Prupe.4G012600_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014397
Physical Location & Seq
Reverse (-)
595584 .. 598721
3138 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G012600.1

Sequence Viewer

Length: 1125 bp
ATGGCGGCCTCATGTTTCCATGCACTTGGTCTTCGAAAGTCGAAGAGCAAGTCTTTACCAAACCCTTCCTCATCGAGACCCCAGTTCAATTCTGACATGGACAGCATGGAGAGGAGGAGATTTGATAGCTTGGACTCATGGTCTATGATATTGGAGTCGGAAAATGTGGAAACTTGGGAGGTGTCAAAGGAGGACCAGGAGGAGTGGACTGCTGATCTTTCTCAGCTGTTTATTGGCAACAAGTTCGCGTCTGGAGCACATAGTCGGATTTATCGTGGAATCTACAAGCAGAGAGCTGTTGCTGTGAAAATGGTGAGAATTCCAACCCAGAGGGAGGAGACCAGAACCTTACTTGAGCAGCAATTTAAGTCTGAAGTTGCCCTGCTTTCACGTCTCTTTCATCCCAACATAGTGCAGTTTATTGCAGCTTGTAAAAAGCCCCCTGTGTACTGTATCATCACGGAATATATGTCACAAGGAACGCTGAGGATGTATCTCAACAAGAAGGAGCCATACTCTCTTTCAACAGAAACAATACTGAGGTTAGCTCTTGACATATCCCGAGGAATGGAGTATCTTCATTCTCAAGGGGTGATCCACAGGGACCTCAAATCCAATAACTTGCTTCTTAATGATGATATGAGGGTTAAGGTGGCAGATTTTGGTACATCGTGTCTTGAAACACAGTGCCGGGAAACCAAGGGAAACAAGGGAACTTACCGTTGGATGGCACCGGAGATGATCAAGGAGAAACCTTGTACTCGGAAGGTTGATGTTTATAGTTTCGGGATTGTGTTATGGGAGCTGACAACAGCTTTGCTTCCCTTTCAAGGAATGACCCCTGTGCAGGCTGCATTTGCTGTGGCTGAAAAGAATGAACGCCCTCCAATGCCGGCTAGTTGTCAACCGGCGCTTGCGCACCTGATCAAGCGCTGTTGGGCAGCAAACCCTTCGAAGCGGCCGGATTTTAGCGACATTGTGTCTGCTTTGGAGAAGTATGATGAGTGTGTGAAGGAGGGCCTTCCTCTGACGCACCATTCCGGGCTGGTGAGCAGAAATGTCATTATTGAACGCTTGAAGGGCTGTGTATCCATGAGCTCTTCTTCTGTACCTGTACAAGCCTAA

Protein Analysis

375

Amino Acids

42.42

Weight (kDa)

8.9

Isoelectric Point (pI)

52.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62400
fragaria_vesca FvH4_3g01140
malus_domestica MD05G1356400.v1.1 MD10G1329500.v1.1
prunus_persica Prupe.4G012600_v2.0.a1
pyrus_communis pycom10g27930
rosa_chinensis RchiOBHm_Chr5g0001701
rosa_laevigata RLG00000030960
rosa_multiflora Rmu_ssc0000237.1_g000023
rosa_roxburghii Rroxscaffold_1G00071160 Rroxscaffold_1G00074620
rosa_rugosa Rorug04G0392000
rosa_samantha Rh5AG013400 Rh5BG016800 Rh5CG014400 Rh5DG014800
rosa_wichuraiana Rw5G001300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 918
AccB1I GGYRCC 1 cut(s) 730
AccII CGCG 1 cut(s) 248
AciI CCGC 2 cut(s) 5, 958
AclWI GGATC 1 cut(s) 589
AcoI YGGCCR 1 cut(s) 959
AcsI RAATTY 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 5 cut(s) 449, 667, 760, 1110, 1116
AfeI AGCGCT 1 cut(s) 932
AfiI CCNNNNNNNGG 5 cut(s) 334, 568, 727, 830, 847
AgsI TTSAA 6 cut(s) 88, 525, 680, 830, 1070, 1078
AhdI GACNNNNNGTC 2 cut(s) 139, 979
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AluBI AGCT 8 cut(s) 129, 226, 296, 428, 548, 805, 815, 1098
AluI AGCT 8 cut(s) 129, 226, 296, 428, 548, 805, 815, 1098
Alw21I GWGCWC 2 cut(s) 259, 1100
Alw26I GTCTC 3 cut(s) 70, 332, 398
AlwI GGATC 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 561
Aor51HI AGCGCT 1 cut(s) 932
AoxI GGCC 3 cut(s) 6, 959, 1018
ApeKI GCWGC 4 cut(s) 358, 425, 851, 941
ApoI RAATTY 1 cut(s) 318
ArsI GACNNNNNNTTYG 4 cut(s) 35, 67, 799, 831
AspLEI GCGC 3 cut(s) 913, 919, 933
AspS9I GGNCC 3 cut(s) 193, 604, 1018
AsuC2I CCSGG 2 cut(s) 692, 1042
AsuHPI GGTGA 3 cut(s) 325, 604, 1060
AsuII TTCGAA 2 cut(s) 34, 953
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 2 cut(s) 193, 604
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 1100
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 2 cut(s) 259, 1100
BbvCI CCTCAGC 1 cut(s) 485
BbvI GCAGC 4 cut(s) 370, 437, 838, 953
BccI CCATC 1 cut(s) 721
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 933, 967
BciT130I CCWGG 1 cut(s) 197
BciVI GTATCC 1 cut(s) 1099
BclI TGATCA 2 cut(s) 741, 924
BcnI CCSGG 2 cut(s) 692, 1042
BcoDI GTCTC 3 cut(s) 70, 332, 398
BfaI CTAG 1 cut(s) 897
BfoI RGCGCY 2 cut(s) 914, 934
BfuI GTATCC 1 cut(s) 1099
BisI GCNGC 6 cut(s) 6, 359, 426, 852, 942, 959
BlsI GCNGC 6 cut(s) 7, 360, 427, 853, 943, 960
Bme1390I CCNGG 3 cut(s) 197, 692, 1042
Bme18I GGWCC 2 cut(s) 193, 604
BmeRI GACNNNNNGTC 2 cut(s) 139, 979
BmeT110I CYCGRG 1 cut(s) 561
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 193, 604, 1018
BmiI GGNNCC 3 cut(s) 510, 605, 732
BmrFI CCNGG 3 cut(s) 197, 692, 1042
BmrI ACTGGG 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 23
BplI GAGNNNNNCTC 4 cut(s) 500, 532, 532, 564
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 485
Bpu14I TTCGAA 2 cut(s) 34, 953
BpuEI CTTGAG 2 cut(s) 374, 570
BpuMI CCSGG 2 cut(s) 692, 1042
BsaI GGTCTC 2 cut(s) 70, 332
BsaJI CCNNGG 2 cut(s) 562, 699
BsaWI WCCGGW 1 cut(s) 733
Bsc4I CCNNNNNNNGG 5 cut(s) 334, 568, 727, 830, 847
Bse118I RCCGGY 2 cut(s) 892, 907
Bse1I ACTGG 1 cut(s) 82
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 562, 699
BseGI GGATG 3 cut(s) 400, 495, 732
BseLI CCNNNNNNNGG 5 cut(s) 334, 568, 727, 830, 847
BseMII CTCAG 3 cut(s) 236, 476, 530
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 4 cut(s) 127, 130, 215, 350
BseX3I CGGCCG 1 cut(s) 959
BseXI GCAGC 4 cut(s) 370, 437, 838, 953
BsgI GTGCAG 2 cut(s) 434, 866
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 1 cut(s) 962
BshFI GGCC 3 cut(s) 8, 961, 1020
BshNI GGYRCC 1 cut(s) 730
BsiEI CGRYCG 1 cut(s) 962
BsiHKAI GWGCWC 2 cut(s) 259, 1100
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 6 cut(s) 691, 734, 893, 908, 962, 1041
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 5 cut(s) 334, 568, 727, 830, 847
BsmAI GTCTC 3 cut(s) 70, 332, 398
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 617
BsnI GGCC 3 cut(s) 8, 961, 1020
Bso31I GGTCTC 2 cut(s) 70, 332
BsoBI CYCGRG 1 cut(s) 561
Bsp119I TTCGAA 2 cut(s) 34, 953
Bsp1286I GDGCHC 2 cut(s) 259, 1100
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 4 cut(s) 214, 594, 741, 924
BspACI CCGC 2 cut(s) 5, 958
BspANI GGCC 3 cut(s) 8, 961, 1020
BspCNI CTCAG 3 cut(s) 235, 477, 531
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 510, 605, 732
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 2 cut(s) 38, 1105
BspT104I TTCGAA 2 cut(s) 34, 953
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 2 cut(s) 70, 332
BsrFI RCCGGY 2 cut(s) 892, 907
BsrGI TGTACA 1 cut(s) 1114
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 2 cut(s) 892, 907
BssECI CCNNGG 2 cut(s) 562, 699
BssMI GATC 4 cut(s) 214, 594, 741, 924
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 3 cut(s) 452, 687, 722
Bst6I CTCTTC 2 cut(s) 38, 1105
BstAUI TGTACA 1 cut(s) 1114
BstBI TTCGAA 2 cut(s) 34, 953
BstC8I GCNNGC 3 cut(s) 849, 894, 915
BstDEI CTNAG 3 cut(s) 222, 485, 539
BstF5I GGATG 3 cut(s) 400, 495, 732
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 2 cut(s) 914, 934
BstHHI GCGC 3 cut(s) 913, 919, 933
BstKTI GATC 4 cut(s) 217, 597, 744, 927
BstMAI GTCTC 3 cut(s) 70, 332, 398
BstMBI GATC 4 cut(s) 214, 594, 741, 924
BstMCI CGRYCG 1 cut(s) 962
BstMWI GCNNNNNNNGC 3 cut(s) 254, 857, 1080
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 195, 690, 1040
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 4 cut(s) 370, 437, 838, 953
BstV2I GAAGAC 1 cut(s) 23
BstXI CCANNNNNNTGG 1 cut(s) 26
BstZI CGGCCG 1 cut(s) 959
BsuI GTATCC 1 cut(s) 1099
BsuRI GGCC 3 cut(s) 8, 961, 1020
BtrI CACGTC 1 cut(s) 392
BtsCI GGATG 3 cut(s) 400, 495, 732
BtsIMutI CAGTG 1 cut(s) 692
Cac8I GCNNGC 3 cut(s) 849, 894, 915
CfoI GCGC 3 cut(s) 913, 919, 933
Cfr10I RCCGGY 2 cut(s) 892, 907
Cfr13I GGNCC 3 cut(s) 193, 604, 1018
CseI GACGC 2 cut(s) 237, 1039
Csp6I GTAC 5 cut(s) 448, 666, 759, 1109, 1115
CviAII CATG 6 cut(s) 12, 20, 97, 106, 138, 1093
CviQI GTAC 5 cut(s) 448, 666, 759, 1109, 1115
DdeI CTNAG 3 cut(s) 222, 485, 539
DpnI GATC 4 cut(s) 216, 596, 743, 926
DpnII GATC 4 cut(s) 214, 594, 741, 924
DriI GACNNNNNGTC 2 cut(s) 139, 979
EaeI YGGCCR 1 cut(s) 959
EagI CGGCCG 1 cut(s) 959
Eam1104I CTCTTC 2 cut(s) 38, 1105
Eam1105I GACNNNNNGTC 2 cut(s) 139, 979
EarI CTCTTC 2 cut(s) 38, 1105
Ecl136II GAGCTC 1 cut(s) 1098
EclXI CGGCCG 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 1 cut(s) 1100
Eco31I GGTCTC 2 cut(s) 70, 332
Eco47I GGWCC 2 cut(s) 193, 604
Eco47III AGCGCT 1 cut(s) 932
Eco52I CGGCCG 1 cut(s) 959
Eco53kI GAGCTC 1 cut(s) 1098
Eco57I CTGAAG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 561
EcoICRI GAGCTC 1 cut(s) 1098
EcoO109I RGGNCCY 2 cut(s) 604, 1018
EcoRI GAATTC 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 1100
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 398
FaeI CATG 6 cut(s) 15, 23, 100, 109, 141, 1096
FaqI GGGAC 1 cut(s) 617
FatI CATG 6 cut(s) 11, 19, 96, 105, 137, 1092
FbaI TGATCA 2 cut(s) 741, 924
Fnu4HI GCNGC 6 cut(s) 6, 359, 426, 852, 942, 959
FokI GGATG 3 cut(s) 387, 502, 739
FriOI GRGCYC 1 cut(s) 1100
Fsp4HI GCNGC 6 cut(s) 6, 359, 426, 852, 942, 959
FspBI CTAG 1 cut(s) 897
FspI TGCGCA 1 cut(s) 918
GlaI GCGC 3 cut(s) 912, 918, 932
GluI GCNGC 6 cut(s) 6, 359, 426, 852, 942, 959
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 2 cut(s) 914, 934
HaeIII GGCC 3 cut(s) 8, 961, 1020
HapII CCGG 6 cut(s) 691, 734, 893, 908, 962, 1041
HgaI GACGC 2 cut(s) 237, 1039
HhaI GCGC 3 cut(s) 913, 919, 933
Hin1II CATG 6 cut(s) 15, 23, 100, 109, 141, 1096
Hin6I GCGC 3 cut(s) 911, 917, 931
HinP1I GCGC 3 cut(s) 911, 917, 931
HincII GTYRAC 1 cut(s) 905
HindII GTYRAC 1 cut(s) 905
HinfI GANTC 3 cut(s) 134, 155, 279
HpaII CCGG 6 cut(s) 691, 734, 893, 908, 962, 1041
HphI GGTGA 3 cut(s) 325, 604, 1060
Hpy166II GTNNAC 3 cut(s) 207, 448, 905
Hpy188I TCNGA 6 cut(s) 94, 160, 267, 373, 765, 1029
Hpy188III TCNNGA 6 cut(s) 75, 252, 551, 561, 677, 787
Hpy8I GTNNAC 3 cut(s) 207, 448, 905
HpyAV CCTTC 7 cut(s) 75, 499, 760, 960, 1006, 1031, 1072
HpyCH4III ACNGT 3 cut(s) 452, 687, 722
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 5 cut(s) 23, 415, 425, 847, 854
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 857, 1080
HpyF3I CTNAG 3 cut(s) 222, 485, 539
HpySE526I ACGT 1 cut(s) 391
Hsp92II CATG 6 cut(s) 15, 23, 100, 109, 141, 1096
HspAI GCGC 3 cut(s) 911, 917, 931
KroI GCCGGC 1 cut(s) 892
KroNI GCCGGC 1 cut(s) 894
Ksp22I TGATCA 2 cut(s) 741, 924
Kzo9I GATC 4 cut(s) 214, 594, 741, 924
LguI GCTCTTC 2 cut(s) 38, 1105
LmnI GCTCC 3 cut(s) 254, 508, 802
Lsp1109I GCAGC 4 cut(s) 370, 437, 838, 953
MaeI CTAG 1 cut(s) 897
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 1 cut(s) 471
MalI GATC 4 cut(s) 216, 596, 743, 926
MboI GATC 4 cut(s) 214, 594, 741, 924
MboII GAAGA 5 cut(s) 23, 55, 569, 1092, 1095
MhlI GDGCHC 2 cut(s) 259, 1100
MluCI AATT 3 cut(s) 88, 318, 362
MlyI GAGTC 2 cut(s) 128, 164
MmeI TCCRAC 4 cut(s) 138, 245, 347, 704
MroNI GCCGGC 1 cut(s) 892
MseI TTAA 3 cut(s) 366, 630, 648
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 6 cut(s) 691, 734, 893, 908, 962, 1041
MspR9I CCNGG 3 cut(s) 197, 692, 1042
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 3 cut(s) 254, 857, 1080
NaeI GCCGGC 1 cut(s) 894
NciI CCSGG 2 cut(s) 692, 1042
NdeII GATC 4 cut(s) 214, 594, 741, 924
NgoMIV GCCGGC 1 cut(s) 892
NlaIII CATG 6 cut(s) 15, 23, 100, 109, 141, 1096
NlaIV GGNNCC 3 cut(s) 510, 605, 732
NmuCI GTSAC 1 cut(s) 471
NsbI TGCGCA 1 cut(s) 918
NspV TTCGAA 2 cut(s) 34, 953
PciSI GCTCTTC 2 cut(s) 38, 1105
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PdiI GCCGGC 1 cut(s) 894
PfeI GAWTC 1 cut(s) 279
PkrI GCNGC 6 cut(s) 7, 360, 427, 853, 943, 960
PleI GAGTC 2 cut(s) 128, 163
PpsI GAGTC 2 cut(s) 128, 163
PpuMI RGGWCCY 1 cut(s) 604
Psp124BI GAGCTC 1 cut(s) 1100
Psp5II RGGWCCY 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 3 cut(s) 510, 605, 732
PspPI GGNCC 3 cut(s) 193, 604, 1018
PspPPI RGGWCCY 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 226
RsaI GTAC 5 cut(s) 449, 667, 760, 1110, 1116
RsaNI GTAC 5 cut(s) 448, 666, 759, 1109, 1115
SacI GAGCTC 1 cut(s) 1100
SapI GCTCTTC 2 cut(s) 38, 1105
SaqAI TTAA 3 cut(s) 366, 630, 648
SatI GCNGC 6 cut(s) 6, 359, 426, 852, 942, 959
Sau3AI GATC 4 cut(s) 214, 594, 741, 924
Sau96I GGNCC 3 cut(s) 193, 604, 1018
SchI GAGTC 2 cut(s) 128, 164
ScrFI CCNGG 3 cut(s) 197, 692, 1042
SduI GDGCHC 2 cut(s) 259, 1100
SfuI TTCGAA 2 cut(s) 34, 953
SinI GGWCC 2 cut(s) 193, 604
SmlI CTYRAG 2 cut(s) 353, 585
SmoI CTYRAG 2 cut(s) 353, 585
Sse9I AATT 3 cut(s) 88, 318, 362
SsiI CCGC 2 cut(s) 5, 958
SspMI CTAG 1 cut(s) 897
SstI GAGCTC 1 cut(s) 1100
StyD4I CCNGG 3 cut(s) 195, 690, 1040
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 3 cut(s) 452, 687, 722
TaiI ACGT 1 cut(s) 394
TaqI TCGA 4 cut(s) 34, 41, 74, 953
TasI AATT 3 cut(s) 88, 318, 362
TatI WGTACW 3 cut(s) 447, 758, 1114
TauI GCSGC 2 cut(s) 8, 961
TfiI GAWTC 1 cut(s) 279
Tru1I TTAA 3 cut(s) 366, 630, 648
Tru9I TTAA 3 cut(s) 366, 630, 648
TscAI CASTG 1 cut(s) 692
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 4 cut(s) 358, 425, 851, 941
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 3 cut(s) 389, 569, 891
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 1 cut(s) 692
VpaK11BI GGWCC 2 cut(s) 193, 604
XapI RAATTY 1 cut(s) 318
XspI CTAG 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.