG 1 cut(s) 100
AvaI CYCGRG 2 cut(s) 188, 296
AvaII GGWCC 1 cut(s) 201
BamHI GGATCC 1 cut(s) 137
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 189
BccI CCATC 2 cut(s) 149, 325
BceAI ACGGC 1 cut(s) 212
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 153, 299
BfaI CTAG 1 cut(s) 86
Bme1390I CCNGG 1 cut(s) 100
Bme18I GGWCC 1 cut(s) 201
BmeT110I CYCGRG 2 cut(s) 188, 296
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 3 cut(s) 104, 139, 211
BmrFI CCNGG 1 cut(s) 100
BpuMI CCSGG 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 225, 246
BsaJI CCNNGG 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 17
BseLI CCNNNNNNNGG 1 cut(s) 99
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 2 cut(s) 188, 296
BsiSI CCGG 3 cut(s) 100, 159, 207
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 153, 299
BsmBI CGTCTC 1 cut(s) 299
BsoBI CYCGRG 2 cut(s) 188, 296
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 137
BspACI CCGC 2 cut(s) 204, 216
BspLI GGNNCC 3 cut(s) 104, 139, 211
BspPI GGATC 2 cut(s) 132, 145
BspT107I GGYRCC 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 1 cut(s) 137
BssNI GRCGYC 2 cut(s) 225, 246
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 2 cut(s) 225, 246
BstC8I GCNNGC 2 cut(s) 160, 260
BstDEI CTNAG 1 cut(s) 154
BstDSI CCRYGG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 140
BstMAI GTCTC 2 cut(s) 153, 299
BstMBI GATC 1 cut(s) 137
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 160, 260
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 201
CseI GACGC 2 cut(s) 233, 235
Csp6I GTAC 1 cut(s) 103
CviAII CATG 1 cut(s) 44
CviJI RGCY 8 cut(s) 26, 37, 89, 158, 187, 212, 258, 316
CviKI_1 RGCY 8 cut(s) 26, 37, 89, 158, 187, 212, 258, 316
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 1 cut(s) 154
DpnI GATC 1 cut(s) 139
DpnII GATC 1 cut(s) 137
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 181
Eco88I CYCGRG 2 cut(s) 188, 296
EcoICRI GAGCTC 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 1 cut(s) 47
FaiI YATR 2 cut(s) 45, 285
FatI CATG 1 cut(s) 43
FriOI GRGCYC 1 cut(s) 189
FspBI CTAG 1 cut(s) 86
GlaI GCGC 1 cut(s) 163
HapII CCGG 3 cut(s) 100, 159, 207
HgaI GACGC 2 cut(s) 233, 235
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 2 cut(s) 225, 246
Hin1II CATG 1 cut(s) 47
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HinfI GANTC 1 cut(s) 289
HpaII CCGG 3 cut(s) 100, 159, 207
Hpy188I TCNGA 2 cut(s) 137, 338
Hpy99I CGWCG 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 1 cut(s) 154
Hsp92I GRCGYC 2 cut(s) 225, 246
Hsp92II CATG 1 cut(s) 47
HspAI GCGC 1 cut(s) 162
KpnI GGTACC 1 cut(s) 106
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Kzo9I GATC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 42, 209
LpnPI CCDG 6 cut(s) 42, 113, 172, 184, 220, 248
MaeI CTAG 1 cut(s) 86
MaeIII GTNAC 2 cut(s) 119, 286
MalI GATC 1 cut(s) 139
MboI GATC 1 cut(s) 137
MboII GAAGA 3 cut(s) 43, 67, 187
MflI RGATCY 1 cut(s) 137
MhlI GDGCHC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 2 cut(s) 223, 316
MroNI GCCGGC 1 cut(s) 158
MspI CCGG 3 cut(s) 100, 159, 207
MspR9I CCNGG 1 cut(s) 100
MwoI GCNNNNNNNGC 1 cut(s) 224
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 1 cut(s) 100
NdeII GATC 1 cut(s) 137
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 1 cut(s) 47
NlaIV GGNNCC 3 cut(s) 104, 139, 211
NmuCI GTSAC 2 cut(s) 119, 286
NspI RCATGY 1 cut(s) 47
PciI ACATGT 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdiI GCCGGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PscI ACATGT 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 189
PspN4I GGNNCC 3 cut(s) 104, 139, 211
PspPI GGNCC 1 cut(s) 201
PsuI RGATCY 1 cut(s) 137
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SacI GAGCTC 1 cut(s) 189
Sau3AI GATC 1 cut(s) 137
Sau96I GGNCC 1 cut(s) 201
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 1 cut(s) 189
SetI ASST 4 cut(s) 39, 189, 203, 327
SinI GGWCC 1 cut(s) 201
SsiI CCGC 2 cut(s) 204, 216
SspMI CTAG 1 cut(s) 86
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 98
TaqI TCGA 2 cut(s) 292, 329
TseFI GTSAC 2 cut(s) 119, 286
Tsp45I GTSAC 2 cut(s) 119, 286
TspGWI ACGGA 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 201
XceI RCATGY 1 cut(s) 47
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.