Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0013633

Physical Location & Seq

Genomic Coordinates Forward (+)

8296360 .. 8298977

Gene Structure 2618 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031798

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1533 bp

ATGGGTTGCCACAAAATTGAACTGGCTCTTGCTTTTTTCATCTGTGCTTCACTAGCTTGCTTCTCTTATGGTCTCCCTAGTGAGTACTCCATAGTGGGAGGTGAAACAGTAAACTTTCCAGCTGAGGAGAGAGTGGTGGAGCTCTTCAGGCTGTGGAGGGAGAAGCACAGAAAGGTGTATAACCATGTTGAGGAGCAAGAGAAGAGGTTTGAGAATTTCAAGAGGAATCTCAGGTTTGTGTTGGAGAAACATGCACAGAAAAAGGCTGCAGCTAATAAGCATGATACTCAGAGAGTTGGGTTGAACAAGTTTGCTGATTTGAGCAATGAGGAGTTCAGGGCGATTTATATGCCGAAAAAGGTCCGAATGCCGATCAGCAAAAGGGACAGACTGGTGAGGAAAATGCAGCAGCAAAAGAAAGCAGAGCTGCCTAAGGACGCTCCTGCAAGTTTCGATTGGAGGAAGGCGGGAATTGTCACTCCTGTTAAGGACCAAGGCAGTTGTGGAAGTTGTTGGGCATTTTCTTCCACTGGAGCCATCGAAGGAATAAATGCACTGGACACCGGAGACCTCATTAGTCTTTCGGAGCAAGAACTTGTGGATTGTGACACTACCAACGAAGGCTGCAGTGGAGGCTACATGGACTACGCTTTTGAATGGGTTATTAGCAATGGTGGCATTGACACAGAATCTGATTATGCTTACACAGGTGTTGATGGTACCTGCAATGTCACCAAGGAGGAAACCAAAGTTGTCACCATCGATGGCTATACAGATGTGGAAGAGACAGAGACCGGTGTTTTCAATGCGGTGCTTCAGCAACCCATTAGCGTTGGTATGGATGGCGCTGCTTTGGATTTTCAATTATACTCCGGAGGAATTTATGACGGTGATTGCTCCGACGATCCAAATGACATTGATCATGCCGTTCTAATAGTAGGATATGGTTCTGAAAACGGTGAAGATTACTGGATTGTAAAGAATTCATGGGGAACTAGTTGGGGGATAGAAGGATACTTTTACCTCAGAAGGAACACCGATTTGCCATATGGGGTTTGTGCAGTCAATGCCATGGCTTCATATCCTACCAAAGAGTCAGTTGCACCCTCTCCTTATCCAACCCCAACTCCTCCACCACCACCTCCTACACCTGTCTCTCCACCACCGCCACCAGTGACACCGCCACCACCACCCTCTCCTTCACCAAGCCAATGCGGAGACTTCTCCTATTGTCCAGCTGATGAGACATGTTGTTGCCTCTATGAATTCTTTGACTACTGCTTGATTTATGGTTGCTGTGCATATGAGAATGCGGTTTGCTGCACTGGAACTGAATACTGCTGTCCAGGTGACTATCCCATTTGTGATGTCGAGGAAGGCCTGTGTCTTAAGAATGGCAGAGACTACTTGGGAGTAGCTGCAAAGAAGAGAAAGATTGCCAAGCACAAGTTCCCATGGACTAAAGTAGAGCAAACCGAGAAGACATACCACCCTCTACAGTGGAAGATGAACCGGTTTGCAGCAATGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

511

Amino Acids

56.86

Weight (kDa)

5.04

Isoelectric Point (pI)

48.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	49 - 112	2.5e-13	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	148 - 363	6.6e-80	Papain family cysteine protease
Granulin	PF00396	416 - 463	1.9e-06	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g07700
malus_domestica	MD05G1300100.v1.1
prunus_persica	Prupe.4G062200_v2.0.a1 Prupe.4G062200_v2.0.a1 Prupe.4G062200_v2.0.a1
pyrus_communis	pycom05g27800 pycom05g27830
rosa_chinensis	RchiOBHm_Chr5g0010931
rosa_laevigata	RLG00000031798
rosa_multiflora	Rmu_sc0001895.1_g000004
rosa_roxburghii	Rroxscaffold_1G00065440
rosa_rugosa	Rorug04G0453800
rosa_samantha	Rh5AG086400 Rh5BG080900 Rh5CG093400 Rh5DG081000
rosa_wichuraiana	Rw0G017430 Rw0G017880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	731
Acc65I	GGTACC	1 cut(s)	719
AccB1I	GGYRCC	1 cut(s)	719
AccIII	TCCGGA	1 cut(s)	872
AciI	CCGC	6 cut(s)	467, 809, 1166, 1181, 1215, 1313
AclWI	GGATC	1 cut(s)	899
AcsI	RAATTY	4 cut(s)	214, 879, 982, 1265
AcuI	CTGAAG	2 cut(s)	130, 800
AfaI	GTAC	2 cut(s)	86, 721
AfiI	CCNNNNNNNGG	1 cut(s)	190
AflII	CTTAAG	1 cut(s)	1388
AflIII	ACRYGT	1 cut(s)	1247
AgeI	ACCGGT	2 cut(s)	794, 1512
AgsI	TTSAA	6 cut(s)	20, 220, 304, 656, 805, 863
AhlI	ACTAGT	1 cut(s)	995
AjnI	CCWGG	1 cut(s)	1345
AjuI	GAANNNNNNNTTGG	2 cut(s)	1433, 1465
AluBI	AGCT	7 cut(s)	56, 122, 142, 272, 427, 1238, 1418
AluI	AGCT	7 cut(s)	56, 122, 142, 272, 427, 1238, 1418
Alw21I	GWGCWC	1 cut(s)	144
Alw26I	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1212, 1238, 1395
AlwI	GGATC	1 cut(s)	899
AlwNI	CAGNNNCTG	1 cut(s)	692
Aor13HI	TCCGGA	1 cut(s)	872
AoxI	GGCC	1 cut(s)	1378
ApoI	RAATTY	4 cut(s)	214, 879, 982, 1265
ArsI	GACNNNNNNTTYG	2 cut(s)	635, 667
AsiGI	ACCGGT	2 cut(s)	794, 1512
Asp718I	GGTACC	1 cut(s)	719
AspLEI	GCGC	2 cut(s)	848, 1530
AspS9I	GGNCC	2 cut(s)	361, 490
AsuHPI	GGTGA	8 cut(s)	113, 406, 724, 748, 902, 971, 1194, 1361
AvaII	GGWCC	2 cut(s)	361, 490
AxyI	CCTNAGG	1 cut(s)	432
BanI	GGYRCC	1 cut(s)	719
BanII	GRGCYC	1 cut(s)	144
BbsI	GAAGAC	1 cut(s)	1487
Bbv12I	GWGCWC	1 cut(s)	144
BbvCI	CCTCAGC	1 cut(s)	123
BbvI	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 835, 1307, 1405
BccI	CCATC	5 cut(s)	545, 710, 758, 767, 836
BceAI	ACGGC	1 cut(s)	911
BcgI	CGANNNNNNTGC	2 cut(s)	331, 365
BciT130I	CCWGG	1 cut(s)	1347
BciVI	GTATCC	1 cut(s)	1007
BclI	TGATCA	1 cut(s)	919
BcoDI	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1212, 1238, 1395
BcuI	ACTAGT	1 cut(s)	995
BfaI	CTAG	3 cut(s)	53, 78, 996
BfmI	CTRYAG	3 cut(s)	267, 625, 1496
BfoI	RGCGCY	1 cut(s)	849
BfrI	CTTAAG	1 cut(s)	1388
BfuAI	ACCTGC	1 cut(s)	731
BfuI	GTATCC	1 cut(s)	1007
BmcAI	AGTACT	1 cut(s)	86
Bme1390I	CCNGG	1 cut(s)	1347
Bme18I	GGWCC	2 cut(s)	361, 490
BmgT120I	GGNCC	2 cut(s)	361, 490
BmiI	GGNNCC	2 cut(s)	535, 721
BmrFI	CCNGG	1 cut(s)	1347
BpiI	GAAGAC	1 cut(s)	1487
BpmI	CTGGAG	1 cut(s)	552
Bpu10I	CCTNAGC	1 cut(s)	123
Bsa29I	ATCGAT	1 cut(s)	762
BsaI	GGTCTC	3 cut(s)	77, 561, 785
BsaJI	CCNNGG	4 cut(s)	493, 735, 1071, 1454
BsaWI	WCCGGW	4 cut(s)	563, 794, 872, 1512
BsaXI	ACNNNNNCTCC	2 cut(s)	1111, 1141
Bsc4I	CCNNNNNNNGG	1 cut(s)	190
Bse118I	RCCGGY	2 cut(s)	794, 1512
Bse1I	ACTGG	7 cut(s)	27, 396, 535, 561, 974, 1172, 1330
Bse21I	CCTNAGG	1 cut(s)	432
Bse3DI	GCAATG	4 cut(s)	331, 676, 733, 1530
BseAI	TCCGGA	1 cut(s)	872
BseBI	CCWGG	1 cut(s)	1347
BseCI	ATCGAT	1 cut(s)	762
BseDI	CCNNGG	4 cut(s)	493, 735, 1071, 1454
BseGI	GGATG	1 cut(s)	847
BseLI	CCNNNNNNNGG	1 cut(s)	190
BseMI	GCAATG	4 cut(s)	331, 676, 733, 1530
BseMII	CTCAG	4 cut(s)	114, 244, 302, 1039
BseNI	ACTGG	7 cut(s)	27, 396, 535, 561, 974, 1172, 1330
BseRI	GAGGAG	4 cut(s)	140, 206, 344, 1119
BseXI	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 835, 1307, 1405
BsgI	GTGCAG	2 cut(s)	1080, 1306
BshFI	GGCC	1 cut(s)	1380
BshNI	GGYRCC	1 cut(s)	719
BshTI	ACCGGT	2 cut(s)	794, 1512
BshVI	ATCGAT	1 cut(s)	762
BsiHKAI	GWGCWC	1 cut(s)	144
BsiSI	CCGG	4 cut(s)	564, 795, 873, 1513
BslFI	GGGAC	1 cut(s)	398
BslI	CCNNNNNNNGG	1 cut(s)	190
BsmAI	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1212, 1238, 1395
BsmFI	GGGAC	1 cut(s)	398
BsmI	GAATGC	2 cut(s)	372, 1315
BsnI	GGCC	1 cut(s)	1380
Bso31I	GGTCTC	3 cut(s)	77, 561, 785
Bsp1286I	GDGCHC	1 cut(s)	144
Bsp13I	TCCGGA	1 cut(s)	872
Bsp143I	GATC	3 cut(s)	372, 904, 919
Bsp19I	CCATGG	2 cut(s)	1071, 1454
BspACI	CCGC	6 cut(s)	467, 809, 1166, 1181, 1215, 1313
BspANI	GGCC	1 cut(s)	1380
BspCNI	CTCAG	4 cut(s)	115, 243, 301, 1038
BspDI	ATCGAT	1 cut(s)	762
BspEI	TCCGGA	1 cut(s)	872
BspLI	GGNNCC	2 cut(s)	535, 721
BspMAI	CTGCAG	2 cut(s)	271, 629
BspMI	ACCTGC	1 cut(s)	731
BspPI	GGATC	1 cut(s)	899
BspQI	GCTCTTC	1 cut(s)	149
BspT107I	GGYRCC	1 cut(s)	719
BspTI	CTTAAG	1 cut(s)	1388
BspTNI	GGTCTC	3 cut(s)	77, 561, 785
BsrDI	GCAATG	4 cut(s)	331, 676, 733, 1530
BsrFI	RCCGGY	2 cut(s)	794, 1512
BsrI	ACTGG	7 cut(s)	27, 396, 535, 561, 974, 1172, 1330
BssAI	RCCGGY	2 cut(s)	794, 1512
BssECI	CCNNGG	4 cut(s)	493, 735, 1071, 1454
BssMI	GATC	3 cut(s)	372, 904, 919
BssT1I	CCWWGG	4 cut(s)	493, 735, 1071, 1454
Bst2UI	CCWGG	1 cut(s)	1347
Bst4CI	ACNGT	4 cut(s)	109, 890, 959, 1500
Bst6I	CTCTTC	4 cut(s)	149, 197, 777, 1421
BstAFI	CTTAAG	1 cut(s)	1388
BstAPI	GCANNNNNTGC	1 cut(s)	1067
BstC8I	GCNNGC	1 cut(s)	58
BstDEI	CTNAG	5 cut(s)	123, 230, 288, 432, 1025
BstDSI	CCRYGG	2 cut(s)	1071, 1454
BstF5I	GGATG	1 cut(s)	847
BstH2I	RGCGCY	1 cut(s)	849
BstHHI	GCGC	2 cut(s)	848, 1530
BstKTI	GATC	3 cut(s)	375, 907, 922
BstMAI	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1212, 1238, 1395
BstMBI	GATC	3 cut(s)	372, 904, 919
BstMWI	GCNNNNNNNGC	5 cut(s)	53, 148, 633, 675, 1067
BstNI	CCWGG	1 cut(s)	1347
BstNSI	RCATGY	2 cut(s)	254, 1251
BstSCI	CCNGG	1 cut(s)	1345
BstSFI	CTRYAG	3 cut(s)	267, 625, 1496
BstV1I	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 835, 1307, 1405
BstV2I	GAAGAC	1 cut(s)	1487
Bsu15I	ATCGAT	1 cut(s)	762
Bsu36I	CCTNAGG	1 cut(s)	432
BsuI	GTATCC	1 cut(s)	1007
BsuRI	GGCC	1 cut(s)	1380
BsuTUI	ATCGAT	1 cut(s)	762
BtgI	CCRYGG	2 cut(s)	1071, 1454
BtsCI	GGATG	1 cut(s)	847
BtsI	GCAGTG	1 cut(s)	634
BtsIMutI	CAGTG	6 cut(s)	528, 554, 634, 1179, 1323, 1505
BveI	ACCTGC	1 cut(s)	731
Cac8I	GCNNGC	1 cut(s)	58
CaiI	CAGNNNCTG	1 cut(s)	692
CfoI	GCGC	2 cut(s)	848, 1530
Cfr10I	RCCGGY	2 cut(s)	794, 1512
Cfr13I	GGNCC	2 cut(s)	361, 490
ClaI	ATCGAT	1 cut(s)	762
CseI	GACGC	1 cut(s)	446
Csp6I	GTAC	2 cut(s)	85, 720
CspAI	ACCGGT	2 cut(s)	794, 1512
CviAII	CATG	9 cut(s)	185, 251, 281, 640, 923, 987, 1072, 1248, 1455
CviQI	GTAC	2 cut(s)	85, 720
DdeI	CTNAG	5 cut(s)	123, 230, 288, 432, 1025
DpnI	GATC	3 cut(s)	374, 906, 921
DpnII	GATC	3 cut(s)	372, 904, 919
Eam1104I	CTCTTC	4 cut(s)	149, 197, 777, 1421
EarI	CTCTTC	4 cut(s)	149, 197, 777, 1421
Ecl136II	GAGCTC	1 cut(s)	142
Eco130I	CCWWGG	4 cut(s)	493, 735, 1071, 1454
Eco147I	AGGCCT	1 cut(s)	1380
Eco24I	GRGCYC	1 cut(s)	144
Eco31I	GGTCTC	3 cut(s)	77, 561, 785
Eco47I	GGWCC	2 cut(s)	361, 490
Eco53kI	GAGCTC	1 cut(s)	142
Eco57I	CTGAAG	2 cut(s)	130, 800
Eco81I	CCTNAGG	1 cut(s)	432
EcoICRI	GAGCTC	1 cut(s)	142
EcoRI	GAATTC	2 cut(s)	982, 1265
EcoRII	CCWGG	1 cut(s)	1345
EcoT14I	CCWWGG	4 cut(s)	493, 735, 1071, 1454
EcoT38I	GRGCYC	1 cut(s)	144
ErhI	CCWWGG	4 cut(s)	493, 735, 1071, 1454
FaeI	CATG	9 cut(s)	188, 254, 284, 643, 926, 990, 1075, 1251, 1458
FaqI	GGGAC	1 cut(s)	398
FatI	CATG	9 cut(s)	184, 250, 280, 639, 922, 986, 1071, 1247, 1454
FauI	CCCGC	1 cut(s)	460
FauNDI	CATATG	2 cut(s)	1048, 1303
FbaI	TGATCA	1 cut(s)	919
FokI	GGATG	1 cut(s)	854
FriOI	GRGCYC	1 cut(s)	144
FspBI	CTAG	3 cut(s)	53, 78, 996
GlaI	GCGC	2 cut(s)	847, 1529
GsuI	CTGGAG	1 cut(s)	552
HaeII	RGCGCY	1 cut(s)	849
HaeIII	GGCC	1 cut(s)	1380
HapII	CCGG	4 cut(s)	564, 795, 873, 1513
HgaI	GACGC	1 cut(s)	446
HhaI	GCGC	2 cut(s)	848, 1530
Hin1II	CATG	9 cut(s)	188, 254, 284, 643, 926, 990, 1075, 1251, 1458
Hin6I	GCGC	2 cut(s)	846, 1528
HinP1I	GCGC	2 cut(s)	846, 1528
HinfI	GANTC	3 cut(s)	226, 689, 1094
HpaII	CCGG	4 cut(s)	564, 795, 873, 1513
HphI	GGTGA	8 cut(s)	113, 406, 724, 748, 902, 971, 1194, 1361
Hpy166II	GTNNAC	1 cut(s)	112
Hpy188I	TCNGA	7 cut(s)	291, 365, 586, 694, 901, 952, 1028
Hpy188III	TCNNGA	2 cut(s)	220, 873
Hpy8I	GTNNAC	1 cut(s)	112
Hpy99I	CGWCG	1 cut(s)	905
HpyAV	CCTTC	7 cut(s)	457, 536, 614, 1004, 1023, 1209, 1370
HpyCH4III	ACNGT	4 cut(s)	109, 890, 959, 1500
HpyF10VI	GCNNNNNNNGC	5 cut(s)	53, 148, 633, 675, 1067
HpyF3I	CTNAG	5 cut(s)	123, 230, 288, 432, 1025
Hsp92II	CATG	9 cut(s)	188, 254, 284, 643, 926, 990, 1075, 1251, 1458
HspAI	GCGC	2 cut(s)	846, 1528
Kpn2I	TCCGGA	1 cut(s)	872
KpnI	GGTACC	1 cut(s)	723
Ksp22I	TGATCA	1 cut(s)	919
Kzo9I	GATC	3 cut(s)	372, 904, 919
LguI	GCTCTTC	1 cut(s)	149
LmnI	GCTCC	6 cut(s)	139, 193, 445, 533, 586, 902
Lsp1109I	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 835, 1307, 1405
MaeI	CTAG	3 cut(s)	53, 78, 996
MaeIII	GTNAC	6 cut(s)	475, 605, 730, 754, 1174, 1349
MalI	GATC	3 cut(s)	374, 906, 921
MboI	GATC	3 cut(s)	372, 904, 919
MboII	GAAGA	8 cut(s)	136, 214, 516, 794, 974, 1438, 1492, 1516
MhlI	GDGCHC	1 cut(s)	144
MluCI	AATT	7 cut(s)	15, 214, 471, 863, 879, 982, 1265
MlyI	GAGTC	1 cut(s)	1103
MmeI	TCCRAC	3 cut(s)	222, 924, 1142
MroI	TCCGGA	1 cut(s)	872
MseI	TTAA	2 cut(s)	486, 1389
MspA1I	CMGCKG	2 cut(s)	122, 1238
MspCI	CTTAAG	1 cut(s)	1388
MspI	CCGG	4 cut(s)	564, 795, 873, 1513
MspR9I	CCNGG	1 cut(s)	1347
Mva1269I	GAATGC	2 cut(s)	372, 1315
MvaI	CCWGG	1 cut(s)	1347
MwoI	GCNNNNNNNGC	5 cut(s)	53, 148, 633, 675, 1067
NcoI	CCATGG	2 cut(s)	1071, 1454
NdeI	CATATG	2 cut(s)	1048, 1303
NdeII	GATC	3 cut(s)	372, 904, 919
NlaIII	CATG	9 cut(s)	188, 254, 284, 643, 926, 990, 1075, 1251, 1458
NlaIV	GGNNCC	2 cut(s)	535, 721
NmuCI	GTSAC	6 cut(s)	475, 605, 730, 754, 1174, 1349
NspI	RCATGY	2 cut(s)	254, 1251
PceI	AGGCCT	1 cut(s)	1380
PciI	ACATGT	1 cut(s)	1247
PciSI	GCTCTTC	1 cut(s)	149
PctI	GAATGC	2 cut(s)	372, 1315
PfeI	GAWTC	2 cut(s)	226, 689
PinAI	ACCGGT	2 cut(s)	794, 1512
PleI	GAGTC	1 cut(s)	1102
PpsI	GAGTC	1 cut(s)	1102
PscI	ACATGT	1 cut(s)	1247
Psp124BI	GAGCTC	1 cut(s)	144
Psp6I	CCWGG	1 cut(s)	1345
PspGI	CCWGG	1 cut(s)	1345
PspN4I	GGNNCC	2 cut(s)	535, 721
PspPI	GGNCC	2 cut(s)	361, 490
PstI	CTGCAG	2 cut(s)	271, 629
PstNI	CAGNNNCTG	1 cut(s)	692
PvuII	CAGCTG	2 cut(s)	122, 1238
RsaI	GTAC	2 cut(s)	86, 721
RsaNI	GTAC	2 cut(s)	85, 720
SacI	GAGCTC	1 cut(s)	144
SapI	GCTCTTC	1 cut(s)	149
SaqAI	TTAA	2 cut(s)	486, 1389
Sau3AI	GATC	3 cut(s)	372, 904, 919
Sau96I	GGNCC	2 cut(s)	361, 490
ScaI	AGTACT	1 cut(s)	86
SchI	GAGTC	1 cut(s)	1103
ScrFI	CCNGG	1 cut(s)	1347
SduI	GDGCHC	1 cut(s)	144
SfcI	CTRYAG	3 cut(s)	267, 625, 1496
SinI	GGWCC	2 cut(s)	361, 490
SmlI	CTYRAG	1 cut(s)	1388
SmoI	CTYRAG	1 cut(s)	1388
SpeI	ACTAGT	1 cut(s)	995
Sse9I	AATT	7 cut(s)	15, 214, 471, 863, 879, 982, 1265
SseBI	AGGCCT	1 cut(s)	1380
SsiI	CCGC	6 cut(s)	467, 809, 1166, 1181, 1215, 1313
SspMI	CTAG	3 cut(s)	53, 78, 996
SstI	GAGCTC	1 cut(s)	144
StuI	AGGCCT	1 cut(s)	1380
StyD4I	CCNGG	1 cut(s)	1345
StyI	CCWWGG	4 cut(s)	493, 735, 1071, 1454
TaaI	ACNGT	4 cut(s)	109, 890, 959, 1500
TaqI	TCGA	4 cut(s)	453, 540, 762, 1371
TasI	AATT	7 cut(s)	15, 214, 471, 863, 879, 982, 1265
TatI	WGTACW	1 cut(s)	84
TfiI	GAWTC	2 cut(s)	226, 689
Tru1I	TTAA	2 cut(s)	486, 1389
Tru9I	TTAA	2 cut(s)	486, 1389
TscAI	CASTG	6 cut(s)	535, 561, 634, 1179, 1330, 1505
TseFI	GTSAC	6 cut(s)	475, 605, 730, 754, 1174, 1349
Tsp45I	GTSAC	6 cut(s)	475, 605, 730, 754, 1174, 1349
TspDTI	ATGAA	5 cut(s)	28, 975, 1068, 1278, 1523
TspRI	CASTG	6 cut(s)	535, 561, 634, 1179, 1330, 1505
Vha464I	CTTAAG	1 cut(s)	1388
VpaK11BI	GGWCC	2 cut(s)	361, 490
XapI	RAATTY	4 cut(s)	214, 879, 982, 1265
XceI	RCATGY	2 cut(s)	254, 1251
XcmI	CCANNNNNNNNNTGG	1 cut(s)	500
XspI	CTAG	3 cut(s)	53, 78, 996
ZrmI	AGTACT	1 cut(s)	86

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.