Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0017696

Physical Location & Seq

Genomic Coordinates Forward (+)

27992290 .. 27996088

Gene Structure 3799 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G273800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGAGACTGATCCTCCAAAGCAAACCAAAGCCACGGACAAGGAGACTGATCCTCCGAAACAAACCAAAGCCACGGACAAGGAGACTGATCCTCCGAAACAATCCAAAGCCACGGACATGGAGACTAATCCTCCGAAGCAAGCCAAAGCCACGGACAAGGAGACTCATCCTCCAAAGCAATCCAAAGCCACGGACATGGAGACTAATCCTCCGAAGCAAGCCAAAGCCATGGACGTGGAGACTGATCCTCCGAAGCAAACCATAGCATCATCTCAAGACCATCTCCTCCAGGTCCCCATCTCGTCCGAGGTGAGGGCCAATTGGATGCATGACCTTCCCATGCACCTTCTTGAGAGCATACTATCAGACTTACCTGTAAAGGAATCAGTGTGCACTAGCTGCATGTGCAAATCGTGGCATGATAATTTGTGGAGTTTTGTTTCTGCAGATGACACCTCTAAGTCTACTGAATCAGACTTCAAAAAGTCACTGGAGAAACTTCTTCAGAATCCGGCGTATTTCCTTAACGTCAGGTTAAGATGCACAAAATTTAAAAGGCACTCCTTCCTGCATGAGTTTCTTCGGGAACTGCTGAAAAAGAAAGTAATCAACCTGGAAATCGCAGTGGATCGTCATGAGAGTTTGGGATTTTTCCCCATCCCACCCGAAGTCTTTACAAGCATGCATCTTTGGGTCCTGAAGATAGCAAGCGCCAGAATTATTCTGTCTCCTCCAGGGACTTTCACAAGGTTGAGCGCTATACAAGTTGATGTATCTCGGTCATCTCACGCATCTATCCTTGACTTCTACAAGTGGTGTCCGGTGCTCCAGGATCTGCAAATACAAGGAAAAATTATGAGACTTCCGGACAAAAAGCAGAAATTCCAAATAAGGTCTCAGAGTCTCCTTAGACTAAAAATTACACTGCATCTTCACAAGCTACAGCTTGATAGAGGACAAAAAGTTTACCTTTCTGCTGGAAACCTCTATGAGGTTGACATTGATGCCCCCCAACTAGAAGTTTTGGATATAGATGAATCAATTTTTGCCAAGTTTAAGATCGGAAATTTCAAGCATCTCCGAGAAGTTAAGCTTCAAAGTGGCTTCTACGAAGCTAAGAGACAGCTAGGAGAAGTGGGGGAGCTTCTCGTTCTTTCGCGAGGGGTATTTTATACGATTAACCAAGCATCATTATCTACTAGCTGCCTGGTTGTGGGAGATGGAGCTTTTGGGGCATTGAGTTTTACGTTTATGGACTATCTTCTTAGTGGTAATGAAGCTGCGATCCGGAACAAATTTCGAATGACATTTCCAGATTTCTATCATGTCACGAGCTTGAGATTGTGTATAGCAGATTCTTCCGGTTGGCTGTTCATGCCATACTTGCTCGCACACCTTCCTAATTTACAAAGATTGGATCTCGAAGTGGTAGATAGGGAAGAAGTTCGAAAGGGCAAGAAATTTAAGTGGAATCCCCCAAAAGATCATGTGGGATGGTGTTTGACAAGCGTTAAACACGTTCGAATGATTGGATTTGGGGAAATGATGGAAGAAGCCAGTCGAATATTAGATTTTGTGAGGGAGGCTGCTACCGAAGAACGCTTTGAAGAAATTACCATCACCTATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 PIP5K

Protein Analysis

544

Amino Acids

62.16

Weight (kDa)

7.99

Isoelectric Point (pI)

49.3

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017696)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g29801
prunus_persica	Prupe.1G273800_v2.0.a1 Prupe.1G285400_v2.0.a1
rosa_laevigata	RLG00000026075
rosa_rugosa	Rorug02G0357900 Rorug02G0358000
rosa_samantha	Rh1BG427400 Rh1DG460300 Rh1DG460400 Rh2AG407300 Rh2BG417800 Rh2CG393300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	464
AccII	CGCG	1 cut(s)	1159
AccIII	TCCGGA	2 cut(s)	865, 1287
AclWI	GGATC	8 cut(s)	5, 44, 83, 239, 636, 840, 1279, 1425
AcsI	RAATTY	5 cut(s)	548, 881, 1066, 1295, 1460
AcuI	CTGAAG	2 cut(s)	488, 719
AfeI	AGCGCT	1 cut(s)	757
AfiI	CCNNNNNNNGG	3 cut(s)	312, 991, 1213
AflIII	ACRYGT	1 cut(s)	1516
AgsI	TTSAA	4 cut(s)	481, 1072, 1097, 1607
AjiI	CACGTC	1 cut(s)	235
AjnI	CCWGG	5 cut(s)	288, 612, 733, 828, 1206
Alw21I	GWGCWC	2 cut(s)	395, 828
Alw44I	GTGCAC	1 cut(s)	391
AlwI	GGATC	8 cut(s)	5, 44, 83, 239, 636, 840, 1279, 1425
AlwNI	CAGNNNCTG	1 cut(s)	835
Aor13HI	TCCGGA	2 cut(s)	865, 1287
Aor51HI	AGCGCT	1 cut(s)	757
AoxI	GGCC	1 cut(s)	315
ApaLI	GTGCAC	1 cut(s)	391
ApeKI	GCWGC	4 cut(s)	399, 1203, 1280, 1586
ApoI	RAATTY	5 cut(s)	548, 881, 1066, 1295, 1460
Asp700I	GAANNNNTTC	1 cut(s)	1443
AspLEI	GCGC	2 cut(s)	713, 758
AspS9I	GGNCC	3 cut(s)	292, 315, 694
AsuHPI	GGTGA	2 cut(s)	322, 1612
AsuII	TTCGAA	3 cut(s)	1300, 1447, 1522
AvaII	GGWCC	2 cut(s)	292, 694
BaeGI	GKGCMC	1 cut(s)	395
BauI	CACGAG	1 cut(s)	1330
Bbv12I	GWGCWC	2 cut(s)	395, 828
BbvI	GCAGC	4 cut(s)	386, 1190, 1267, 1573
BccI	CCATC	7 cut(s)	288, 305, 665, 1214, 1488, 1540, 1625
BciT130I	CCWGG	5 cut(s)	290, 614, 735, 830, 1208
BfaI	CTAG	4 cut(s)	396, 1016, 1127, 1200
BfmI	CTRYAG	2 cut(s)	444, 941
BfoI	RGCGCY	2 cut(s)	714, 759
BisI	GCNGC	4 cut(s)	400, 1204, 1281, 1587
BlsI	GCNGC	4 cut(s)	401, 1205, 1282, 1588
Bme1390I	CCNGG	5 cut(s)	290, 614, 735, 830, 1208
Bme18I	GGWCC	2 cut(s)	292, 694
BmgBI	CACGTC	1 cut(s)	235
BmgT120I	GGNCC	3 cut(s)	292, 315, 694
BmiI	GGNNCC	2 cut(s)	294, 695
BmrFI	CCNGG	5 cut(s)	290, 614, 735, 830, 1208
BmsI	GCATC	9 cut(s)	275, 315, 530, 694, 800, 937, 994, 1084, 1196
BpmI	CTGGAG	4 cut(s)	272, 512, 717, 812
Bpu14I	TTCGAA	3 cut(s)	1300, 1447, 1522
BpuEI	CTTGAG	3 cut(s)	258, 371, 1357
BsaBI	GATNNNNATC	1 cut(s)	1320
BsaI	GGTCTC	1 cut(s)	900
BsaJI	CCNNGG	8 cut(s)	33, 72, 111, 150, 189, 228, 306, 734
BsaWI	WCCGGW	4 cut(s)	820, 865, 1287, 1361
Bsc4I	CCNNNNNNNGG	3 cut(s)	312, 991, 1213
Bse1I	ACTGG	2 cut(s)	495, 1557
Bse8I	GATNNNNATC	1 cut(s)	1320
BseAI	TCCGGA	2 cut(s)	865, 1287
BseBI	CCWGG	5 cut(s)	290, 614, 735, 830, 1208
BseDI	CCNNGG	8 cut(s)	33, 72, 111, 150, 189, 228, 306, 734
BseGI	GGATG	4 cut(s)	166, 330, 657, 1499
BseJI	GATNNNNATC	1 cut(s)	1320
BseLI	CCNNNNNNNGG	3 cut(s)	312, 991, 1213
BseMII	CTCAG	1 cut(s)	911
BseNI	ACTGG	2 cut(s)	495, 1557
BseRI	GAGGAG	2 cut(s)	275, 720
BseSI	GKGCMC	1 cut(s)	395
BseXI	GCAGC	4 cut(s)	386, 1190, 1267, 1573
Bsh1236I	CGCG	1 cut(s)	1159
BshFI	GGCC	1 cut(s)	317
BsiHKAI	GWGCWC	2 cut(s)	395, 828
BsiSI	CCGG	5 cut(s)	512, 821, 866, 1288, 1362
BslFI	GGGAC	2 cut(s)	278, 751
BslI	CCNNNNNNNGG	3 cut(s)	312, 991, 1213
BsmFI	GGGAC	2 cut(s)	278, 751
BsnI	GGCC	1 cut(s)	317
Bso31I	GGTCTC	1 cut(s)	900
Bsp119I	TTCGAA	3 cut(s)	1300, 1447, 1522
Bsp1286I	GDGCHC	2 cut(s)	395, 828
Bsp13I	TCCGGA	2 cut(s)	865, 1287
Bsp19I	CCATGG	1 cut(s)	228
Bsp68I	TCGCGA	1 cut(s)	1159
BspANI	GGCC	1 cut(s)	317
BspCNI	CTCAG	1 cut(s)	910
BspEI	TCCGGA	2 cut(s)	865, 1287
BspFNI	CGCG	1 cut(s)	1159
BspHI	TCATGA	1 cut(s)	634
BspLI	GGNNCC	2 cut(s)	294, 695
BspMAI	CTGCAG	1 cut(s)	448
BspPI	GGATC	8 cut(s)	5, 44, 83, 239, 636, 840, 1279, 1425
BspT104I	TTCGAA	3 cut(s)	1300, 1447, 1522
BspTNI	GGTCTC	1 cut(s)	900
BsrI	ACTGG	2 cut(s)	495, 1557
BssECI	CCNNGG	8 cut(s)	33, 72, 111, 150, 189, 228, 306, 734
BssSI	CACGAG	1 cut(s)	1330
BssT1I	CCWWGG	1 cut(s)	228
Bst2BI	CACGAG	1 cut(s)	1330
Bst2UI	CCWGG	5 cut(s)	290, 614, 735, 830, 1208
BstAPI	GCANNNNNTGC	1 cut(s)	399
BstBI	TTCGAA	3 cut(s)	1300, 1447, 1522
BstC8I	GCNNGC	5 cut(s)	141, 219, 683, 709, 1389
BstDEI	CTNAG	5 cut(s)	459, 897, 908, 1116, 1265
BstDSI	CCRYGG	6 cut(s)	33, 72, 111, 150, 189, 228
BstENI	CCTNNNNNAGG	1 cut(s)	989
BstF5I	GGATG	4 cut(s)	166, 330, 657, 1499
BstFNI	CGCG	1 cut(s)	1159
BstH2I	RGCGCY	2 cut(s)	714, 759
BstHHI	GCGC	2 cut(s)	713, 758
BstMWI	GCNNNNNNNGC	5 cut(s)	399, 405, 1232, 1375, 1384
BstNI	CCWGG	5 cut(s)	290, 614, 735, 830, 1208
BstNSI	RCATGY	2 cut(s)	406, 685
BstSCI	CCNGG	5 cut(s)	288, 612, 733, 828, 1206
BstSFI	CTRYAG	2 cut(s)	444, 941
BstSLI	GKGCMC	1 cut(s)	395
BstUI	CGCG	1 cut(s)	1159
BstV1I	GCAGC	4 cut(s)	386, 1190, 1267, 1573
BstX2I	RGATCY	2 cut(s)	832, 1417
BstXI	CCANNNNNNTGG	4 cut(s)	118, 196, 229, 235
BstYI	RGATCY	2 cut(s)	832, 1417
BsuRI	GGCC	1 cut(s)	317
BtgI	CCRYGG	6 cut(s)	33, 72, 111, 150, 189, 228
BtrI	CACGTC	1 cut(s)	235
BtsCI	GGATG	4 cut(s)	166, 330, 657, 1499
BtsI	GCAGTG	2 cut(s)	630, 923
BtsIMutI	CAGTG	4 cut(s)	393, 488, 630, 923
BtuMI	TCGCGA	1 cut(s)	1159
Cac8I	GCNNGC	5 cut(s)	141, 219, 683, 709, 1389
CaiI	CAGNNNCTG	1 cut(s)	835
CciI	TCATGA	1 cut(s)	634
CfoI	GCGC	2 cut(s)	713, 758
Cfr13I	GGNCC	3 cut(s)	292, 315, 694
DdeI	CTNAG	5 cut(s)	459, 897, 908, 1116, 1265
DraI	TTTAAA	1 cut(s)	553
Eco130I	CCWWGG	1 cut(s)	228
Eco31I	GGTCTC	1 cut(s)	900
Eco47I	GGWCC	2 cut(s)	292, 694
Eco47III	AGCGCT	1 cut(s)	757
Eco57I	CTGAAG	2 cut(s)	488, 719
EcoNI	CCTNNNNNAGG	1 cut(s)	989
EcoO109I	RGGNCCY	2 cut(s)	292, 694
EcoRII	CCWGG	5 cut(s)	288, 612, 733, 828, 1206
EcoT14I	CCWWGG	1 cut(s)	228
EcoT22I	ATGCAT	2 cut(s)	330, 687
ErhI	CCWWGG	1 cut(s)	228
FalI	AAGNNNNNCTT	4 cut(s)	954, 986, 1077, 1109
FaqI	GGGAC	2 cut(s)	278, 751
FblI	GTMKAC	1 cut(s)	464
Fnu4HI	GCNGC	4 cut(s)	400, 1204, 1281, 1587
FokI	GGATG	4 cut(s)	153, 337, 644, 1506
Fsp4HI	GCNGC	4 cut(s)	400, 1204, 1281, 1587
FspBI	CTAG	4 cut(s)	396, 1016, 1127, 1200
GlaI	GCGC	2 cut(s)	712, 757
GluI	GCNGC	4 cut(s)	400, 1204, 1281, 1587
GsuI	CTGGAG	4 cut(s)	272, 512, 717, 812
HaeII	RGCGCY	2 cut(s)	714, 759
HaeIII	GGCC	1 cut(s)	317
HapII	CCGG	5 cut(s)	512, 821, 866, 1288, 1362
HhaI	GCGC	2 cut(s)	713, 758
Hin6I	GCGC	2 cut(s)	711, 756
HinP1I	GCGC	2 cut(s)	711, 756
HincII	GTYRAC	1 cut(s)	997
HindII	GTYRAC	1 cut(s)	997
HindIII	AAGCTT	1 cut(s)	1091
HinfI	GANTC	8 cut(s)	163, 383, 470, 508, 901, 1037, 1355, 1471
HpaII	CCGG	5 cut(s)	512, 821, 866, 1288, 1362
HphI	GGTGA	2 cut(s)	322, 1612
Hpy166II	GTNNAC	4 cut(s)	393, 465, 967, 997
Hpy8I	GTNNAC	4 cut(s)	393, 465, 967, 997
HpyAV	CCTTC	4 cut(s)	344, 356, 574, 1406
HpyCH4IV	ACGT	4 cut(s)	234, 528, 1247, 1518
HpyF10VI	GCNNNNNNNGC	5 cut(s)	399, 405, 1232, 1375, 1384
HpyF3I	CTNAG	5 cut(s)	459, 897, 908, 1116, 1265
HpySE526I	ACGT	4 cut(s)	234, 528, 1247, 1518
HspAI	GCGC	2 cut(s)	711, 756
Kpn2I	TCCGGA	2 cut(s)	865, 1287
LmnI	GCTCC	3 cut(s)	831, 1141, 1223
Lsp1109I	GCAGC	4 cut(s)	386, 1190, 1267, 1573
LweI	GCATC	9 cut(s)	275, 315, 530, 694, 800, 937, 994, 1084, 1196
MaeI	CTAG	4 cut(s)	396, 1016, 1127, 1200
MaeII	ACGT	4 cut(s)	234, 528, 1247, 1518
MaeIII	GTNAC	2 cut(s)	486, 1327
MfeI	CAATTG	1 cut(s)	319
MflI	RGATCY	2 cut(s)	832, 1417
MhlI	GDGCHC	2 cut(s)	395, 828
MlyI	GAGTC	2 cut(s)	157, 910
Mph1103I	ATGCAT	2 cut(s)	330, 687
MroI	TCCGGA	2 cut(s)	865, 1287
MroXI	GAANNNNTTC	1 cut(s)	1443
MseI	TTAA	8 cut(s)	525, 536, 552, 1056, 1089, 1179, 1464, 1512
MslI	CAYNNNNRTG	3 cut(s)	116, 194, 233
MspI	CCGG	5 cut(s)	512, 821, 866, 1288, 1362
MspR9I	CCNGG	5 cut(s)	290, 614, 735, 830, 1208
MunI	CAATTG	1 cut(s)	319
MvaI	CCWGG	5 cut(s)	290, 614, 735, 830, 1208
MvnI	CGCG	1 cut(s)	1159
MwoI	GCNNNNNNNGC	5 cut(s)	399, 405, 1232, 1375, 1384
NcoI	CCATGG	1 cut(s)	228
NlaIV	GGNNCC	2 cut(s)	294, 695
NmuCI	GTSAC	2 cut(s)	486, 1327
NruI	TCGCGA	1 cut(s)	1159
NsiI	ATGCAT	2 cut(s)	330, 687
NspI	RCATGY	2 cut(s)	406, 685
NspV	TTCGAA	3 cut(s)	1300, 1447, 1522
PaeI	GCATGC	1 cut(s)	685
PagI	TCATGA	1 cut(s)	634
PdmI	GAANNNNTTC	1 cut(s)	1443
PfeI	GAWTC	6 cut(s)	383, 470, 508, 1037, 1355, 1471
PfoI	TCCNGGA	1 cut(s)	828
PkrI	GCNGC	4 cut(s)	401, 1205, 1282, 1588
PleI	GAGTC	2 cut(s)	157, 909
PpsI	GAGTC	2 cut(s)	157, 909
PpuMI	RGGWCCY	2 cut(s)	292, 694
Psp5II	RGGWCCY	2 cut(s)	292, 694
Psp6I	CCWGG	5 cut(s)	288, 612, 733, 828, 1206
PspGI	CCWGG	5 cut(s)	288, 612, 733, 828, 1206
PspN4I	GGNNCC	2 cut(s)	294, 695
PspPI	GGNCC	3 cut(s)	292, 315, 694
PspPPI	RGGWCCY	2 cut(s)	292, 694
PstI	CTGCAG	1 cut(s)	448
PstNI	CAGNNNCTG	1 cut(s)	835
PsuI	RGATCY	2 cut(s)	832, 1417
RruI	TCGCGA	1 cut(s)	1159
RseI	CAYNNNNRTG	3 cut(s)	116, 194, 233
SaqAI	TTAA	8 cut(s)	525, 536, 552, 1056, 1089, 1179, 1464, 1512
SatI	GCNGC	4 cut(s)	400, 1204, 1281, 1587
Sau96I	GGNCC	3 cut(s)	292, 315, 694
SchI	GAGTC	2 cut(s)	157, 910
ScrFI	CCNGG	5 cut(s)	290, 614, 735, 830, 1208
SduI	GDGCHC	2 cut(s)	395, 828
SfaNI	GCATC	9 cut(s)	275, 315, 530, 694, 800, 937, 994, 1084, 1196
SfcI	CTRYAG	2 cut(s)	444, 941
SfuI	TTCGAA	3 cut(s)	1300, 1447, 1522
SinI	GGWCC	2 cut(s)	292, 694
SmiMI	CAYNNNNRTG	3 cut(s)	116, 194, 233
SmlI	CTYRAG	3 cut(s)	273, 350, 1336
SmoI	CTYRAG	3 cut(s)	273, 350, 1336
SphI	GCATGC	1 cut(s)	685
SspI	AATATT	1 cut(s)	1566
SspMI	CTAG	4 cut(s)	396, 1016, 1127, 1200
StyD4I	CCNGG	5 cut(s)	288, 612, 733, 828, 1206
StyI	CCWWGG	1 cut(s)	228
TaiI	ACGT	4 cut(s)	237, 531, 1250, 1521
TaqI	TCGA	5 cut(s)	1300, 1422, 1447, 1522, 1561
TaqII	GACCGA	1 cut(s)	768
TfiI	GAWTC	6 cut(s)	383, 470, 508, 1037, 1355, 1471
Tru1I	TTAA	8 cut(s)	525, 536, 552, 1056, 1089, 1179, 1464, 1512
Tru9I	TTAA	8 cut(s)	525, 536, 552, 1056, 1089, 1179, 1464, 1512
TscAI	CASTG	4 cut(s)	393, 495, 630, 930
TseFI	GTSAC	2 cut(s)	486, 1327
TseI	GCWGC	4 cut(s)	399, 1203, 1280, 1586
Tsp45I	GTSAC	2 cut(s)	486, 1327
TspDTI	ATGAA	3 cut(s)	1050, 1290, 1363
TspGWI	ACGGA	5 cut(s)	50, 89, 128, 167, 206
TspRI	CASTG	4 cut(s)	393, 495, 630, 930
VneI	GTGCAC	1 cut(s)	391
VpaK11BI	GGWCC	2 cut(s)	292, 694
XagI	CCTNNNNNAGG	1 cut(s)	989
XapI	RAATTY	5 cut(s)	548, 881, 1066, 1295, 1460
XceI	RCATGY	2 cut(s)	406, 685
XmiI	GTMKAC	1 cut(s)	464
XmnI	GAANNNNTTC	1 cut(s)	1443
XspI	CTAG	4 cut(s)	396, 1016, 1127, 1200
Zsp2I	ATGCAT	2 cut(s)	330, 687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.