Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp04

Orthogroup ID

OG0013633

Physical Location & Seq

Genomic Coordinates Forward (+)

2979128 .. 2982751

Gene Structure 3624 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.4G062200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGGGTTCCCACAAAATTGAGCTGGCTTTTGCTTTCTTCATCTGGGCATCACTGGCATGCTTATCTTCTTGCCATAGCCTCCCAAGTGAATACTCCATAGTGGAGCAAAATGAAGTGCTCAACAACTTCCCTGCAGAAGAGAGAGTGGTGGAGCTCTTCAGGCTCTGGAAGCAGAAGCATGGAAAGGTGTATAGGCAAGCTGAAGAGAGTGAGAGGAGGTTTGAGAATTTCAAGAGGAACCTCAAGTTTGTATTGGAGAAGACTGCAAAGAAGAGGGCTGCAAACAATGCACATGATAGCCAGAGGGTGGGGTTGAATAGGTTTGCTGATATGAGCAATGAGGAGTTTAGGAAGACTTACTTGTCTAAAAAGCTGAAAATGCCCACCAACAAAAGGAACAGTATGATGAGGAGAATGCATGAAGAACCAGTGCATTCATGTGAGGCTCCTTCAGCTTTGGATTGGAGGAAAAAGGGAGCTGTGACTGGTGTCAAGGACCAAGGCAGTTGTGGAAGTTGCTGGGCATTCTCTACCACCGGAGCTATTGAAGGAATCAATGCAATAGCCACCGGAGAACTCATTAGTCTTTCAGAACAAGAACTTGTGGATTGTGACGGTACCAACGAGGGATGTGATGGAGGCTACATGGACTATGCTTTTGAATGGGTTATTGACAATGGTGGTATCGATACAGAAAAAAATTATCCATACACAGGTGTGGATGGTACCTGCAATGTCACCAAGGAGGAAACTAAAGTTGTCACCATTGATGGTTATGAAGATGTGGGAGAAACAGACGGTGATCTTTTATGTGCTGCCGTTCAGCAACCCTTTAGTGTTGGTATAGATGGTTCTGCGTGGGATTTCCAACTATACACAGGAGGAATCTACGATGGCGACTGCTCCGACAATCCAGATGACATTGACCATGCACCTCTGGTCGTAGGATATGGCTCTGAAGGCGATGAAGATTACTGGATTGTAAAGAATTCGTGGGGGACTAGTTGGGGGATGGATGGATACATTTACATAAGAAGGAACACTAATTTAAAATATGGGGTTTGTGCAATCAATGCCATGGCTTCATATCCTACCAAGGAGTCATCTGCACCATCCCCAACAGCTCCACCACCACCTCCTACACCAGTGTCACCACCCCCTCCACCAACACCACCTACACCAGTGACACCACCGCCACCTCCCTCTCCTTCACCAAGTGACTGTGGAGACTTCTCCTATTGTCCAAGTGATGAGACATGCTGTTGCCTCTTTGAATTCTTGGACTACTGCCTCATCTATGGTTGCTGTGAATATCAGAATGCGGTTTGCTGCACCGGAACTGACTACTGCTGCCCAAGTGATTATCCCATTTGTGATGTTGAAGATGGTCTCTGCCTCAAGAATGCGGGAGATTTCTGGGGAGTGTCTGCAAAGAAGAGAAAAATGGCCAAGCACAAGCTCCCATGGACTAAAGTAGAGCAAACAGAGAAGACATACCACCCACTGCAGTGGAAGATGAACCGGTTTGCTGCAATGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

514

Amino Acids

57.12

Weight (kDa)

4.95

Isoelectric Point (pI)

50.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g07700
malus_domestica	MD05G1300100.v1.1
prunus_persica	Prupe.4G062200_v2.0.a1 Prupe.4G062200_v2.0.a1 Prupe.4G062200_v2.0.a1
pyrus_communis	pycom05g27800 pycom05g27830
rosa_chinensis	RchiOBHm_Chr5g0010931
rosa_laevigata	RLG00000031798
rosa_multiflora	Rmu_sc0001895.1_g000004
rosa_roxburghii	Rroxscaffold_1G00065440
rosa_rugosa	Rorug04G0453800
rosa_samantha	Rh5AG086400 Rh5BG080900 Rh5CG093400 Rh5DG081000
rosa_wichuraiana	Rw0G017430 Rw0G017880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	361
Acc36I	ACCTGC	1 cut(s)	737
Acc65I	GGTACC	2 cut(s)	617, 725
AccB1I	GGYRCC	2 cut(s)	617, 725
AccB7I	CCANNNNNTGG	1 cut(s)	307
AciI	CCGC	3 cut(s)	1193, 1322, 1406
AcoI	YGGCCR	1 cut(s)	1446
AcsI	RAATTY	3 cut(s)	226, 988, 1274
AcuI	CTGAAG	4 cut(s)	142, 222, 435, 978
AdeI	CACNNNGTG	1 cut(s)	1217
AfaI	GTAC	2 cut(s)	619, 727
AfiI	CCNNNNNNNGG	3 cut(s)	307, 393, 713
AgeI	ACCGGT	1 cut(s)	1521
AgsI	TTSAA	6 cut(s)	232, 316, 548, 662, 1274, 1382
AhlI	ACTAGT	1 cut(s)	1001
AleI	CACNNNNGTG	2 cut(s)	716, 1507
AluBI	AGCT	9 cut(s)	22, 154, 200, 373, 455, 479, 542, 1124, 1459
AluI	AGCT	9 cut(s)	22, 154, 200, 373, 455, 479, 542, 1124, 1459
Alw21I	GWGCWC	2 cut(s)	120, 156
Alw26I	GTCTC	3 cut(s)	1221, 1247, 1394
AlwNI	CAGNNNCTG	1 cut(s)	165
AoxI	GGCC	1 cut(s)	1446
ApeKI	GCWGC	5 cut(s)	278, 815, 1329, 1350, 1529
ApoI	RAATTY	3 cut(s)	226, 988, 1274
ArsI	GACNNNNNNTTYG	2 cut(s)	641, 673
AsiGI	ACCGGT	1 cut(s)	1521
Asp718I	GGTACC	2 cut(s)	617, 725
AspLEI	GCGC	1 cut(s)	1539
AspS9I	GGNCC	1 cut(s)	496
AsuHPI	GGTGA	5 cut(s)	730, 754, 812, 1143, 1203
AvaII	GGWCC	1 cut(s)	496
BalI	TGGCCA	1 cut(s)	1448
BanI	GGYRCC	2 cut(s)	617, 725
BanII	GRGCYC	1 cut(s)	156
BbsI	GAAGAC	3 cut(s)	266, 359, 1496
Bbv12I	GWGCWC	2 cut(s)	120, 156
BbvI	GCAGC	5 cut(s)	265, 802, 1316, 1337, 1516
BccI	CCATC	9 cut(s)	629, 716, 764, 842, 887, 1006, 1010, 1120, 1379
BceAI	ACGGC	1 cut(s)	803
BciVI	GTATCC	1 cut(s)	1013
BcoDI	GTCTC	3 cut(s)	1221, 1247, 1394
BcuI	ACTAGT	1 cut(s)	1001
BfaI	CTAG	1 cut(s)	1002
BfmI	CTRYAG	2 cut(s)	132, 1505
BfuAI	ACCTGC	1 cut(s)	737
BfuI	GTATCC	1 cut(s)	1013
BisI	GCNGC	5 cut(s)	279, 816, 1330, 1351, 1530
BlsI	GCNGC	5 cut(s)	280, 817, 1331, 1352, 1531
Bme18I	GGWCC	1 cut(s)	496
BmgT120I	GGNCC	1 cut(s)	496
BmiI	GGNNCC	5 cut(s)	7, 239, 447, 619, 727
BmsI	GCATC	1 cut(s)	56
BoxI	GACNNNNGTC	1 cut(s)	488
BpiI	GAAGAC	3 cut(s)	266, 359, 1496
BpuEI	CTTGAG	2 cut(s)	227, 1382
Bsa29I	ATCGAT	1 cut(s)	687
BsaI	GGTCTC	1 cut(s)	1394
BsaJI	CCNNGG	5 cut(s)	499, 741, 1077, 1095, 1463
BsaWI	WCCGGW	4 cut(s)	536, 569, 1334, 1521
Bsc4I	CCNNNNNNNGG	3 cut(s)	307, 393, 713
Bse118I	RCCGGY	1 cut(s)	1521
Bse1I	ACTGG	6 cut(s)	57, 428, 490, 980, 1145, 1181
Bse3DI	GCAATG	3 cut(s)	343, 739, 1539
BseCI	ATCGAT	1 cut(s)	687
BseDI	CCNNGG	5 cut(s)	499, 741, 1077, 1095, 1463
BseGI	GGATG	5 cut(s)	635, 727, 1017, 1021, 1112
BseLI	CCNNNNNNNGG	3 cut(s)	307, 393, 713
BseMI	GCAATG	3 cut(s)	343, 739, 1539
BseNI	ACTGG	6 cut(s)	57, 428, 490, 980, 1145, 1181
BseRI	GAGGAG	3 cut(s)	229, 356, 424
BseXI	GCAGC	5 cut(s)	265, 802, 1316, 1337, 1516
BseYI	CCCAGC	1 cut(s)	519
BsgI	GTGCAG	2 cut(s)	1092, 1315
BshFI	GGCC	1 cut(s)	1448
BshNI	GGYRCC	2 cut(s)	617, 725
BshTI	ACCGGT	1 cut(s)	1521
BshVI	ATCGAT	1 cut(s)	687
BsiHKAI	GWGCWC	2 cut(s)	120, 156
BsiSI	CCGG	4 cut(s)	537, 570, 1335, 1522
BslFI	GGGAC	1 cut(s)	1012
BslI	CCNNNNNNNGG	3 cut(s)	307, 393, 713
BsmAI	GTCTC	3 cut(s)	1221, 1247, 1394
BsmFI	GGGAC	1 cut(s)	1012
BsmI	GAATGC	5 cut(s)	420, 433, 524, 1324, 1408
BsnI	GGCC	1 cut(s)	1448
Bso31I	GGTCTC	1 cut(s)	1394
Bsp1286I	GDGCHC	2 cut(s)	120, 156
Bsp143I	GATC	1 cut(s)	802
Bsp19I	CCATGG	2 cut(s)	1077, 1463
BspACI	CCGC	3 cut(s)	1193, 1322, 1406
BspANI	GGCC	1 cut(s)	1448
BspDI	ATCGAT	1 cut(s)	687
BspLI	GGNNCC	5 cut(s)	7, 239, 447, 619, 727
BspMAI	CTGCAG	2 cut(s)	136, 1509
BspMI	ACCTGC	1 cut(s)	737
BspQI	GCTCTTC	1 cut(s)	161
BspT107I	GGYRCC	2 cut(s)	617, 725
BspTNI	GGTCTC	1 cut(s)	1394
BsrDI	GCAATG	3 cut(s)	343, 739, 1539
BsrFI	RCCGGY	1 cut(s)	1521
BsrI	ACTGG	6 cut(s)	57, 428, 490, 980, 1145, 1181
BssAI	RCCGGY	1 cut(s)	1521
BssECI	CCNNGG	5 cut(s)	499, 741, 1077, 1095, 1463
BssMI	GATC	1 cut(s)	802
BssT1I	CCWWGG	5 cut(s)	499, 741, 1077, 1095, 1463
Bst4CI	ACNGT	4 cut(s)	401, 617, 800, 1223
Bst6I	CTCTTC	5 cut(s)	132, 161, 198, 266, 1430
BstAPI	GCANNNNNTGC	2 cut(s)	287, 1073
BstC8I	GCNNGC	3 cut(s)	24, 58, 198
BstDSI	CCRYGG	2 cut(s)	1077, 1463
BstF5I	GGATG	5 cut(s)	635, 727, 1017, 1021, 1112
BstHHI	GCGC	1 cut(s)	1539
BstKTI	GATC	1 cut(s)	805
BstMAI	GTCTC	3 cut(s)	1221, 1247, 1394
BstMBI	GATC	1 cut(s)	802
BstMWI	GCNNNNNNNGC	8 cut(s)	53, 160, 169, 287, 379, 452, 960, 1073
BstNSI	RCATGY	2 cut(s)	60, 1260
BstPAI	GACNNNNGTC	1 cut(s)	488
BstSFI	CTRYAG	2 cut(s)	132, 1505
BstV1I	GCAGC	5 cut(s)	265, 802, 1316, 1337, 1516
BstV2I	GAAGAC	3 cut(s)	266, 359, 1496
BstXI	CCANNNNNNTGG	1 cut(s)	1509
Bsu15I	ATCGAT	1 cut(s)	687
BsuI	GTATCC	1 cut(s)	1013
BsuRI	GGCC	1 cut(s)	1448
BsuTUI	ATCGAT	1 cut(s)	687
BtgI	CCRYGG	2 cut(s)	1077, 1463
BtgZI	GCGATG	1 cut(s)	978
BtsCI	GGATG	5 cut(s)	635, 727, 1017, 1021, 1112
BtsI	GCAGTG	2 cut(s)	1502, 1514
BtsIMutI	CAGTG	6 cut(s)	50, 435, 1152, 1188, 1502, 1514
BveI	ACCTGC	1 cut(s)	737
Cac8I	GCNNGC	3 cut(s)	24, 58, 198
CaiI	CAGNNNCTG	1 cut(s)	165
CfoI	GCGC	1 cut(s)	1539
Cfr10I	RCCGGY	1 cut(s)	1521
Cfr13I	GGNCC	1 cut(s)	496
ClaI	ATCGAT	1 cut(s)	687
Csp6I	GTAC	2 cut(s)	618, 726
CspAI	ACCGGT	1 cut(s)	1521
CviQI	GTAC	2 cut(s)	618, 726
DpnI	GATC	1 cut(s)	804
DpnII	GATC	1 cut(s)	802
DraI	TTTAAA	1 cut(s)	1050
DraIII	CACNNNGTG	1 cut(s)	1217
DrdI	GACNNNNNNGTC	1 cut(s)	361
DseDI	GACNNNNNNGTC	1 cut(s)	361
EaeI	YGGCCR	1 cut(s)	1446
Eam1104I	CTCTTC	5 cut(s)	132, 161, 198, 266, 1430
EarI	CTCTTC	5 cut(s)	132, 161, 198, 266, 1430
Ecl136II	GAGCTC	1 cut(s)	154
Eco130I	CCWWGG	5 cut(s)	499, 741, 1077, 1095, 1463
Eco24I	GRGCYC	1 cut(s)	156
Eco31I	GGTCTC	1 cut(s)	1394
Eco47I	GGWCC	1 cut(s)	496
Eco53kI	GAGCTC	1 cut(s)	154
Eco57I	CTGAAG	4 cut(s)	142, 222, 435, 978
EcoICRI	GAGCTC	1 cut(s)	154
EcoRI	GAATTC	2 cut(s)	988, 1274
EcoT14I	CCWWGG	5 cut(s)	499, 741, 1077, 1095, 1463
EcoT22I	ATGCAT	1 cut(s)	420
EcoT38I	GRGCYC	1 cut(s)	156
ErhI	CCWWGG	5 cut(s)	499, 741, 1077, 1095, 1463
FalI	AAGNNNNNCTT	2 cut(s)	344, 376
FaqI	GGGAC	1 cut(s)	1012
FauI	CCCGC	1 cut(s)	1399
Fnu4HI	GCNGC	5 cut(s)	279, 816, 1330, 1351, 1530
FokI	GGATG	5 cut(s)	642, 734, 1024, 1028, 1099
FriOI	GRGCYC	1 cut(s)	156
Fsp4HI	GCNGC	5 cut(s)	279, 816, 1330, 1351, 1530
FspBI	CTAG	1 cut(s)	1002
GlaI	GCGC	1 cut(s)	1538
GluI	GCNGC	5 cut(s)	279, 816, 1330, 1351, 1530
GsaI	CCCAGC	1 cut(s)	523
HaeIII	GGCC	1 cut(s)	1448
HapII	CCGG	4 cut(s)	537, 570, 1335, 1522
HhaI	GCGC	1 cut(s)	1539
Hin6I	GCGC	1 cut(s)	1537
HinP1I	GCGC	1 cut(s)	1537
HinfI	GANTC	3 cut(s)	552, 885, 1100
HpaII	CCGG	4 cut(s)	537, 570, 1335, 1522
HphI	GGTGA	5 cut(s)	730, 754, 812, 1143, 1203
Hpy188I	TCNGA	4 cut(s)	592, 907, 958, 1317
Hpy188III	TCNNGA	4 cut(s)	166, 232, 914, 1399
HpyAV	CCTTC	5 cut(s)	459, 542, 953, 1029, 1218
HpyCH4III	ACNGT	4 cut(s)	401, 617, 800, 1223
HpyF10VI	GCNNNNNNNGC	8 cut(s)	53, 160, 169, 287, 379, 452, 960, 1073
HspAI	GCGC	1 cut(s)	1537
KpnI	GGTACC	2 cut(s)	621, 729
Kzo9I	GATC	1 cut(s)	802
LguI	GCTCTTC	1 cut(s)	161
LmnI	GCTCC	8 cut(s)	103, 151, 451, 476, 539, 908, 1129, 1464
Lsp1109I	GCAGC	5 cut(s)	265, 802, 1316, 1337, 1516
LweI	GCATC	1 cut(s)	56
MaeI	CTAG	1 cut(s)	1002
MaeIII	GTNAC	7 cut(s)	481, 611, 736, 760, 1149, 1183, 1217
MalI	GATC	1 cut(s)	804
MboI	GATC	1 cut(s)	802
MhlI	GDGCHC	2 cut(s)	120, 156
MlsI	TGGCCA	1 cut(s)	1448
MluCI	AATT	6 cut(s)	15, 226, 700, 988, 1045, 1274
MluNI	TGGCCA	1 cut(s)	1448
MlyI	GAGTC	1 cut(s)	1109
MmeI	TCCRAC	2 cut(s)	892, 930
Mox20I	TGGCCA	1 cut(s)	1448
Mph1103I	ATGCAT	1 cut(s)	420
MscI	TGGCCA	1 cut(s)	1448
MseI	TTAA	1 cut(s)	1049
MslI	CAYNNNNRTG	4 cut(s)	55, 438, 716, 1507
Msp20I	TGGCCA	1 cut(s)	1448
MspI	CCGG	4 cut(s)	537, 570, 1335, 1522
Mva1269I	GAATGC	5 cut(s)	420, 433, 524, 1324, 1408
MwoI	GCNNNNNNNGC	8 cut(s)	53, 160, 169, 287, 379, 452, 960, 1073
NcoI	CCATGG	2 cut(s)	1077, 1463
NdeII	GATC	1 cut(s)	802
NlaIV	GGNNCC	5 cut(s)	7, 239, 447, 619, 727
NmuCI	GTSAC	7 cut(s)	481, 611, 736, 760, 1149, 1183, 1217
NsiI	ATGCAT	1 cut(s)	420
NspI	RCATGY	2 cut(s)	60, 1260
OliI	CACNNNNGTG	2 cut(s)	716, 1507
PaeI	GCATGC	1 cut(s)	60
PciSI	GCTCTTC	1 cut(s)	161
PcsI	WCGNNNNNNNCGW	1 cut(s)	621
PctI	GAATGC	5 cut(s)	420, 433, 524, 1324, 1408
PfeI	GAWTC	2 cut(s)	552, 885
PflMI	CCANNNNNTGG	1 cut(s)	307
PinAI	ACCGGT	1 cut(s)	1521
PkrI	GCNGC	5 cut(s)	280, 817, 1331, 1352, 1531
PleI	GAGTC	1 cut(s)	1108
PpsI	GAGTC	1 cut(s)	1108
PshAI	GACNNNNGTC	1 cut(s)	488
Psp124BI	GAGCTC	1 cut(s)	156
PspFI	CCCAGC	1 cut(s)	519
PspN4I	GGNNCC	5 cut(s)	7, 239, 447, 619, 727
PspPI	GGNCC	1 cut(s)	496
PsrI	GAACNNNNNNTAC	2 cut(s)	835, 867
PstI	CTGCAG	2 cut(s)	136, 1509
PstNI	CAGNNNCTG	1 cut(s)	165
RsaI	GTAC	2 cut(s)	619, 727
RsaNI	GTAC	2 cut(s)	618, 726
RseI	CAYNNNNRTG	4 cut(s)	55, 438, 716, 1507
SacI	GAGCTC	1 cut(s)	156
SapI	GCTCTTC	1 cut(s)	161
SaqAI	TTAA	1 cut(s)	1049
SatI	GCNGC	5 cut(s)	279, 816, 1330, 1351, 1530
Sau3AI	GATC	1 cut(s)	802
Sau96I	GGNCC	1 cut(s)	496
SchI	GAGTC	1 cut(s)	1109
SduI	GDGCHC	2 cut(s)	120, 156
SfaNI	GCATC	1 cut(s)	56
SfcI	CTRYAG	2 cut(s)	132, 1505
SinI	GGWCC	1 cut(s)	496
SmiMI	CAYNNNNRTG	4 cut(s)	55, 438, 716, 1507
SmlI	CTYRAG	2 cut(s)	242, 1397
SmoI	CTYRAG	2 cut(s)	242, 1397
SpeI	ACTAGT	1 cut(s)	1001
SphI	GCATGC	1 cut(s)	60
Sse9I	AATT	6 cut(s)	15, 226, 700, 988, 1045, 1274
SsiI	CCGC	3 cut(s)	1193, 1322, 1406
SspMI	CTAG	1 cut(s)	1002
SstI	GAGCTC	1 cut(s)	156
StyI	CCWWGG	5 cut(s)	499, 741, 1077, 1095, 1463
TaaI	ACNGT	4 cut(s)	401, 617, 800, 1223
TaqI	TCGA	1 cut(s)	687
TasI	AATT	6 cut(s)	15, 226, 700, 988, 1045, 1274
TfiI	GAWTC	2 cut(s)	552, 885
Tru1I	TTAA	1 cut(s)	1049
Tru9I	TTAA	1 cut(s)	1049
TscAI	CASTG	6 cut(s)	57, 435, 1152, 1188, 1509, 1514
TseFI	GTSAC	7 cut(s)	481, 611, 736, 760, 1149, 1183, 1217
TseI	GCWGC	5 cut(s)	278, 815, 1329, 1350, 1529
Tsp45I	GTSAC	7 cut(s)	481, 611, 736, 760, 1149, 1183, 1217
TspDTI	ATGAA	8 cut(s)	28, 126, 426, 435, 792, 981, 1074, 1532
TspRI	CASTG	6 cut(s)	57, 435, 1152, 1188, 1509, 1514
Van91I	CCANNNNNTGG	1 cut(s)	307
VpaK11BI	GGWCC	1 cut(s)	496
XapI	RAATTY	3 cut(s)	226, 988, 1274
XceI	RCATGY	2 cut(s)	60, 1260
XcmI	CCANNNNNNNNNTGG	1 cut(s)	506
XspI	CTAG	1 cut(s)	1002
Zsp2I	ATGCAT	1 cut(s)	420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.