MD17G1256500.v1.1

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016742
Physical Location & Seq
Forward (+)
31704243 .. 31704683
441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1256500.v1.1.491

Sequence Viewer

Length: 441 bp
ATGCTTTTGATTTGTTCTTTAGCAAGTGTCACCGCCACTACTTCTGATGATCATCAACCTTTGGTGTCTAAGGAAGTGGACGCCGGGATAAAATGTGGAACGTGCCCTTGTGTCAACCCATGTGAACAGAAACAGCTGTCTCCACCGCCACCGCCACCGCCAAAGACTCCAACGACAACTCAAGATTGCAACCCTAATCCAAGCCAATACGTGCCAGTAGCGCCGCCGCCTCCAAGGTTTGTTTACGTGACAGGTCTACCTGGAGATGTACCGCCGCCACCTCCGAGGTTTGTTTACGCGACAGGTCTACCTGGAGATGTGTATCAGTACCAAACCGATGCTTATGCATATTACTCAGCTGCTCCACGTCATCACTACAGCATGGGGTTGCTACTCTTGTTGGTATGTGCAGAAGTAATAATGGCTTTTGGGGGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

147

Amino Acids

15.6

Weight (kDa)

5.39

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29110
malus_domestica MD17G1256500.v1.1
prunus_persica Prupe.3G159100_v2.0.a1
pyrus_communis pycom17g25870
rosa_chinensis RchiOBHm_Chr3g0493521
rosa_laevigata RLG00000022709
rosa_multiflora Rmu_sc0002343.1_g000062
rosa_roxburghii Rroxscaffold_6G00391520
rosa_rugosa Rorug03G0261700
rosa_samantha Rh3AG310600 Rh3BG346800 Rh3CG344600 Rh3DG345900
rosa_wichuraiana Rw3G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 256, 307
AccII CGCG 1 cut(s) 299
AciI CCGC 8 cut(s) 33, 146, 152, 158, 224, 227, 272, 275
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 270, 329
AjiI CACGTC 1 cut(s) 368
AjnI CCWGG 2 cut(s) 259, 310
AluBI AGCT 2 cut(s) 136, 359
AluI AGCT 2 cut(s) 136, 359
Alw26I GTCTC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 359
AspLEI GCGC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 2 cut(s) 261, 312
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 376
BfoI RGCGCY 1 cut(s) 224
BisI GCNGC 4 cut(s) 224, 227, 275, 360
BlsI GCNGC 4 cut(s) 225, 228, 276, 361
Bme1390I CCNGG 3 cut(s) 85, 261, 312
BmgBI CACGTC 1 cut(s) 368
BmrFI CCNGG 3 cut(s) 85, 261, 312
BmsI GCATC 1 cut(s) 328
BpmI CTGGAG 2 cut(s) 282, 333
BpuEI CTTGAG 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 2 cut(s) 211, 247
BsaBI GATNNNNATC 2 cut(s) 51, 321
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 233, 284
Bse1I ACTGG 1 cut(s) 215
Bse8I GATNNNNATC 2 cut(s) 51, 321
BseBI CCWGG 2 cut(s) 261, 312
BseDI CCNNGG 2 cut(s) 233, 284
BseJI GATNNNNATC 2 cut(s) 51, 321
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 215
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 8 cut(s) 33, 146, 152, 158, 224, 227, 272, 275
BspCNI CTCAG 1 cut(s) 368
BspFNI CGCG 1 cut(s) 299
BsrI ACTGG 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 233, 284
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 233
Bst2UI CCWGG 2 cut(s) 261, 312
BstACI GRCGYC 1 cut(s) 81
BstBAI YACGTR 2 cut(s) 211, 247
BstDEI CTNAG 2 cut(s) 69, 355
BstFNI CGCG 1 cut(s) 299
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 220
BstNI CCWGG 2 cut(s) 261, 312
BstSCI CCNGG 3 cut(s) 83, 259, 310
BstSFI CTRYAG 1 cut(s) 376
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 1 cut(s) 346
BtrI CACGTC 1 cut(s) 368
CfoI GCGC 1 cut(s) 223
CseI GACGC 1 cut(s) 89
Csp6I GTAC 2 cut(s) 269, 328
CviAII CATG 2 cut(s) 120, 382
CviJI RGCY 4 cut(s) 136, 204, 359, 425
CviKI_1 RGCY 4 cut(s) 136, 204, 359, 425
CviQI GTAC 2 cut(s) 269, 328
DdeI CTNAG 2 cut(s) 69, 355
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 259, 310
EcoT14I CCWWGG 1 cut(s) 233
EcoT22I ATGCAT 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 233
FaeI CATG 2 cut(s) 123, 385
FaiI YATR 5 cut(s) 121, 345, 349, 383, 406
FatI CATG 2 cut(s) 119, 381
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 2 cut(s) 256, 307
Fnu4HI GCNGC 4 cut(s) 224, 227, 275, 360
Fsp4HI GCNGC 4 cut(s) 224, 227, 275, 360
GlaI GCGC 1 cut(s) 222
GluI GCNGC 4 cut(s) 224, 227, 275, 360
GsuI CTGGAG 2 cut(s) 282, 333
HaeII RGCGCY 1 cut(s) 224
HapII CCGG 1 cut(s) 84
HgaI GACGC 1 cut(s) 89
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 2 cut(s) 123, 385
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 166, 435
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 7 cut(s) 79, 115, 125, 244, 257, 295, 308
Hpy188I TCNGA 3 cut(s) 46, 285, 440
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 7 cut(s) 79, 115, 125, 244, 257, 295, 308
HpyCH4IV ACGT 4 cut(s) 101, 210, 246, 367
HpyCH4V TGCA 3 cut(s) 189, 347, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 220
HpyF3I CTNAG 2 cut(s) 69, 355
HpySE526I ACGT 4 cut(s) 101, 210, 246, 367
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 2 cut(s) 123, 385
HspAI GCGC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 8 cut(s) 97, 228, 237, 246, 273, 288, 297, 324
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 1 cut(s) 328
MaeII ACGT 4 cut(s) 101, 210, 246, 367
MaeIII GTNAC 2 cut(s) 28, 247
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 107
MlyI GAGTC 2 cut(s) 160, 429
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 3 cut(s) 240, 279, 291
Mph1103I ATGCAT 1 cut(s) 349
MspA1I CMGCKG 2 cut(s) 136, 359
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 3 cut(s) 85, 261, 312
MvaI CCWGG 2 cut(s) 261, 312
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 85
NdeII GATC 1 cut(s) 49
NlaIII CATG 2 cut(s) 123, 385
NmuCI GTSAC 2 cut(s) 28, 247
NsiI ATGCAT 1 cut(s) 349
PkrI GCNGC 4 cut(s) 225, 228, 276, 361
PleI GAGTC 2 cut(s) 160, 429
PpsI GAGTC 2 cut(s) 160, 429
Ppu21I YACGTR 2 cut(s) 211, 247
Psp6I CCWGG 2 cut(s) 259, 310
PspGI CCWGG 2 cut(s) 259, 310
PvuII CAGCTG 2 cut(s) 136, 359
RsaI GTAC 2 cut(s) 270, 329
RsaNI GTAC 2 cut(s) 269, 328
SatI GCNGC 4 cut(s) 224, 227, 275, 360
Sau3AI GATC 1 cut(s) 49
SchI GAGTC 2 cut(s) 160, 429
ScrFI CCNGG 3 cut(s) 85, 261, 312
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
SsiI CCGC 8 cut(s) 33, 146, 152, 158, 224, 227, 272, 275
StyD4I CCNGG 3 cut(s) 83, 259, 310
StyI CCWWGG 1 cut(s) 233
TaiI ACGT 4 cut(s) 104, 213, 249, 370
TauI GCSGC 3 cut(s) 226, 229, 277
TseFI GTSAC 2 cut(s) 28, 247
TseI GCWGC 1 cut(s) 359
Tsp45I GTSAC 2 cut(s) 28, 247
XmiI GTMKAC 2 cut(s) 256, 307
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.