pycom07g15410

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015371
Physical Location & Seq
Forward (+)
17728737 .. 17729528
792 bp
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UTR
Exon/CDS
Intron
pycom07g15410.1

Sequence Viewer

Length: 792 bp
ATGGATGCTGTTTCTGTTGATACTAGCAGCAGTAATAGTAATAACAGTAGTAGCATAAACATGGACAAGGAGAGATTGACGGCGGAGATGGCGTTCAAGGACTCGTCCTCCGCCGTCATCAAAATCCGGCAGCGCCTGCCGGACTTCTTACAGTCTGTCAAGCTCAAGTACGTCAAGCTCGGCTATGGCTACACCGTCAACGCAGCCACCATTATTTTGTTCATACTCATTTTACCGCTCTTCATCTCGGTATTAATCCAGCTCACCGGTCTGGAGCTGGAACGGATCACAGAGCTCTGGACCAACCAGGCTGTCCGGATCGAACGCATCGACGCCGCCACCAGGCTCGCCGGTTCAGGGGTCCTCCTCTTCTTCTTCGGTCTCTACTGGGCCAAGCGGTCTAGACCAGTTTACCTGGTCGACTTCTCATGCTACAAACCGGAAGACGAACGCAAAATGTCCGTTGAGTCGTTTCTAAAAATGACTGAAGACAGCGGCGCATTTGCTCCGGACACGGTTAACTTCCAGACCCGCATTTCGAACCGGTCCGGTCTAGGCGACGAGACGTACTTGCCGCGTGGAATCACCTCCAACCCGCCACAGCTGTCCATGGAGCAAGCCCGGTCAGAGGCCGAGGCGGTCATGTTCGGCGCGCTCGACTCACTCTTCAAAAAAACTGGAATCAAACCGAACCAAATCGACATCCTCATCGTGAACTGCAGCCTGTTCAACCCGACGCCGTCGCTGTCGTCCATGATCGATCAACCACTACAAGCTAAAAACCGACATTAA

Protein Analysis

264

Amino Acids

29.4

Weight (kDa)

7.69

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 130 - 253 5.2e-49 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015371)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01120
fragaria_vesca FvH4_7g17440
malus_domestica MD01G1087400.v1.1 MD07G1157600.v1.1
prunus_persica Prupe.2G196000_v2.0.a1
pyrus_communis pycom01g11140 pycom07g15410
rosa_chinensis RchiOBHm_Chr1g0360891
rosa_laevigata RLG00000027773 RLG00000027775
rosa_multiflora Rmu_sc0000677.1_g000044
rosa_rugosa Rorug01G0282900
rosa_samantha Rh1AG294400 Rh1BG259400 Rh1DG289400
rosa_wichuraiana Rw1G026040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 420
AccII CGCG 2 cut(s) 577, 653
AccIII TCCGGA 2 cut(s) 315, 508
AclWI GGATC 2 cut(s) 293, 326
AcuI CTGAAG 1 cut(s) 507
AcyI GRCGYC 2 cut(s) 333, 737
AfaI GTAC 2 cut(s) 170, 569
AfiI CCNNNNNNNGG 3 cut(s) 342, 357, 628
AgeI ACCGGT 2 cut(s) 266, 543
AgsI TTSAA 3 cut(s) 97, 670, 730
AjnI CCWGG 3 cut(s) 306, 341, 414
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
AluBI AGCT 7 cut(s) 163, 178, 262, 277, 295, 604, 776
AluI AGCT 7 cut(s) 163, 178, 262, 277, 295, 604, 776
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 386, 557
AlwI GGATC 2 cut(s) 293, 326
AlwNI CAGNNNCTG 1 cut(s) 136
Aor13HI TCCGGA 2 cut(s) 315, 508
AoxI GGCC 2 cut(s) 390, 630
ApeKI GCWGC 4 cut(s) 27, 130, 203, 720
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
AseI ATTAAT 1 cut(s) 254
AsiGI ACCGGT 2 cut(s) 266, 543
AspLEI GCGC 4 cut(s) 135, 500, 653, 655
AspS9I GGNCC 4 cut(s) 300, 361, 390, 546
AsuC2I CCSGG 1 cut(s) 622
AsuHPI GGTGA 2 cut(s) 256, 577
AsuII TTCGAA 1 cut(s) 539
AvaII GGWCC 3 cut(s) 300, 361, 546
BanII GRGCYC 1 cut(s) 297
BbsI GAAGAC 2 cut(s) 450, 495
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 4 cut(s) 39, 142, 215, 732
BccI CCATC 1 cut(s) 82
BceAI ACGGC 3 cut(s) 96, 98, 724
BciT130I CCWGG 3 cut(s) 308, 343, 416
BcnI CCSGG 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 386, 557
BfaI CTAG 3 cut(s) 24, 402, 554
BfmI CTRYAG 1 cut(s) 718
BfoI RGCGCY 1 cut(s) 136
BisI GCNGC 7 cut(s) 28, 131, 204, 336, 496, 575, 721
BlsI GCNGC 7 cut(s) 29, 132, 205, 337, 497, 576, 722
Bme1390I CCNGG 4 cut(s) 308, 343, 416, 622
Bme18I GGWCC 3 cut(s) 300, 361, 546
BmgT120I GGNCC 4 cut(s) 300, 361, 390, 546
BmiI GGNNCC 1 cut(s) 362
BmrFI CCNGG 4 cut(s) 308, 343, 416, 622
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 1 cut(s) 336
BmuI ACTGGG 1 cut(s) 397
BpiI GAAGAC 2 cut(s) 450, 495
BpmI CTGGAG 1 cut(s) 293
Bpu14I TTCGAA 1 cut(s) 539
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 622
Bsa29I ATCGAT 1 cut(s) 759
BsaHI GRCGYC 2 cut(s) 333, 737
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 609, 633
BsaWI WCCGGW 6 cut(s) 266, 315, 439, 508, 543, 548
BsaXI ACNNNNNCTCC 4 cut(s) 77, 92, 107, 122
Bsc4I CCNNNNNNNGG 3 cut(s) 342, 357, 628
Bse118I RCCGGY 3 cut(s) 266, 350, 543
Bse1I ACTGG 3 cut(s) 392, 407, 682
BseAI TCCGGA 2 cut(s) 315, 508
BseBI CCWGG 3 cut(s) 308, 343, 416
BseCI ATCGAT 1 cut(s) 759
BseDI CCNNGG 2 cut(s) 609, 633
BseGI GGATG 2 cut(s) 10, 702
BseLI CCNNNNNNNGG 3 cut(s) 342, 357, 628
BseNI ACTGG 3 cut(s) 392, 407, 682
BsePI GCGCGC 1 cut(s) 651
BseRI GAGGAG 1 cut(s) 356
BseXI GCAGC 4 cut(s) 39, 142, 215, 732
Bsh1236I CGCG 2 cut(s) 577, 653
BshFI GGCC 2 cut(s) 392, 632
BshTI ACCGGT 2 cut(s) 266, 543
BshVI ATCGAT 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 297
BslI CCNNNNNNNGG 3 cut(s) 342, 357, 628
BsmAI GTCTC 2 cut(s) 386, 557
BsmBI CGTCTC 1 cut(s) 557
BsnI GGCC 2 cut(s) 392, 632
Bso31I GGTCTC 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 539
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 2 cut(s) 315, 508
Bsp143I GATC 4 cut(s) 285, 318, 756, 760
Bsp19I CCATGG 1 cut(s) 609
BspANI GGCC 2 cut(s) 392, 632
BspDI ATCGAT 1 cut(s) 759
BspEI TCCGGA 2 cut(s) 315, 508
BspFNI CGCG 2 cut(s) 577, 653
BspLI GGNNCC 1 cut(s) 362
BspMAI CTGCAG 1 cut(s) 722
BspPI GGATC 2 cut(s) 293, 326
BspQI GCTCTTC 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 3 cut(s) 266, 350, 543
BsrI ACTGG 3 cut(s) 392, 407, 682
BssAI RCCGGY 3 cut(s) 266, 350, 543
BssECI CCNNGG 2 cut(s) 609, 633
BssHII GCGCGC 1 cut(s) 651
BssMI GATC 4 cut(s) 285, 318, 756, 760
BssNI GRCGYC 2 cut(s) 333, 737
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 3 cut(s) 308, 343, 416
Bst4CI ACNGT 4 cut(s) 47, 153, 196, 517
Bst6I CTCTTC 3 cut(s) 245, 374, 671
BstACI GRCGYC 2 cut(s) 333, 737
BstAPI GCANNNNNTGC 1 cut(s) 136
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 4 cut(s) 137, 348, 618, 653
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 2 cut(s) 10, 702
BstFNI CGCG 2 cut(s) 577, 653
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 4 cut(s) 135, 500, 653, 655
BstKTI GATC 4 cut(s) 288, 321, 759, 763
BstMAI GTCTC 2 cut(s) 386, 557
BstMBI GATC 4 cut(s) 285, 318, 756, 760
BstMWI GCNNNNNNNGC 2 cut(s) 89, 136
BstNI CCWGG 3 cut(s) 308, 343, 416
BstSCI CCNGG 4 cut(s) 306, 341, 414, 620
BstSFI CTRYAG 1 cut(s) 718
BstUI CGCG 2 cut(s) 577, 653
BstV1I GCAGC 4 cut(s) 39, 142, 215, 732
BstV2I GAAGAC 2 cut(s) 450, 495
Bsu15I ATCGAT 1 cut(s) 759
BsuRI GGCC 2 cut(s) 392, 632
BsuTUI ATCGAT 1 cut(s) 759
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 2 cut(s) 10, 702
Cac8I GCNNGC 4 cut(s) 137, 348, 618, 653
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 4 cut(s) 135, 500, 653, 655
Cfr10I RCCGGY 3 cut(s) 266, 350, 543
Cfr13I GGNCC 4 cut(s) 300, 361, 390, 546
ClaI ATCGAT 1 cut(s) 759
CpoI CGGWCCG 1 cut(s) 546
CseI GACGC 2 cut(s) 341, 745
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 2 cut(s) 169, 568
CspAI ACCGGT 2 cut(s) 266, 543
CspI CGGWCCG 1 cut(s) 546
CviAII CATG 5 cut(s) 61, 429, 610, 643, 754
CviQI GTAC 2 cut(s) 169, 568
DpnI GATC 4 cut(s) 287, 320, 758, 762
DpnII GATC 4 cut(s) 285, 318, 756, 760
Eam1104I CTCTTC 3 cut(s) 245, 374, 671
EarI CTCTTC 3 cut(s) 245, 374, 671
EciI GGCGGA 2 cut(s) 98, 100
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 3 cut(s) 300, 361, 546
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 306, 341, 414
EcoT14I CCWWGG 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 5 cut(s) 64, 432, 613, 646, 757
FaiI YATR 8 cut(s) 56, 62, 186, 224, 430, 611, 644, 755
FatI CATG 5 cut(s) 60, 428, 609, 642, 753
FauI CCCGC 2 cut(s) 539, 603
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 7 cut(s) 28, 131, 204, 336, 496, 575, 721
FokI GGATG 2 cut(s) 17, 689
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 7 cut(s) 28, 131, 204, 336, 496, 575, 721
FspBI CTAG 3 cut(s) 24, 402, 554
GlaI GCGC 4 cut(s) 134, 499, 652, 654
GluI GCNGC 7 cut(s) 28, 131, 204, 336, 496, 575, 721
GsuI CTGGAG 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 2 cut(s) 392, 632
HgaI GACGC 2 cut(s) 341, 745
HhaI GCGC 4 cut(s) 135, 500, 653, 655
Hin1I GRCGYC 2 cut(s) 333, 737
Hin1II CATG 5 cut(s) 64, 432, 613, 646, 757
Hin6I GCGC 4 cut(s) 133, 498, 651, 653
HinP1I GCGC 4 cut(s) 133, 498, 651, 653
HincII GTYRAC 3 cut(s) 199, 421, 520
HindII GTYRAC 3 cut(s) 199, 421, 520
HinfI GANTC 5 cut(s) 101, 467, 582, 659, 681
HpaI GTTAAC 1 cut(s) 520
HphI GGTGA 2 cut(s) 256, 577
Hpy166II GTNNAC 5 cut(s) 199, 412, 421, 520, 715
Hpy188I TCNGA 1 cut(s) 628
Hpy188III TCNNGA 7 cut(s) 272, 298, 316, 402, 509, 526, 712
Hpy8I GTNNAC 5 cut(s) 199, 412, 421, 520, 715
Hpy99I CGWCG 4 cut(s) 335, 563, 739, 745
HpyCH4III ACNGT 4 cut(s) 47, 153, 196, 517
HpyCH4IV ACGT 2 cut(s) 171, 566
HpyCH4V TGCA 1 cut(s) 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 136
HpySE526I ACGT 2 cut(s) 171, 566
Hsp92I GRCGYC 2 cut(s) 333, 737
Hsp92II CATG 5 cut(s) 64, 432, 613, 646, 757
HspAI GCGC 4 cut(s) 133, 498, 651, 653
Kpn2I TCCGGA 2 cut(s) 315, 508
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 4 cut(s) 285, 318, 756, 760
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 3 cut(s) 274, 511, 613
Lsp1109I GCAGC 4 cut(s) 39, 142, 215, 732
LweI GCATC 1 cut(s) 336
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 3 cut(s) 24, 402, 554
MaeII ACGT 2 cut(s) 171, 566
MalI GATC 4 cut(s) 287, 320, 758, 762
MbiI CCGCTC 1 cut(s) 238
MboI GATC 4 cut(s) 285, 318, 756, 760
MboII GAAGA 7 cut(s) 232, 361, 364, 367, 455, 500, 658
MhlI GDGCHC 1 cut(s) 297
MlyI GAGTC 3 cut(s) 95, 476, 653
MmeI TCCRAC 1 cut(s) 615
MnlI CCTC 7 cut(s) 118, 374, 377, 598, 622, 628, 716
MroI TCCGGA 2 cut(s) 315, 508
MseI TTAA 3 cut(s) 254, 519, 790
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 2 cut(s) 495, 604
MspR9I CCNGG 4 cut(s) 308, 343, 416, 622
MvaI CCWGG 3 cut(s) 308, 343, 416
MvnI CGCG 2 cut(s) 577, 653
MwoI GCNNNNNNNGC 2 cut(s) 89, 136
NciI CCSGG 1 cut(s) 622
NcoI CCATGG 1 cut(s) 609
NdeII GATC 4 cut(s) 285, 318, 756, 760
NlaIII CATG 5 cut(s) 64, 432, 613, 646, 757
NlaIV GGNNCC 1 cut(s) 362
NmeAIII GCCGAG 2 cut(s) 159, 658
NspV TTCGAA 1 cut(s) 539
PauI GCGCGC 1 cut(s) 651
PciSI GCTCTTC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 2 cut(s) 327, 654
PfeI GAWTC 2 cut(s) 582, 681
PflFI GACNNNGTC 1 cut(s) 739
PinAI ACCGGT 2 cut(s) 266, 543
PkrI GCNGC 7 cut(s) 29, 132, 205, 337, 497, 576, 722
PleI GAGTC 3 cut(s) 95, 475, 653
PpsI GAGTC 3 cut(s) 95, 475, 653
PpuMI RGGWCCY 1 cut(s) 361
PshBI ATTAAT 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 297
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 306, 341, 414
PspGI CCWGG 3 cut(s) 306, 341, 414
PspN4I GGNNCC 1 cut(s) 362
PspPI GGNCC 4 cut(s) 300, 361, 390, 546
PspPPI RGGWCCY 1 cut(s) 361
PstI CTGCAG 1 cut(s) 722
PstNI CAGNNNCTG 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 739
PteI GCGCGC 1 cut(s) 651
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 2 cut(s) 170, 569
RsaNI GTAC 2 cut(s) 169, 568
RseI CAYNNNNRTG 1 cut(s) 59
Rsr2I CGGWCCG 1 cut(s) 546
RsrII CGGWCCG 1 cut(s) 546
SacI GAGCTC 1 cut(s) 297
SalI GTCGAC 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 254, 519, 790
SatI GCNGC 7 cut(s) 28, 131, 204, 336, 496, 575, 721
Sau3AI GATC 4 cut(s) 285, 318, 756, 760
Sau96I GGNCC 4 cut(s) 300, 361, 390, 546
SchI GAGTC 3 cut(s) 95, 476, 653
ScrFI CCNGG 4 cut(s) 308, 343, 416, 622
SduI GDGCHC 1 cut(s) 297
SexAI ACCWGGT 1 cut(s) 414
SfaNI GCATC 1 cut(s) 336
SfcI CTRYAG 1 cut(s) 718
SfuI TTCGAA 1 cut(s) 539
SinI GGWCC 3 cut(s) 300, 361, 546
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SspMI CTAG 3 cut(s) 24, 402, 554
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 4 cut(s) 306, 341, 414, 620
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 4 cut(s) 47, 153, 196, 517
TaiI ACGT 2 cut(s) 174, 569
TaqI TCGA 7 cut(s) 321, 330, 420, 539, 657, 699, 759
TaqII GACCGA 1 cut(s) 368
TauI GCSGC 3 cut(s) 338, 498, 577
TfiI GAWTC 2 cut(s) 582, 681
Tru1I TTAA 3 cut(s) 254, 519, 790
Tru9I TTAA 3 cut(s) 254, 519, 790
TseI GCWGC 4 cut(s) 27, 130, 203, 720
TspDTI ATGAA 2 cut(s) 211, 232
TspGWI ACGGA 2 cut(s) 298, 451
Tth111I GACNNNGTC 1 cut(s) 739
VpaK11BI GGWCC 3 cut(s) 300, 361, 546
VspI ATTAAT 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 3 cut(s) 24, 402, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.