Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012762

Physical Location & Seq

Genomic Coordinates Reverse (-)

32313444 .. 32318342

Gene Structure 4899 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1262700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2859 bp

ATGAAGAAGAAAACCCATGCTGGGTTTAAGTTTTTGACCATTTTGCTTGCTGGCCTTTCATCAATCTTGCTGGGTGCCAACTCCCAACCGAGTTCAAGCAACGATGCTGCAGTGATGCTGGCTCTGAAGAAAACCCTCAAACCATCTGAATCTGTCGGATGGTCCGACCCGGACCCCTGCCAATGGACCCATGTGGGCTGCTCAGACGACAAGAGGGTGACCCGAATCCAACTGGGTCACCTTAATATAGAAGGTACGCTTCCTTCTAGCCTCCAAAATCTCACTGAACTCGAGCGTTTAGAGCTTCAATGGAACAAAATTTCAGGTCCTCTTCCGAGCCTTAGTGGGTTAAGTTCACTGCAAGTGTTACTGATCAGTAACAATTTGTTTACTTCAATTCCCAGTGATTTTTTTACTGGTATGACTTCCCTTCAATCTGTTGAGATTGATAACAACCCTTTTATGAGTTGGGAAATTCCTGAAACTCTCAAGAATGCATCTTCACTTCAAAACTTTTCGGCCAATTCGGCCAACATTACTGGGAAAATACCCTATTTTTTCAATGGAGATGCGTTTCCGAGTCTTGTTAACTTGCACCTGGCCTTCAATGGCCTCGAAGGCGACCTGCCCACGAGCTTTTCTGGGTTGCAAATTCAGTCTCTGTGGCTTAATGGGCAAGAAAGTGTAGGTAAGCTTGGTGGAAGCATTGCTGTTTTACAGAACATGACTCTGTTGAAGGAGGTTTGGTTGCATTCGAATGCGTTTTCAGGTCCATTACCAGACTTTTCGGGTTTAACAGACTTGCGGAGCTTGAGTTTGAGAGATAATATGTTTACTGGTCCTGTTCCAGTGTCATTGGTGAGCCTTAAGTCACTAGAGGCTGTGAACTTGACAAACAATTTGCTTCAAGGACCAATGCCGGCTTTTGTTTCAGGGGTTGCAGTAGATTTGGTTAAGGGTTCTAATAACTTTTGCTTGCCGAGTCCTGGTCAATGTGATCCACGAGTGAATACTTTGCTTTCGATTGCGAGTTCAGTGGGTTACCCCCAAAAGTTTGCAGAGAATTGGAAGGGGAATGATCCATGTGCAGATTGGATTGGGGTTACTTGTAGTAATGGGAACATTACTGTCCTCAATTTTCAGAAAATGGGTCTTAATGGAACGATTTCTCCCGAAATCGCGTCATTGAAGTCCCTTCAAAGGTTAGTTCTTGCTGCTAACAATTTAACTGGAACGATTCCGGCGGAGCTTGCCACGCTGCCTGCACTCACTGTATTTGATGTGTCAAACAATAATCTCTATGGGAAACCCCCAGCTTTTAATGATAAGGTGCTTGTAAAAATGTTTGGTAACCCTGATATAGGGAACGAAAAGAGTACTTCAGTGGGGGGTGCACCGTCTCAAAATTCCACCAACCCTTCTACAAGTATAGGTTCTGGAAACAGTTCTAGACCTCATGGAAAGAAATCTTCAACTCTAATAGGAGTCATTGTGTTCTCTGTAATTGGGGGTGTATTTGTGATTTTATTAATTGCACTGTTGCTTATCTGTGTATATAGAACAAAACAAAAGCAGTTGAGCAGAGTTCAGAGCCCGAATGCAATGGTAGTTCATCCCCGCCACTCTGGATCCGATAATGAGAACATGAAGATCACAGTTGCAGGCTCTAGTGTTAGTGTTGGTGCACTAAGTGAAGCTCATACTCTTCCTAGCAGTGAGTCCAATGAAATCCAAATGGTTGACGCAGGAAACATGGTGATTTCTATTCAAGTGCTCCGGAACGTGACAAACAATTTCAGTCAAGAAAATATATTGGGACAAGGAGGTTTTGGGACAGTGTATAAAGGGGAACTGCACGATGGAACAAAGATTGCTGTTAAGAGAATGGAGGCTGGGGTAATAGTAGGCAAGGGGCTTTCTGAATTTAAGTCTGAGATTTCTGTTTTGACAAAGGTTCGTCACAGGCATCTCGTAGCTCTTCTGGGGTATTGTTTAGATGGAAATGAAAGGCTTCTTGTATATGAGTATATGCCTCAAGGAACGCTCAGCAGGCACCTCTTCAACTGGCCGGAAGAAGGACTGAAACCACTGGAATGGACAAGAAGGCTGACAATTGCCTTAGATGTAGCAAGGGGTGTTGAGTATCTCCATGGTTTGGCCCATCAGAGTTTTATACATAGGGACTTAAAGCCTTCAAATATTCTTCTTGGTGATGATATGAGAGCTAAGGTTGCAGATTTCGGCCTTGTACGGCTTGCTCCAGAGGGCAAAGGTTCAATTGAAACAAGGATTGCAGGGACTTTTGGATACTTGGCACCAGAATATGCAGTTACTGGTCGTGTGACAACGAAAGTAGATGTGTTCAGTTTTGGAGTGATTTTGATGGAGCTGATCACGGGGAGAAAGGCACTTGACGACAGCCAGCCTGAGGAGAGCATGCACTTAGTGACATGGTTCCGGAGAATGTTCATTAACAAGGACTCATTCCGCAAGGCAATTGATCCCACAATTGATCTCAACGAGGAAACACTTGCCAGTGTCAGCACTGTCGCTGAGTTAGCAGGTCACTGCTGTGCCAGAGAGCCGTATCAGAGGCCTGACATGGGCCACATCGTGAATGTCCTATCTTCTCTGGTGGAGCTTTGGAAGCCAAGCGATCAAAGTTCCGAAGACATATATGGTATAGACCTTGAAATGTCGTTGCCGCAAGCACTTAAGAAGTGGCAGGCATATGAAGGTAGAAGCAATTCGGAATCCTCTTCTTCATCGCTTCTTCCCAGCTTGGATAACACTCAGACGAGCATCCCTACACGCCCGTATGGATTTGCCGAGTCTTTCACATCTGCAGATGGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

953

Amino Acids

103.11

Weight (kDa)

6.21

Isoelectric Point (pI)

39.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	33 - 68	5.1e-06	Leucine rich repeat N-terminal domain
LRRNT_2	PF08263	336 - 371	1.1e-06	Leucine rich repeat N-terminal domain
Pkinase	PF00069	600 - 873	3.5e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	601 - 874	7e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66150
fragaria_vesca	FvH4_1g29920
malus_domestica	MD09G1266700.v1.1 MD17G1262700.v1.1
prunus_persica	Prupe.3G164300_v2.0.a1
pyrus_communis	pycom09g17970 pycom17g26270
rosa_chinensis	RchiOBHm_Chr3g0492431
rosa_laevigata	RLG00000022769
rosa_multiflora	Rmu_sc0003156.1_g000009 Rmu_sc0027181.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392320
rosa_rugosa	Rorug03G0253800
rosa_samantha	Rh3AG304000 Rh3BG339900 Rh3CG337100 Rh3DG339100
rosa_wichuraiana	Rw3G026800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	633, 2552
AccB1I	GGYRCC	3 cut(s)	74, 2052, 2314
AccB7I	CCANNNNNTGG	1 cut(s)	2155
AccII	CGCG	1 cut(s)	1181
AccIII	TCCGGA	2 cut(s)	1776, 2457
AciI	CCGC	5 cut(s)	805, 1244, 1618, 2488, 2705
AclWI	GGATC	5 cut(s)	992, 1073, 1623, 1636, 2495
AcoI	YGGCCR	3 cut(s)	519, 528, 2066
AcsI	RAATTY	5 cut(s)	318, 474, 651, 1405, 1922
AcuI	CTGAAG	2 cut(s)	146, 1365
AdeI	CACNNNGTG	3 cut(s)	1691, 2446, 2614
AfaI	GTAC	3 cut(s)	256, 1378, 2250
AfiI	CCNNNNNNNGG	9 cut(s)	21, 183, 986, 1200, 1361, 2075, 2155, 2428, 2603
AflII	CTTAAG	2 cut(s)	866, 2714
AjnI	CCWGG	2 cut(s)	597, 985
AleI	CACNNNNGTG	1 cut(s)	2571
AloI	GAACNNNNNNTCC	2 cut(s)	1153, 1185
Alw21I	GWGCWC	3 cut(s)	1396, 1687, 1776
Alw26I	GTCTC	2 cut(s)	663, 1404
Alw44I	GTGCAC	2 cut(s)	1392, 1683
AlwI	GGATC	5 cut(s)	992, 1073, 1623, 1636, 2495
AlwNI	CAGNNNCTG	2 cut(s)	661, 2333
Ama87I	CYCGRG	1 cut(s)	290
Aor13HI	TCCGGA	2 cut(s)	1776, 2457
ApaLI	GTGCAC	2 cut(s)	1392, 1683
ApeKI	GCWGC	4 cut(s)	107, 198, 1214, 1258
ApoI	RAATTY	5 cut(s)	318, 474, 651, 1405, 1922
ArsI	GACNNNNNNTTYG	2 cut(s)	883, 915
AseI	ATTAAT	1 cut(s)	1529
Asp700I	GAANNNNTTC	4 cut(s)	1165, 1444, 2010, 2746
AspS9I	GGNCC	9 cut(s)	162, 172, 186, 326, 770, 839, 911, 2158, 2605
AsuC2I	CCSGG	1 cut(s)	170
AsuHPI	GGTGA	5 cut(s)	229, 230, 871, 1768, 2222
AsuII	TTCGAA	1 cut(s)	755
AvaI	CYCGRG	1 cut(s)	290
AvaII	GGWCC	7 cut(s)	162, 172, 186, 326, 770, 839, 911
AxyI	CCTNAGG	1 cut(s)	2427
BaeGI	GKGCMC	2 cut(s)	1396, 1687
BamHI	GGATCC	1 cut(s)	1628
BanI	GGYRCC	3 cut(s)	74, 2052, 2314
BanII	GRGCYC	1 cut(s)	1595
BarI	GAAGNNNNNNTAC	2 cut(s)	247, 279
BauI	CACGAG	2 cut(s)	631, 1002
BbsI	GAAGAC	1 cut(s)	2676
Bbv12I	GWGCWC	3 cut(s)	1396, 1687, 1776
BbvI	GCAGC	4 cut(s)	94, 185, 1201, 1245
BccI	CCATC	7 cut(s)	151, 153, 1853, 1991, 2169, 2377, 2843
BceAI	ACGGC	2 cut(s)	2267, 2569
BciT130I	CCWGG	2 cut(s)	599, 987
BciVI	GTATCC	1 cut(s)	2300
BclI	TGATCA	2 cut(s)	372, 2391
BcnI	CCSGG	1 cut(s)	170
BcoDI	GTCTC	2 cut(s)	663, 1404
BfaI	CTAG	5 cut(s)	267, 875, 1449, 1668, 1710
BfmI	CTRYAG	2 cut(s)	108, 2844
BfrI	CTTAAG	2 cut(s)	866, 2714
BfuAI	ACCTGC	2 cut(s)	633, 2552
BfuI	GTATCC	1 cut(s)	2300
BglI	GCCNNNNNGGC	2 cut(s)	527, 618
BisI	GCNGC	5 cut(s)	108, 199, 1215, 1259, 2705
BlpI	GCTNAGC	1 cut(s)	2045
BlsI	GCNGC	5 cut(s)	109, 200, 1216, 1260, 2706
BmcAI	AGTACT	1 cut(s)	1378
Bme1390I	CCNGG	3 cut(s)	170, 599, 987
Bme18I	GGWCC	7 cut(s)	162, 172, 186, 326, 770, 839, 911
BmeT110I	CYCGRG	1 cut(s)	290
BmgT120I	GGNCC	9 cut(s)	162, 172, 186, 326, 770, 839, 911, 2158, 2605
BmiI	GGNNCC	7 cut(s)	76, 174, 188, 1630, 2054, 2316, 2456
BmrFI	CCNGG	3 cut(s)	170, 599, 987
BmrI	ACTGGG	3 cut(s)	242, 396, 549
BmsI	GCATC	6 cut(s)	94, 105, 506, 559, 1975, 2811
BmuI	ACTGGG	3 cut(s)	242, 396, 549
BpiI	GAAGAC	1 cut(s)	2676
BpmI	CTGGAG	1 cut(s)	2244
Bpu10I	CCTNAGC	1 cut(s)	2226
Bpu1102I	GCTNAGC	1 cut(s)	2045
Bpu14I	TTCGAA	1 cut(s)	755
BpuEI	CTTGAG	3 cut(s)	473, 832, 2019
BpuMI	CCSGG	1 cut(s)	170
BsaJI	CCNNGG	1 cut(s)	2149
BsaWI	WCCGGW	2 cut(s)	1776, 2457
BsaXI	ACNNNNNCTCC	8 cut(s)	731, 761, 799, 829, 1153, 1183, 1476, 1506
Bsc4I	CCNNNNNNNGG	9 cut(s)	21, 183, 986, 1200, 1361, 2075, 2155, 2428, 2603
Bse118I	RCCGGY	1 cut(s)	919
Bse21I	CCTNAGG	1 cut(s)	2427
Bse3DI	GCAATG	2 cut(s)	705, 1608
BseAI	TCCGGA	2 cut(s)	1776, 2457
BseBI	CCWGG	2 cut(s)	599, 987
BseDI	CCNNGG	1 cut(s)	2149
BseGI	GGATG	3 cut(s)	164, 1612, 2802
BseLI	CCNNNNNNNGG	9 cut(s)	21, 183, 986, 1200, 1361, 2075, 2155, 2428, 2603
BseMI	GCAATG	2 cut(s)	705, 1608
BseMII	CTCAG	6 cut(s)	216, 1923, 2059, 2418, 2544, 2807
BseRI	GAGGAG	1 cut(s)	2444
BseSI	GKGCMC	2 cut(s)	1396, 1687
BseXI	GCAGC	4 cut(s)	94, 185, 1201, 1245
BseYI	CCCAGC	5 cut(s)	20, 70, 1312, 1892, 2777
BsgI	GTGCAG	3 cut(s)	1107, 1248, 1838
Bsh1236I	CGCG	1 cut(s)	1181
BshNI	GGYRCC	3 cut(s)	74, 2052, 2314
BsiHKAI	GWGCWC	3 cut(s)	1396, 1687, 1776
BsiHKCI	CYCGRG	1 cut(s)	290
BsiSI	CCGG	6 cut(s)	170, 920, 1241, 1777, 2069, 2458
BslFI	GGGAC	5 cut(s)	1177, 1830, 1846, 2195, 2311
BslI	CCNNNNNNNGG	9 cut(s)	21, 183, 986, 1200, 1361, 2075, 2155, 2428, 2603
BsmAI	GTCTC	2 cut(s)	663, 1404
BsmBI	CGTCTC	1 cut(s)	1404
BsmFI	GGGAC	5 cut(s)	1177, 1830, 1846, 2195, 2311
BsmI	GAATGC	4 cut(s)	499, 751, 763, 1603
BsoBI	CYCGRG	1 cut(s)	290
Bsp119I	TTCGAA	1 cut(s)	755
Bsp1286I	GDGCHC	4 cut(s)	1396, 1595, 1687, 1776
Bsp13I	TCCGGA	2 cut(s)	1776, 2457
Bsp143I	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
Bsp1720I	GCTNAGC	1 cut(s)	2045
Bsp19I	CCATGG	1 cut(s)	2149
BspACI	CCGC	5 cut(s)	805, 1244, 1618, 2488, 2705
BspCNI	CTCAG	6 cut(s)	215, 1924, 2058, 2419, 2545, 2806
BspEI	TCCGGA	2 cut(s)	1776, 2457
BspFNI	CGCG	1 cut(s)	1181
BspLI	GGNNCC	7 cut(s)	76, 174, 188, 1630, 2054, 2316, 2456
BspMAI	CTGCAG	2 cut(s)	112, 2848
BspMI	ACCTGC	2 cut(s)	633, 2552
BspPI	GGATC	5 cut(s)	992, 1073, 1623, 1636, 2495
BspQI	GCTCTTC	1 cut(s)	1983
BspT104I	TTCGAA	1 cut(s)	755
BspT107I	GGYRCC	3 cut(s)	74, 2052, 2314
BspTI	CTTAAG	2 cut(s)	866, 2714
BsrDI	GCAATG	2 cut(s)	705, 1608
BsrFI	RCCGGY	1 cut(s)	919
BssAI	RCCGGY	1 cut(s)	919
BssECI	CCNNGG	1 cut(s)	2149
BssMI	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
BssSI	CACGAG	2 cut(s)	631, 1002
BssT1I	CCWWGG	1 cut(s)	2149
Bst2BI	CACGAG	2 cut(s)	631, 1002
Bst2UI	CCWGG	2 cut(s)	599, 987
Bst4CI	ACNGT	8 cut(s)	1129, 1273, 1398, 1445, 1539, 1657, 1837, 2548
Bst6I	CTCTTC	5 cut(s)	336, 1710, 1983, 2063, 2764
BstAFI	CTTAAG	2 cut(s)	866, 2714
BstBI	TTCGAA	1 cut(s)	755
BstDSI	CCRYGG	1 cut(s)	2149
BstEII	GGTNACC	4 cut(s)	217, 236, 1040, 1349
BstENI	CCTNNNNNAGG	1 cut(s)	1359
BstF5I	GGATG	3 cut(s)	164, 1612, 2802
BstFNI	CGCG	1 cut(s)	1181
BstKTI	GATC	9 cut(s)	375, 1000, 1081, 1631, 1653, 2394, 2503, 2515, 2659
BstMAI	GTCTC	2 cut(s)	663, 1404
BstMBI	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
BstNI	CCWGG	2 cut(s)	599, 987
BstNSI	RCATGY	1 cut(s)	2440
BstPI	GGTNACC	4 cut(s)	217, 236, 1040, 1349
BstSCI	CCNGG	3 cut(s)	168, 597, 985
BstSFI	CTRYAG	2 cut(s)	108, 2844
BstSLI	GKGCMC	2 cut(s)	1396, 1687
BstUI	CGCG	1 cut(s)	1181
BstV1I	GCAGC	4 cut(s)	94, 185, 1201, 1245
BstV2I	GAAGAC	1 cut(s)	2676
BstX2I	RGATCY	1 cut(s)	1628
BstXI	CCANNNNNNTGG	1 cut(s)	2094
BstYI	RGATCY	1 cut(s)	1628
Bsu36I	CCTNAGG	1 cut(s)	2427
BsuI	GTATCC	1 cut(s)	2300
BtgI	CCRYGG	1 cut(s)	2149
BtgZI	GCGATG	1 cut(s)	2751
BtsCI	GGATG	3 cut(s)	164, 1612, 2802
BtsI	GCAGTG	4 cut(s)	117, 356, 1720, 2566
BveI	ACCTGC	2 cut(s)	633, 2552
CaiI	CAGNNNCTG	2 cut(s)	661, 2333
Cfr10I	RCCGGY	1 cut(s)	919
Cfr13I	GGNCC	9 cut(s)	162, 172, 186, 326, 770, 839, 911, 2158, 2605
CseI	GACGC	2 cut(s)	1170, 1751
Csp6I	GTAC	3 cut(s)	255, 1377, 2249
CviQI	GTAC	3 cut(s)	255, 1377, 2249
DpnI	GATC	9 cut(s)	374, 999, 1080, 1630, 1652, 2393, 2502, 2514, 2658
DpnII	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
DraIII	CACNNNGTG	3 cut(s)	1691, 2446, 2614
EaeI	YGGCCR	3 cut(s)	519, 528, 2066
Eam1104I	CTCTTC	5 cut(s)	336, 1710, 1983, 2063, 2764
EarI	CTCTTC	5 cut(s)	336, 1710, 1983, 2063, 2764
EciI	GGCGGA	1 cut(s)	1259
Eco130I	CCWWGG	1 cut(s)	2149
Eco147I	AGGCCT	1 cut(s)	2596
Eco24I	GRGCYC	1 cut(s)	1595
Eco47I	GGWCC	7 cut(s)	162, 172, 186, 326, 770, 839, 911
Eco57I	CTGAAG	2 cut(s)	146, 1365
Eco81I	CCTNAGG	1 cut(s)	2427
Eco88I	CYCGRG	1 cut(s)	290
Eco91I	GGTNACC	4 cut(s)	217, 236, 1040, 1349
EcoNI	CCTNNNNNAGG	1 cut(s)	1359
EcoO109I	RGGNCCY	1 cut(s)	326
EcoO65I	GGTNACC	4 cut(s)	217, 236, 1040, 1349
EcoRII	CCWGG	2 cut(s)	597, 985
EcoT14I	CCWWGG	1 cut(s)	2149
EcoT22I	ATGCAT	1 cut(s)	499
EcoT38I	GRGCYC	1 cut(s)	1595
ErhI	CCWWGG	1 cut(s)	2149
Esp3I	CGTCTC	1 cut(s)	1404
FalI	AAGNNNNNCTT	2 cut(s)	243, 275
FaqI	GGGAC	5 cut(s)	1177, 1830, 1846, 2195, 2311
FauI	CCCGC	1 cut(s)	1625
FauNDI	CATATG	1 cut(s)	2731
FbaI	TGATCA	2 cut(s)	372, 2391
Fnu4HI	GCNGC	5 cut(s)	108, 199, 1215, 1259, 2705
FokI	GGATG	3 cut(s)	171, 1599, 2789
FriOI	GRGCYC	1 cut(s)	1595
Fsp4HI	GCNGC	5 cut(s)	108, 199, 1215, 1259, 2705
FspBI	CTAG	5 cut(s)	267, 875, 1449, 1668, 1710
GluI	GCNGC	5 cut(s)	108, 199, 1215, 1259, 2705
GsaI	CCCAGC	5 cut(s)	24, 74, 1316, 1896, 2781
GsuI	CTGGAG	1 cut(s)	2244
HapII	CCGG	6 cut(s)	170, 920, 1241, 1777, 2069, 2458
HgaI	GACGC	2 cut(s)	1170, 1751
HincII	GTYRAC	2 cut(s)	589, 1741
HindII	GTYRAC	2 cut(s)	589, 1741
HindIII	AAGCTT	1 cut(s)	692
HpaI	GTTAAC	1 cut(s)	589
HpaII	CCGG	6 cut(s)	170, 920, 1241, 1777, 2069, 2458
HphI	GGTGA	5 cut(s)	229, 230, 871, 1768, 2222
Hpy166II	GTNNAC	8 cut(s)	356, 390, 589, 834, 886, 1394, 1685, 1741
Hpy8I	GTNNAC	8 cut(s)	356, 390, 589, 834, 886, 1394, 1685, 1741
HpyCH4III	ACNGT	8 cut(s)	1129, 1273, 1398, 1445, 1539, 1657, 1837, 2548
HpyCH4IV	ACGT	1 cut(s)	1782
HpySE526I	ACGT	1 cut(s)	1782
Kpn2I	TCCGGA	2 cut(s)	1776, 2457
KroI	GCCGGC	1 cut(s)	919
KroNI	GCCGGC	1 cut(s)	921
Ksp22I	TGATCA	2 cut(s)	372, 2391
KspAI	GTTAAC	1 cut(s)	589
Kzo9I	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
LguI	GCTCTTC	1 cut(s)	1983
LmnI	GCTCC	6 cut(s)	807, 1246, 1779, 2263, 2386, 2638
Lsp1109I	GCAGC	4 cut(s)	94, 185, 1201, 1245
LweI	GCATC	6 cut(s)	94, 105, 506, 559, 1975, 2811
MaeI	CTAG	5 cut(s)	267, 875, 1449, 1668, 1710
MaeII	ACGT	1 cut(s)	1782
MalI	GATC	9 cut(s)	374, 999, 1080, 1630, 1652, 2393, 2502, 2514, 2658
MboI	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
MfeI	CAATTG	4 cut(s)	2112, 2277, 2496, 2508
MflI	RGATCY	1 cut(s)	1628
MhlI	GDGCHC	4 cut(s)	1396, 1595, 1687, 1776
MlyI	GAGTC	7 cut(s)	589, 721, 991, 1494, 1727, 2474, 2840
MmeI	TCCRAC	3 cut(s)	136, 189, 253
Mph1103I	ATGCAT	1 cut(s)	499
MroI	TCCGGA	2 cut(s)	1776, 2457
MroNI	GCCGGC	1 cut(s)	919
MroXI	GAANNNNTTC	4 cut(s)	1165, 1444, 2010, 2746
MslI	CAYNNNNRTG	3 cut(s)	756, 2092, 2571
MspCI	CTTAAG	2 cut(s)	866, 2714
MspI	CCGG	6 cut(s)	170, 920, 1241, 1777, 2069, 2458
MspR9I	CCNGG	3 cut(s)	170, 599, 987
MunI	CAATTG	4 cut(s)	2112, 2277, 2496, 2508
Mva1269I	GAATGC	4 cut(s)	499, 751, 763, 1603
MvaI	CCWGG	2 cut(s)	599, 987
MvnI	CGCG	1 cut(s)	1181
NaeI	GCCGGC	1 cut(s)	921
NciI	CCSGG	1 cut(s)	170
NcoI	CCATGG	1 cut(s)	2149
NdeI	CATATG	1 cut(s)	2731
NdeII	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
NgoMIV	GCCGGC	1 cut(s)	919
NlaIV	GGNNCC	7 cut(s)	76, 174, 188, 1630, 2054, 2316, 2456
NmeAIII	GCCGAG	2 cut(s)	1005, 2854
NmuCI	GTSAC	8 cut(s)	217, 236, 870, 1783, 1958, 2341, 2446, 2564
NsiI	ATGCAT	1 cut(s)	499
NspI	RCATGY	1 cut(s)	2440
NspV	TTCGAA	1 cut(s)	755
OliI	CACNNNNGTG	1 cut(s)	2571
PaeI	GCATGC	1 cut(s)	2440
PaeR7I	CTCGAG	1 cut(s)	290
PceI	AGGCCT	1 cut(s)	2596
PciSI	GCTCTTC	1 cut(s)	1983
PcsI	WCGNNNNNNNCGW	1 cut(s)	162
PctI	GAATGC	4 cut(s)	499, 751, 763, 1603
PdiI	GCCGGC	1 cut(s)	921
PdmI	GAANNNNTTC	4 cut(s)	1165, 1444, 2010, 2746
PfeI	GAWTC	4 cut(s)	149, 225, 1237, 2753
PflMI	CCANNNNNTGG	1 cut(s)	2155
PkrI	GCNGC	5 cut(s)	109, 200, 1216, 1260, 2706
PleI	GAGTC	7 cut(s)	588, 721, 990, 1493, 1726, 2474, 2839
PpsI	GAGTC	7 cut(s)	588, 721, 990, 1493, 1726, 2474, 2839
PpuMI	RGGWCCY	1 cut(s)	326
PshBI	ATTAAT	1 cut(s)	1529
Psp5II	RGGWCCY	1 cut(s)	326
Psp6I	CCWGG	2 cut(s)	597, 985
PspEI	GGTNACC	4 cut(s)	217, 236, 1040, 1349
PspFI	CCCAGC	5 cut(s)	20, 70, 1312, 1892, 2777
PspGI	CCWGG	2 cut(s)	597, 985
PspN4I	GGNNCC	7 cut(s)	76, 174, 188, 1630, 2054, 2316, 2456
PspPI	GGNCC	9 cut(s)	162, 172, 186, 326, 770, 839, 911, 2158, 2605
PspPPI	RGGWCCY	1 cut(s)	326
PspXI	VCTCGAGB	1 cut(s)	290
PstI	CTGCAG	2 cut(s)	112, 2848
PstNI	CAGNNNCTG	2 cut(s)	661, 2333
PsuI	RGATCY	1 cut(s)	1628
RsaI	GTAC	3 cut(s)	256, 1378, 2250
RsaNI	GTAC	3 cut(s)	255, 1377, 2249
RseI	CAYNNNNRTG	3 cut(s)	756, 2092, 2571
SapI	GCTCTTC	1 cut(s)	1983
SatI	GCNGC	5 cut(s)	108, 199, 1215, 1259, 2705
Sau3AI	GATC	9 cut(s)	372, 997, 1078, 1628, 1650, 2391, 2500, 2512, 2656
Sau96I	GGNCC	9 cut(s)	162, 172, 186, 326, 770, 839, 911, 2158, 2605
ScaI	AGTACT	1 cut(s)	1378
SchI	GAGTC	7 cut(s)	589, 721, 991, 1494, 1727, 2474, 2840
ScrFI	CCNGG	3 cut(s)	170, 599, 987
SduI	GDGCHC	4 cut(s)	1396, 1595, 1687, 1776
SfaNI	GCATC	6 cut(s)	94, 105, 506, 559, 1975, 2811
SfcI	CTRYAG	2 cut(s)	108, 2844
SfiI	GGCCNNNNNGGCC	1 cut(s)	527
Sfr274I	CTCGAG	1 cut(s)	290
SfuI	TTCGAA	1 cut(s)	755
SinI	GGWCC	7 cut(s)	162, 172, 186, 326, 770, 839, 911
SlaI	CTCGAG	1 cut(s)	290
SmiMI	CAYNNNNRTG	3 cut(s)	756, 2092, 2571
SmlI	CTYRAG	6 cut(s)	290, 488, 811, 866, 2034, 2714
SmoI	CTYRAG	6 cut(s)	290, 488, 811, 866, 2034, 2714
SphI	GCATGC	1 cut(s)	2440
SseBI	AGGCCT	1 cut(s)	2596
SsiI	CCGC	5 cut(s)	805, 1244, 1618, 2488, 2705
SspI	AATATT	1 cut(s)	2200
SspMI	CTAG	5 cut(s)	267, 875, 1449, 1668, 1710
StuI	AGGCCT	1 cut(s)	2596
StyD4I	CCNGG	3 cut(s)	168, 597, 985
StyI	CCWWGG	1 cut(s)	2149
TaaI	ACNGT	8 cut(s)	1129, 1273, 1398, 1445, 1539, 1657, 1837, 2548
TaiI	ACGT	1 cut(s)	1785
TaqI	TCGA	4 cut(s)	291, 615, 755, 1022
TatI	WGTACW	1 cut(s)	1376
TauI	GCSGC	1 cut(s)	2707
TfiI	GAWTC	4 cut(s)	149, 225, 1237, 2753
TseFI	GTSAC	8 cut(s)	217, 236, 870, 1783, 1958, 2341, 2446, 2564
TseI	GCWGC	4 cut(s)	107, 198, 1214, 1258
Tsp45I	GTSAC	8 cut(s)	217, 236, 870, 1783, 1958, 2341, 2446, 2564
TspDTI	ATGAA	9 cut(s)	17, 48, 1601, 1661, 1740, 2019, 2458, 2748, 2754
Van91I	CCANNNNNTGG	1 cut(s)	2155
Vha464I	CTTAAG	2 cut(s)	866, 2714
VneI	GTGCAC	2 cut(s)	1392, 1683
VpaK11BI	GGWCC	7 cut(s)	162, 172, 186, 326, 770, 839, 911
VspI	ATTAAT	1 cut(s)	1529
XagI	CCTNNNNNAGG	1 cut(s)	1359
XapI	RAATTY	5 cut(s)	318, 474, 651, 1405, 1922
XbaI	TCTAGA	1 cut(s)	1448
XceI	RCATGY	1 cut(s)	2440
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1089
XhoI	CTCGAG	1 cut(s)	290
XmnI	GAANNNNTTC	4 cut(s)	1165, 1444, 2010, 2746
XspI	CTAG	5 cut(s)	267, 875, 1449, 1668, 1710
ZrmI	AGTACT	1 cut(s)	1378
Zsp2I	ATGCAT	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.