Prupe.2G136100_v2.0.a1

Necessary for protein translocation in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0011285
Physical Location & Seq
Reverse (-)
19376498 .. 19378377
1880 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G136100.1

Sequence Viewer

Length: 408 bp
ATGTTGATGGATGAGACAAAAGGAAACGACAACGCGTTTGCGTTACGTAATAAAACGGATTTTCAGCCCATCGGAGTCAAAAACCCTTCTCGGATCTTCAACCTTCGATACACGCCAGCATCTCAACTCGTTCTCTTCACCGAGTCGACTCAGAAAATGGTTCTAGGCGGTGGAGCACCTGCCCCAAGGGGAAGTGCCGCAGCTACTGCGAGCATGCGAAGGAGGAGGACGACAAGTGGAGGTGCTTCAGGCGGTGCAGCAGGGACAATGCTCCAGTTTTACACTGATGATGCCCCGGGACTCAAGATCTCCCCGAATGTTGTTCTCATCATGAGCATTGGTTTCATTGCCTTTGTTGCCGTTCTTCATGTCATGGGTAAGCTCTATTTTGTGCGCAGAGAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

136

Amino Acids

14.46

Weight (kDa)

10.47

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 187
Acc16I TGCGCA 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 146
AccII CGCG 1 cut(s) 35
AciI CCGC 3 cut(s) 168, 198, 252
AclWI GGATC 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 1 cut(s) 100
AluBI AGCT 2 cut(s) 203, 382
AluI AGCT 2 cut(s) 203, 382
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 1 cut(s) 101
AlwNI CAGNNNCTG 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 295
ApeKI GCWGC 2 cut(s) 200, 257
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AspLEI GCGC 1 cut(s) 396
AsuC2I CCSGG 2 cut(s) 296, 297
AsuHPI GGTGA 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 212, 269
BccI CCATC 1 cut(s) 77
BceAI ACGGC 1 cut(s) 344
BcnI CCSGG 2 cut(s) 296, 297
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 187
BglII AGATCT 1 cut(s) 306
BisI GCNGC 3 cut(s) 198, 201, 258
BlsI GCNGC 3 cut(s) 199, 202, 259
Bme1390I CCNGG 2 cut(s) 296, 297
BmeT110I CYCGRG 1 cut(s) 295
BmrFI CCNGG 2 cut(s) 296, 297
BmsI GCATC 2 cut(s) 128, 280
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 296, 297
BsaAI YACGTR 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 185, 294, 295
Bse1I ACTGG 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 185, 294, 295
BseGI GGATG 1 cut(s) 16
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 238
BseXI GCAGC 2 cut(s) 212, 269
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 295
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 2 cut(s) 277, 312
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 2 cut(s) 277, 312
BsoBI CYCGRG 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 178
Bsp143I GATC 2 cut(s) 93, 306
BspACI CCGC 3 cut(s) 168, 198, 252
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 3 cut(s) 185, 294, 295
BssMI GATC 2 cut(s) 93, 306
BssT1I CCWWGG 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 206
BstBAI YACGTR 1 cut(s) 47
BstC8I GCNNGC 3 cut(s) 117, 211, 215
BstDEI CTNAG 2 cut(s) 150, 405
BstF5I GGATG 1 cut(s) 16
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 2 cut(s) 96, 309
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 2 cut(s) 93, 306
BstMWI GCNNNNNNNGC 2 cut(s) 206, 356
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 294, 295
BstSNI TACGTA 1 cut(s) 47
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 212, 269
BstX2I RGATCY 2 cut(s) 93, 306
BstYI RGATCY 2 cut(s) 93, 306
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 3 cut(s) 117, 211, 215
CaiI CAGNNNCTG 1 cut(s) 206
CciI TCATGA 1 cut(s) 330
CfoI GCGC 1 cut(s) 396
Cfr9I CCCGGG 1 cut(s) 295
CviAII CATG 4 cut(s) 214, 331, 368, 373
CviJI RGCY 4 cut(s) 67, 203, 382, 404
CviKI_1 RGCY 4 cut(s) 67, 203, 382, 404
DdeI CTNAG 2 cut(s) 150, 405
DpnI GATC 2 cut(s) 95, 308
DpnII GATC 2 cut(s) 93, 306
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco105I TACGTA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 4 cut(s) 217, 334, 371, 376
FaiI YATR 4 cut(s) 215, 332, 369, 374
FaqI GGGAC 2 cut(s) 277, 312
FatI CATG 4 cut(s) 213, 330, 367, 372
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 198, 201, 258
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 3 cut(s) 198, 201, 258
FspBI CTAG 1 cut(s) 164
FspI TGCGCA 1 cut(s) 395
GlaI GCGC 1 cut(s) 395
GluI GCNGC 3 cut(s) 198, 201, 258
GsuI CTGGAG 1 cut(s) 257
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 396
Hin1II CATG 4 cut(s) 217, 334, 371, 376
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 4 cut(s) 75, 143, 148, 300
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 3 cut(s) 74, 93, 153
Hpy188III TCNNGA 2 cut(s) 304, 331
Hpy8I GTNNAC 1 cut(s) 147
HpyAV CCTTC 3 cut(s) 96, 113, 213
HpyCH4IV ACGT 1 cut(s) 46
HpyCH4V TGCA 1 cut(s) 257
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 356
HpyF3I CTNAG 2 cut(s) 150, 405
HpySE526I ACGT 1 cut(s) 46
Hsp92II CATG 4 cut(s) 217, 334, 371, 376
HspAI GCGC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 93, 306
LmnI GCTCC 2 cut(s) 173, 276
LpnPI CCDG 6 cut(s) 129, 192, 234, 246, 287, 309
Lsp1109I GCAGC 2 cut(s) 212, 269
LweI GCATC 2 cut(s) 128, 280
MaeI CTAG 1 cut(s) 164
MaeII ACGT 1 cut(s) 46
MaeIII GTNAC 1 cut(s) 42
MalI GATC 2 cut(s) 95, 308
MboI GATC 2 cut(s) 93, 306
MboII GAAGA 3 cut(s) 88, 127, 356
MflI RGATCY 2 cut(s) 93, 306
MhlI GDGCHC 1 cut(s) 178
MluI ACGCGT 1 cut(s) 33
MlyI GAGTC 4 cut(s) 84, 142, 152, 294
MnlI CCTC 4 cut(s) 216, 219, 233, 394
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 296, 297
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 206, 356
NciI CCSGG 2 cut(s) 296, 297
NdeII GATC 2 cut(s) 93, 306
NlaIII CATG 4 cut(s) 217, 334, 371, 376
NsbI TGCGCA 1 cut(s) 395
NspI RCATGY 1 cut(s) 217
PaeI GCATGC 1 cut(s) 217
PagI TCATGA 1 cut(s) 330
PaqCI CACCTGC 1 cut(s) 187
PkrI GCNGC 3 cut(s) 199, 202, 259
PleI GAGTC 4 cut(s) 83, 142, 151, 294
PpsI GAGTC 4 cut(s) 83, 142, 151, 294
Ppu21I YACGTR 1 cut(s) 47
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 2 cut(s) 93, 306
SalI GTCGAC 1 cut(s) 145
SatI GCNGC 3 cut(s) 198, 201, 258
Sau3AI GATC 2 cut(s) 93, 306
SchI GAGTC 4 cut(s) 84, 142, 152, 294
ScrFI CCNGG 2 cut(s) 296, 297
SduI GDGCHC 1 cut(s) 178
SetI ASST 6 cut(s) 49, 105, 181, 205, 244, 384
SfaNI GCATC 2 cut(s) 128, 280
SmaI CCCGGG 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SnaBI TACGTA 1 cut(s) 47
SphI GCATGC 1 cut(s) 217
SsiI CCGC 3 cut(s) 168, 198, 252
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 294, 295
StyI CCWWGG 1 cut(s) 185
TaiI ACGT 1 cut(s) 49
TaqI TCGA 2 cut(s) 106, 146
TauI GCSGC 1 cut(s) 200
TscAI CASTG 1 cut(s) 289
TseI GCWGC 2 cut(s) 200, 257
TspDTI ATGAA 2 cut(s) 334, 356
TspGWI ACGGA 1 cut(s) 71
TspMI CCCGGG 1 cut(s) 295
TspRI CASTG 1 cut(s) 289
XceI RCATGY 1 cut(s) 217
XmaI CCCGGG 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 146
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.