Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0012762

Physical Location & Seq

Genomic Coordinates Reverse (-)

11925133 .. 11929239

Gene Structure 4107 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022769

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGGAGAAGAAGAAACCCCAACATGTTGGGTACTACTACAAGCTCTTGGCTTTTCTGGTTGCTGGGTTTTCTTCCCTGTTGCTCTGTGCCAACTCTCAACCAAGCGCAAGCAACGATGCTGCAGTAATGCTAGCCCTCAAGAAGAGCCTCAACCCGTCCAGTTCATTCGGATGGTCCGACCCGGACCCGTGCAAGTGGAGCTATGTAGGCTGCTCAGGTGACAATAGGGTGACCCGGATCCAGATTGGTCACCAAAACCTTCAAGGTACGCTCCCTACAAGCCTCCAAAACCTCACTCAGCTACAGCGATTAGAGGTTCAGTGGAACAAGATTTCGGGTCCTCTTCCAAGCCTAAGTGGGTTGGGCTCGTTACAAGTGTTGTTACTTGGTAACAACTTGTTTACTTCAATTCCTAGTGATTTCTTTACTGGCATGACCTCACTTCAGTCTGTTGAGATTGACAACAACCCATTTACGGCCTGGGAAATCCCCCAAACCCTCCAGAACGCATCATCACTTCAAAATTTCTCAGCCAATTCCGCCAACATTACCGGAAAAATACCGGATTTTTTCAACGCCGGTGCGTTTCCCGGGTTGGTTAACTTGCACTTGGCTTTCAATTACCTGGAGGGCCAATTGCCGGAGAGCTTTTCGGGGTCAGAAATGCAGTCTCTTTGGCTTAATGGGCAACAGAGTGTAGGTAAGCTTGTTGGCAGCATTGATGTTTTACGCAACATGACTACGCTGACTGAGGTTTGGTTGCATTCAAATGGGTTTTCGGGTCCATTGCCCGACTTTTCGGGGTTGACAGATTTGAGGAGCTTAAGTTTAAGAGATAATTTGTTCACCGGACCTGTTCCGGTGTCATTGTTGAACCTCAAGTCATTAGAGTCTGTGAATTTGACAAACAACTTGCTCCAAGGACCAACGCCAGATTTTCCCAAGGGGGTTTCAGTAGATATGACTAAGGATTCAAATAACTTTTGCCTGCCAAGTCCTGGCCCATGCGATCCTCGAGTGGATACTTTGCTTTTGATTGTTAGCTCAATGTCTTATCCTCAAAAGTTTGCAGAGAATTGGAAGGGGAATGATCCCTGTGCAGATTGGATCGGAGTCACTTGTAGAAATGGCAACATTACGGTCATCAATTTTCAGAAAATGGGTCTCACCGGAACCATTTCTCCGGCTTTTGCCTCACTTACGTCGCTCCAAAGACTGGTTCTTGCTGATAACAATTTGACAGGATCTATTCCAGAGGAGCTAGCCACTCTGCCTGCACTTACTCAATTAGATGTGTCAAACAATCTGCTTTATGGCAAAATCCCAGCTTTTACCAAGGCTAATGTGATTGTAAACACTGATGGCAACCCTGATATAGGGAAGGAAAAGAATACCAATGGGCCGTCTCAGAATTCAACTAACCCTTCTACAAGTATTTCTGGAAATGGTAACAGTTCTGGACCTCATGGGAAGAAATCTTCAAATTTAGTAGGAGTAATTGTGTTCTCTGTTATCGGTGCTGTTTTTGTGATGTTCTTAATTGCACTGTTGGTTATCTGTTTATATAGAACAAAACAAAAGCGGTTGACCAGAGTTCAGAGCCCAAATGCAATGGTGATCCATCCTCGCCATTCTGGATCTGATAATGAGAGCATGAAGATAACAGTTGCAGGCTCTAGCGTTAGCGTTGGTGCAATTAGTGAAGCTCACACTCTTCCTAGTAGTGAGCCAAGCGACATCCAAATGGTTGAAGCAGGAAACATGGTGATATCTATTCAAGTGCTACGGAGTGTGACCAACAATTTCAGTGAAGAAAATATATTGGGAAGAGGAGGCTTTGGGACAGTGTATAAAGGTGAACTGCATGACGGAACAAAGATTGCAGTTAAGAGAATGGAGGCTGGGGTAATAGCCGGAAAGGGAGACCTAAAGCCTTCAAACATTCTTCTTGGAGATGATATGAGGGCTAAGGTTGCAGATTTTGGTCTTGTGCGGCTTGCTCCAGAGGGTAAAGGTTCAATTGAAACAAGAATTGCAGGGACTTTCGGATACTTGGCACCAGAATATGCAGTGACTGGTCGTGTGACAACTAAAGTAGATGTATTCAGCTTCGGGGTGATTTTGATGGAGCTAATCACAGGGAGAAAAGCACTTGACGAGAGCCAGCCTGAGGAGAGCATGCACCTTGTCACATGGTTTCGGAGGATTTTTATTAATAAGGACTCGTTCCGCAAGGCCATTGATCCAACAATTGATCTTGACGAGGGAACACTTGCTAGTGTAAGCACTGTAGCAGAGCTAGCAGGTCATTGCTCCGCAAGAGAGCCCTATCAAAGGCCTGACATGAGCCATGCTGTTAATGTTCTATCTTCTCTGGTGGAGCTTTGGAAACCAAGTGATCAAAGCTCTGAAGACATATATGGTATCGATCTTGAAATGTCATTGCCACAAGCACTTAAGAAATGGCAGGCCTATGAAGGTAGGAGCAATATGGAATCCTCTTCGTCATTGTTTCCTCCAAGCTTGGACAATACCCAGACTAGCATCCCCACTCGCCCCTATGGCTTTGCCGAGTCTTTCACATCTGCAGATGGGAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

867

Amino Acids

93.49

Weight (kDa)

5.76

Isoelectric Point (pI)

36.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	37 - 72	2.7e-07	Leucine rich repeat N-terminal domain
LRRNT_2	PF08263	340 - 374	1.9e-06	Leucine rich repeat N-terminal domain
LRR_8	PF13855	396 - 437	2.4e-07	Leucine rich repeat
LRR_4	PF12799	401 - 441	1.6e-06	Leucine Rich repeats (2 copies)
Pkinase	PF00069	642 - 787	5.2e-19	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	642 - 732	8.2e-14	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66150
fragaria_vesca	FvH4_1g29920
malus_domestica	MD09G1266700.v1.1 MD17G1262700.v1.1
prunus_persica	Prupe.3G164300_v2.0.a1
pyrus_communis	pycom09g17970 pycom17g26270
rosa_chinensis	RchiOBHm_Chr3g0492431
rosa_laevigata	RLG00000022769
rosa_multiflora	Rmu_sc0003156.1_g000009 Rmu_sc0027181.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392320
rosa_rugosa	Rorug03G0253800
rosa_samantha	Rh3AG304000 Rh3BG339900 Rh3CG337100 Rh3DG339100
rosa_wichuraiana	Rw3G026800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2294
AccB1I	GGYRCC	1 cut(s)	2056
AccB7I	CCANNNNNTGG	2 cut(s)	998, 1216
AciI	CCGC	5 cut(s)	540, 1582, 1993, 2230, 2316
AclWI	GGATC	9 cut(s)	232, 245, 1004, 1085, 1115, 1252, 1612, 1645, 2237
AcsI	RAATTY	4 cut(s)	523, 898, 1411, 1483
AcuI	CTGAAG	2 cut(s)	428, 2430
AfaI	GTAC	2 cut(s)	32, 268
AfiI	CCNNNNNNNGG	7 cut(s)	475, 640, 998, 1216, 1376, 2170, 2334
AflII	CTTAAG	2 cut(s)	823, 2456
AflIII	ACRYGT	1 cut(s)	22
AjnI	CCWGG	3 cut(s)	479, 624, 997
AjuI	GAANNNNNNNTTGG	2 cut(s)	1203, 1235
AloI	GAACNNNNNNTCC	2 cut(s)	1165, 1197
Alw26I	GTCTC	4 cut(s)	675, 1169, 1410, 1917
AlwI	GGATC	9 cut(s)	232, 245, 1004, 1085, 1115, 1252, 1612, 1645, 2237
AlwNI	CAGNNNCTG	1 cut(s)	2075
Ama87I	CYCGRG	2 cut(s)	590, 1014
AoxI	GGCC	7 cut(s)	477, 631, 1000, 1400, 2235, 2336, 2469
ApeKI	GCWGC	3 cut(s)	119, 210, 714
ApoI	RAATTY	4 cut(s)	523, 898, 1411, 1483
AseI	ATTAAT	1 cut(s)	2214
Asp700I	GAANNNNTTC	1 cut(s)	1177
AspLEI	GCGC	1 cut(s)	107
AsuC2I	CCSGG	4 cut(s)	182, 235, 591, 592
AsuHPI	GGTGA	9 cut(s)	230, 241, 242, 838, 1159, 1627, 1777, 1868, 2128
AsuNHI	GCTAGC	3 cut(s)	130, 1261, 2299
AvaI	CYCGRG	2 cut(s)	590, 1014
AvaII	GGWCC	7 cut(s)	174, 184, 338, 782, 851, 923, 1460
AxyI	CCTNAGG	1 cut(s)	2169
BamHI	GGATCC	1 cut(s)	237
BanI	GGYRCC	1 cut(s)	2056
BanII	GRGCYC	3 cut(s)	368, 1604, 2328
BbsI	GAAGAC	1 cut(s)	2418
BbvI	GCAGC	3 cut(s)	106, 197, 726
BccI	CCATC	5 cut(s)	165, 1355, 1629, 2119, 2585
BceAI	ACGGC	2 cut(s)	492, 1387
BciT130I	CCWGG	3 cut(s)	481, 626, 999
BciVI	GTATCC	2 cut(s)	1015, 2042
BclI	TGATCA	1 cut(s)	2398
BcnI	CCSGG	4 cut(s)	182, 235, 591, 592
BcoDI	GTCTC	4 cut(s)	675, 1169, 1410, 1917
BfaI	CTAG	8 cut(s)	131, 414, 1262, 1677, 1719, 2277, 2300, 2541
BfmI	CTRYAG	4 cut(s)	120, 302, 2289, 2586
BfrI	CTTAAG	2 cut(s)	823, 2456
BfuAI	ACCTGC	1 cut(s)	2294
BfuI	GTATCC	2 cut(s)	1015, 2042
BglI	GCCNNNNNGGC	1 cut(s)	2562
BisI	GCNGC	4 cut(s)	120, 211, 715, 1994
BlsI	GCNGC	4 cut(s)	121, 212, 716, 1995
Bme1390I	CCNGG	7 cut(s)	182, 235, 481, 591, 592, 626, 999
Bme18I	GGWCC	7 cut(s)	174, 184, 338, 782, 851, 923, 1460
BmeT110I	CYCGRG	2 cut(s)	590, 1014
BmiI	GGNNCC	6 cut(s)	186, 239, 339, 783, 1174, 2058
BmrFI	CCNGG	7 cut(s)	182, 235, 481, 591, 592, 626, 999
BmsI	GCATC	3 cut(s)	106, 518, 2553
BmtI	GCTAGC	3 cut(s)	134, 1265, 2303
BpiI	GAAGAC	1 cut(s)	2418
BpmI	CTGGAG	3 cut(s)	485, 647, 1986
Bpu10I	CCTNAGC	2 cut(s)	214, 1968
BpuEI	CTTGAG	2 cut(s)	122, 863
BpuMI	CCSGG	4 cut(s)	182, 235, 591, 592
Bsa29I	ATCGAT	1 cut(s)	2427
BsaI	GGTCTC	2 cut(s)	1169, 1917
BsaJI	CCNNGG	5 cut(s)	480, 590, 919, 942, 1335
BsaWI	WCCGGW	5 cut(s)	551, 562, 848, 859, 1169
BsaXI	ACNNNNNCTCC	6 cut(s)	811, 841, 1165, 1195, 1485, 1515
Bsc4I	CCNNNNNNNGG	7 cut(s)	475, 640, 998, 1216, 1376, 2170, 2334
Bse118I	RCCGGY	1 cut(s)	578
Bse1I	ACTGG	4 cut(s)	159, 433, 1221, 2080
Bse21I	CCTNAGG	1 cut(s)	2169
Bse3DI	GCAATG	4 cut(s)	785, 1617, 2308, 2441
BseBI	CCWGG	3 cut(s)	481, 626, 999
BseCI	ATCGAT	1 cut(s)	2427
BseDI	CCNNGG	5 cut(s)	480, 590, 919, 942, 1335
BseGI	GGATG	4 cut(s)	176, 1621, 1737, 2544
BseLI	CCNNNNNNNGG	7 cut(s)	475, 640, 998, 1216, 1376, 2170, 2334
BseMI	GCAATG	4 cut(s)	785, 1617, 2308, 2441
BseMII	CTCAG	6 cut(s)	228, 311, 543, 741, 1421, 2160
BseNI	ACTGG	4 cut(s)	159, 433, 1221, 2080
BseRI	GAGGAG	4 cut(s)	832, 1271, 1845, 2186
BseXI	GCAGC	3 cut(s)	106, 197, 726
BseYI	CCCAGC	3 cut(s)	62, 1324, 1901
BsgI	GTGCAG	2 cut(s)	1119, 1260
BshFI	GGCC	7 cut(s)	479, 633, 1002, 1402, 2237, 2338, 2471
BshNI	GGYRCC	1 cut(s)	2056
BshVI	ATCGAT	1 cut(s)	2427
BsiHKCI	CYCGRG	2 cut(s)	590, 1014
BslFI	GGGAC	2 cut(s)	1855, 2053
BslI	CCNNNNNNNGG	7 cut(s)	475, 640, 998, 1216, 1376, 2170, 2334
BsmAI	GTCTC	4 cut(s)	675, 1169, 1410, 1917
BsmBI	CGTCTC	1 cut(s)	1410
BsmFI	GGGAC	2 cut(s)	1855, 2053
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	7 cut(s)	479, 633, 1002, 1402, 2237, 2338, 2471
Bso31I	GGTCTC	2 cut(s)	1169, 1917
BsoBI	CYCGRG	2 cut(s)	590, 1014
Bsp1286I	GDGCHC	3 cut(s)	368, 1604, 2328
BspACI	CCGC	5 cut(s)	540, 1582, 1993, 2230, 2316
BspANI	GGCC	7 cut(s)	479, 633, 1002, 1402, 2237, 2338, 2471
BspCNI	CTCAG	6 cut(s)	227, 310, 542, 742, 1420, 2161
BspDI	ATCGAT	1 cut(s)	2427
BspLI	GGNNCC	6 cut(s)	186, 239, 339, 783, 1174, 2058
BspMAI	CTGCAG	2 cut(s)	124, 2590
BspMI	ACCTGC	1 cut(s)	2294
BspOI	GCTAGC	3 cut(s)	134, 1265, 2303
BspPI	GGATC	9 cut(s)	232, 245, 1004, 1085, 1115, 1252, 1612, 1645, 2237
BspQI	GCTCTTC	1 cut(s)	137
BspT107I	GGYRCC	1 cut(s)	2056
BspTI	CTTAAG	2 cut(s)	823, 2456
BspTNI	GGTCTC	2 cut(s)	1169, 1917
BsrDI	GCAATG	4 cut(s)	785, 1617, 2308, 2441
BsrFI	RCCGGY	1 cut(s)	578
BsrI	ACTGG	4 cut(s)	159, 433, 1221, 2080
BssAI	RCCGGY	1 cut(s)	578
BssECI	CCNNGG	5 cut(s)	480, 590, 919, 942, 1335
BssT1I	CCWWGG	3 cut(s)	919, 942, 1335
Bst2UI	CCWGG	3 cut(s)	481, 626, 999
Bst4CI	ACNGT	6 cut(s)	1141, 1454, 1548, 1666, 1846, 2290
Bst6I	CTCTTC	5 cut(s)	137, 348, 1719, 1822, 2506
BstAFI	CTTAAG	2 cut(s)	823, 2456
BstDEI	CTNAG	9 cut(s)	214, 297, 353, 529, 750, 966, 1407, 1968, 2169
BstEII	GGTNACC	2 cut(s)	229, 248
BstENI	CCTNNNNNAGG	2 cut(s)	1374, 2332
BstF5I	GGATG	4 cut(s)	176, 1621, 1737, 2544
BstHHI	GCGC	1 cut(s)	107
BstMAI	GTCTC	4 cut(s)	675, 1169, 1410, 1917
BstMWI	GCNNNNNNNGC	7 cut(s)	198, 207, 539, 685, 1973, 2300, 2562
BstNI	CCWGG	3 cut(s)	481, 626, 999
BstNSI	RCATGY	2 cut(s)	26, 2182
BstPI	GGTNACC	2 cut(s)	229, 248
BstSCI	CCNGG	7 cut(s)	180, 233, 479, 589, 590, 624, 997
BstSFI	CTRYAG	4 cut(s)	120, 302, 2289, 2586
BstV1I	GCAGC	3 cut(s)	106, 197, 726
BstV2I	GAAGAC	1 cut(s)	2418
BstX2I	RGATCY	3 cut(s)	237, 1244, 1637
BstXI	CCANNNNNNTGG	1 cut(s)	26
BstYI	RGATCY	3 cut(s)	237, 1244, 1637
Bsu15I	ATCGAT	1 cut(s)	2427
Bsu36I	CCTNAGG	1 cut(s)	2169
BsuI	GTATCC	2 cut(s)	1015, 2042
BsuRI	GGCC	7 cut(s)	479, 633, 1002, 1402, 2237, 2338, 2471
BsuTUI	ATCGAT	1 cut(s)	2427
BtsCI	GGATG	4 cut(s)	176, 1621, 1737, 2544
BtsI	GCAGTG	1 cut(s)	2076
BtsIMutI	CAGTG	7 cut(s)	326, 1356, 1544, 1813, 1851, 2076, 2286
BveI	ACCTGC	1 cut(s)	2294
CaiI	CAGNNNCTG	1 cut(s)	2075
CfoI	GCGC	1 cut(s)	107
Cfr10I	RCCGGY	1 cut(s)	578
Cfr9I	CCCGGG	1 cut(s)	590
ClaI	ATCGAT	1 cut(s)	2427
Csp6I	GTAC	2 cut(s)	31, 267
CviQI	GTAC	2 cut(s)	31, 267
DdeI	CTNAG	9 cut(s)	214, 297, 353, 529, 750, 966, 1407, 1968, 2169
Eam1104I	CTCTTC	5 cut(s)	137, 348, 1719, 1822, 2506
EarI	CTCTTC	5 cut(s)	137, 348, 1719, 1822, 2506
EciI	GGCGGA	1 cut(s)	529
Eco130I	CCWWGG	3 cut(s)	919, 942, 1335
Eco147I	AGGCCT	2 cut(s)	2338, 2471
Eco24I	GRGCYC	3 cut(s)	368, 1604, 2328
Eco31I	GGTCTC	2 cut(s)	1169, 1917
Eco32I	GATATC	1 cut(s)	1770
Eco47I	GGWCC	7 cut(s)	174, 184, 338, 782, 851, 923, 1460
Eco57I	CTGAAG	2 cut(s)	428, 2430
Eco81I	CCTNAGG	1 cut(s)	2169
Eco88I	CYCGRG	2 cut(s)	590, 1014
Eco91I	GGTNACC	2 cut(s)	229, 248
EcoNI	CCTNNNNNAGG	2 cut(s)	1374, 2332
EcoO109I	RGGNCCY	1 cut(s)	338
EcoO65I	GGTNACC	2 cut(s)	229, 248
EcoRI	GAATTC	1 cut(s)	1411
EcoRII	CCWGG	3 cut(s)	479, 624, 997
EcoRV	GATATC	1 cut(s)	1770
EcoT14I	CCWWGG	3 cut(s)	919, 942, 1335
EcoT38I	GRGCYC	3 cut(s)	368, 1604, 2328
ErhI	CCWWGG	3 cut(s)	919, 942, 1335
Esp3I	CGTCTC	1 cut(s)	1410
FaqI	GGGAC	2 cut(s)	1855, 2053
FbaI	TGATCA	1 cut(s)	2398
Fnu4HI	GCNGC	4 cut(s)	120, 211, 715, 1994
FokI	GGATG	4 cut(s)	183, 1608, 1724, 2531
FriOI	GRGCYC	3 cut(s)	368, 1604, 2328
Fsp4HI	GCNGC	4 cut(s)	120, 211, 715, 1994
FspBI	CTAG	8 cut(s)	131, 414, 1262, 1677, 1719, 2277, 2300, 2541
GlaI	GCGC	1 cut(s)	106
GluI	GCNGC	4 cut(s)	120, 211, 715, 1994
GsaI	CCCAGC	3 cut(s)	66, 1328, 1905
GsuI	CTGGAG	3 cut(s)	485, 647, 1986
HaeIII	GGCC	7 cut(s)	479, 633, 1002, 1402, 2237, 2338, 2471
HhaI	GCGC	1 cut(s)	107
Hin6I	GCGC	1 cut(s)	105
HinP1I	GCGC	1 cut(s)	105
HincII	GTYRAC	3 cut(s)	601, 807, 1587
HindII	GTYRAC	3 cut(s)	601, 807, 1587
HindIII	AAGCTT	2 cut(s)	704, 2521
HinfI	GANTC	6 cut(s)	890, 971, 1113, 2222, 2495, 2573
HpaI	GTTAAC	1 cut(s)	601
HphI	GGTGA	9 cut(s)	230, 241, 242, 838, 1159, 1627, 1777, 1868, 2128
Hpy166II	GTNNAC	7 cut(s)	402, 601, 807, 846, 1354, 1587, 1859
Hpy8I	GTNNAC	7 cut(s)	402, 601, 807, 846, 1354, 1587, 1859
Hpy99I	CGWCG	1 cut(s)	1207
HpyAV	CCTTC	6 cut(s)	269, 1075, 1375, 1434, 1944, 2471
HpyCH4III	ACNGT	6 cut(s)	1141, 1454, 1548, 1666, 1846, 2290
HpyCH4IV	ACGT	1 cut(s)	1202
HpyF10VI	GCNNNNNNNGC	7 cut(s)	198, 207, 539, 685, 1973, 2300, 2562
HpyF3I	CTNAG	9 cut(s)	214, 297, 353, 529, 750, 966, 1407, 1968, 2169
HpySE526I	ACGT	1 cut(s)	1202
HspAI	GCGC	1 cut(s)	105
Ksp22I	TGATCA	1 cut(s)	2398
KspAI	GTTAAC	1 cut(s)	601
LguI	GCTCTTC	1 cut(s)	137
Lsp1109I	GCAGC	3 cut(s)	106, 197, 726
LweI	GCATC	3 cut(s)	106, 518, 2553
MaeI	CTAG	8 cut(s)	131, 414, 1262, 1677, 1719, 2277, 2300, 2541
MaeII	ACGT	1 cut(s)	1202
MfeI	CAATTG	3 cut(s)	635, 2019, 2250
MflI	RGATCY	3 cut(s)	237, 1244, 1637
MhlI	GDGCHC	3 cut(s)	368, 1604, 2328
MlyI	GAGTC	4 cut(s)	899, 1122, 2216, 2582
MmeI	TCCRAC	2 cut(s)	201, 2270
MroXI	GAANNNNTTC	1 cut(s)	1177
MseI	TTAA	9 cut(s)	600, 681, 824, 830, 1538, 1887, 2214, 2358, 2457
MslI	CAYNNNNRTG	3 cut(s)	169, 768, 1742
MspCI	CTTAAG	2 cut(s)	823, 2456
MspR9I	CCNGG	7 cut(s)	182, 235, 481, 591, 592, 626, 999
MunI	CAATTG	3 cut(s)	635, 2019, 2250
Mva1269I	GAATGC	1 cut(s)	763
MvaI	CCWGG	3 cut(s)	481, 626, 999
MwoI	GCNNNNNNNGC	7 cut(s)	198, 207, 539, 685, 1973, 2300, 2562
NciI	CCSGG	4 cut(s)	182, 235, 591, 592
NheI	GCTAGC	3 cut(s)	130, 1261, 2299
NlaIV	GGNNCC	6 cut(s)	186, 239, 339, 783, 1174, 2058
NmeAIII	GCCGAG	1 cut(s)	2596
NmuCI	GTSAC	8 cut(s)	218, 229, 248, 1114, 1792, 2071, 2083, 2188
NspI	RCATGY	2 cut(s)	26, 2182
PaeI	GCATGC	1 cut(s)	2182
PaeR7I	CTCGAG	1 cut(s)	1014
PceI	AGGCCT	2 cut(s)	2338, 2471
PciI	ACATGT	1 cut(s)	22
PciSI	GCTCTTC	1 cut(s)	137
PcsI	WCGNNNNNNNCGW	1 cut(s)	174
PctI	GAATGC	1 cut(s)	763
PdmI	GAANNNNTTC	1 cut(s)	1177
PfeI	GAWTC	2 cut(s)	971, 2495
PflMI	CCANNNNNTGG	2 cut(s)	998, 1216
PkrI	GCNGC	4 cut(s)	121, 212, 716, 1995
PleI	GAGTC	4 cut(s)	898, 1121, 2216, 2581
PpsI	GAGTC	4 cut(s)	898, 1121, 2216, 2581
PpuMI	RGGWCCY	1 cut(s)	338
PscI	ACATGT	1 cut(s)	22
PshBI	ATTAAT	1 cut(s)	2214
Psp5II	RGGWCCY	1 cut(s)	338
Psp6I	CCWGG	3 cut(s)	479, 624, 997
PspEI	GGTNACC	2 cut(s)	229, 248
PspFI	CCCAGC	3 cut(s)	62, 1324, 1901
PspGI	CCWGG	3 cut(s)	479, 624, 997
PspN4I	GGNNCC	6 cut(s)	186, 239, 339, 783, 1174, 2058
PspPPI	RGGWCCY	1 cut(s)	338
PspXI	VCTCGAGB	1 cut(s)	1014
PstI	CTGCAG	2 cut(s)	124, 2590
PstNI	CAGNNNCTG	1 cut(s)	2075
PsuI	RGATCY	3 cut(s)	237, 1244, 1637
RsaI	GTAC	2 cut(s)	32, 268
RsaNI	GTAC	2 cut(s)	31, 267
RseI	CAYNNNNRTG	3 cut(s)	169, 768, 1742
SapI	GCTCTTC	1 cut(s)	137
SaqAI	TTAA	9 cut(s)	600, 681, 824, 830, 1538, 1887, 2214, 2358, 2457
SatI	GCNGC	4 cut(s)	120, 211, 715, 1994
SchI	GAGTC	4 cut(s)	899, 1122, 2216, 2582
ScrFI	CCNGG	7 cut(s)	182, 235, 481, 591, 592, 626, 999
SduI	GDGCHC	3 cut(s)	368, 1604, 2328
SfaNI	GCATC	3 cut(s)	106, 518, 2553
SfcI	CTRYAG	4 cut(s)	120, 302, 2289, 2586
Sfr274I	CTCGAG	1 cut(s)	1014
SgrAI	CRCCGGYG	1 cut(s)	578
SinI	GGWCC	7 cut(s)	174, 184, 338, 782, 851, 923, 1460
SlaI	CTCGAG	1 cut(s)	1014
SmaI	CCCGGG	1 cut(s)	592
SmiMI	CAYNNNNRTG	3 cut(s)	169, 768, 1742
SmlI	CTYRAG	5 cut(s)	137, 823, 878, 1014, 2456
SmoI	CTYRAG	5 cut(s)	137, 823, 878, 1014, 2456
SphI	GCATGC	1 cut(s)	2182
SseBI	AGGCCT	2 cut(s)	2338, 2471
SsiI	CCGC	5 cut(s)	540, 1582, 1993, 2230, 2316
SspMI	CTAG	8 cut(s)	131, 414, 1262, 1677, 1719, 2277, 2300, 2541
StuI	AGGCCT	2 cut(s)	2338, 2471
StyD4I	CCNGG	7 cut(s)	180, 233, 479, 589, 590, 624, 997
StyI	CCWWGG	3 cut(s)	919, 942, 1335
TaaI	ACNGT	6 cut(s)	1141, 1454, 1548, 1666, 1846, 2290
TaiI	ACGT	1 cut(s)	1205
TaqI	TCGA	2 cut(s)	1015, 2427
TauI	GCSGC	1 cut(s)	1996
TfiI	GAWTC	2 cut(s)	971, 2495
Tru1I	TTAA	9 cut(s)	600, 681, 824, 830, 1538, 1887, 2214, 2358, 2457
Tru9I	TTAA	9 cut(s)	600, 681, 824, 830, 1538, 1887, 2214, 2358, 2457
TscAI	CASTG	7 cut(s)	326, 1363, 1551, 1813, 1851, 2076, 2293
TseFI	GTSAC	8 cut(s)	218, 229, 248, 1114, 1792, 2071, 2083, 2188
TseI	GCWGC	3 cut(s)	119, 210, 714
Tsp45I	GTSAC	8 cut(s)	218, 229, 248, 1114, 1792, 2071, 2083, 2188
TspDTI	ATGAA	3 cut(s)	153, 1670, 2490
TspGWI	ACGGA	2 cut(s)	1801, 1884
TspMI	CCCGGG	1 cut(s)	590
TspRI	CASTG	7 cut(s)	326, 1363, 1551, 1813, 1851, 2076, 2293
Van91I	CCANNNNNTGG	2 cut(s)	998, 1216
Vha464I	CTTAAG	2 cut(s)	823, 2456
VpaK11BI	GGWCC	7 cut(s)	174, 184, 338, 782, 851, 923, 1460
VspI	ATTAAT	1 cut(s)	2214
XagI	CCTNNNNNAGG	2 cut(s)	1374, 2332
XapI	RAATTY	4 cut(s)	523, 898, 1411, 1483
XceI	RCATGY	2 cut(s)	26, 2182
XcmI	CCANNNNNNNNNTGG	1 cut(s)	477
XhoI	CTCGAG	1 cut(s)	1014
XmaI	CCCGGG	1 cut(s)	590
XmnI	GAANNNNTTC	1 cut(s)	1177
XspI	CTAG	8 cut(s)	131, 414, 1262, 1677, 1719, 2277, 2300, 2541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.