Rh1DG089500

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017746
Physical Location & Seq
Reverse (-)
16700932 .. 16715811
14880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG089500.1

Sequence Viewer

Length: 927 bp
ATGGCAGATCCTAACAAATCCCACTTAGTACCATCGGTTTTCATTGATCACGATGAAAATGGCCATGGCGGCAACGGTAACTTCATGGATGAAGTTGATGGTTCAAGTAATATAGACAATTACGAGTGCAGTGCGCAAGATTATGGTTATACGACAGGTGGCCAAATTAATACACAAATTACAACAAGCAAATTTCGAGCTGCTGATATTTCTATAGGCTCAAATTCAGGGAAGGTCGACTCTGAGGACGAGCTTACTGAATCTTCCATCTATGATTATATTCAAGTACACGTCCAAGGGATCTTTCCACAGCATCGCGTTATAGTCGACTGTGGTGAATCGCTAAGATCTCATATGGCTTCATCAACAGAACGCCGCAGTCCATGGCAGATTAATTTAATTGATCTGTTTCCACCCCACTATTATAACTCGGTGGTGTACTGGAGGACAAAAGTAGCTGAAGCTTCCAAGATCCAAAGACCAATGTGGACGGTGTCACACATGATGACTTTGTCACCCATGGAGAAAGTTATTAGTTACTACCGTGCTTGGAAGGTGGCTGACAACTTGAATGATGAGGATTTGCGTTGTTTCTTGGAGGAACAGCTGTGCAAGGAGAAAGCAGGGCTGTCAGAGAAAACTATTGCAAGGCGTTTAAAAACAAAGGTTTATGTAGCATCGAAAGTGGAGGAAAACCCCGATGCTTGTAGTATTTGCATGGCCGGATACAAGGACCGTGACAAGGTTGCGAGTTTATACTATTGTTGTCATGAGTATCACGAAGAGTGCATAAAGGAGTGGCTTCTCAAGAGCAACCTCTGCCCCATGTGTAGAGCATTAGCTATAATCCCGGAAGATTACCCCTGTTGGGAAGCTGATGTGCAGCTACACCAAGCATTCTTTCTGCATTTACAGTACGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

308

Amino Acids

35.24

Weight (kDa)

5.29

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 235 - 278 1.7e-11 Ring finger domain
zf-rbx1 PF12678 235 - 278 4.6e-07 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 236 - 277 3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
Acc16I TGCGCA 1 cut(s) 135
AccI GTMKAC 2 cut(s) 237, 327
AccII CGCG 1 cut(s) 318
AciI CCGC 2 cut(s) 69, 376
AclWI GGATC 3 cut(s) 2, 308, 466
AcoI YGGCCR 3 cut(s) 61, 160, 720
AcsI RAATTY 2 cut(s) 191, 223
AcuI CTGAAG 1 cut(s) 480
AfaI GTAC 4 cut(s) 30, 288, 440, 917
AfiI CCNNNNNNNGG 2 cut(s) 742, 868
AflIII ACRYGT 1 cut(s) 289
AgsI TTSAA 3 cut(s) 105, 284, 571
AjiI CACGTC 1 cut(s) 292
AjuI GAANNNNNNNTTGG 4 cut(s) 288, 320, 885, 917
AluBI AGCT 8 cut(s) 200, 253, 458, 464, 607, 842, 875, 886
AluI AGCT 8 cut(s) 200, 253, 458, 464, 607, 842, 875, 886
AlwI GGATC 3 cut(s) 2, 308, 466
AoxI GGCC 3 cut(s) 61, 160, 720
ApeKI GCWGC 2 cut(s) 200, 883
ApoI RAATTY 2 cut(s) 191, 223
AseI ATTAAT 2 cut(s) 168, 393
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 733
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 347, 507
AvaII GGWCC 1 cut(s) 733
BalI TGGCCA 2 cut(s) 63, 162
BbvI GCAGC 2 cut(s) 187, 895
BccI CCATC 3 cut(s) 40, 92, 275
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciVI GTATCC 1 cut(s) 719
BclI TGATCA 1 cut(s) 46
BcnI CCSGG 1 cut(s) 851
BfmI CTRYAG 1 cut(s) 213
BfuI GTATCC 1 cut(s) 719
BglI GCCNNNNNGGC 1 cut(s) 69
BglII AGATCT 1 cut(s) 347
BisI GCNGC 4 cut(s) 70, 201, 376, 884
BlsI GCNGC 4 cut(s) 71, 202, 377, 885
Bme1390I CCNGG 1 cut(s) 851
Bme18I GGWCC 1 cut(s) 733
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 733
BmrFI CCNGG 1 cut(s) 851
BmsI GCATC 3 cut(s) 322, 686, 691
BpmI CTGGAG 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 791
BpuMI CCSGG 1 cut(s) 851
BsaJI CCNNGG 4 cut(s) 64, 295, 383, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 742, 868
Bse1I ACTGG 1 cut(s) 446
BseDI CCNNGG 4 cut(s) 64, 295, 383, 519
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 742, 868
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 446
BseXI GCAGC 2 cut(s) 187, 895
BsgI GTGCAG 2 cut(s) 148, 902
Bsh1236I CGCG 1 cut(s) 318
BshFI GGCC 3 cut(s) 63, 162, 722
BsiSI CCGG 2 cut(s) 723, 851
BslI CCNNNNNNNGG 2 cut(s) 742, 868
BsmI GAATGC 1 cut(s) 896
BsnI GGCC 3 cut(s) 63, 162, 722
Bsp143I GATC 6 cut(s) 7, 46, 300, 347, 403, 471
Bsp19I CCATGG 3 cut(s) 64, 383, 519
BspACI CCGC 2 cut(s) 69, 376
BspANI GGCC 3 cut(s) 63, 162, 722
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 1 cut(s) 318
BspHI TCATGA 1 cut(s) 769
BspPI GGATC 3 cut(s) 2, 308, 466
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 4 cut(s) 64, 295, 383, 519
BssMI GATC 6 cut(s) 7, 46, 300, 347, 403, 471
BssT1I CCWWGG 4 cut(s) 64, 295, 383, 519
Bst4CI ACNGT 6 cut(s) 77, 332, 493, 545, 737, 915
Bst6I CTCTTC 1 cut(s) 777
BstAPI GCANNNNNTGC 1 cut(s) 819
BstDEI CTNAG 3 cut(s) 25, 243, 344
BstDSI CCRYGG 3 cut(s) 64, 383, 519
BstF5I GGATG 1 cut(s) 94
BstFNI CGCG 1 cut(s) 318
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 6 cut(s) 10, 49, 303, 350, 406, 474
BstMBI GATC 6 cut(s) 7, 46, 300, 347, 403, 471
BstMWI GCNNNNNNNGC 2 cut(s) 69, 819
BstSCI CCNGG 1 cut(s) 849
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 187, 895
BstX2I RGATCY 4 cut(s) 7, 300, 347, 471
BstYI RGATCY 4 cut(s) 7, 300, 347, 471
BsuI GTATCC 1 cut(s) 719
BsuRI GGCC 3 cut(s) 63, 162, 722
BtgI CCRYGG 3 cut(s) 64, 383, 519
BtgZI GCGATG 1 cut(s) 299
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 94
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 136
CciI TCATGA 1 cut(s) 769
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 733
Csp6I GTAC 4 cut(s) 29, 287, 439, 916
CviAII CATG 8 cut(s) 65, 85, 384, 502, 520, 718, 770, 826
CviQI GTAC 4 cut(s) 29, 287, 439, 916
DdeI CTNAG 3 cut(s) 25, 243, 344
DpnI GATC 6 cut(s) 9, 48, 302, 349, 405, 473
DpnII GATC 6 cut(s) 7, 46, 300, 347, 403, 471
DraI TTTAAA 1 cut(s) 657
EaeI YGGCCR 3 cut(s) 61, 160, 720
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
Eco130I CCWWGG 4 cut(s) 64, 295, 383, 519
Eco47I GGWCC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 480
EcoT14I CCWWGG 4 cut(s) 64, 295, 383, 519
ErhI CCWWGG 4 cut(s) 64, 295, 383, 519
FaeI CATG 8 cut(s) 68, 88, 387, 505, 523, 721, 773, 829
FatI CATG 8 cut(s) 64, 84, 383, 501, 519, 717, 769, 825
FauNDI CATATG 1 cut(s) 354
FbaI TGATCA 1 cut(s) 46
FblI GTMKAC 2 cut(s) 237, 327
Fnu4HI GCNGC 4 cut(s) 70, 201, 376, 884
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 70, 201, 376, 884
FspI TGCGCA 1 cut(s) 135
GlaI GCGC 1 cut(s) 135
GluI GCNGC 4 cut(s) 70, 201, 376, 884
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 3 cut(s) 63, 162, 722
HapII CCGG 2 cut(s) 723, 851
HhaI GCGC 1 cut(s) 136
Hin1II CATG 8 cut(s) 68, 88, 387, 505, 523, 721, 773, 829
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HincII GTYRAC 2 cut(s) 238, 328
HindII GTYRAC 2 cut(s) 238, 328
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 3 cut(s) 239, 260, 338
HpaII CCGG 2 cut(s) 723, 851
HphI GGTGA 2 cut(s) 347, 507
Hpy166II GTNNAC 5 cut(s) 238, 289, 328, 439, 489
Hpy188I TCNGA 2 cut(s) 244, 634
Hpy188III TCNNGA 4 cut(s) 50, 770, 779, 808
Hpy8I GTNNAC 5 cut(s) 238, 289, 328, 439, 489
HpyAV CCTTC 2 cut(s) 226, 547
HpyCH4III ACNGT 6 cut(s) 77, 332, 493, 545, 737, 915
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 7 cut(s) 129, 612, 647, 717, 789, 883, 907
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 819
HpyF3I CTNAG 3 cut(s) 25, 243, 344
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 8 cut(s) 68, 88, 387, 505, 523, 721, 773, 829
HspAI GCGC 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 6 cut(s) 7, 46, 300, 347, 403, 471
LpnPI CCDG 7 cut(s) 141, 213, 427, 609, 736, 864, 877
Lsp1109I GCAGC 2 cut(s) 187, 895
LweI GCATC 3 cut(s) 322, 686, 691
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 5 cut(s) 77, 495, 513, 536, 737
MalI GATC 6 cut(s) 9, 48, 302, 349, 405, 473
MboI GATC 6 cut(s) 7, 46, 300, 347, 403, 471
MboII GAAGA 3 cut(s) 255, 794, 866
MflI RGATCY 4 cut(s) 7, 300, 347, 471
MlsI TGGCCA 2 cut(s) 63, 162
MluCI AATT 8 cut(s) 118, 165, 177, 191, 223, 394, 399, 921
MluNI TGGCCA 2 cut(s) 63, 162
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 6 cut(s) 238, 438, 571, 592, 682, 827
Mox20I TGGCCA 2 cut(s) 63, 162
MscI TGGCCA 2 cut(s) 63, 162
MseI TTAA 4 cut(s) 168, 393, 398, 656
Msp20I TGGCCA 2 cut(s) 63, 162
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 2 cut(s) 723, 851
MspR9I CCNGG 1 cut(s) 851
Mva1269I GAATGC 1 cut(s) 896
MvnI CGCG 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 69, 819
NciI CCSGG 1 cut(s) 851
NcoI CCATGG 3 cut(s) 64, 383, 519
NdeI CATATG 1 cut(s) 354
NdeII GATC 6 cut(s) 7, 46, 300, 347, 403, 471
NlaIII CATG 8 cut(s) 68, 88, 387, 505, 523, 721, 773, 829
NmuCI GTSAC 3 cut(s) 495, 513, 737
NsbI TGCGCA 1 cut(s) 135
PagI TCATGA 1 cut(s) 769
PctI GAATGC 1 cut(s) 896
PfeI GAWTC 2 cut(s) 260, 338
PflFI GACNNNGTC 2 cut(s) 493, 511
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 4 cut(s) 71, 202, 377, 885
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PshBI ATTAAT 2 cut(s) 168, 393
PsiI TTATAA 1 cut(s) 426
PspPI GGNCC 1 cut(s) 733
PsuI RGATCY 4 cut(s) 7, 300, 347, 471
PsyI GACNNNGTC 2 cut(s) 493, 511
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 4 cut(s) 30, 288, 440, 917
RsaNI GTAC 4 cut(s) 29, 287, 439, 916
SalI GTCGAC 2 cut(s) 236, 326
SaqAI TTAA 4 cut(s) 168, 393, 398, 656
SatI GCNGC 4 cut(s) 70, 201, 376, 884
Sau3AI GATC 6 cut(s) 7, 46, 300, 347, 403, 471
Sau96I GGNCC 1 cut(s) 733
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 851
SfaNI GCATC 3 cut(s) 322, 686, 691
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
Sse9I AATT 8 cut(s) 118, 165, 177, 191, 223, 394, 399, 921
SsiI CCGC 2 cut(s) 69, 376
StyD4I CCNGG 1 cut(s) 849
StyI CCWWGG 4 cut(s) 64, 295, 383, 519
TaaI ACNGT 6 cut(s) 77, 332, 493, 545, 737, 915
TaiI ACGT 1 cut(s) 294
TaqI TCGA 4 cut(s) 196, 237, 327, 680
TasI AATT 8 cut(s) 118, 165, 177, 191, 223, 394, 399, 921
TatI WGTACW 2 cut(s) 286, 438
TauI GCSGC 2 cut(s) 72, 378
TfiI GAWTC 2 cut(s) 260, 338
Tru1I TTAA 4 cut(s) 168, 393, 398, 656
Tru9I TTAA 4 cut(s) 168, 393, 398, 656
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 3 cut(s) 495, 513, 737
TseI GCWGC 2 cut(s) 200, 883
Tsp45I GTSAC 3 cut(s) 495, 513, 737
TspDTI ATGAA 5 cut(s) 31, 69, 73, 105, 351
TspRI CASTG 1 cut(s) 136
Tth111I GACNNNGTC 2 cut(s) 493, 511
VpaK11BI GGWCC 1 cut(s) 733
VspI ATTAAT 2 cut(s) 168, 393
XapI RAATTY 2 cut(s) 191, 223
XmiI GTMKAC 2 cut(s) 237, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.