RLG00000022709

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016742
Physical Location & Seq
Forward (+)
10212104 .. 10212628
525 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022709

Sequence Viewer

Length: 525 bp
ATGGCATTGCCTCCACGAAGTTGGAAGTGCTCTCCCCCACTTGCAGTTATCATGCTGCTGATCATGTTGTCCATTTCAGTTCCAGGGAATTCAGCCGCAGTCACTGTCACTTCCGATGAAAAGAACCAACAACAAACAGTGACAGTTTCGTCCACTCAGAAAGAAGAAACCGACACTTCTACTACTACTGGAGAAATCAAGTGTGGAACATGCCCTTGTGTAAACCCTTGTCAACAGCAGCAGCAGCAGATTCCACCACCTCCCCCACCGCCTCCGCCACCAAAGACCACTAGTTGCAGCCCAGTTTTACCTGCAGCCCCAGTATTATTGGCGCCGCCGCCTCCAAGGTTCACTTATGTGACGGGTATTCCAGGTGATTTGTACAAAGCAGATACTTATGGATCGTCCTATTCCTATGATTTTTACTCGGCCGCTGCTCCACCGCCATATCCAAATGTGATCATCACGTTGCCACCTGCCTTGCTTCTCTTCTTGCTCATGGCTTTTGGGAGGATGCTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

18.56

Weight (kDa)

6.12

Isoelectric Point (pI)

57.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29110
malus_domestica MD17G1256500.v1.1
prunus_persica Prupe.3G159100_v2.0.a1
pyrus_communis pycom17g25870
rosa_chinensis RchiOBHm_Chr3g0493521
rosa_laevigata RLG00000022709
rosa_multiflora Rmu_sc0002343.1_g000062
rosa_roxburghii Rroxscaffold_6G00391520
rosa_rugosa Rorug03G0261700
rosa_samantha Rh3AG310600 Rh3BG346800 Rh3CG344600 Rh3DG345900
rosa_wichuraiana Rw3G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 484
AasI GACNNNNNNGTC 1 cut(s) 148
Acc36I ACCTGC 2 cut(s) 319, 484
AccB1I GGYRCC 1 cut(s) 331
AciI CCGC 7 cut(s) 96, 269, 275, 335, 338, 432, 443
AclWI GGATC 1 cut(s) 409
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 1 cut(s) 383
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 2 cut(s) 82, 370
AleI CACNNNNGTG 1 cut(s) 356
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 1 cut(s) 409
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 7 cut(s) 55, 238, 241, 244, 297, 314, 434
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 1 cut(s) 334
AsuHPI GGTGA 1 cut(s) 386
BanI GGYRCC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 7 cut(s) 42, 250, 253, 256, 309, 326, 421
BciT130I CCWGG 2 cut(s) 84, 372
BclI TGATCA 2 cut(s) 60, 459
BcuI ACTAGT 1 cut(s) 290
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 335
BfuAI ACCTGC 2 cut(s) 319, 484
Bme1390I CCNGG 2 cut(s) 84, 372
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 2 cut(s) 84, 372
BmrI ACTGGG 2 cut(s) 296, 314
BmsI GCATC 1 cut(s) 504
BmuI ACTGGG 2 cut(s) 296, 314
BpmI CTGGAG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 83, 344
Bse1I ACTGG 3 cut(s) 193, 302, 320
Bse3DI GCAATG 1 cut(s) 5
BseBI CCWGG 2 cut(s) 84, 372
BseDI CCNNGG 2 cut(s) 83, 344
BseGI GGATG 1 cut(s) 519
BseMI GCAATG 1 cut(s) 5
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 3 cut(s) 193, 302, 320
BseX3I CGGCCG 1 cut(s) 429
BseXI GCAGC 7 cut(s) 42, 250, 253, 256, 309, 326, 421
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 1 cut(s) 431
BshNI GGYRCC 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 32
BsnI GGCC 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 32
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 3 cut(s) 60, 401, 459
BspACI CCGC 7 cut(s) 96, 269, 275, 335, 338, 432, 443
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 333
BspMAI CTGCAG 1 cut(s) 316
BspMI ACCTGC 2 cut(s) 319, 484
BspPI GGATC 1 cut(s) 409
BspT107I GGYRCC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 381
BsrI ACTGG 3 cut(s) 193, 302, 320
BssECI CCNNGG 2 cut(s) 83, 344
BssMI GATC 3 cut(s) 60, 401, 459
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 84, 372
Bst4CI ACNGT 3 cut(s) 106, 139, 145
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 519
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 1 cut(s) 334
BstKTI GATC 3 cut(s) 63, 404, 462
BstMBI GATC 3 cut(s) 60, 401, 459
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 1 cut(s) 244
BstNI CCWGG 2 cut(s) 84, 372
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 2 cut(s) 82, 370
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 7 cut(s) 42, 250, 253, 256, 309, 326, 421
BstXI CCANNNNNNTGG 1 cut(s) 21
BstZI CGGCCG 1 cut(s) 429
BsuRI GGCC 1 cut(s) 431
BtsCI GGATG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 102, 144
BveI ACCTGC 2 cut(s) 319, 484
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 1 cut(s) 334
Csp6I GTAC 1 cut(s) 382
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviAII CATG 4 cut(s) 52, 64, 210, 499
CviJI RGCY 5 cut(s) 95, 300, 317, 431, 503
CviKI_1 RGCY 5 cut(s) 95, 300, 317, 431, 503
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 1 cut(s) 156
DinI GGCGCC 1 cut(s) 333
DpnI GATC 3 cut(s) 62, 403, 461
DpnII GATC 3 cut(s) 60, 401, 459
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 429
EagI CGGCCG 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 1 cut(s) 264
EclXI CGGCCG 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 344
Eco52I CGGCCG 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 2 cut(s) 82, 370
EcoT14I CCWWGG 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 333
EheI GGCGCC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 344
FaeI CATG 4 cut(s) 55, 67, 213, 502
FaiI YATR 8 cut(s) 53, 65, 211, 357, 399, 417, 448, 500
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FatI CATG 4 cut(s) 51, 63, 209, 498
FbaI TGATCA 2 cut(s) 60, 459
FspBI CTAG 1 cut(s) 291
GlaI GCGC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 1 cut(s) 431
HhaI GCGC 1 cut(s) 334
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 4 cut(s) 55, 67, 213, 502
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HincII GTYRAC 1 cut(s) 233
HindII GTYRAC 1 cut(s) 233
HinfI GANTC 1 cut(s) 250
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 4 cut(s) 153, 223, 233, 351
Hpy188I TCNGA 2 cut(s) 115, 159
Hpy8I GTNNAC 4 cut(s) 153, 223, 233, 351
HpyCH4III ACNGT 3 cut(s) 106, 139, 145
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 3 cut(s) 44, 297, 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 244
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 467
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 4 cut(s) 55, 67, 213, 502
HspAI GCGC 1 cut(s) 332
KasI GGCGCC 1 cut(s) 331
Ksp22I TGATCA 2 cut(s) 60, 459
Kzo9I GATC 3 cut(s) 60, 401, 459
LmnI GCTCC 1 cut(s) 442
LpnPI CCDG 9 cut(s) 69, 96, 174, 315, 324, 333, 357, 384, 489
Lsp1109I GCAGC 7 cut(s) 42, 250, 253, 256, 309, 326, 421
LweI GCATC 1 cut(s) 504
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 4 cut(s) 100, 106, 139, 358
MalI GATC 3 cut(s) 62, 403, 461
MboI GATC 3 cut(s) 60, 401, 459
MboII GAAGA 2 cut(s) 176, 481
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 1 cut(s) 88
Mly113I GGCGCC 1 cut(s) 332
MnlI CCTC 5 cut(s) 21, 270, 282, 351, 504
MslI CAYNNNNRTG 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 434
MspR9I CCNGG 2 cut(s) 84, 372
MvaI CCWGG 2 cut(s) 84, 372
MwoI GCNNNNNNNGC 1 cut(s) 244
NarI GGCGCC 1 cut(s) 332
NdeII GATC 3 cut(s) 60, 401, 459
NlaIII CATG 4 cut(s) 55, 67, 213, 502
NlaIV GGNNCC 1 cut(s) 333
NmeAIII GCCGAG 1 cut(s) 407
NmuCI GTSAC 4 cut(s) 100, 106, 139, 358
NspI RCATGY 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 356
PaqCI CACCTGC 1 cut(s) 484
PfeI GAWTC 1 cut(s) 250
PluTI GGCGCC 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 82, 370
PspGI CCWGG 2 cut(s) 82, 370
PspN4I GGNNCC 1 cut(s) 333
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 104
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 356
Sau3AI GATC 3 cut(s) 60, 401, 459
ScrFI CCNGG 2 cut(s) 84, 372
SduI GDGCHC 1 cut(s) 32
SetI ASST 6 cut(s) 262, 313, 350, 376, 470, 478
SfaNI GCATC 1 cut(s) 504
SfcI CTRYAG 1 cut(s) 312
SfoI GGCGCC 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 356
SpeI ACTAGT 1 cut(s) 290
Sse9I AATT 1 cut(s) 88
SsiI CCGC 7 cut(s) 96, 269, 275, 335, 338, 432, 443
SspDI GGCGCC 1 cut(s) 331
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 82, 370
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 3 cut(s) 106, 139, 145
TaiI ACGT 1 cut(s) 470
TasI AATT 1 cut(s) 88
TatI WGTACW 1 cut(s) 381
TauI GCSGC 4 cut(s) 98, 337, 340, 434
TfiI GAWTC 1 cut(s) 250
TscAI CASTG 2 cut(s) 109, 144
TseFI GTSAC 4 cut(s) 100, 106, 139, 358
TseI GCWGC 7 cut(s) 55, 238, 241, 244, 297, 314, 434
Tsp45I GTSAC 4 cut(s) 100, 106, 139, 358
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 2 cut(s) 109, 144
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 213
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.