MD17G1264300.v1.1
TCP Family

assists the folding of proteins upon ATP hydrolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010112
Physical Location & Seq
Forward (+)
32495903 .. 32502214
6312 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1264300.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGCAGCCCTACGGAATACAATCGATGCTGAAGGAGGGCCACAAGCACCTCTCCGGTTTGGACGAGGCGGTGGTGAAGAACATCGATGCCTGCAAGCAACTCTCCACCATCACCAGAACTTCTCTCGGTCCCAATGGTATGAATAAGATGGTAATCAATCACTTGGACAAGCTATTTGTGACAAATGATGCCGCCACCATTGTCAATGAACTGGAGGTTCAGCATCCTGCTGCCAAGATTTTGGTTTTGGCAGGCAGGGCTCAGCAGGAAGAAATCGGCGACGGCGCTAATCTCACCATTTCTTTTGCCGGGGAGCTCCTTCAAAACGCAGAGGAGCTTATCAGGATGGGTCTGCACCCGAGTGAGATCATTAGTGGATACAACAAAGCCATAAAAAAGACGCTTGAAGTCTTAGATGAACTGGTTGAGGAAGGTTCCGAGATTATGGACGTGCGCAACAAAGAACAAGTGGTTTCTAGAATGAGAGCTGCTGTTGCCAGCAAGCAATTTGGCCAAGAAGATATCTTGTCCTCACTTGTTGCCGATGCATGTATACAAGTATGCCCCAAGAACCCAGCAAACTTCAATGTGGATAATGTACGTGTTGCAAAGATTGCAGGAGGGGGTTTGCATAATTGTACAGTAGTTCGAGGTATGGTGTTGAAAACTGATGCTGTGGGGAGTATAAAGCGAATTGAGAAGGCCAAGGTTGCTGTGTTTGCGGGTGGAGTTGATACCTCTGCAACTGAGACGAAGGGAACTGTTCTAATTCATACTGCTGACCAGCTTGAAAATTATGCAAAAACTGAAGAAGCTAAAATTGAGGAGCTCATTAAAGCAGTTGCAGATTCTGGTGCCAAGGTAATTGTGAGTGGAGCAGCAGTTGGAGAGATGGCACTACATTTCTGTGAGCGGTACAAGCTCATGGTGCTGAAAATCAGTTCAAAATTTGAATTGAGACGATTTTGCCGTACAACTGGTGCTGTTGCCATGTTGAAGCTTAGCCAACCTAACCCAGATGATCTGGGATATGCTGACTCTGTCGCGGTTGAGGAAATTGCCGGTGTTAGGGTCACTGTTGTGAAGAATGAAGAAGGTGGGAATTCAGTAGCTACTGTGGTTCTACGGGCAAGTACTGATAGTATATTAGATGACCTTGAAAGAGCAGTTGATGATGGAGTGAACACATACAAGGCCATGTGCAAGGATAGCCGCATTGTTCCTGGAGCTGCTGCTACTGAAATTGAGTTAGCTAGAAGAGTGAAGGAATTCTCTTTTAAAGAAACAGGATTGGATCAGTATGCTATAGCAAAATTTGCCGAAAGTTTTGAGATGGTGCCTAAAACTCTGGCTGAGAATGCTGGGAAGAATGCAATGGAGATCATATCTTCTCTCTATGCTGAACATGCATCAGGAAATACGAAAGCGGGCATTGACTTAGAGGAAGGCCGTTGCAAAGATGTGTCGACTATTAATGTTTGGGACCTCCACATAACTAAGTTCTTTGCTCTCAAGTATGCTGTGGATGCTGCCTGCACTGTCCTAAGAGTAGATCAAATTATAATGGCAAAACCAGCAGGTGGCCCAAGTAAGAGAGATCAGCCTGCAGGGATGGATGAGGATTAA

Protein Analysis

542

Amino Acids

58.35

Weight (kDa)

5.45

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 34 - 525 1.6e-156 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1562
AarI CACCTGC 1 cut(s) 1568
Acc16I TGCGCA 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 1568
AccB1I GGYRCC 2 cut(s) 854, 1336
AccB7I CCANNNNNTGG 1 cut(s) 1580
AccBSI CCGCTC 1 cut(s) 913
AccI GTMKAC 2 cut(s) 553, 1466
AccII CGCG 1 cut(s) 1046
AciI CCGC 7 cut(s) 68, 192, 722, 913, 1046, 1213, 1427
AclWI GGATC 1 cut(s) 1302
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 4 cut(s) 947, 1102, 1268, 1313
AcuI CTGAAG 2 cut(s) 50, 828
AfaI GTAC 5 cut(s) 600, 640, 917, 973, 1135
AfiI CCNNNNNNNGG 2 cut(s) 1068, 1580
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 9 cut(s) 323, 407, 586, 664, 791, 945, 953, 997, 1160
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 1 cut(s) 1222
AleI CACNNNNGTG 2 cut(s) 360, 1079
Alw21I GWGCWC 2 cut(s) 318, 831
Alw26I GTCTC 2 cut(s) 743, 952
AlwI GGATC 1 cut(s) 1302
AlwNI CAGNNNCTG 1 cut(s) 851
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 6 cut(s) 37, 511, 702, 1194, 1447, 1582
ApeKI GCWGC 7 cut(s) 4, 230, 488, 878, 1229, 1232, 1529
ApoI RAATTY 4 cut(s) 947, 1102, 1268, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AseI ATTAAT 1 cut(s) 1473
Asp700I GAANNNNTTC 1 cut(s) 1268
AspLEI GCGC 2 cut(s) 287, 456
AspS9I GGNCC 4 cut(s) 37, 128, 1483, 1583
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 3 cut(s) 85, 103, 286
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 2 cut(s) 128, 1483
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 2 cut(s) 854, 1336
BanII GRGCYC 3 cut(s) 262, 318, 831
Bbv12I GWGCWC 2 cut(s) 318, 831
BbvI GCAGC 7 cut(s) 16, 217, 475, 890, 1216, 1219, 1516
BccI CCATC 7 cut(s) 116, 142, 340, 886, 1169, 1327, 1606
BceAI ACGGC 3 cut(s) 298, 954, 1434
BciT130I CCWGG 1 cut(s) 1224
BciVI GTATCC 1 cut(s) 371
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 743, 952
BfaI CTAG 2 cut(s) 477, 1254
BfmI CTRYAG 2 cut(s) 1305, 1605
BfoI RGCGCY 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 1568
BfuI GTATCC 1 cut(s) 371
BisI GCNGC 9 cut(s) 5, 192, 231, 489, 879, 1213, 1230, 1233, 1530
BlpI GCTNAGC 2 cut(s) 261, 1001
BlsI GCNGC 9 cut(s) 6, 193, 232, 490, 880, 1214, 1231, 1234, 1531
BmcAI AGTACT 1 cut(s) 1135
Bme1390I CCNGG 2 cut(s) 310, 1224
Bme18I GGWCC 2 cut(s) 128, 1483
BmeT110I CYCGRG 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 4 cut(s) 37, 128, 1483, 1583
BmiI GGNNCC 5 cut(s) 130, 436, 856, 1338, 1484
BmrFI CCNGG 2 cut(s) 310, 1224
BmsI GCATC 8 cut(s) 15, 76, 178, 232, 535, 661, 1418, 1516
BpmI CTGGAG 2 cut(s) 233, 1245
Bpu1102I GCTNAGC 2 cut(s) 261, 1001
BpuEI CTTGAG 1 cut(s) 1496
BpuMI CCSGG 1 cut(s) 310
Bsa29I ATCGAT 2 cut(s) 23, 84
BsaAI YACGTR 1 cut(s) 602
BsaBI GATNNNNATC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 309, 705, 858
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 1068, 1580
Bse118I RCCGGY 1 cut(s) 1061
Bse1I ACTGG 3 cut(s) 216, 426, 982
Bse3DI GCAATG 1 cut(s) 1380
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 1 cut(s) 1224
BseCI ATCGAT 2 cut(s) 23, 84
BseDI CCNNGG 3 cut(s) 309, 705, 858
BseGI GGATG 5 cut(s) 223, 351, 1531, 1617, 1621
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 2 cut(s) 1068, 1580
BseMI GCAATG 1 cut(s) 1380
BseMII CTCAG 3 cut(s) 275, 738, 1344
BseNI ACTGG 3 cut(s) 216, 426, 982
BseRI GAGGAG 2 cut(s) 347, 839
BseXI GCAGC 7 cut(s) 16, 217, 475, 890, 1216, 1219, 1516
BseYI CCCAGC 2 cut(s) 574, 1361
BsgI GTGCAG 2 cut(s) 338, 1519
Bsh1236I CGCG 1 cut(s) 1046
BshFI GGCC 6 cut(s) 39, 513, 704, 1196, 1449, 1584
BshNI GGYRCC 2 cut(s) 854, 1336
BshVI ATCGAT 2 cut(s) 23, 84
BsiHKAI GWGCWC 2 cut(s) 318, 831
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 3 cut(s) 54, 309, 1062
BslFI GGGAC 2 cut(s) 114, 1496
BslI CCNNNNNNNGG 2 cut(s) 1068, 1580
BsmAI GTCTC 2 cut(s) 743, 952
BsmBI CGTCTC 2 cut(s) 743, 952
BsmFI GGGAC 2 cut(s) 114, 1496
BsmI GAATGC 2 cut(s) 1363, 1375
BsnI GGCC 6 cut(s) 39, 513, 704, 1196, 1449, 1584
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 262, 318, 831
Bsp1407I TGTACA 1 cut(s) 638
Bsp143I GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
Bsp1720I GCTNAGC 2 cut(s) 261, 1001
BspACI CCGC 7 cut(s) 68, 192, 722, 913, 1046, 1213, 1427
BspANI GGCC 6 cut(s) 39, 513, 704, 1196, 1449, 1584
BspCNI CTCAG 3 cut(s) 274, 739, 1345
BspDI ATCGAT 2 cut(s) 23, 84
BspFNI CGCG 1 cut(s) 1046
BspLI GGNNCC 5 cut(s) 130, 436, 856, 1338, 1484
BspMAI CTGCAG 1 cut(s) 1609
BspMI ACCTGC 1 cut(s) 1568
BspPI GGATC 1 cut(s) 1302
BspT107I GGYRCC 2 cut(s) 854, 1336
BsrBI CCGCTC 1 cut(s) 913
BsrDI GCAATG 1 cut(s) 1380
BsrFI RCCGGY 1 cut(s) 1061
BsrGI TGTACA 1 cut(s) 638
BsrI ACTGG 3 cut(s) 216, 426, 982
BssAI RCCGGY 1 cut(s) 1061
BssECI CCNNGG 3 cut(s) 309, 705, 858
BssMI GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
BssNAI GTATAC 1 cut(s) 554
BssT1I CCWWGG 2 cut(s) 705, 858
Bst1107I GTATAC 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 1224
Bst4CI ACNGT 5 cut(s) 643, 763, 1078, 1117, 1540
Bst6I CTCTTC 1 cut(s) 1252
BstAPI GCANNNNNTGC 2 cut(s) 614, 1316
BstAUI TGTACA 1 cut(s) 638
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 8 cut(s) 91, 95, 253, 499, 503, 1429, 1534, 1605
BstDEI CTNAG 8 cut(s) 261, 412, 747, 1001, 1353, 1438, 1497, 1544
BstF5I GGATG 5 cut(s) 223, 351, 1531, 1617, 1621
BstFNI CGCG 1 cut(s) 1046
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 287, 456
BstKTI GATC 6 cut(s) 369, 1024, 1297, 1383, 1555, 1600
BstMAI GTCTC 2 cut(s) 743, 952
BstMBI GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
BstNI CCWGG 1 cut(s) 1224
BstNSI RCATGY 2 cut(s) 552, 1409
BstSCI CCNGG 2 cut(s) 308, 1222
BstSFI CTRYAG 2 cut(s) 1305, 1605
BstUI CGCG 1 cut(s) 1046
BstV1I GCAGC 7 cut(s) 16, 217, 475, 890, 1216, 1219, 1516
BstXI CCANNNNNNTGG 1 cut(s) 241
BstZ17I GTATAC 1 cut(s) 554
Bsu15I ATCGAT 2 cut(s) 23, 84
BsuI GTATCC 1 cut(s) 371
BsuRI GGCC 6 cut(s) 39, 513, 704, 1196, 1449, 1584
BsuTUI ATCGAT 2 cut(s) 23, 84
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 5 cut(s) 223, 351, 1531, 1617, 1621
BtsIMutI CAGTG 2 cut(s) 1074, 1536
BveI ACCTGC 1 cut(s) 1568
Cac8I GCNNGC 8 cut(s) 91, 95, 253, 499, 503, 1429, 1534, 1605
CaiI CAGNNNCTG 1 cut(s) 851
CfoI GCGC 2 cut(s) 287, 456
Cfr10I RCCGGY 1 cut(s) 1061
Cfr13I GGNCC 4 cut(s) 37, 128, 1483, 1583
ClaI ATCGAT 2 cut(s) 23, 84
CseI GACGC 1 cut(s) 409
Csp6I GTAC 5 cut(s) 599, 639, 916, 972, 1134
CviAII CATG 5 cut(s) 549, 925, 991, 1198, 1406
CviQI GTAC 5 cut(s) 599, 639, 916, 972, 1134
DdeI CTNAG 8 cut(s) 261, 412, 747, 1001, 1353, 1438, 1497, 1544
DpnI GATC 6 cut(s) 368, 1023, 1296, 1382, 1554, 1599
DpnII GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
DraI TTTAAA 1 cut(s) 1279
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 1252
EarI CTCTTC 1 cut(s) 1252
Ecl136II GAGCTC 2 cut(s) 316, 829
Eco130I CCWWGG 2 cut(s) 705, 858
Eco24I GRGCYC 3 cut(s) 262, 318, 831
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 2 cut(s) 128, 1483
Eco53kI GAGCTC 2 cut(s) 316, 829
Eco57I CTGAAG 2 cut(s) 50, 828
Eco88I CYCGRG 1 cut(s) 358
EcoICRI GAGCTC 2 cut(s) 316, 829
EcoO109I RGGNCCY 1 cut(s) 1483
EcoRI GAATTC 2 cut(s) 1102, 1268
EcoRII CCWGG 1 cut(s) 1222
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 705, 858
EcoT22I ATGCAT 2 cut(s) 550, 1411
EcoT38I GRGCYC 3 cut(s) 262, 318, 831
ErhI CCWWGG 2 cut(s) 705, 858
Esp3I CGTCTC 2 cut(s) 743, 952
FaeI CATG 5 cut(s) 552, 928, 994, 1201, 1409
FaqI GGGAC 2 cut(s) 114, 1496
FatI CATG 5 cut(s) 548, 924, 990, 1197, 1405
FauI CCCGC 2 cut(s) 715, 1420
FblI GTMKAC 2 cut(s) 553, 1466
Fnu4HI GCNGC 9 cut(s) 5, 192, 231, 489, 879, 1213, 1230, 1233, 1530
FokI GGATG 3 cut(s) 210, 358, 1538
FriOI GRGCYC 3 cut(s) 262, 318, 831
Fsp4HI GCNGC 9 cut(s) 5, 192, 231, 489, 879, 1213, 1230, 1233, 1530
FspBI CTAG 2 cut(s) 477, 1254
FspI TGCGCA 1 cut(s) 455
GlaI GCGC 2 cut(s) 286, 455
GluI GCNGC 9 cut(s) 5, 192, 231, 489, 879, 1213, 1230, 1233, 1530
GsaI CCCAGC 2 cut(s) 578, 1365
GsuI CTGGAG 2 cut(s) 233, 1245
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 6 cut(s) 39, 513, 704, 1196, 1449, 1584
HapII CCGG 3 cut(s) 54, 309, 1062
HgaI GACGC 1 cut(s) 409
HhaI GCGC 2 cut(s) 287, 456
Hin1II CATG 5 cut(s) 552, 928, 994, 1201, 1409
Hin6I GCGC 2 cut(s) 285, 454
HinP1I GCGC 2 cut(s) 285, 454
HincII GTYRAC 1 cut(s) 1467
HindII GTYRAC 1 cut(s) 1467
HindIII AAGCTT 1 cut(s) 998
HinfI GANTC 2 cut(s) 848, 1037
HpaII CCGG 3 cut(s) 54, 309, 1062
HphI GGTGA 3 cut(s) 85, 103, 286
Hpy166II GTNNAC 3 cut(s) 554, 1183, 1467
Hpy188I TCNGA 1 cut(s) 439
Hpy188III TCNNGA 3 cut(s) 343, 477, 1413
Hpy8I GTNNAC 3 cut(s) 554, 1183, 1467
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 8 cut(s) 25, 329, 425, 694, 748, 1088, 1258, 1439
HpyCH4III ACNGT 5 cut(s) 643, 763, 1078, 1117, 1540
HpyCH4IV ACGT 2 cut(s) 450, 601
HpyF3I CTNAG 8 cut(s) 261, 412, 747, 1001, 1353, 1438, 1497, 1544
HpySE526I ACGT 2 cut(s) 450, 601
Hsp92II CATG 5 cut(s) 552, 928, 994, 1201, 1409
HspAI GCGC 2 cut(s) 285, 454
Kzo9I GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
LmnI GCTCC 6 cut(s) 313, 321, 334, 826, 875, 1226
Lsp1109I GCAGC 7 cut(s) 16, 217, 475, 890, 1216, 1219, 1516
LweI GCATC 8 cut(s) 15, 76, 178, 232, 535, 661, 1418, 1516
MaeI CTAG 2 cut(s) 477, 1254
MaeII ACGT 2 cut(s) 450, 601
MaeIII GTNAC 2 cut(s) 178, 1072
MalI GATC 6 cut(s) 368, 1023, 1296, 1382, 1554, 1599
MbiI CCGCTC 1 cut(s) 913
MboI GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
MboII GAAGA 9 cut(s) 88, 281, 530, 821, 1096, 1103, 1269, 1378, 1380
MhlI GDGCHC 3 cut(s) 262, 318, 831
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 1 cut(s) 865
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 2 cut(s) 550, 1411
MroXI GAANNNNTTC 1 cut(s) 1268
MscI TGGCCA 1 cut(s) 513
MseI TTAA 4 cut(s) 834, 1278, 1473, 1624
MslI CAYNNNNRTG 3 cut(s) 360, 906, 1079
Msp20I TGGCCA 1 cut(s) 513
MspI CCGG 3 cut(s) 54, 309, 1062
MspR9I CCNGG 2 cut(s) 310, 1224
Mva1269I GAATGC 2 cut(s) 1363, 1375
MvaI CCWGG 1 cut(s) 1224
MvnI CGCG 1 cut(s) 1046
NciI CCSGG 1 cut(s) 310
NdeII GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
NlaIII CATG 5 cut(s) 552, 928, 994, 1201, 1409
NlaIV GGNNCC 5 cut(s) 130, 436, 856, 1338, 1484
NmuCI GTSAC 2 cut(s) 178, 1072
NsbI TGCGCA 1 cut(s) 455
NsiI ATGCAT 2 cut(s) 550, 1411
NspI RCATGY 2 cut(s) 552, 1409
OliI CACNNNNGTG 2 cut(s) 360, 1079
PaqCI CACCTGC 1 cut(s) 1568
PcsI WCGNNNNNNNCGW 1 cut(s) 967
PctI GAATGC 2 cut(s) 1363, 1375
PdmI GAANNNNTTC 1 cut(s) 1268
PfeI GAWTC 1 cut(s) 848
PflFI GACNNNGTC 1 cut(s) 1040
PflMI CCANNNNNTGG 1 cut(s) 1580
PfoI TCCNGGA 1 cut(s) 1222
PkrI GCNGC 9 cut(s) 6, 193, 232, 490, 880, 1214, 1231, 1234, 1531
PleI GAGTC 1 cut(s) 1031
PpsI GAGTC 1 cut(s) 1031
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 1483
PshBI ATTAAT 1 cut(s) 1473
PsiI TTATAA 1 cut(s) 1562
Psp124BI GAGCTC 2 cut(s) 318, 831
Psp5II RGGWCCY 1 cut(s) 1483
Psp6I CCWGG 1 cut(s) 1222
PspFI CCCAGC 2 cut(s) 574, 1361
PspGI CCWGG 1 cut(s) 1222
PspN4I GGNNCC 5 cut(s) 130, 436, 856, 1338, 1484
PspPI GGNCC 4 cut(s) 37, 128, 1483, 1583
PspPPI RGGWCCY 1 cut(s) 1483
PstI CTGCAG 1 cut(s) 1609
PstNI CAGNNNCTG 1 cut(s) 851
PsyI GACNNNGTC 1 cut(s) 1040
RsaI GTAC 5 cut(s) 600, 640, 917, 973, 1135
RsaNI GTAC 5 cut(s) 599, 639, 916, 972, 1134
RseI CAYNNNNRTG 3 cut(s) 360, 906, 1079
SacI GAGCTC 2 cut(s) 318, 831
SalI GTCGAC 1 cut(s) 1465
SaqAI TTAA 4 cut(s) 834, 1278, 1473, 1624
SatI GCNGC 9 cut(s) 5, 192, 231, 489, 879, 1213, 1230, 1233, 1530
Sau3AI GATC 6 cut(s) 366, 1021, 1294, 1380, 1552, 1597
Sau96I GGNCC 4 cut(s) 37, 128, 1483, 1583
SbfI CCTGCAGG 1 cut(s) 1609
ScaI AGTACT 1 cut(s) 1135
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 2 cut(s) 310, 1224
SdaI CCTGCAGG 1 cut(s) 1609
SduI GDGCHC 3 cut(s) 262, 318, 831
SfaNI GCATC 8 cut(s) 15, 76, 178, 232, 535, 661, 1418, 1516
SfcI CTRYAG 2 cut(s) 1305, 1605
SinI GGWCC 2 cut(s) 128, 1483
SmiMI CAYNNNNRTG 3 cut(s) 360, 906, 1079
SmlI CTYRAG 1 cut(s) 1511
SmoI CTYRAG 1 cut(s) 1511
Sse8387I CCTGCAGG 1 cut(s) 1609
SsiI CCGC 7 cut(s) 68, 192, 722, 913, 1046, 1213, 1427
SspMI CTAG 2 cut(s) 477, 1254
SstI GAGCTC 2 cut(s) 318, 831
StyD4I CCNGG 2 cut(s) 308, 1222
StyI CCWWGG 2 cut(s) 705, 858
TaaI ACNGT 5 cut(s) 643, 763, 1078, 1117, 1540
TaiI ACGT 2 cut(s) 453, 604
TaqI TCGA 4 cut(s) 23, 84, 649, 1466
TaqII GACCGA 1 cut(s) 116
TatI WGTACW 2 cut(s) 638, 1133
TauI GCSGC 2 cut(s) 194, 1215
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 4 cut(s) 834, 1278, 1473, 1624
Tru9I TTAA 4 cut(s) 834, 1278, 1473, 1624
TscAI CASTG 2 cut(s) 1081, 1543
TseFI GTSAC 2 cut(s) 178, 1072
TseI GCWGC 7 cut(s) 4, 230, 488, 878, 1229, 1232, 1529
Tsp45I GTSAC 2 cut(s) 178, 1072
TspDTI ATGAA 5 cut(s) 155, 222, 432, 761, 1104
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 2 cut(s) 1081, 1543
Tth111I GACNNNGTC 1 cut(s) 1040
Van91I CCANNNNNTGG 1 cut(s) 1580
VpaK11BI GGWCC 2 cut(s) 128, 1483
VspI ATTAAT 1 cut(s) 1473
XapI RAATTY 4 cut(s) 947, 1102, 1268, 1313
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 2 cut(s) 552, 1409
XmiI GTMKAC 2 cut(s) 553, 1466
XmnI GAANNNNTTC 1 cut(s) 1268
XspI CTAG 2 cut(s) 477, 1254
ZrmI AGTACT 1 cut(s) 1135
Zsp2I ATGCAT 2 cut(s) 550, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.