RchiOBHm_Chr1g0327571

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017746
Physical Location & Seq
Reverse (-)
16441121 .. 16442323
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55707

Sequence Viewer

Length: 444 bp
ATGTGGACGGTGTCACACATGATGACTTTGTCACCCATGGAGAAAGTTATTAGTTACTACCGTGCTTCGAAGGTGGCTGACAACTTGAATGATGAGGATTTGCGTTGTTTCTTGGAGGAACAGCTGTGCAAGGAGAAAGCAGGGCTGTCAGAGAAAACTATTGCAAGGCGTTTAAAAACAAAGGTTTATGTAGCATCGAAAGTGGAGGAAAACCCCGATGCTTGTAGTATTTGCATGGCCGGATACAAGGACCGTGACAAGGTTGCGAGTTTATACTATTGTTGTCATGAGTATCACGAAGAGTGCATAAAGGAGTGGCTTCTCAAGAGCAACCTCTGCCCCATGTGTAGAGCATTAGCTATAATCCCGGAAGATTACCCCTGTTGGGAAGCTGATGTGCAGCTACACCAAGCATTCTTTCTGCATTTACAGTACGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

147

Amino Acids

17.16

Weight (kDa)

5.73

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 74 - 117 4.7e-12 Ring finger domain
zf-rbx1 PF12678 74 - 117 2.3e-07 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 75 - 116 8.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 75 - 116 3.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 75 - 103 6.9e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 237
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 259, 385
AgsI TTSAA 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AluBI AGCT 4 cut(s) 124, 359, 392, 403
AluI AGCT 4 cut(s) 124, 359, 392, 403
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 1 cut(s) 400
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 24
AsuII TTCGAA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 412
BciVI GTATCC 1 cut(s) 236
BcnI CCSGG 1 cut(s) 368
BfuI GTATCC 1 cut(s) 236
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 368
BmsI GCATC 2 cut(s) 203, 208
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 385
BseDI CCNNGG 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 259, 385
BseXI GCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 419
BshFI GGCC 1 cut(s) 239
BsiSI CCGG 2 cut(s) 240, 368
BslI CCNNNNNNNGG 2 cut(s) 259, 385
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 1 cut(s) 239
Bsp119I TTCGAA 1 cut(s) 68
Bsp19I CCATGG 1 cut(s) 36
BspANI GGCC 1 cut(s) 239
BspHI TCATGA 1 cut(s) 286
BspT104I TTCGAA 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 36
Bst4CI ACNGT 4 cut(s) 10, 62, 254, 432
Bst6I CTCTTC 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 68
BstDSI CCRYGG 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 412
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 36
CciI TCATGA 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 433
CviAII CATG 5 cut(s) 19, 37, 235, 287, 343
CviJI RGCY 8 cut(s) 77, 124, 145, 239, 319, 359, 392, 403
CviKI_1 RGCY 8 cut(s) 77, 124, 145, 239, 319, 359, 392, 403
CviQI GTAC 1 cut(s) 433
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 5 cut(s) 22, 40, 238, 290, 346
FaiI YATR 9 cut(s) 20, 38, 189, 236, 274, 288, 308, 344, 362
FatI CATG 5 cut(s) 18, 36, 234, 286, 342
Fnu4HI GCNGC 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 401
GluI GCNGC 1 cut(s) 401
HaeIII GGCC 1 cut(s) 239
HapII CCGG 2 cut(s) 240, 368
Hin1II CATG 5 cut(s) 22, 40, 238, 290, 346
HpaII CCGG 2 cut(s) 240, 368
HphI GGTGA 1 cut(s) 24
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 3 cut(s) 287, 296, 325
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 4 cut(s) 10, 62, 254, 432
HpyCH4V TGCA 6 cut(s) 129, 164, 234, 306, 400, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
Hsp92II CATG 5 cut(s) 22, 40, 238, 290, 346
LpnPI CCDG 4 cut(s) 126, 253, 381, 394
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 203, 208
MaeIII GTNAC 4 cut(s) 12, 30, 53, 254
MboII GAAGA 2 cut(s) 311, 383
MluCI AATT 1 cut(s) 438
MnlI CCTC 4 cut(s) 88, 109, 199, 344
MseI TTAA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 124
MspI CCGG 2 cut(s) 240, 368
MspR9I CCNGG 1 cut(s) 368
Mva1269I GAATGC 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 368
NcoI CCATGG 1 cut(s) 36
NlaIII CATG 5 cut(s) 22, 40, 238, 290, 346
NmuCI GTSAC 3 cut(s) 12, 30, 254
NspV TTCGAA 1 cut(s) 68
PagI TCATGA 1 cut(s) 286
PctI GAATGC 1 cut(s) 413
PflFI GACNNNGTC 2 cut(s) 10, 28
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 1 cut(s) 402
PspPI GGNCC 1 cut(s) 250
PsyI GACNNNGTC 2 cut(s) 10, 28
PvuII CAGCTG 1 cut(s) 124
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 1 cut(s) 401
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 368
SetI ASST 8 cut(s) 75, 126, 186, 264, 336, 361, 394, 405
SfaNI GCATC 2 cut(s) 203, 208
SfuI TTCGAA 1 cut(s) 68
SinI GGWCC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 4 cut(s) 10, 62, 254, 432
TaqI TCGA 2 cut(s) 68, 197
TasI AATT 1 cut(s) 438
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TseFI GTSAC 3 cut(s) 12, 30, 254
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 3 cut(s) 12, 30, 254
Tth111I GACNNNGTC 2 cut(s) 10, 28
VpaK11BI GGWCC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.