Rh1BG427400

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0017696
Physical Location & Seq
Reverse (-)
54530090 .. 54531636
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG427400.1

Sequence Viewer

Length: 1380 bp
ATGGCTGACAAAGCTAGGATCAGCGAGCTACCTGACGATATCAGCTTAAAGATCCTCTCCTTGCTACCAGGCAAAGATGCGGTAGCAACAGCAATTCTATCACATAAATGGAAAGACCTATATAAACCGCAAGGTGAAGTGAGGTTTGAATTCAACTCAACGCATGAGGACAGAGAAACTCTATACAACTCTATCAAGGAGGAGTTGCTTATTACAGGTGTACCACCCCTACTTAGGTTTGTCTTACAATGCAACTTGTACAAGGAGCGTCGCCCAATTGAGCATATGCTCCATGCAGCAACTAAAAATCGCCTTGTGGTGGAGATGGAAGTGTGTGTCAAACCAGACAAAGAGAAAGGTGTGTTCCCACTACCTGAATTTGTGCTTACAAGCCAACATCTGAAGTACTTAAGATTGTGTGGGGTGAGCGTGAGAGTTCCAGATGGGACACCTGTTCTCCAATCTTTGAAGAGGTTACAAGTCGATAAATCATTAAAGAGTGACATTTCCCTCCAGTACCTCTATGCCAAATGTCCTGTTCTTCAGGAGCTCACAATGGAAGGAAATATCGATGACCTACCTCGTGAGTCTGACAAGCGTGTGTTTGATGTATATTCCCCTAGTCTGAAAACTTTGCAGATAAAACTCGAGACAGATTTTGAGTTTGGTCTCACAGGTCATCATGCACCAGACCCCATATACTCATTCTGCATATATGCACCAAAGCTGGAGAATCTCTTGATAGAAGAGAACATGTTAGCTTGCTACAGAGTCCACAACTTGGAAACTACAGTTGAAGCCAGGCTTCAGATTGGATGGCGAGCTGTGGGCTATGAATTCACAGATCCTAAGTCTCTAAATGATGCTTTCAGCCTTTTGAGAGGCCTAACCAATGTGCAGTCTCTGACCCTTTACGACCATACTACAGCGGCTGCTGGATTTATTCTTAAGGAGATGTATCAAGCTGAATTCATTGCAGCAGAGAGGGCAGGACATGAAATGTCTGATGCTGACAATAAGGACTGGAGCCTGTATAGAAACGCACTACCTATCTTTTGTCTATTAACTGAGTTAAATTTACATGTATCAAATCATTTTGGATGTAGAACGGTCATGTATCTACTTGACCAGACACCAGCTTTGAAGCTCCTAGTCATACAGATGGTAGAGAGAGATAATGTAGAGAGGGATCAAAGGTTTGCATGGACGCCACCACCAGTCACACCTGAATGCGTGAGAAAAAACAGCCTTAAAAAAGTTCAGCTGAAAGGGTTTCTGGGGCATGAAGAGGAATGGTCACCAATGATTAACTATTTCCAGGACATTGCAAGGTCAACTGAATCATCACAGCCAGAGGATGCATTTCTTCTTATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

459

Amino Acids

52.67

Weight (kDa)

5.64

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 7 - 40 5.4e-06 F-box domain
LRR_At5g56370 PF24758 106 - 254 3.1e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 781
AciI CCGC 3 cut(s) 80, 128, 929
AclWI GGATC 4 cut(s) 26, 46, 839, 1197
AcsI RAATTY 5 cut(s) 149, 377, 836, 968, 1075
AcuI CTGAAG 3 cut(s) 422, 527, 791
AcyI GRCGYC 1 cut(s) 1208
AfaI GTAC 4 cut(s) 222, 260, 407, 518
AfiI CCNNNNNNNGG 3 cut(s) 234, 319, 781
AflII CTTAAG 2 cut(s) 409, 947
AflIII ACRYGT 2 cut(s) 753, 1081
AgsI TTSAA 5 cut(s) 149, 154, 469, 797, 1144
AjnI CCWGG 3 cut(s) 67, 800, 1317
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 4 cut(s) 644, 674, 858, 906
AlwI GGATC 4 cut(s) 26, 46, 839, 1197
AlwNI CAGNNNCTG 2 cut(s) 904, 932
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 1 cut(s) 883
ApeKI GCWGC 3 cut(s) 296, 932, 977
ApoI RAATTY 5 cut(s) 149, 377, 836, 968, 1075
ArsI GACNNNNNNTTYG 2 cut(s) 1123, 1155
AsuHPI GGTGA 3 cut(s) 146, 436, 1290
AvaI CYCGRG 1 cut(s) 647
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanII GRGCYC 1 cut(s) 552
BauI CACGAG 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 3 cut(s) 308, 919, 989
BccI CCATC 4 cut(s) 319, 437, 810, 1156
BciT130I CCWGG 3 cut(s) 69, 802, 1319
BcoDI GTCTC 4 cut(s) 644, 674, 858, 906
BfaI CTAG 3 cut(s) 15, 621, 1151
BfmI CTRYAG 3 cut(s) 766, 789, 924
BfrI CTTAAG 2 cut(s) 409, 947
BisI GCNGC 4 cut(s) 297, 930, 933, 978
BlsI GCNGC 4 cut(s) 298, 931, 934, 979
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 3 cut(s) 69, 802, 1319
BmeT110I CYCGRG 1 cut(s) 647
BmiI GGNNCC 1 cut(s) 1028
BmrFI CCNGG 3 cut(s) 69, 802, 1319
BmsI GCATC 4 cut(s) 67, 853, 997, 1348
BpmI CTGGAG 3 cut(s) 497, 749, 1045
Bsa29I ATCGAT 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 1208
BsaI GGTCTC 1 cut(s) 674
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 319, 781
Bse1I ACTGG 3 cut(s) 514, 1028, 1217
Bse3DI GCAATG 2 cut(s) 972, 1323
BseBI CCWGG 3 cut(s) 69, 802, 1319
BseCI ATCGAT 1 cut(s) 570
BseGI GGATG 3 cut(s) 821, 1106, 1363
BseLI CCNNNNNNNGG 3 cut(s) 234, 319, 781
BseMI GCAATG 2 cut(s) 972, 1323
BseMII CTCAG 1 cut(s) 1059
BseNI ACTGG 3 cut(s) 514, 1028, 1217
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 3 cut(s) 308, 919, 989
BsgI GTGCAG 1 cut(s) 917
BshFI GGCC 1 cut(s) 885
BshVI ATCGAT 1 cut(s) 570
BsiHKAI GWGCWC 1 cut(s) 552
BsiHKCI CYCGRG 1 cut(s) 647
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 3 cut(s) 234, 319, 781
BsmAI GTCTC 4 cut(s) 644, 674, 858, 906
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 1 cut(s) 1235
BsnI GGCC 1 cut(s) 885
Bso31I GGTCTC 1 cut(s) 674
BsoBI CYCGRG 1 cut(s) 647
Bsp1286I GDGCHC 1 cut(s) 552
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 4 cut(s) 18, 51, 844, 1189
BspACI CCGC 3 cut(s) 80, 128, 929
BspANI GGCC 1 cut(s) 885
BspCNI CTCAG 1 cut(s) 1060
BspDI ATCGAT 1 cut(s) 570
BspLI GGNNCC 1 cut(s) 1028
BspPI GGATC 4 cut(s) 26, 46, 839, 1197
BspTI CTTAAG 2 cut(s) 409, 947
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 2 cut(s) 972, 1323
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 3 cut(s) 514, 1028, 1217
BssMI GATC 4 cut(s) 18, 51, 844, 1189
BssNI GRCGYC 1 cut(s) 1208
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 3 cut(s) 69, 802, 1319
Bst4CI ACNGT 2 cut(s) 793, 1111
Bst6I CTCTTC 3 cut(s) 464, 741, 1281
BstACI GRCGYC 1 cut(s) 1208
BstAFI CTTAAG 2 cut(s) 409, 947
BstAUI TGTACA 1 cut(s) 258
BstC8I GCNNGC 3 cut(s) 26, 763, 822
BstDEI CTNAG 3 cut(s) 233, 849, 1068
BstEII GGTNACC 1 cut(s) 1296
BstF5I GGATG 3 cut(s) 821, 1106, 1363
BstKTI GATC 4 cut(s) 21, 54, 847, 1192
BstMAI GTCTC 4 cut(s) 644, 674, 858, 906
BstMBI GATC 4 cut(s) 18, 51, 844, 1189
BstMWI GCNNNNNNNGC 2 cut(s) 11, 986
BstNI CCWGG 3 cut(s) 69, 802, 1319
BstNSI RCATGY 2 cut(s) 757, 1085
BstPI GGTNACC 1 cut(s) 1296
BstSCI CCNGG 3 cut(s) 67, 800, 1317
BstSFI CTRYAG 3 cut(s) 766, 789, 924
BstV1I GCAGC 3 cut(s) 308, 919, 989
BstX2I RGATCY 2 cut(s) 51, 844
BstYI RGATCY 2 cut(s) 51, 844
Bsu15I ATCGAT 1 cut(s) 570
BsuRI GGCC 1 cut(s) 885
BsuTUI ATCGAT 1 cut(s) 570
BtsCI GGATG 3 cut(s) 821, 1106, 1363
Cac8I GCNNGC 3 cut(s) 26, 763, 822
CaiI CAGNNNCTG 2 cut(s) 904, 932
ClaI ATCGAT 1 cut(s) 570
CseI GACGC 2 cut(s) 257, 1216
Csp6I GTAC 4 cut(s) 221, 259, 406, 517
CviAII CATG 9 cut(s) 164, 293, 683, 754, 995, 1082, 1114, 1203, 1283
CviQI GTAC 4 cut(s) 221, 259, 406, 517
DdeI CTNAG 3 cut(s) 233, 849, 1068
DpnI GATC 4 cut(s) 20, 53, 846, 1191
DpnII GATC 4 cut(s) 18, 51, 844, 1189
Eam1104I CTCTTC 3 cut(s) 464, 741, 1281
EarI CTCTTC 3 cut(s) 464, 741, 1281
Ecl136II GAGCTC 1 cut(s) 550
Eco147I AGGCCT 1 cut(s) 885
Eco24I GRGCYC 1 cut(s) 552
Eco31I GGTCTC 1 cut(s) 674
Eco32I GATATC 1 cut(s) 40
Eco53kI GAGCTC 1 cut(s) 550
Eco57I CTGAAG 3 cut(s) 422, 527, 791
Eco88I CYCGRG 1 cut(s) 647
Eco91I GGTNACC 1 cut(s) 1296
EcoICRI GAGCTC 1 cut(s) 550
EcoO65I GGTNACC 1 cut(s) 1296
EcoRI GAATTC 3 cut(s) 149, 836, 968
EcoRII CCWGG 3 cut(s) 67, 800, 1317
EcoRV GATATC 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 1363
EcoT38I GRGCYC 1 cut(s) 552
FaeI CATG 9 cut(s) 167, 296, 686, 757, 998, 1085, 1117, 1206, 1286
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 1 cut(s) 460
FatI CATG 9 cut(s) 163, 292, 682, 753, 994, 1081, 1113, 1202, 1282
FauNDI CATATG 1 cut(s) 285
Fnu4HI GCNGC 4 cut(s) 297, 930, 933, 978
FokI GGATG 3 cut(s) 828, 1113, 1370
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 4 cut(s) 297, 930, 933, 978
FspBI CTAG 3 cut(s) 15, 621, 1151
GluI GCNGC 4 cut(s) 297, 930, 933, 978
GsuI CTGGAG 3 cut(s) 497, 749, 1045
HaeIII GGCC 1 cut(s) 885
HgaI GACGC 2 cut(s) 257, 1216
Hin1I GRCGYC 1 cut(s) 1208
Hin1II CATG 9 cut(s) 167, 296, 686, 757, 998, 1085, 1117, 1206, 1286
HincII GTYRAC 1 cut(s) 1335
HindII GTYRAC 1 cut(s) 1335
HinfI GANTC 4 cut(s) 587, 733, 771, 1340
HphI GGTGA 3 cut(s) 146, 436, 1290
Hpy166II GTNNAC 3 cut(s) 221, 775, 1335
Hpy188I TCNGA 6 cut(s) 402, 592, 627, 810, 906, 1006
Hpy188III TCNNGA 5 cut(s) 440, 545, 584, 649, 739
Hpy8I GTNNAC 3 cut(s) 221, 775, 1335
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 2 cut(s) 793, 1111
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 986
HpyF3I CTNAG 3 cut(s) 233, 849, 1068
Hsp92I GRCGYC 1 cut(s) 1208
Hsp92II CATG 9 cut(s) 167, 296, 686, 757, 998, 1085, 1117, 1206, 1286
Kzo9I GATC 4 cut(s) 18, 51, 844, 1189
LmnI GCTCC 5 cut(s) 265, 294, 547, 1026, 1152
Lsp1109I GCAGC 3 cut(s) 308, 919, 989
LweI GCATC 4 cut(s) 67, 853, 997, 1348
MaeI CTAG 3 cut(s) 15, 621, 1151
MaeIII GTNAC 4 cut(s) 474, 500, 1219, 1296
MalI GATC 4 cut(s) 20, 53, 846, 1191
MboI GATC 4 cut(s) 18, 51, 844, 1189
MboII GAAGA 5 cut(s) 481, 533, 758, 1298, 1358
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 2 cut(s) 51, 844
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 7 cut(s) 93, 149, 276, 377, 836, 968, 1075
MlyI GAGTC 2 cut(s) 596, 780
Mph1103I ATGCAT 1 cut(s) 1363
MseI TTAA 8 cut(s) 47, 410, 494, 948, 1064, 1073, 1251, 1308
MslI CAYNNNNRTG 3 cut(s) 106, 1160, 1228
MspA1I CMGCKG 2 cut(s) 929, 1264
MspCI CTTAAG 2 cut(s) 409, 947
MspR9I CCNGG 3 cut(s) 69, 802, 1319
MunI CAATTG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 1235
MvaI CCWGG 3 cut(s) 69, 802, 1319
MwoI GCNNNNNNNGC 2 cut(s) 11, 986
NdeI CATATG 1 cut(s) 285
NdeII GATC 4 cut(s) 18, 51, 844, 1189
NlaIII CATG 9 cut(s) 167, 296, 686, 757, 998, 1085, 1117, 1206, 1286
NlaIV GGNNCC 1 cut(s) 1028
NmuCI GTSAC 3 cut(s) 500, 1219, 1296
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 2 cut(s) 757, 1085
PaeR7I CTCGAG 1 cut(s) 647
PceI AGGCCT 1 cut(s) 885
PciI ACATGT 2 cut(s) 753, 1081
PctI GAATGC 1 cut(s) 1235
PfeI GAWTC 2 cut(s) 733, 1340
PflMI CCANNNNNTGG 1 cut(s) 781
PfoI TCCNGGA 1 cut(s) 1317
PkrI GCNGC 4 cut(s) 298, 931, 934, 979
PleI GAGTC 2 cut(s) 595, 779
PpsI GAGTC 2 cut(s) 595, 779
PscI ACATGT 2 cut(s) 753, 1081
Psp124BI GAGCTC 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 67, 800, 1317
PspEI GGTNACC 1 cut(s) 1296
PspGI CCWGG 3 cut(s) 67, 800, 1317
PspN4I GGNNCC 1 cut(s) 1028
PstNI CAGNNNCTG 2 cut(s) 904, 932
PsuI RGATCY 2 cut(s) 51, 844
PvuII CAGCTG 1 cut(s) 1264
RsaI GTAC 4 cut(s) 222, 260, 407, 518
RsaNI GTAC 4 cut(s) 221, 259, 406, 517
RseI CAYNNNNRTG 3 cut(s) 106, 1160, 1228
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 8 cut(s) 47, 410, 494, 948, 1064, 1073, 1251, 1308
SatI GCNGC 4 cut(s) 297, 930, 933, 978
Sau3AI GATC 4 cut(s) 18, 51, 844, 1189
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 2 cut(s) 596, 780
ScrFI CCNGG 3 cut(s) 69, 802, 1319
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 4 cut(s) 67, 853, 997, 1348
SfcI CTRYAG 3 cut(s) 766, 789, 924
Sfr274I CTCGAG 1 cut(s) 647
SlaI CTCGAG 1 cut(s) 647
SmiMI CAYNNNNRTG 3 cut(s) 106, 1160, 1228
SmlI CTYRAG 3 cut(s) 409, 647, 947
SmoI CTYRAG 3 cut(s) 409, 647, 947
Sse9I AATT 7 cut(s) 93, 149, 276, 377, 836, 968, 1075
SseBI AGGCCT 1 cut(s) 885
SsiI CCGC 3 cut(s) 80, 128, 929
SspMI CTAG 3 cut(s) 15, 621, 1151
SstI GAGCTC 1 cut(s) 552
StuI AGGCCT 1 cut(s) 885
StyD4I CCNGG 3 cut(s) 67, 800, 1317
TaaI ACNGT 2 cut(s) 793, 1111
TaqI TCGA 3 cut(s) 483, 570, 648
TasI AATT 7 cut(s) 93, 149, 276, 377, 836, 968, 1075
TatI WGTACW 2 cut(s) 258, 405
TauI GCSGC 1 cut(s) 932
TfiI GAWTC 2 cut(s) 733, 1340
Tru1I TTAA 8 cut(s) 47, 410, 494, 948, 1064, 1073, 1251, 1308
Tru9I TTAA 8 cut(s) 47, 410, 494, 948, 1064, 1073, 1251, 1308
TseFI GTSAC 3 cut(s) 500, 1219, 1296
TseI GCWGC 3 cut(s) 296, 932, 977
Tsp45I GTSAC 3 cut(s) 500, 1219, 1296
TspDTI ATGAA 4 cut(s) 849, 961, 1011, 1299
Van91I CCANNNNNTGG 1 cut(s) 781
Vha464I CTTAAG 2 cut(s) 409, 947
XapI RAATTY 5 cut(s) 149, 377, 836, 968, 1075
XceI RCATGY 2 cut(s) 757, 1085
XhoI CTCGAG 1 cut(s) 647
XspI CTAG 3 cut(s) 15, 621, 1151
ZrmI AGTACT 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.