Rw3G027300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0016742
Physical Location & Seq
Reverse (-)
38502040 .. 38503203
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027300.1

Sequence Viewer

Length: 519 bp
ATGGCATTGCCTCCACGAAGTTGGAAGTGCTCTCCCCCACTTGCAGTTATCATGCTGCTGATCATTTTGTCCATTTCAGTTCCAGAGAATTCAGCCGCAATCACTGTCACTTCCGATGAAAAGAACCAACAACAAACAGTGACGGTTTCGTCCACTCAGAAAGAAGAAACCGACACTTCTACTACTACTGGAGAAATCAAGTGTGGAACATGCCCTTGTGTAAACCCTTGTCAACAGCAGCAGATTCCACCACCTCCCCCACCGCCTCCGCCACCAAAGACCACTAGTTGCAGCCCAGTTTTACCTACAGCCCCAGTATTATTGGCGCCGCCGCCTCCAAGGTTCATTTATGTGACGGGTATTCCAGGTGATTTGTACAAAACAGATACTTATGGATCGTCCTATTCCTATGATTTTTACTCGGCCGCTGCTCCATCGCCATATCCAAATGTGATCATCACGTTGCCACCTGCCTTGCTCCTCGTCTTGCTCATGGCTTTTGGGAGGTTGCTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

18.36

Weight (kDa)

5.24

Isoelectric Point (pI)

59.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016742)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29110
malus_domestica MD17G1256500.v1.1
prunus_persica Prupe.3G159100_v2.0.a1
pyrus_communis pycom17g25870
rosa_chinensis RchiOBHm_Chr3g0493521
rosa_laevigata RLG00000022709
rosa_multiflora Rmu_sc0002343.1_g000062
rosa_roxburghii Rroxscaffold_6G00391520
rosa_rugosa Rorug03G0261700
rosa_samantha Rh3AG310600 Rh3BG346800 Rh3CG344600 Rh3DG345900
rosa_wichuraiana Rw3G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
AasI GACNNNNNNGTC 1 cut(s) 148
Acc36I ACCTGC 1 cut(s) 478
AccB1I GGYRCC 1 cut(s) 325
AciI CCGC 6 cut(s) 96, 263, 269, 329, 332, 426
AclWI GGATC 1 cut(s) 403
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 1 cut(s) 377
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 1 cut(s) 364
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 1 cut(s) 403
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 4 cut(s) 55, 238, 291, 428
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 380
BanI GGYRCC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 4 cut(s) 42, 250, 303, 415
BccI CCATC 1 cut(s) 442
BciT130I CCWGG 1 cut(s) 366
BclI TGATCA 2 cut(s) 60, 453
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 478
BisI GCNGC 8 cut(s) 56, 96, 239, 292, 329, 332, 426, 429
BlsI GCNGC 8 cut(s) 57, 97, 240, 293, 330, 333, 427, 430
Bme1390I CCNGG 1 cut(s) 366
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 2 cut(s) 290, 308
BmuI ACTGGG 2 cut(s) 290, 308
BpmI CTGGAG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 193, 296, 314
Bse3DI GCAATG 1 cut(s) 5
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 1 cut(s) 338
BseMI GCAATG 1 cut(s) 5
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 3 cut(s) 193, 296, 314
BseRI GAGGAG 1 cut(s) 470
BseX3I CGGCCG 1 cut(s) 423
BseXI GCAGC 4 cut(s) 42, 250, 303, 415
Bsh1285I CGRYCG 1 cut(s) 426
BshFI GGCC 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 32
BsnI GGCC 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 32
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 60, 395, 453
BspACI CCGC 6 cut(s) 96, 263, 269, 329, 332, 426
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 327
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 3 cut(s) 193, 296, 314
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 3 cut(s) 60, 395, 453
BssNI GRCGYC 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 3 cut(s) 106, 139, 145
BstACI GRCGYC 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 375
BstDEI CTNAG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 63, 398, 456
BstMBI GATC 3 cut(s) 60, 395, 453
BstMCI CGRYCG 1 cut(s) 426
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 4 cut(s) 42, 250, 303, 415
BstXI CCANNNNNNTGG 1 cut(s) 21
BstZI CGGCCG 1 cut(s) 423
BsuRI GGCC 1 cut(s) 425
BtgZI GCGATG 1 cut(s) 420
BtsIMutI CAGTG 2 cut(s) 102, 144
BveI ACCTGC 1 cut(s) 478
CfoI GCGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 3 cut(s) 52, 210, 493
CviJI RGCY 5 cut(s) 95, 294, 311, 425, 497
CviKI_1 RGCY 5 cut(s) 95, 294, 311, 425, 497
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 1 cut(s) 156
DinI GGCGCC 1 cut(s) 327
DpnI GATC 3 cut(s) 62, 397, 455
DpnII GATC 3 cut(s) 60, 395, 453
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 423
EagI CGGCCG 1 cut(s) 423
EciI GGCGGA 1 cut(s) 258
EclXI CGGCCG 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 338
Eco52I CGGCCG 1 cut(s) 423
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 338
EgeI GGCGCC 1 cut(s) 327
EheI GGCGCC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 3 cut(s) 55, 213, 496
FaiI YATR 7 cut(s) 53, 211, 351, 393, 411, 442, 494
FatI CATG 3 cut(s) 51, 209, 492
FbaI TGATCA 2 cut(s) 60, 453
Fnu4HI GCNGC 8 cut(s) 56, 96, 239, 292, 329, 332, 426, 429
Fsp4HI GCNGC 8 cut(s) 56, 96, 239, 292, 329, 332, 426, 429
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 327
GluI GCNGC 8 cut(s) 56, 96, 239, 292, 329, 332, 426, 429
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 1 cut(s) 425
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 3 cut(s) 55, 213, 496
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 233
HindII GTYRAC 1 cut(s) 233
HinfI GANTC 1 cut(s) 244
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 3 cut(s) 153, 223, 233
Hpy188I TCNGA 2 cut(s) 115, 159
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 3 cut(s) 153, 223, 233
HpyCH4III ACNGT 3 cut(s) 106, 139, 145
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 2 cut(s) 44, 291
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 461
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 3 cut(s) 55, 213, 496
HspAI GCGC 1 cut(s) 326
KasI GGCGCC 1 cut(s) 325
Ksp22I TGATCA 2 cut(s) 60, 453
Kzo9I GATC 3 cut(s) 60, 395, 453
LmnI GCTCC 2 cut(s) 436, 483
LpnPI CCDG 7 cut(s) 96, 174, 309, 327, 351, 378, 483
Lsp1109I GCAGC 4 cut(s) 42, 250, 303, 415
MaeI CTAG 1 cut(s) 285
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 3 cut(s) 106, 139, 352
MalI GATC 3 cut(s) 62, 397, 455
MboI GATC 3 cut(s) 60, 395, 453
MboII GAAGA 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 1 cut(s) 88
Mly113I GGCGCC 1 cut(s) 326
MnlI CCTC 6 cut(s) 21, 264, 276, 345, 491, 498
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 366
NarI GGCGCC 1 cut(s) 326
NdeII GATC 3 cut(s) 60, 395, 453
NlaIII CATG 3 cut(s) 55, 213, 496
NlaIV GGNNCC 1 cut(s) 327
NmeAIII GCCGAG 1 cut(s) 401
NmuCI GTSAC 3 cut(s) 106, 139, 352
NspI RCATGY 1 cut(s) 213
PaqCI CACCTGC 1 cut(s) 478
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 8 cut(s) 57, 97, 240, 293, 330, 333, 427, 430
PluTI GGCGCC 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 327
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 350
SatI GCNGC 8 cut(s) 56, 96, 239, 292, 329, 332, 426, 429
Sau3AI GATC 3 cut(s) 60, 395, 453
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 1 cut(s) 32
SetI ASST 7 cut(s) 256, 307, 344, 370, 464, 472, 509
SfcI CTRYAG 1 cut(s) 306
SfoI GGCGCC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 350
SpeI ACTAGT 1 cut(s) 284
Sse9I AATT 1 cut(s) 88
SsiI CCGC 6 cut(s) 96, 263, 269, 329, 332, 426
SspDI GGCGCC 1 cut(s) 325
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 3 cut(s) 106, 139, 145
TaiI ACGT 1 cut(s) 464
TasI AATT 1 cut(s) 88
TatI WGTACW 1 cut(s) 375
TauI GCSGC 4 cut(s) 98, 331, 334, 428
TfiI GAWTC 1 cut(s) 244
TscAI CASTG 2 cut(s) 109, 144
TseFI GTSAC 3 cut(s) 106, 139, 352
TseI GCWGC 4 cut(s) 55, 238, 291, 428
Tsp45I GTSAC 3 cut(s) 106, 139, 352
TspDTI ATGAA 2 cut(s) 132, 334
TspRI CASTG 2 cut(s) 109, 144
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 213
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.