Prupe.2G181200_v2.0.a1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0017746
Physical Location & Seq
Forward (+)
22420745 .. 22424333
3589 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G181200.4

Sequence Viewer

Length: 969 bp
ATGCCAAGCTCTTCTAAATCCCGAGTGATATTATCGATTGGCAATCATCATGACCAACCTGATCATTTCAGGAAGGATCGCTTCATGGATGATGCTAATGGTTCATGCAGAGGGAAGAATTTAGAATGTGTATCAGGTTGTAGTGAAAGGAATTTGAGGAGAAAATTGGATGACGAGGATGATGGGACCAATTGTTCGCAAGATGCTTGTTATGCAGGTAGCCAAAGTAAGGCACAAATTACAGCCAGCAAGTGTGTGGAAGTTGAGGTTTCAACAGGCTCAAGTTCAAGTTCAAGTTTAGTAGATATTTCTTTAGGTGTTCATGAGGATGCCCCTCTGTCTACCCAAGGAAGACTAGAGAGCAGAGATGATGTTCATGATGTTGAGAATATAAGTACTATCTCAGGGAGTAATCTCAGGGAAGGTGGCTGTGTAGAGAGAGTAAGCAACAGCCTATCATCAGATAGGACAAATAAGGGAAGTTACACGAGTACAAAACTAACACAAGCATCTAAGATGCAATGGCCAAAGTGGACAGTGTCTCACATGTTGAGTTTGTCGAGCATGGAGAAAGTGATGAATTATTACCGTGTTTTGAATTTGGCCGATAACTTGAATGATGAGGATCTACGTTGTTTCTTGGAGGAACAGCTTTCCAAGGAGAAAGCTGGGTTGTCAGAGACGACCATTACGAACCGTTTAAGAACAAGACTCCATGTATCGGAAACAGTGCGGGAACCCGATAGTTGCAGCATTTGCATGATTGAATATAAGGACCAAGACAAGATTGCGAGTTTATATTACTGCTCTCATGAATATCATTCCGACTGCATAAAGGAGTGGCTTCTGAAGAACAACCTTTGCCCGATGTGTAGGGCACTAGCTATAATCCCAGAAGACTACCCCTGTTGGGATGGTGATGTGCGGTTACACCAAGCATTCTTTGTGCATTTGAAGCTTGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

323

Amino Acids

36.14

Weight (kDa)

5.59

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 341
AciI CCGC 2 cut(s) 733, 925
AclWI GGATC 2 cut(s) 84, 633
AcoI YGGCCR 2 cut(s) 524, 603
AcsI RAATTY 3 cut(s) 118, 151, 598
AcuI CTGAAG 1 cut(s) 869
AfaI GTAC 2 cut(s) 397, 493
AfiI CCNNNNNNNGG 3 cut(s) 229, 721, 910
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 7 cut(s) 273, 288, 294, 598, 616, 767, 955
AloI GAACNNNNNNTCC 2 cut(s) 178, 210
AluBI AGCT 6 cut(s) 9, 652, 668, 884, 958, 965
AluI AGCT 6 cut(s) 9, 652, 668, 884, 958, 965
Alw26I GTCTC 2 cut(s) 546, 674
AlwI GGATC 2 cut(s) 84, 633
Ama87I CYCGRG 1 cut(s) 21
AoxI GGCC 2 cut(s) 524, 603
ApeKI GCWGC 2 cut(s) 750, 962
ApoI RAATTY 3 cut(s) 118, 151, 598
ArsI GACNNNNNNTTYG 2 cut(s) 178, 210
AspS9I GGNCC 2 cut(s) 186, 775
AsuHPI GGTGA 1 cut(s) 929
AvaI CYCGRG 1 cut(s) 21
AvaII GGWCC 2 cut(s) 186, 775
BaeGI GKGCMC 1 cut(s) 880
BalI TGGCCA 1 cut(s) 526
BauI CACGAG 1 cut(s) 487
BbsI GAAGAC 2 cut(s) 358, 903
BbvI GCAGC 1 cut(s) 762
BccI CCATC 2 cut(s) 176, 908
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 546, 674
BfaI CTAG 2 cut(s) 356, 881
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 2 cut(s) 751, 963
BlsI GCNGC 2 cut(s) 752, 964
BmcAI AGTACT 1 cut(s) 397
Bme18I GGWCC 2 cut(s) 186, 775
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 186, 775
BmiI GGNNCC 2 cut(s) 187, 738
BmsI GCATC 5 cut(s) 82, 193, 319, 507, 518
BpiI GAAGAC 2 cut(s) 358, 903
BpuEI CTTGAG 1 cut(s) 265
Bsa29I ATCGAT 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 624
BsaJI CCNNGG 2 cut(s) 346, 657
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 721, 910
Bse3DI GCAATG 1 cut(s) 527
Bse8I GATNNNNATC 1 cut(s) 624
BseCI ATCGAT 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 346, 657
BseGI GGATG 5 cut(s) 94, 175, 184, 334, 919
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 3 cut(s) 229, 721, 910
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 417, 430
BseRI GAGGAG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 1 cut(s) 762
BseYI CCCAGC 1 cut(s) 668
BshFI GGCC 2 cut(s) 526, 605
BshVI ATCGAT 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 3 cut(s) 229, 721, 910
BsmAI GTCTC 2 cut(s) 546, 674
BsmBI CGTCTC 1 cut(s) 674
BsmFI GGGAC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 2 cut(s) 526, 605
BsoBI CYCGRG 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 880
Bsp143I GATC 3 cut(s) 61, 76, 625
BspACI CCGC 2 cut(s) 733, 925
BspANI GGCC 2 cut(s) 526, 605
BspCNI CTCAG 2 cut(s) 416, 429
BspDI ATCGAT 1 cut(s) 35
BspHI TCATGA 4 cut(s) 49, 322, 376, 811
BspLI GGNNCC 2 cut(s) 187, 738
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 84, 633
BspQI GCTCTTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 527
BssECI CCNNGG 2 cut(s) 346, 657
BssMI GATC 3 cut(s) 61, 76, 625
BssSI CACGAG 1 cut(s) 487
BssT1I CCWWGG 2 cut(s) 346, 657
Bst2BI CACGAG 1 cut(s) 487
Bst4CI ACNGT 4 cut(s) 538, 590, 698, 730
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 756
BstC8I GCNNGC 2 cut(s) 247, 960
BstDEI CTNAG 3 cut(s) 403, 416, 513
BstF5I GGATG 5 cut(s) 94, 175, 184, 334, 919
BstKTI GATC 3 cut(s) 64, 79, 628
BstMAI GTCTC 2 cut(s) 546, 674
BstMBI GATC 3 cut(s) 61, 76, 625
BstMWI GCNNNNNNNGC 3 cut(s) 212, 756, 955
BstNSI RCATGY 1 cut(s) 550
BstSLI GKGCMC 1 cut(s) 880
BstV1I GCAGC 1 cut(s) 762
BstV2I GAAGAC 2 cut(s) 358, 903
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
Bsu15I ATCGAT 1 cut(s) 35
BsuRI GGCC 2 cut(s) 526, 605
BsuTUI ATCGAT 1 cut(s) 35
BtsCI GGATG 5 cut(s) 94, 175, 184, 334, 919
BtsIMutI CAGTG 2 cut(s) 543, 735
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 247, 960
CciI TCATGA 4 cut(s) 49, 322, 376, 811
Cfr13I GGNCC 2 cut(s) 186, 775
ClaI ATCGAT 1 cut(s) 35
Csp6I GTAC 2 cut(s) 396, 492
CviQI GTAC 2 cut(s) 396, 492
DdeI CTNAG 3 cut(s) 403, 416, 513
DpnI GATC 3 cut(s) 63, 78, 627
DpnII GATC 3 cut(s) 61, 76, 625
EaeI YGGCCR 2 cut(s) 524, 603
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 2 cut(s) 346, 657
Eco47I GGWCC 2 cut(s) 186, 775
Eco57I CTGAAG 1 cut(s) 869
Eco88I CYCGRG 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 346, 657
ErhI CCWWGG 2 cut(s) 346, 657
Esp3I CGTCTC 1 cut(s) 674
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 199
FauI CCCGC 1 cut(s) 726
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 2 cut(s) 751, 963
FokI GGATG 5 cut(s) 101, 182, 191, 341, 926
Fsp4HI GCNGC 2 cut(s) 751, 963
FspBI CTAG 2 cut(s) 356, 881
GluI GCNGC 2 cut(s) 751, 963
GsaI CCCAGC 1 cut(s) 672
HaeIII GGCC 2 cut(s) 526, 605
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 1 cut(s) 711
HphI GGTGA 1 cut(s) 929
Hpy166II GTNNAC 2 cut(s) 342, 534
Hpy188I TCNGA 5 cut(s) 463, 679, 724, 826, 849
Hpy188III TCNNGA 6 cut(s) 21, 50, 70, 323, 377, 812
Hpy8I GTNNAC 2 cut(s) 342, 534
HpyAV CCTTC 2 cut(s) 67, 416
HpyCH4III ACNGT 4 cut(s) 538, 590, 698, 730
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 8 cut(s) 108, 215, 520, 750, 759, 831, 949, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 756, 955
HpyF3I CTNAG 3 cut(s) 403, 416, 513
HpySE526I ACGT 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 3 cut(s) 61, 76, 625
LguI GCTCTTC 1 cut(s) 16
Lsp1109I GCAGC 1 cut(s) 762
LweI GCATC 5 cut(s) 82, 193, 319, 507, 518
MaeI CTAG 2 cut(s) 356, 881
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 2 cut(s) 482, 927
MalI GATC 3 cut(s) 63, 78, 627
MboI GATC 3 cut(s) 61, 76, 625
MboII GAAGA 5 cut(s) 3, 127, 363, 862, 908
MfeI CAATTG 1 cut(s) 190
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 880
MlsI TGGCCA 1 cut(s) 526
MluCI AATT 7 cut(s) 118, 151, 164, 190, 237, 580, 598
MluNI TGGCCA 1 cut(s) 526
MlyI GAGTC 1 cut(s) 705
MmeI TCCRAC 1 cut(s) 849
MnlI CCTC 8 cut(s) 104, 150, 169, 259, 319, 345, 616, 637
Mox20I TGGCCA 1 cut(s) 526
MscI TGGCCA 1 cut(s) 526
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 2 cut(s) 327, 758
Msp20I TGGCCA 1 cut(s) 526
MunI CAATTG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 938
MwoI GCNNNNNNNGC 3 cut(s) 212, 756, 955
NdeII GATC 3 cut(s) 61, 76, 625
NlaIV GGNNCC 2 cut(s) 187, 738
NspI RCATGY 1 cut(s) 550
PagI TCATGA 4 cut(s) 49, 322, 376, 811
PciI ACATGT 1 cut(s) 546
PciSI GCTCTTC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 689
PctI GAATGC 1 cut(s) 938
PflFI GACNNNGTC 1 cut(s) 538
PkrI GCNGC 2 cut(s) 752, 964
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
PscI ACATGT 1 cut(s) 546
PspFI CCCAGC 1 cut(s) 668
PspN4I GGNNCC 2 cut(s) 187, 738
PspPI GGNCC 2 cut(s) 186, 775
PsuI RGATCY 1 cut(s) 625
PsyI GACNNNGTC 1 cut(s) 538
RsaI GTAC 2 cut(s) 397, 493
RsaNI GTAC 2 cut(s) 396, 492
RseI CAYNNNNRTG 2 cut(s) 327, 758
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 2 cut(s) 751, 963
Sau3AI GATC 3 cut(s) 61, 76, 625
Sau96I GGNCC 2 cut(s) 186, 775
ScaI AGTACT 1 cut(s) 397
SchI GAGTC 1 cut(s) 705
SduI GDGCHC 1 cut(s) 880
SfaNI GCATC 5 cut(s) 82, 193, 319, 507, 518
SinI GGWCC 2 cut(s) 186, 775
SmiMI CAYNNNNRTG 2 cut(s) 327, 758
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 7 cut(s) 118, 151, 164, 190, 237, 580, 598
SsiI CCGC 2 cut(s) 733, 925
SspMI CTAG 2 cut(s) 356, 881
StyI CCWWGG 2 cut(s) 346, 657
TaaI ACNGT 4 cut(s) 538, 590, 698, 730
TaiI ACGT 1 cut(s) 634
TaqI TCGA 2 cut(s) 35, 560
TasI AATT 7 cut(s) 118, 151, 164, 190, 237, 580, 598
TatI WGTACW 2 cut(s) 395, 491
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 2 cut(s) 543, 735
TseI GCWGC 2 cut(s) 750, 962
TspDTI ATGAA 6 cut(s) 73, 93, 311, 365, 593, 828
TspRI CASTG 2 cut(s) 543, 735
Tth111I GACNNNGTC 1 cut(s) 538
VpaK11BI GGWCC 2 cut(s) 186, 775
XapI RAATTY 3 cut(s) 118, 151, 598
XceI RCATGY 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 341
XspI CTAG 2 cut(s) 356, 881
ZrmI AGTACT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.