Prupe.2G196000_v2.0.a1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0015371
Physical Location & Seq
Forward (+)
23347783 .. 23350217
2435 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G196000.1

Sequence Viewer

Length: 1614 bp
ATGGATGTTGCTCATGCTAGTAGTAATAGTAGTATAGACATGGACAATGAGAGATTGACGGCGGAGATGGCTTTCAAGGACTCGTCCTCCGCCGTCATCAAAATCCGCCAACGCTTGCCGGACTTCCTGCAGTCCGTCAAGCTCAAGTACGTCAAGCTCGGCTACGGTTACTCTTGTAACGCAGCCACCATTCTCTTGTTCCTCTTCATCCTACCTCTCTTCGTGTCCGTAGTGATACAGCTCACCGGTCTGGAGTTGAACCGTATCACCGAGCTCTGGACCAACCAGGCCGTCCGCCTCGAGAGCATCGACGCCGCCACCCGCCTCGCCGGTTCCGGCGTCCTCCTCTTCTTCCTCGGCCTCTACTGGGCCAAGCGGTCCAGACCGGTCTACCTCGTCGACTTCTCGTGCTACAAACCGGACGACCAACACAAGCTGTCGGTCGACTCGTTTCTAAAAATGACCGAAGAAAGCGGCGCATTTGCTCAGGACACTTTTCAATTCCAGACCCGGATTGCGAACCGGTCCGGTCTAGGCGACGAGACGTACTTGCCGCCCGGAATTACATCCAAACCGCCTCAGCTGTCCATGGAGCAAGCCCGGTCCGAGGCCGAGACGGTTATGTTCGGCGCGCTCAACTCGCTCTTCGAAAAAACCGGAGTGAAACCGAGCGAAATCGACATCCTCATCGTAAATTGCAGCCTGTTCAACCCGACGCCGTCGCTCTCCGCCATGATCGTCAACCACTACAAGCTCAAAACCGACATTAAAAGCTACAACCTCGGTGGTATGGGCTGCAGCGCCGGTCTGATCTCCATCGACCTCGCCAAGGACCTCCTCAAAGCAAACCCCAACTCCTACGCCGTCGTAGTGAGCACCGAGAACATCACCTTGAACTGGTACTTCGGAAACGACAAGTCGATGCTGCTCTGCAACTGTATTTTCCGAATGGGCGGCGCCGCGGTTCTACTGTCGAACAAAAGCCGGGACCGAACCCGGTCCAAGTACGAACTCGTCCACACCGTCCGGACCCACAAGGGCGCGGACGACAGGAGTTACAATTGCGTTTACCAGCGGGAGGACGACAAGGGAACAATCGGCGTGTCTCTCGCGCGCGAGCTGATGGCGGTTGCCGGGGATTCGTTGAAAACGAACATCACGACGCTGGGCCCACTGGTGCTGCCGTTTTCCGAGCAGGTGCTCTTCTTCATCACGCTGATCCGGAAGAAGGTGTTCAAGGCAAAGGTGAAGCCGTACATACCGGATTTCAAGCTCGCGTTCGAGCATTTCTGCATTCACGCCGGAGGGCGGGCCGTGCTGGACGAGTTGGAGAAGAACCTGCAACTCACTGAGTGGCACATGGAGCCTTCCAGAATGACACTGCACCGCTTCGGCAACACGTCGAGTAGCTCGTTGTGGTACGAGCTGGCGTACGCGGAGGCCAAGGGCCGGGTCGGGCGGGGTGACCGAGTCTGGCAGATCGCATTCGGGTCGGGTTTCAAGTGTAACAGCGCGGTTTGGCGGGCGCTCCGGCCTATTTCGGTCGGGGATGGGTTGGGTAACCCGTGGATGGACTCGATTGATAGGTACCCGGTTAAAGTTTCAATCCGTTAG

Protein Analysis

538

Amino Acids

59.99

Weight (kDa)

8.92

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01120
fragaria_vesca FvH4_7g17440
malus_domestica MD01G1087400.v1.1 MD07G1157600.v1.1
prunus_persica Prupe.2G196000_v2.0.a1
pyrus_communis pycom01g11140 pycom07g15410
rosa_chinensis RchiOBHm_Chr1g0360891
rosa_laevigata RLG00000027773 RLG00000027775
rosa_multiflora Rmu_sc0000677.1_g000044
rosa_rugosa Rorug01G0282900
rosa_samantha Rh1AG294400 Rh1BG259400 Rh1DG289400
rosa_wichuraiana Rw1G026040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1186
Acc36I ACCTGC 2 cut(s) 1186, 1347
Acc65I GGTACC 1 cut(s) 1587
AccB1I GGYRCC 2 cut(s) 956, 1587
AccI GTMKAC 3 cut(s) 390, 399, 444
AccII CGCG 9 cut(s) 632, 962, 1043, 1112, 1114, 1116, 1277, 1436, 1514
AccIII TCCGGA 2 cut(s) 1026, 1221
AclWI GGATC 1 cut(s) 1213
AcyI GRCGYC 4 cut(s) 312, 339, 716, 957
AdeI CACNNNGTG 1 cut(s) 1353
AfaI GTAC 8 cut(s) 149, 548, 902, 1007, 1256, 1421, 1433, 1589
AflIII ACRYGT 1 cut(s) 1398
AgeI ACCGGT 3 cut(s) 245, 385, 522
AjiI CACGTC 1 cut(s) 1401
AjnI CCWGG 1 cut(s) 285
Alw21I GWGCWC 3 cut(s) 276, 878, 1203
Alw26I GTCTC 3 cut(s) 536, 608, 1110
AlwI GGATC 1 cut(s) 1213
Ama87I CYCGRG 1 cut(s) 299
Aor13HI TCCGGA 2 cut(s) 1026, 1221
AoxI GGCC 9 cut(s) 288, 358, 369, 609, 1168, 1311, 1440, 1447, 1532
ApaI GGGCCC 1 cut(s) 1172
ApeKI GCWGC 6 cut(s) 182, 699, 795, 798, 925, 1180
AsiGI ACCGGT 3 cut(s) 245, 385, 522
Asp700I GAANNNNTTC 1 cut(s) 1232
Asp718I GGTACC 1 cut(s) 1587
AsuC2I CCSGG 8 cut(s) 511, 558, 601, 986, 997, 1135, 1451, 1592
AsuHPI GGTGA 5 cut(s) 235, 259, 880, 1258, 1475
AsuII TTCGAA 1 cut(s) 648
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 8 cut(s) 279, 378, 525, 603, 832, 988, 999, 1029
BaeGI GKGCMC 1 cut(s) 1172
BanI GGYRCC 2 cut(s) 956, 1587
BanII GRGCYC 2 cut(s) 276, 1172
BauI CACGAG 1 cut(s) 406
Bbv12I GWGCWC 3 cut(s) 276, 878, 1203
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 6 cut(s) 194, 711, 782, 810, 912, 1167
BccI CCATC 5 cut(s) 61, 824, 1117, 1544, 1564
BceAI ACGGC 8 cut(s) 75, 77, 275, 703, 848, 1168, 1237, 1298
BcgI CGANNNNNNTGC 2 cut(s) 1473, 1507
BciT130I CCWGG 1 cut(s) 287
BcnI CCSGG 8 cut(s) 511, 558, 601, 986, 997, 1135, 1451, 1592
BcoDI GTCTC 3 cut(s) 536, 608, 1110
BfaI CTAG 2 cut(s) 18, 533
BfmI CTRYAG 2 cut(s) 128, 796
BfoI RGCGCY 3 cut(s) 804, 960, 1529
BfuAI ACCTGC 2 cut(s) 1186, 1347
Bme1390I CCNGG 9 cut(s) 287, 511, 558, 601, 986, 997, 1135, 1451, 1592
Bme18I GGWCC 8 cut(s) 279, 378, 525, 603, 832, 988, 999, 1029
BmeT110I CYCGRG 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 1401
BmiI GGNNCC 7 cut(s) 334, 958, 989, 1031, 1170, 1365, 1589
BmrFI CCNGG 9 cut(s) 287, 511, 558, 601, 986, 997, 1135, 1451, 1592
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 2 cut(s) 315, 912
BmuI ACTGGG 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 2 cut(s) 486, 579
Bpu14I TTCGAA 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 8 cut(s) 511, 558, 601, 986, 997, 1135, 1451, 1592
BsaBI GATNNNNATC 1 cut(s) 815
BsaHI GRCGYC 4 cut(s) 312, 339, 716, 957
BsaJI CCNNGG 9 cut(s) 355, 588, 606, 781, 828, 960, 1134, 1443, 1565
BsaWI WCCGGW 9 cut(s) 245, 385, 418, 522, 527, 656, 1026, 1221, 1261
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 839, 869
Bse118I RCCGGY 5 cut(s) 245, 329, 385, 522, 803
Bse1I ACTGG 3 cut(s) 371, 902, 1179
Bse8I GATNNNNATC 1 cut(s) 815
BseAI TCCGGA 2 cut(s) 1026, 1221
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 9 cut(s) 355, 588, 606, 781, 828, 960, 1134, 1443, 1565
BseGI GGATG 6 cut(s) 10, 207, 566, 681, 1555, 1575
BseJI GATNNNNATC 1 cut(s) 815
BseMII CTCAG 3 cut(s) 500, 593, 1341
BseNI ACTGG 3 cut(s) 371, 902, 1179
BsePI GCGCGC 2 cut(s) 630, 1112
BseRI GAGGAG 2 cut(s) 335, 827
BseSI GKGCMC 1 cut(s) 1172
BseXI GCAGC 6 cut(s) 194, 711, 782, 810, 912, 1167
BseYI CCCAGC 1 cut(s) 1165
BsgI GTGCAG 1 cut(s) 1367
Bsh1236I CGCG 9 cut(s) 632, 962, 1043, 1112, 1114, 1116, 1277, 1436, 1514
Bsh1285I CGRYCG 2 cut(s) 444, 1545
BshFI GGCC 9 cut(s) 290, 360, 371, 611, 1170, 1313, 1442, 1449, 1534
BshNI GGYRCC 2 cut(s) 956, 1587
BshTI ACCGGT 3 cut(s) 245, 385, 522
BsiEI CGRYCG 2 cut(s) 444, 1545
BsiHKAI GWGCWC 3 cut(s) 276, 878, 1203
BsiHKCI CYCGRG 1 cut(s) 299
BsiWI CGTACG 1 cut(s) 1431
BslFI GGGAC 1 cut(s) 1001
BsmAI GTCTC 3 cut(s) 536, 608, 1110
BsmBI CGTCTC 2 cut(s) 536, 608
BsmFI GGGAC 1 cut(s) 1001
BsmI GAATGC 2 cut(s) 1293, 1484
BsnI GGCC 9 cut(s) 290, 360, 371, 611, 1170, 1313, 1442, 1449, 1534
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 648
Bsp120I GGGCCC 1 cut(s) 1168
Bsp1286I GDGCHC 4 cut(s) 276, 878, 1172, 1203
Bsp13I TCCGGA 2 cut(s) 1026, 1221
Bsp143I GATC 4 cut(s) 735, 810, 1218, 1479
Bsp19I CCATGG 1 cut(s) 588
BspANI GGCC 9 cut(s) 290, 360, 371, 611, 1170, 1313, 1442, 1449, 1534
BspCNI CTCAG 3 cut(s) 499, 592, 1342
BspEI TCCGGA 2 cut(s) 1026, 1221
BspFNI CGCG 9 cut(s) 632, 962, 1043, 1112, 1114, 1116, 1277, 1436, 1514
BspLI GGNNCC 7 cut(s) 334, 958, 989, 1031, 1170, 1365, 1589
BspMAI CTGCAG 2 cut(s) 132, 800
BspMI ACCTGC 2 cut(s) 1186, 1347
BspPI GGATC 1 cut(s) 1213
BspQI GCTCTTC 2 cut(s) 650, 1208
BspT104I TTCGAA 1 cut(s) 648
BspT107I GGYRCC 2 cut(s) 956, 1587
BsrFI RCCGGY 5 cut(s) 245, 329, 385, 522, 803
BsrI ACTGG 3 cut(s) 371, 902, 1179
BssAI RCCGGY 5 cut(s) 245, 329, 385, 522, 803
BssECI CCNNGG 9 cut(s) 355, 588, 606, 781, 828, 960, 1134, 1443, 1565
BssHII GCGCGC 2 cut(s) 630, 1112
BssMI GATC 4 cut(s) 735, 810, 1218, 1479
BssNI GRCGYC 4 cut(s) 312, 339, 716, 957
BssSI CACGAG 1 cut(s) 406
BssT1I CCWWGG 3 cut(s) 588, 828, 1443
Bst2BI CACGAG 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 6 cut(s) 167, 263, 619, 938, 972, 1024
Bst6I CTCTTC 5 cut(s) 209, 224, 353, 650, 1208
BstACI GRCGYC 4 cut(s) 312, 339, 716, 957
BstBI TTCGAA 1 cut(s) 648
BstC8I GCNNGC 9 cut(s) 116, 597, 632, 1114, 1118, 1275, 1311, 1428, 1524
BstDEI CTNAG 3 cut(s) 486, 579, 1350
BstDSI CCRYGG 3 cut(s) 588, 960, 1565
BstEII GGTNACC 2 cut(s) 1463, 1559
BstENI CCTNNNNNAGG 1 cut(s) 827
BstF5I GGATG 6 cut(s) 10, 207, 566, 681, 1555, 1575
BstFNI CGCG 9 cut(s) 632, 962, 1043, 1112, 1114, 1116, 1277, 1436, 1514
BstH2I RGCGCY 3 cut(s) 804, 960, 1529
BstKTI GATC 4 cut(s) 738, 813, 1221, 1482
BstMAI GTCTC 3 cut(s) 536, 608, 1110
BstMBI GATC 4 cut(s) 735, 810, 1218, 1479
BstMCI CGRYCG 2 cut(s) 444, 1545
BstMWI GCNNNNNNNGC 5 cut(s) 68, 303, 640, 1315, 1363
BstNI CCWGG 1 cut(s) 287
BstPI GGTNACC 2 cut(s) 1463, 1559
BstSCI CCNGG 9 cut(s) 285, 509, 556, 599, 984, 995, 1133, 1449, 1590
BstSFI CTRYAG 2 cut(s) 128, 796
BstSLI GKGCMC 1 cut(s) 1172
BstUI CGCG 9 cut(s) 632, 962, 1043, 1112, 1114, 1116, 1277, 1436, 1514
BstV1I GCAGC 6 cut(s) 194, 711, 782, 810, 912, 1167
BsuRI GGCC 9 cut(s) 290, 360, 371, 611, 1170, 1313, 1442, 1449, 1534
BtgI CCRYGG 3 cut(s) 588, 960, 1565
BtrI CACGTC 1 cut(s) 1401
BtsCI GGATG 6 cut(s) 10, 207, 566, 681, 1555, 1575
BtsI GCAGTG 1 cut(s) 1379
BtsIMutI CAGTG 3 cut(s) 1172, 1347, 1379
BveI ACCTGC 2 cut(s) 1186, 1347
Cac8I GCNNGC 9 cut(s) 116, 597, 632, 1114, 1118, 1275, 1311, 1428, 1524
Cfr10I RCCGGY 5 cut(s) 245, 329, 385, 522, 803
Cfr42I CCGCGG 1 cut(s) 963
CpoI CGGWCCG 2 cut(s) 525, 603
CseI GACGC 4 cut(s) 320, 328, 724, 1171
Csp6I GTAC 8 cut(s) 148, 547, 901, 1006, 1255, 1420, 1432, 1588
CspAI ACCGGT 3 cut(s) 245, 385, 522
CspCI CAANNNNNGTGG 2 cut(s) 766, 801
CspI CGGWCCG 2 cut(s) 525, 603
CviAII CATG 5 cut(s) 14, 40, 589, 733, 1360
CviQI GTAC 8 cut(s) 148, 547, 901, 1006, 1255, 1420, 1432, 1588
DdeI CTNAG 3 cut(s) 486, 579, 1350
DinI GGCGCC 1 cut(s) 958
DpnI GATC 4 cut(s) 737, 812, 1220, 1481
DpnII GATC 4 cut(s) 735, 810, 1218, 1479
DraIII CACNNNGTG 1 cut(s) 1353
Eam1104I CTCTTC 5 cut(s) 209, 224, 353, 650, 1208
EarI CTCTTC 5 cut(s) 209, 224, 353, 650, 1208
EciI GGCGGA 5 cut(s) 77, 79, 95, 284, 718
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 3 cut(s) 588, 828, 1443
Eco24I GRGCYC 2 cut(s) 276, 1172
Eco47I GGWCC 8 cut(s) 279, 378, 525, 603, 832, 988, 999, 1029
Eco53kI GAGCTC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 299
Eco91I GGTNACC 2 cut(s) 1463, 1559
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 827
EcoO109I RGGNCCY 1 cut(s) 832
EcoO65I GGTNACC 2 cut(s) 1463, 1559
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 3 cut(s) 588, 828, 1443
EcoT38I GRGCYC 2 cut(s) 276, 1172
EgeI GGCGCC 1 cut(s) 958
EheI GGCGCC 1 cut(s) 958
ErhI CCWWGG 3 cut(s) 588, 828, 1443
Esp3I CGTCTC 2 cut(s) 536, 608
FaeI CATG 5 cut(s) 17, 43, 592, 736, 1363
FaiI YATR 9 cut(s) 15, 35, 41, 590, 623, 734, 791, 1259, 1361
FaqI GGGAC 1 cut(s) 1001
FatI CATG 5 cut(s) 13, 39, 588, 732, 1359
FauI CCCGC 5 cut(s) 329, 1068, 1302, 1452, 1515
FblI GTMKAC 3 cut(s) 390, 399, 444
FokI GGATG 6 cut(s) 17, 194, 553, 668, 1562, 1582
FriOI GRGCYC 2 cut(s) 276, 1172
FspBI CTAG 2 cut(s) 18, 533
GsaI CCCAGC 1 cut(s) 1169
GsuI CTGGAG 1 cut(s) 272
HaeII RGCGCY 3 cut(s) 804, 960, 1529
HaeIII GGCC 9 cut(s) 290, 360, 371, 611, 1170, 1313, 1442, 1449, 1534
HgaI GACGC 4 cut(s) 320, 328, 724, 1171
Hin1I GRCGYC 4 cut(s) 312, 339, 716, 957
Hin1II CATG 5 cut(s) 17, 43, 592, 736, 1363
HincII GTYRAC 3 cut(s) 400, 445, 742
HindII GTYRAC 3 cut(s) 400, 445, 742
HinfI GANTC 5 cut(s) 80, 446, 1139, 1470, 1574
HphI GGTGA 5 cut(s) 235, 259, 880, 1258, 1475
Hpy166II GTNNAC 6 cut(s) 391, 400, 445, 742, 1018, 1069
Hpy188I TCNGA 5 cut(s) 607, 810, 908, 947, 1192
Hpy8I GTNNAC 6 cut(s) 391, 400, 445, 742, 1018, 1069
Hpy99I CGWCG 8 cut(s) 314, 401, 542, 718, 724, 869, 1165, 1405
HpyAV CCTTC 2 cut(s) 1222, 1377
HpyCH4III ACNGT 6 cut(s) 167, 263, 619, 938, 972, 1024
HpyCH4IV ACGT 3 cut(s) 150, 545, 1400
HpyCH4V TGCA 7 cut(s) 130, 699, 798, 933, 1293, 1342, 1384
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 303, 640, 1315, 1363
HpyF3I CTNAG 3 cut(s) 486, 579, 1350
HpySE526I ACGT 3 cut(s) 150, 545, 1400
Hsp92I GRCGYC 4 cut(s) 312, 339, 716, 957
Hsp92II CATG 5 cut(s) 17, 43, 592, 736, 1363
KasI GGCGCC 1 cut(s) 956
Kpn2I TCCGGA 2 cut(s) 1026, 1221
KpnI GGTACC 1 cut(s) 1591
KspI CCGCGG 1 cut(s) 963
Kzo9I GATC 4 cut(s) 735, 810, 1218, 1479
LguI GCTCTTC 2 cut(s) 650, 1208
LmnI GCTCC 3 cut(s) 592, 1363, 1533
Lsp1109I GCAGC 6 cut(s) 194, 711, 782, 810, 912, 1167
LweI GCATC 2 cut(s) 315, 912
MaeI CTAG 2 cut(s) 18, 533
MaeII ACGT 3 cut(s) 150, 545, 1400
MaeIII GTNAC 6 cut(s) 167, 176, 1055, 1463, 1505, 1559
MalI GATC 4 cut(s) 737, 812, 1220, 1481
MauBI CGCGCGCG 1 cut(s) 1112
MboI GATC 4 cut(s) 735, 810, 1218, 1479
MfeI CAATTG 1 cut(s) 1060
MhlI GDGCHC 4 cut(s) 276, 878, 1172, 1203
MluCI AATT 4 cut(s) 500, 561, 694, 1060
Mly113I GGCGCC 1 cut(s) 957
MlyI GAGTC 4 cut(s) 74, 440, 1479, 1568
MmeI TCCRAC 1 cut(s) 1308
MroI TCCGGA 2 cut(s) 1026, 1221
MroXI GAANNNNTTC 1 cut(s) 1232
MseI TTAA 2 cut(s) 768, 1596
MspA1I CMGCKG 3 cut(s) 583, 962, 1075
MspR9I CCNGG 9 cut(s) 287, 511, 558, 601, 986, 997, 1135, 1451, 1592
MunI CAATTG 1 cut(s) 1060
Mva1269I GAATGC 2 cut(s) 1293, 1484
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 9 cut(s) 632, 962, 1043, 1112, 1114, 1116, 1277, 1436, 1514
MwoI GCNNNNNNNGC 5 cut(s) 68, 303, 640, 1315, 1363
NarI GGCGCC 1 cut(s) 957
NciI CCSGG 8 cut(s) 511, 558, 601, 986, 997, 1135, 1451, 1592
NcoI CCATGG 1 cut(s) 588
NdeII GATC 4 cut(s) 735, 810, 1218, 1479
NlaIII CATG 5 cut(s) 17, 43, 592, 736, 1363
NlaIV GGNNCC 7 cut(s) 334, 958, 989, 1031, 1170, 1365, 1589
NmeAIII GCCGAG 3 cut(s) 138, 336, 637
NmuCI GTSAC 1 cut(s) 1463
NspV TTCGAA 1 cut(s) 648
PaeR7I CTCGAG 1 cut(s) 299
PaqCI CACCTGC 1 cut(s) 1186
PauI GCGCGC 2 cut(s) 630, 1112
PciSI GCTCTTC 2 cut(s) 650, 1208
PcsI WCGNNNNNNNCGW 6 cut(s) 306, 446, 1020, 1148, 1157, 1409
PctI GAATGC 2 cut(s) 1293, 1484
PdmI GAANNNNTTC 1 cut(s) 1232
PfeI GAWTC 1 cut(s) 1139
Pfl23II CGTACG 1 cut(s) 1431
PflFI GACNNNGTC 2 cut(s) 718, 1469
PinAI ACCGGT 3 cut(s) 245, 385, 522
PleI GAGTC 4 cut(s) 74, 440, 1478, 1568
PluTI GGCGCC 1 cut(s) 960
PpsI GAGTC 4 cut(s) 74, 440, 1478, 1568
PpuMI RGGWCCY 1 cut(s) 832
Psp124BI GAGCTC 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 1 cut(s) 285
PspEI GGTNACC 2 cut(s) 1463, 1559
PspFI CCCAGC 1 cut(s) 1165
PspGI CCWGG 1 cut(s) 285
PspLI CGTACG 1 cut(s) 1431
PspN4I GGNNCC 7 cut(s) 334, 958, 989, 1031, 1170, 1365, 1589
PspOMI GGGCCC 1 cut(s) 1168
PspPPI RGGWCCY 1 cut(s) 832
PstI CTGCAG 2 cut(s) 132, 800
PsyI GACNNNGTC 2 cut(s) 718, 1469
PteI GCGCGC 2 cut(s) 630, 1112
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 8 cut(s) 149, 548, 902, 1007, 1256, 1421, 1433, 1589
RsaNI GTAC 8 cut(s) 148, 547, 901, 1006, 1255, 1420, 1432, 1588
Rsr2I CGGWCCG 2 cut(s) 525, 603
RsrII CGGWCCG 2 cut(s) 525, 603
SacI GAGCTC 1 cut(s) 276
SacII CCGCGG 1 cut(s) 963
SalI GTCGAC 2 cut(s) 398, 443
SapI GCTCTTC 2 cut(s) 650, 1208
SaqAI TTAA 2 cut(s) 768, 1596
Sau3AI GATC 4 cut(s) 735, 810, 1218, 1479
SchI GAGTC 4 cut(s) 74, 440, 1479, 1568
ScrFI CCNGG 9 cut(s) 287, 511, 558, 601, 986, 997, 1135, 1451, 1592
SduI GDGCHC 4 cut(s) 276, 878, 1172, 1203
SfaNI GCATC 2 cut(s) 315, 912
SfcI CTRYAG 2 cut(s) 128, 796
SfoI GGCGCC 1 cut(s) 958
Sfr274I CTCGAG 1 cut(s) 299
Sfr303I CCGCGG 1 cut(s) 963
SfuI TTCGAA 1 cut(s) 648
SgrBI CCGCGG 1 cut(s) 963
SinI GGWCC 8 cut(s) 279, 378, 525, 603, 832, 988, 999, 1029
SlaI CTCGAG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 143, 299
SmoI CTYRAG 2 cut(s) 143, 299
Sse9I AATT 4 cut(s) 500, 561, 694, 1060
SspDI GGCGCC 1 cut(s) 956
SspMI CTAG 2 cut(s) 18, 533
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 9 cut(s) 285, 509, 556, 599, 984, 995, 1133, 1449, 1590
StyI CCWWGG 3 cut(s) 588, 828, 1443
TaaI ACNGT 6 cut(s) 167, 263, 619, 938, 972, 1024
TaiI ACGT 3 cut(s) 153, 548, 1403
TaqII GACCGA 5 cut(s) 430, 479, 1005, 1482, 1531
TasI AATT 4 cut(s) 500, 561, 694, 1060
TauI GCSGC 5 cut(s) 317, 477, 556, 957, 962
TfiI GAWTC 1 cut(s) 1139
Tru1I TTAA 2 cut(s) 768, 1596
Tru9I TTAA 2 cut(s) 768, 1596
TscAI CASTG 3 cut(s) 1179, 1354, 1386
TseFI GTSAC 1 cut(s) 1463
TseI GCWGC 6 cut(s) 182, 699, 795, 798, 925, 1180
Tsp45I GTSAC 1 cut(s) 1463
TspDTI ATGAA 2 cut(s) 196, 1198
TspGWI ACGGA 3 cut(s) 124, 217, 1598
TspRI CASTG 3 cut(s) 1179, 1354, 1386
Tth111I GACNNNGTC 2 cut(s) 718, 1469
VpaK11BI GGWCC 8 cut(s) 279, 378, 525, 603, 832, 988, 999, 1029
XagI CCTNNNNNAGG 1 cut(s) 827
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 3 cut(s) 390, 399, 444
XmnI GAANNNNTTC 1 cut(s) 1232
XspI CTAG 2 cut(s) 18, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.