Rorug01G0197600

posttranslational protein targeting to membrane, translocation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011285
Physical Location & Seq
Forward (+)
27672620 .. 27673788
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0197600.1

Sequence Viewer

Length: 795 bp
ATGGCACTCCTTCCAATTCAAAATGCTCTACTTCCCAATCTCAAACCCTCTCCCTTCCTCACACCCAAAAACCCATTCCCCCTCCTCACCAAAACCCACAAGAAGCCCCACTCCACAGTCACCATGATGAGCATGGAGGCCGGCATCGGCGTCATGGCCACCAAGCTCGGCATGATGAGCTTCTTCGAACCCAACGGCAAGGTCGTCCCCGTCACCGTCGTCGGCTTCAAAGAAGGCAACATTGTCACCCAAATCAAAACCCAAGCCACCGACGGCTACGACGCCGTCCAAGTCGGCTACCGCCGCATCCGCGACCGGAAACTCACCAAGCCCGAAATGGGCCATCTCAACAAGGCCGGCGCAATCCCTCTCCGCCACCTCCAGGAGTTTCGTCTTCAGGACATTGAAGGGTTTGAGCCCAACCAGAAGCTTGATTTCAGCGAGCTCTTCAAGGAGGGCGACCTCGTCGACGTCGCCGGAACCACGATCGGGAAAGGGTTTCAGGGTGGGATTAAGAGGCACCATTTCAAGAGGGGGCTGATGACTCATGGGTCCAAGAGTCACAGACAGCTTGGGTCGATTGGGGCCGGAACCACTCCCGGGAGGGTTTACAAGGGGAAGAAGATGCCTGGGAGAATGGGAGGGACTAAGACCAAGATCAGGAAGCTCAAGATTGTCAAGATTGATAAGGACCTCAATGTGGTTATGATTAATGGTGCTCTTCCTGGTAAGCCTGGGAACCTTCTCCGCATTACTCCGGCTAAGATTGTAGGGAAGAATATCCCCAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0003674 GO:0005085 GO:0005086 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005784 GO:0005789 GO:0005791 GO:0005829 GO:0006508 GO:0006511 GO:0006605 GO:0006606 GO:0006612 GO:0006613 GO:0006614 GO:0006616 GO:0006620 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006996 GO:0007029 GO:0008104 GO:0008150 GO:0008152 GO:0008320 GO:0008565 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010243 GO:0010256 GO:0010498 GO:0010506 GO:0010507 GO:0012505 GO:0015031 GO:0015399 GO:0015405 GO:0015450 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019222 GO:0019538 GO:0019838 GO:0019899 GO:0019941 GO:0022804 GO:0022857 GO:0022884 GO:0030163 GO:0030433 GO:0030867 GO:0030970 GO:0031090 GO:0031204 GO:0031205 GO:0031224 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031984 GO:0032527 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034976 GO:0036503 GO:0042175 GO:0042221 GO:0042562 GO:0042886 GO:0042887 GO:0043021 GO:0043022 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045047 GO:0045048 GO:0045184 GO:0046907 GO:0048408 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051169 GO:0051170 GO:0051179 GO:0051205 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0055085 GO:0061024 GO:0065002 GO:0065007 GO:0065009 GO:0070727 GO:0070972 GO:0071256 GO:0071261 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071816 GO:0071840 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0098588 GO:0098772 GO:0098796 GO:0098827 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1903513 GO:1904680
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

264

Amino Acids

28.9

Weight (kDa)

10.42

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 143 - 230 7.8e-16 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 550
AatII GACGTC 1 cut(s) 474
AccB1I GGYRCC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 312
AciI CCGC 5 cut(s) 301, 304, 310, 373, 748
AcoI YGGCCR 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 380
AcyI GRCGYC 3 cut(s) 150, 282, 471
AfiI CCNNNNNNNGG 6 cut(s) 338, 382, 489, 600, 660, 700
AgsI TTSAA 5 cut(s) 20, 229, 407, 451, 529
AjnI CCWGG 4 cut(s) 381, 628, 724, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 6 cut(s) 166, 180, 430, 445, 571, 667
AluI AGCT 6 cut(s) 166, 180, 430, 445, 571, 667
Alw21I GWGCWC 2 cut(s) 447, 721
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 5 cut(s) 138, 156, 340, 354, 585
AseI ATTAAT 1 cut(s) 711
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 4 cut(s) 340, 552, 585, 691
AsuC2I CCSGG 2 cut(s) 600, 601
AsuHPI GGTGA 5 cut(s) 79, 112, 205, 238, 316
AsuII TTCGAA 1 cut(s) 186
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 2 cut(s) 552, 691
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 519
BanII GRGCYC 2 cut(s) 420, 447
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 2 cut(s) 447, 721
BccI CCATC 1 cut(s) 351
BceAI ACGGC 3 cut(s) 211, 269, 289
BciT130I CCWGG 4 cut(s) 383, 630, 726, 735
BcnI CCSGG 2 cut(s) 600, 601
BisI GCNGC 1 cut(s) 304
BlsI GCNGC 1 cut(s) 305
Bme1390I CCNGG 6 cut(s) 383, 600, 601, 630, 726, 735
Bme18I GGWCC 2 cut(s) 552, 691
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 4 cut(s) 340, 552, 585, 691
BmiI GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
BmrFI CCNGG 6 cut(s) 383, 600, 601, 630, 726, 735
BmsI GCATC 3 cut(s) 153, 315, 615
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 600, 601
BsaHI GRCGYC 3 cut(s) 150, 282, 471
BsaJI CCNNGG 3 cut(s) 599, 629, 734
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 6 cut(s) 338, 382, 489, 600, 660, 700
Bse118I RCCGGY 2 cut(s) 140, 356
BseBI CCWGG 4 cut(s) 383, 630, 726, 735
BseDI CCNNGG 3 cut(s) 599, 629, 734
BseGI GGATG 1 cut(s) 306
BseLI CCNNNNNNNGG 6 cut(s) 338, 382, 489, 600, 660, 700
BseRI GAGGAG 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 2 cut(s) 316, 489
BshFI GGCC 5 cut(s) 140, 158, 342, 356, 587
BshNI GGYRCC 1 cut(s) 519
BsiEI CGRYCG 2 cut(s) 316, 489
BsiHKAI GWGCWC 2 cut(s) 447, 721
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 7 cut(s) 141, 316, 357, 477, 588, 600, 758
BslFI GGGAC 2 cut(s) 191, 658
BslI CCNNNNNNNGG 6 cut(s) 338, 382, 489, 600, 660, 700
BsmFI GGGAC 2 cut(s) 191, 658
BsnI GGCC 5 cut(s) 140, 158, 342, 356, 587
BsoBI CYCGRG 1 cut(s) 599
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 3 cut(s) 420, 447, 721
Bsp143I GATC 2 cut(s) 486, 657
BspACI CCGC 5 cut(s) 301, 304, 310, 373, 748
BspANI GGCC 5 cut(s) 140, 158, 342, 356, 587
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
BspQI GCTCTTC 2 cut(s) 452, 726
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 140, 356
BssAI RCCGGY 2 cut(s) 140, 356
BssECI CCNNGG 3 cut(s) 599, 629, 734
BssMI GATC 2 cut(s) 486, 657
BssNI GRCGYC 3 cut(s) 150, 282, 471
Bst2UI CCWGG 4 cut(s) 383, 630, 726, 735
Bst4CI ACNGT 2 cut(s) 118, 217
Bst6I CTCTTC 2 cut(s) 452, 726
BstACI GRCGYC 3 cut(s) 150, 282, 471
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 3 cut(s) 142, 358, 443
BstDEI CTNAG 2 cut(s) 648, 762
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 2 cut(s) 489, 660
BstMBI GATC 2 cut(s) 486, 657
BstMCI CGRYCG 2 cut(s) 316, 489
BstMWI GCNNNNNNNGC 3 cut(s) 177, 303, 309
BstNI CCWGG 4 cut(s) 383, 630, 726, 735
BstSCI CCNGG 6 cut(s) 381, 598, 599, 628, 724, 733
BstUI CGCG 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 386
BsuRI GGCC 5 cut(s) 140, 158, 342, 356, 587
BtsCI GGATG 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 142, 358, 443
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 2 cut(s) 140, 356
Cfr13I GGNCC 4 cut(s) 340, 552, 585, 691
Cfr9I CCCGGG 1 cut(s) 599
CseI GACGC 2 cut(s) 139, 290
CviAII CATG 5 cut(s) 124, 133, 154, 172, 548
DdeI CTNAG 2 cut(s) 648, 762
DpnI GATC 2 cut(s) 488, 659
DpnII GATC 2 cut(s) 486, 657
DrdI GACNNNNNNGTC 1 cut(s) 550
DseDI GACNNNNNNGTC 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 452, 726
EarI CTCTTC 2 cut(s) 452, 726
EciI GGCGGA 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 2 cut(s) 420, 447
Eco47I GGWCC 2 cut(s) 552, 691
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 599
EcoICRI GAGCTC 1 cut(s) 445
EcoO109I RGGNCCY 1 cut(s) 691
EcoRII CCWGG 4 cut(s) 381, 628, 724, 733
EcoT38I GRGCYC 2 cut(s) 420, 447
FaeI CATG 5 cut(s) 127, 136, 157, 175, 551
FaiI YATR 6 cut(s) 125, 134, 155, 173, 549, 707
FaqI GGGAC 2 cut(s) 191, 658
FatI CATG 5 cut(s) 123, 132, 153, 171, 547
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 304
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 2 cut(s) 420, 447
Fsp4HI GCNGC 1 cut(s) 304
GlaI GCGC 1 cut(s) 361
GluI GCNGC 1 cut(s) 304
GsuI CTGGAG 1 cut(s) 365
HaeIII GGCC 5 cut(s) 140, 158, 342, 356, 587
HapII CCGG 7 cut(s) 141, 316, 357, 477, 588, 600, 758
HgaI GACGC 2 cut(s) 139, 290
HhaI GCGC 1 cut(s) 362
Hin1I GRCGYC 3 cut(s) 150, 282, 471
Hin1II CATG 5 cut(s) 127, 136, 157, 175, 551
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 2 cut(s) 544, 559
HpaII CCGG 7 cut(s) 141, 316, 357, 477, 588, 600, 758
HphI GGTGA 5 cut(s) 79, 112, 205, 238, 316
Hpy166II GTNNAC 2 cut(s) 469, 610
Hpy188III TCNNGA 6 cut(s) 398, 490, 529, 661, 670, 679
Hpy8I GTNNAC 2 cut(s) 469, 610
Hpy99I CGWCG 7 cut(s) 221, 224, 275, 284, 470, 473, 476
HpyAV CCTTC 5 cut(s) 20, 64, 227, 401, 752
HpyCH4III ACNGT 2 cut(s) 118, 217
HpyCH4IV ACGT 1 cut(s) 471
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 303, 309
HpyF3I CTNAG 2 cut(s) 648, 762
HpySE526I ACGT 1 cut(s) 471
Hsp92I GRCGYC 3 cut(s) 150, 282, 471
Hsp92II CATG 5 cut(s) 127, 136, 157, 175, 551
HspAI GCGC 1 cut(s) 360
KroI GCCGGC 2 cut(s) 140, 356
KroNI GCCGGC 2 cut(s) 142, 358
Kzo9I GATC 2 cut(s) 486, 657
LguI GCTCTTC 2 cut(s) 452, 726
LweI GCATC 3 cut(s) 153, 315, 615
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 4 cut(s) 118, 211, 244, 560
MalI GATC 2 cut(s) 488, 659
MboI GATC 2 cut(s) 486, 657
MboII GAAGA 7 cut(s) 175, 386, 439, 631, 634, 713, 787
MhlI GDGCHC 3 cut(s) 420, 447, 721
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 2 cut(s) 538, 568
Mox20I TGGCCA 1 cut(s) 158
MroNI GCCGGC 2 cut(s) 140, 356
MscI TGGCCA 1 cut(s) 158
MseI TTAA 3 cut(s) 513, 711, 793
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 7 cut(s) 141, 316, 357, 477, 588, 600, 758
MspR9I CCNGG 6 cut(s) 383, 600, 601, 630, 726, 735
MvaI CCWGG 4 cut(s) 383, 630, 726, 735
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 177, 303, 309
NaeI GCCGGC 2 cut(s) 142, 358
NciI CCSGG 2 cut(s) 600, 601
NdeII GATC 2 cut(s) 486, 657
NgoMIV GCCGGC 2 cut(s) 140, 356
NlaIII CATG 5 cut(s) 127, 136, 157, 175, 551
NlaIV GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 4 cut(s) 118, 211, 244, 560
NspV TTCGAA 1 cut(s) 186
PciSI GCTCTTC 2 cut(s) 452, 726
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 2 cut(s) 142, 358
PflFI GACNNNGTC 2 cut(s) 284, 464
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 1 cut(s) 305
Ple19I CGATCG 1 cut(s) 489
PleI GAGTC 2 cut(s) 538, 567
PpsI GAGTC 2 cut(s) 538, 567
PpuMI RGGWCCY 1 cut(s) 691
PshBI ATTAAT 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 447
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 4 cut(s) 381, 628, 724, 733
PspGI CCWGG 4 cut(s) 381, 628, 724, 733
PspN4I GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
PspPI GGNCC 4 cut(s) 340, 552, 585, 691
PspPPI RGGWCCY 1 cut(s) 691
PsyI GACNNNGTC 2 cut(s) 284, 464
PvuI CGATCG 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 125
SacI GAGCTC 1 cut(s) 447
SalI GTCGAC 1 cut(s) 467
SapI GCTCTTC 2 cut(s) 452, 726
SaqAI TTAA 3 cut(s) 513, 711, 793
SatI GCNGC 1 cut(s) 304
Sau3AI GATC 2 cut(s) 486, 657
Sau96I GGNCC 4 cut(s) 340, 552, 585, 691
SchI GAGTC 2 cut(s) 538, 568
ScrFI CCNGG 6 cut(s) 383, 600, 601, 630, 726, 735
SduI GDGCHC 3 cut(s) 420, 447, 721
SfaNI GCATC 3 cut(s) 153, 315, 615
SfuI TTCGAA 1 cut(s) 186
SgrDI CGTCGACG 1 cut(s) 467
SinI GGWCC 2 cut(s) 552, 691
SmaI CCCGGG 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 1 cut(s) 15
SsiI CCGC 5 cut(s) 301, 304, 310, 373, 748
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 6 cut(s) 381, 598, 599, 628, 724, 733
TaaI ACNGT 2 cut(s) 118, 217
TaiI ACGT 1 cut(s) 474
TaqI TCGA 3 cut(s) 186, 468, 578
TasI AATT 1 cut(s) 15
TauI GCSGC 1 cut(s) 306
Tru1I TTAA 3 cut(s) 513, 711, 793
Tru9I TTAA 3 cut(s) 513, 711, 793
TseFI GTSAC 4 cut(s) 118, 211, 244, 560
Tsp45I GTSAC 4 cut(s) 118, 211, 244, 560
TspMI CCCGGG 1 cut(s) 599
Tth111I GACNNNGTC 2 cut(s) 284, 464
VpaK11BI GGWCC 2 cut(s) 552, 691
VspI ATTAAT 1 cut(s) 711
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 334
XmaI CCCGGG 1 cut(s) 599
XmiI GTMKAC 1 cut(s) 468
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.