Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0007514

Physical Location & Seq

Genomic Coordinates Forward (+)

44830700 .. 44834704

Gene Structure 4005 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G548100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGGTCTTCTCAACTCAGCTTCGGTTGTTGTTTGTGTTCTTGTTTGTCAACTATTTGCCTTGCATTTGGGTGATCTTGTAAGCACTGTCTCAGAGAAGTACATATCAGCAATAGGAGACCCAGGAATGAAGAACCCAAATGTGAGAGTTGCATTGGAAGCTTGGAATTTCTGCAATGAAGTTGGAATGGAAGCACCCAACATGGGCAGCCCCAGGCTGGCCGATTGTGCTGATATTGATTGCTCTCTTACTGATCATTTGGATAGGACCTCCCTAGGTATGGAAAGTAAGTGTGTGATTCATCACAGAGTAAATGAATCAGACAACAACTTACGGGCGGGTGATAATTTTCCTGTAAGGGATTTTAAGCCATACACAGACCCTGACTTATATGCAAAGGAAAAGGAACTGTATCTTGCTTCTCTATGTGAGGTTCAGAATTCATCAGATCCATGGCAATTTTGGATGGTCATGCTTAAGAACGGAAATTTTGACAAAAACACTACTCTTTGTCCTGAAAATGGCAAGCCGGTAAGCAGAATTGTTACGGATAGGAATTTTCCATGTTTTGGTCAAGGTTGTATGAATCAGCCACTTGTCTACCATAACTACTCAAGATTGATTTCTTATGGTGACCGAGCAGTGTCTTTAATTGGAGGATTTTATGGAACTTATGATCTTGATGCTGATTTGAGCAAAGGTATAGGGAAGAACTCCTACTTTTCAGTCTCATGGCAGAAAAACTTGGGCACAGGAAGTTGGATTTTCTCGCATAGATTGACAACATCTTCAAAGTACCCCTGGCTTATGTTGTATCTTCGTGCAGATGCAACAAAAGGACTTAATGGTGGTTATCACTATGATGGTCGTGGCATCATGAGAAAGCTGCCAGTCTCTCCACATTTTAAAGTAAGATTGACACTAGATGTTAAACGTGGAGGAGGCTCCAACAGTCAATTTTATCTCCTTGACATAGGAAGCTGTTGGAAGAACAATGGAAAACCATGTGATGGTAATGTTCTGACAGATGTGACTAGATACAGTGAAATGATAATAAATCCAGAAACTACAAGCTGGTGTCGGCCGGATAACCTTGTTTCCTGCCCTCCTTACCATGTTACTCGTACTGGTGATATAATATATCGAAACGAAACATCTCGGTTTCCATATTCTGCTTATCATCTCTACTGCACACCTGGAAATGCTGAATTTCTGGAGAAACCATATGACATATGTGACCCATATAGCAATCCACAAGCACAAGAACTGGTACAAATTCTTCCACATCCTGAATGGGCTGTACATGGCTATCCTGCTAAACAAGGAGATGGATGGGTTGGAGACCCCAGGACATGGGAGCTTGATGTGGGGGCCCTCTCTAGCCAGTTGTATTTCTACCAGGACCCAGGAACAAAACCAGCACGGAGAGTATGGTCTTCGATTAATGTTGGTACAGAAATATATGTCAGTAGCCCAGGAGAGACTGCGGAATGGACTGTAAGTGATTTTGATGTGCTCGTTCCTGAAGGTGTTGAAGATGGTTCTAGCTCTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

519

Amino Acids

57.98

Weight (kDa)

5.43

Isoelectric Point (pI)

29.02

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007514)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g32760
malus_domestica	MD15G1400500.v1.1
prunus_persica	Prupe.1G548100_v2.0.a1
pyrus_communis	pycom15g35910
rosa_chinensis	RchiOBHm_Chr7g0230431 RchiOBHm_Chr7g0230451
rosa_laevigata	RLG00000001493 RLG00000001495
rosa_multiflora	Rmu_sc0002541.1_g000025 Rmu_sc0002541.1_g000027 Rmu_sc0007103.1_g000011 Rmu_sc0007103.1_g000013
rosa_roxburghii	Rroxscaffold_3G00230150 Rroxscaffold_3G00230160
rosa_rugosa	Rorug07G0260500
rosa_samantha	Rh7AG411100 Rh7AG411600 Rh7AG411800 Rh7BG389700 Rh7CG429200 Rh7CG429300 Rh7DG407700 Rh7DG407900
rosa_wichuraiana	Rw7G034120 Rw7G034140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	3 cut(s)	569, 1010, 1353
AccI	GTMKAC	1 cut(s)	600
AciI	CCGC	2 cut(s)	338, 1487
AclWI	GGATC	1 cut(s)	443
AcoI	YGGCCR	2 cut(s)	219, 1082
AcsI	RAATTY	6 cut(s)	166, 439, 487, 556, 1208, 1275
AcuI	CTGAAG	1 cut(s)	1545
AfaI	GTAC	6 cut(s)	101, 797, 1126, 1272, 1302, 1453
AfiI	CCNNNNNNNGG	7 cut(s)	203, 217, 280, 521, 569, 1010, 1353
AflII	CTTAAG	1 cut(s)	476
AgsI	TTSAA	2 cut(s)	792, 1535
AjnI	CCWGG	8 cut(s)	121, 212, 800, 1195, 1346, 1398, 1405, 1474
AluBI	AGCT	7 cut(s)	20, 161, 886, 981, 1074, 1360, 1548
AluI	AGCT	7 cut(s)	20, 161, 886, 981, 1074, 1360, 1548
Alw21I	GWGCWC	1 cut(s)	1518
Alw26I	GTCTC	6 cut(s)	94, 111, 733, 898, 1335, 1475
AlwI	GGATC	1 cut(s)	443
AlwNI	CAGNNNCTG	1 cut(s)	383
AoxI	GGCC	3 cut(s)	219, 1082, 1371
ApaI	GGGCCC	1 cut(s)	1375
ApeKI	GCWGC	2 cut(s)	207, 886
ApoI	RAATTY	6 cut(s)	166, 439, 487, 556, 1208, 1275
AseI	ATTAAT	1 cut(s)	1443
AspA2I	CCTAGG	1 cut(s)	274
AspS9I	GGNCC	4 cut(s)	267, 1371, 1372, 1402
AsuHPI	GGTGA	4 cut(s)	83, 353, 644, 1142
AvaII	GGWCC	2 cut(s)	267, 1402
AvrII	CCTAGG	1 cut(s)	274
BaeGI	GKGCMC	2 cut(s)	752, 1375
BanII	GRGCYC	1 cut(s)	1375
BarI	GAAGNNNNNNTAC	4 cut(s)	701, 733, 1263, 1295
BbsI	GAAGAC	1 cut(s)	1428
Bbv12I	GWGCWC	1 cut(s)	1518
BbvI	GCAGC	2 cut(s)	219, 873
BccI	CCATC	6 cut(s)	460, 857, 1004, 1322, 1326, 1532
BciT130I	CCWGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
BclI	TGATCA	1 cut(s)	253
BcoDI	GTCTC	6 cut(s)	94, 111, 733, 898, 1335, 1475
BfaI	CTAG	5 cut(s)	275, 923, 1035, 1380, 1545
BfrI	CTTAAG	1 cut(s)	476
BisI	GCNGC	2 cut(s)	208, 887
BlnI	CCTAGG	1 cut(s)	274
BlsI	GCNGC	2 cut(s)	209, 888
Bme1390I	CCNGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
Bme18I	GGWCC	2 cut(s)	267, 1402
BmgT120I	GGNCC	4 cut(s)	267, 1371, 1372, 1402
BmiI	GGNNCC	4 cut(s)	946, 1372, 1373, 1404
BmrFI	CCNGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
BmsI	GCATC	3 cut(s)	673, 817, 882
BpiI	GAAGAC	1 cut(s)	1428
BpmI	CTGGAG	1 cut(s)	1235
BpuEI	CTTGAG	1 cut(s)	598
BsaI	GGTCTC	2 cut(s)	111, 1335
BsaJI	CCNNGG	8 cut(s)	121, 212, 274, 452, 800, 1346, 1405, 1474
Bsc4I	CCNNNNNNNGG	7 cut(s)	203, 217, 280, 521, 569, 1010, 1353
Bse118I	RCCGGY	1 cut(s)	529
Bse1I	ACTGG	4 cut(s)	890, 1132, 1272, 1384
Bse3DI	GCAATG	1 cut(s)	181
BseBI	CCWGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
BseDI	CCNNGG	8 cut(s)	121, 212, 274, 452, 800, 1346, 1405, 1474
BseGI	GGATG	3 cut(s)	471, 1285, 1337
BseLI	CCNNNNNNNGG	7 cut(s)	203, 217, 280, 521, 569, 1010, 1353
BseMI	GCAATG	1 cut(s)	181
BseMII	CTCAG	2 cut(s)	30, 105
BseNI	ACTGG	4 cut(s)	890, 1132, 1272, 1384
BseRI	GAGGAG	1 cut(s)	954
BseSI	GKGCMC	2 cut(s)	752, 1375
BseX3I	CGGCCG	1 cut(s)	1082
BseXI	GCAGC	2 cut(s)	219, 873
BsgI	GTGCAG	2 cut(s)	843, 1174
Bsh1285I	CGRYCG	1 cut(s)	1085
BshFI	GGCC	3 cut(s)	221, 1084, 1373
BsiEI	CGRYCG	1 cut(s)	1085
BsiHKAI	GWGCWC	1 cut(s)	1518
BsiSI	CCGG	2 cut(s)	530, 1085
BslI	CCNNNNNNNGG	7 cut(s)	203, 217, 280, 521, 569, 1010, 1353
BsmAI	GTCTC	6 cut(s)	94, 111, 733, 898, 1335, 1475
BsnI	GGCC	3 cut(s)	221, 1084, 1373
Bso31I	GGTCTC	2 cut(s)	111, 1335
Bsp120I	GGGCCC	1 cut(s)	1371
Bsp1286I	GDGCHC	3 cut(s)	752, 1375, 1518
Bsp1407I	TGTACA	1 cut(s)	1300
Bsp143I	GATC	4 cut(s)	73, 253, 448, 676
Bsp19I	CCATGG	1 cut(s)	452
BspACI	CCGC	2 cut(s)	338, 1487
BspANI	GGCC	3 cut(s)	221, 1084, 1373
BspCNI	CTCAG	2 cut(s)	29, 104
BspHI	TCATGA	1 cut(s)	876
BspLI	GGNNCC	4 cut(s)	946, 1372, 1373, 1404
BspPI	GGATC	1 cut(s)	443
BspTI	CTTAAG	1 cut(s)	476
BspTNI	GGTCTC	2 cut(s)	111, 1335
BsrDI	GCAATG	1 cut(s)	181
BsrFI	RCCGGY	1 cut(s)	529
BsrGI	TGTACA	1 cut(s)	1300
BsrI	ACTGG	4 cut(s)	890, 1132, 1272, 1384
BssAI	RCCGGY	1 cut(s)	529
BssECI	CCNNGG	8 cut(s)	121, 212, 274, 452, 800, 1346, 1405, 1474
BssMI	GATC	4 cut(s)	73, 253, 448, 676
BssT1I	CCWWGG	2 cut(s)	274, 452
Bst2UI	CCWGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
Bst4CI	ACNGT	5 cut(s)	88, 411, 953, 1043, 1498
BstAFI	CTTAAG	1 cut(s)	476
BstAUI	TGTACA	1 cut(s)	1300
BstC8I	GCNNGC	2 cut(s)	219, 527
BstDEI	CTNAG	2 cut(s)	16, 91
BstDSI	CCRYGG	1 cut(s)	452
BstEII	GGTNACC	1 cut(s)	632
BstF5I	GGATG	3 cut(s)	471, 1285, 1337
BstKTI	GATC	4 cut(s)	76, 256, 451, 679
BstMAI	GTCTC	6 cut(s)	94, 111, 733, 898, 1335, 1475
BstMBI	GATC	4 cut(s)	73, 253, 448, 676
BstMCI	CGRYCG	1 cut(s)	1085
BstMWI	GCNNNNNNNGC	2 cut(s)	158, 227
BstNI	CCWGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
BstPI	GGTNACC	1 cut(s)	632
BstSCI	CCNGG	8 cut(s)	121, 212, 800, 1195, 1346, 1398, 1405, 1474
BstSLI	GKGCMC	2 cut(s)	752, 1375
BstV1I	GCAGC	2 cut(s)	219, 873
BstV2I	GAAGAC	1 cut(s)	1428
BstX2I	RGATCY	1 cut(s)	448
BstYI	RGATCY	1 cut(s)	448
BstZI	CGGCCG	1 cut(s)	1082
BsuRI	GGCC	3 cut(s)	221, 1084, 1373
BtgI	CCRYGG	1 cut(s)	452
BtsCI	GGATG	3 cut(s)	471, 1285, 1337
BtsI	GCAGTG	1 cut(s)	648
BtsIMutI	CAGTG	3 cut(s)	84, 648, 1048
Cac8I	GCNNGC	2 cut(s)	219, 527
CaiI	CAGNNNCTG	1 cut(s)	383
CciI	TCATGA	1 cut(s)	876
Cfr10I	RCCGGY	1 cut(s)	529
Cfr13I	GGNCC	4 cut(s)	267, 1371, 1372, 1402
Csp6I	GTAC	6 cut(s)	100, 796, 1125, 1271, 1301, 1452
CviQI	GTAC	6 cut(s)	100, 796, 1125, 1271, 1301, 1452
DdeI	CTNAG	2 cut(s)	16, 91
DpnI	GATC	4 cut(s)	75, 255, 450, 678
DpnII	GATC	4 cut(s)	73, 253, 448, 676
DraI	TTTAAA	1 cut(s)	907
EaeI	YGGCCR	2 cut(s)	219, 1082
EagI	CGGCCG	1 cut(s)	1082
EclXI	CGGCCG	1 cut(s)	1082
Eco130I	CCWWGG	2 cut(s)	274, 452
Eco24I	GRGCYC	1 cut(s)	1375
Eco31I	GGTCTC	2 cut(s)	111, 1335
Eco47I	GGWCC	2 cut(s)	267, 1402
Eco52I	CGGCCG	1 cut(s)	1082
Eco57I	CTGAAG	1 cut(s)	1545
Eco91I	GGTNACC	1 cut(s)	632
EcoO109I	RGGNCCY	4 cut(s)	267, 1371, 1372, 1402
EcoO65I	GGTNACC	1 cut(s)	632
EcoRI	GAATTC	1 cut(s)	439
EcoRII	CCWGG	8 cut(s)	121, 212, 800, 1195, 1346, 1398, 1405, 1474
EcoT14I	CCWWGG	2 cut(s)	274, 452
EcoT38I	GRGCYC	1 cut(s)	1375
ErhI	CCWWGG	2 cut(s)	274, 452
FauI	CCCGC	1 cut(s)	331
FauNDI	CATATG	2 cut(s)	1225, 1232
FbaI	TGATCA	1 cut(s)	253
FblI	GTMKAC	1 cut(s)	600
Fnu4HI	GCNGC	2 cut(s)	208, 887
FokI	GGATG	3 cut(s)	478, 1272, 1344
FriOI	GRGCYC	1 cut(s)	1375
Fsp4HI	GCNGC	2 cut(s)	208, 887
FspBI	CTAG	5 cut(s)	275, 923, 1035, 1380, 1545
GluI	GCNGC	2 cut(s)	208, 887
GsuI	CTGGAG	1 cut(s)	1235
HaeIII	GGCC	3 cut(s)	221, 1084, 1373
HapII	CCGG	2 cut(s)	530, 1085
HincII	GTYRAC	1 cut(s)	50
HindII	GTYRAC	1 cut(s)	50
HindIII	AAGCTT	1 cut(s)	159
HinfI	GANTC	3 cut(s)	298, 317, 586
HpaII	CCGG	2 cut(s)	530, 1085
HphI	GGTGA	4 cut(s)	83, 353, 644, 1142
Hpy166II	GTNNAC	2 cut(s)	50, 601
Hpy188I	TCNGA	5 cut(s)	94, 322, 438, 448, 1023
Hpy188III	TCNNGA	8 cut(s)	515, 615, 680, 877, 1061, 1214, 1289, 1523
Hpy8I	GTNNAC	2 cut(s)	50, 601
HpyAV	CCTTC	1 cut(s)	1520
HpyCH4III	ACNGT	5 cut(s)	88, 411, 953, 1043, 1498
HpyCH4IV	ACGT	1 cut(s)	934
HpyCH4V	TGCA	7 cut(s)	64, 152, 174, 395, 824, 830, 1191
HpyF10VI	GCNNNNNNNGC	2 cut(s)	158, 227
HpyF3I	CTNAG	2 cut(s)	16, 91
HpySE526I	ACGT	1 cut(s)	934
Ksp22I	TGATCA	1 cut(s)	253
Kzo9I	GATC	4 cut(s)	73, 253, 448, 676
LmnI	GCTCC	2 cut(s)	950, 1357
Lsp1109I	GCAGC	2 cut(s)	219, 873
LweI	GCATC	3 cut(s)	673, 817, 882
MaeI	CTAG	5 cut(s)	275, 923, 1035, 1380, 1545
MaeII	ACGT	1 cut(s)	934
MaeIII	GTNAC	5 cut(s)	544, 632, 1030, 1117, 1235
MalI	GATC	4 cut(s)	75, 255, 450, 678
MboI	GATC	4 cut(s)	73, 253, 448, 676
MboII	GAAGA	8 cut(s)	142, 721, 780, 809, 1000, 1271, 1428, 1547
MflI	RGATCY	1 cut(s)	448
MhlI	GDGCHC	3 cut(s)	752, 1375, 1518
MmeI	TCCRAC	5 cut(s)	163, 740, 965, 972, 1318
MnlI	CCTC	7 cut(s)	280, 424, 650, 932, 935, 1116, 1385
MseI	TTAA	7 cut(s)	366, 477, 650, 843, 906, 930, 1443
MslI	CAYNNNNRTG	2 cut(s)	69, 861
MspCI	CTTAAG	1 cut(s)	476
MspI	CCGG	2 cut(s)	530, 1085
MspR9I	CCNGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
MvaI	CCWGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
MwoI	GCNNNNNNNGC	2 cut(s)	158, 227
NcoI	CCATGG	1 cut(s)	452
NdeI	CATATG	2 cut(s)	1225, 1232
NdeII	GATC	4 cut(s)	73, 253, 448, 676
NlaIV	GGNNCC	4 cut(s)	946, 1372, 1373, 1404
NmuCI	GTSAC	3 cut(s)	632, 1030, 1235
PagI	TCATGA	1 cut(s)	876
PfeI	GAWTC	3 cut(s)	298, 317, 586
PflMI	CCANNNNNTGG	3 cut(s)	569, 1010, 1353
PkrI	GCNGC	2 cut(s)	209, 888
PpuMI	RGGWCCY	2 cut(s)	267, 1402
PshBI	ATTAAT	1 cut(s)	1443
Psp5II	RGGWCCY	2 cut(s)	267, 1402
Psp6I	CCWGG	8 cut(s)	121, 212, 800, 1195, 1346, 1398, 1405, 1474
PspEI	GGTNACC	1 cut(s)	632
PspGI	CCWGG	8 cut(s)	121, 212, 800, 1195, 1346, 1398, 1405, 1474
PspN4I	GGNNCC	4 cut(s)	946, 1372, 1373, 1404
PspOMI	GGGCCC	1 cut(s)	1371
PspPI	GGNCC	4 cut(s)	267, 1371, 1372, 1402
PspPPI	RGGWCCY	2 cut(s)	267, 1402
PstNI	CAGNNNCTG	1 cut(s)	383
PsuI	RGATCY	1 cut(s)	448
RsaI	GTAC	6 cut(s)	101, 797, 1126, 1272, 1302, 1453
RsaNI	GTAC	6 cut(s)	100, 796, 1125, 1271, 1301, 1452
RseI	CAYNNNNRTG	2 cut(s)	69, 861
SaqAI	TTAA	7 cut(s)	366, 477, 650, 843, 906, 930, 1443
SatI	GCNGC	2 cut(s)	208, 887
Sau3AI	GATC	4 cut(s)	73, 253, 448, 676
Sau96I	GGNCC	4 cut(s)	267, 1371, 1372, 1402
ScrFI	CCNGG	8 cut(s)	123, 214, 802, 1197, 1348, 1400, 1407, 1476
SduI	GDGCHC	3 cut(s)	752, 1375, 1518
SfaNI	GCATC	3 cut(s)	673, 817, 882
SinI	GGWCC	2 cut(s)	267, 1402
SmiMI	CAYNNNNRTG	2 cut(s)	69, 861
SmlI	CTYRAG	2 cut(s)	476, 613
SmoI	CTYRAG	2 cut(s)	476, 613
SsiI	CCGC	2 cut(s)	338, 1487
SspMI	CTAG	5 cut(s)	275, 923, 1035, 1380, 1545
StyD4I	CCNGG	8 cut(s)	121, 212, 800, 1195, 1346, 1398, 1405, 1474
StyI	CCWWGG	2 cut(s)	274, 452
TaaI	ACNGT	5 cut(s)	88, 411, 953, 1043, 1498
TaiI	ACGT	1 cut(s)	937
TaqI	TCGA	2 cut(s)	1144, 1439
TaqII	GACCGA	1 cut(s)	651
TatI	WGTACW	2 cut(s)	99, 1300
TfiI	GAWTC	3 cut(s)	298, 317, 586
Tru1I	TTAA	7 cut(s)	366, 477, 650, 843, 906, 930, 1443
Tru9I	TTAA	7 cut(s)	366, 477, 650, 843, 906, 930, 1443
TscAI	CASTG	3 cut(s)	91, 648, 1048
TseFI	GTSAC	3 cut(s)	632, 1030, 1235
TseI	GCWGC	2 cut(s)	207, 886
Tsp45I	GTSAC	3 cut(s)	632, 1030, 1235
TspDTI	ATGAA	6 cut(s)	143, 192, 290, 330, 432, 599
TspGWI	ACGGA	3 cut(s)	498, 563, 1438
TspRI	CASTG	3 cut(s)	91, 648, 1048
Van91I	CCANNNNNTGG	3 cut(s)	569, 1010, 1353
Vha464I	CTTAAG	1 cut(s)	476
VpaK11BI	GGWCC	2 cut(s)	267, 1402
VspI	ATTAAT	1 cut(s)	1443
XapI	RAATTY	6 cut(s)	166, 439, 487, 556, 1208, 1275
XcmI	CCANNNNNNNNNTGG	2 cut(s)	459, 1290
XmaJI	CCTAGG	1 cut(s)	274
XmiI	GTMKAC	1 cut(s)	600
XspI	CTAG	5 cut(s)	275, 923, 1035, 1380, 1545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.