Prupe.4G065300_v2.0.a1

Rho termination factor, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013074
Physical Location & Seq
Reverse (-)
3134374 .. 3136013
1640 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G065300.1

Sequence Viewer

Length: 750 bp
ATGGGTGCAATTGTTCATGAATATCATTCTGTGCTTCGCTTTCCCACTTTCTTCTCTTTCAGCAAGCAGCCCAAGCTGGGAAAATCCATTTTCTCTTTGAAAGATGTGCCTTCACCCTTTGAGCGAAAGGGCCTTCACTTAACTGTGTCCTGCATTGGATCTGAAGGAAATGGAGGAGACCAACCTCCTCGGAGGCGTTCAGCTTCAGGAAGAACGAATAAGAACGATGGAACCAAGAAACGTGGACCAGCTGGTAAAAAATCTAAGGCATCTGACCAGGAAGAGATCATTTCTCTATTCAGACGGATACAGACTTCGATCTCGAACAAAGAGTCTGTAAATGCTAAGAAGATAAATTCCAATGTCTCTGAAGATAGCCCCTCTTCTGAGTCAATTCTTCAGGCTCTTTATGGATCGAGGAAGCAAAAAGGCAAAGCTTTAGACAAGGCAGGGCAAGAGGTCTGGACAAGGAGGAAAGATACGCAAGAGCAGCAAATACAGGAGGATCCCTCAGTTGCAGAGTTCAAGTTAACGCGCCCACCGTCTAAATTTGTGAAAAGATCTCCGATTCCATCTCAATCAATCCCAAGAGGACAAGTTCTTGAGCTGAATAACGGAGCATCATCTTCTGCGGCTGGAAGAATAGAAGAAATGAAACTCCCTGAGTTGAAAGAACTGGCAAAGACCAGAGGAATTAAAGGTTACTCGAAACTTAAAAAGAGCGAGCTCGTTCAACTGCTGAAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

250

Amino Acids

27.52

Weight (kDa)

10.12

Isoelectric Point (pI)

60.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18740 AT4G18740 AT4G18740 AT4G18740
fragaria_vesca FvH4_3g07440
malus_domestica MD05G1297000.v1.1
prunus_persica Prupe.4G065300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011411
rosa_laevigata RLG00000031831
rosa_multiflora Rmu_co8186696.1_g000001 Rmu_sc0009975.1_g000015
rosa_roxburghii Rroxscaffold_1G00065040
rosa_rugosa Rorug04G0456900
rosa_samantha Rh5AG088700 Rh5BG083700 Rh5CG097000 Rh5DG083700
rosa_wichuraiana Rw5G007700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 535
AciI CCGC 1 cut(s) 632
AclWI GGATC 4 cut(s) 166, 421, 500, 513
AcsI RAATTY 2 cut(s) 355, 548
AcuI CTGAAG 4 cut(s) 183, 189, 383, 390
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 4 cut(s) 100, 526, 670, 734
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 6 cut(s) 76, 203, 251, 437, 607, 727
AluI AGCT 6 cut(s) 76, 203, 251, 437, 607, 727
Alw21I GWGCWC 1 cut(s) 729
Alw26I GTCTC 2 cut(s) 171, 370
AlwI GGATC 4 cut(s) 166, 421, 500, 513
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 67, 490
ApoI RAATTY 2 cut(s) 355, 548
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 2 cut(s) 130, 245
AsuHPI GGTGA 1 cut(s) 105
AvaII GGWCC 1 cut(s) 245
BamHI GGATCC 1 cut(s) 505
BanII GRGCYC 1 cut(s) 729
Bbv12I GWGCWC 1 cut(s) 729
BbvI GCAGC 2 cut(s) 79, 502
BccI CCATC 2 cut(s) 221, 580
BciT130I CCWGG 1 cut(s) 278
BciVI GTATCC 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 171, 370
BfuI GTATCC 1 cut(s) 300
BglII AGATCT 1 cut(s) 560
BisI GCNGC 3 cut(s) 68, 491, 633
BlsI GCNGC 3 cut(s) 69, 492, 634
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 130, 245
BmiI GGNNCC 2 cut(s) 232, 507
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 2 cut(s) 278, 629
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpuEI CTTGAG 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 681
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 3 cut(s) 378, 525, 654
BseNI ACTGG 1 cut(s) 681
BseRI GAGGAG 2 cut(s) 177, 189
BseXI GCAGC 2 cut(s) 79, 502
BseYI CCCAGC 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 535
BshFI GGCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 729
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 2 cut(s) 171, 370
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 729
Bsp143I GATC 6 cut(s) 158, 285, 318, 413, 505, 560
BspACI CCGC 1 cut(s) 632
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 3 cut(s) 379, 524, 655
BspFNI CGCG 1 cut(s) 535
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 2 cut(s) 232, 507
BspPI GGATC 4 cut(s) 166, 421, 500, 513
BspTNI GGTCTC 1 cut(s) 171
BsrI ACTGG 1 cut(s) 681
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 6 cut(s) 158, 285, 318, 413, 505, 560
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 2 cut(s) 145, 543
Bst6I CTCTTC 2 cut(s) 276, 388
BstC8I GCNNGC 2 cut(s) 65, 725
BstDEI CTNAG 5 cut(s) 264, 345, 387, 511, 663
BstFNI CGCG 1 cut(s) 535
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 6 cut(s) 161, 288, 321, 416, 508, 563
BstMAI GTCTC 2 cut(s) 171, 370
BstMBI GATC 6 cut(s) 158, 285, 318, 413, 505, 560
BstMWI GCNNNNNNNGC 2 cut(s) 73, 490
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstUI CGCG 1 cut(s) 535
BstV1I GCAGC 2 cut(s) 79, 502
BstX2I RGATCY 3 cut(s) 158, 505, 560
BstYI RGATCY 3 cut(s) 158, 505, 560
BsuI GTATCC 1 cut(s) 300
BsuRI GGCC 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 65, 725
CciI TCATGA 1 cut(s) 16
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 2 cut(s) 130, 245
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 1 cut(s) 17
DdeI CTNAG 5 cut(s) 264, 345, 387, 511, 663
DpnI GATC 6 cut(s) 160, 287, 320, 415, 507, 562
DpnII GATC 6 cut(s) 158, 285, 318, 413, 505, 560
Eam1104I CTCTTC 2 cut(s) 276, 388
EarI CTCTTC 2 cut(s) 276, 388
Ecl136II GAGCTC 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 727
Eco57I CTGAAG 4 cut(s) 183, 189, 383, 390
EcoICRI GAGCTC 1 cut(s) 727
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 729
FaeI CATG 1 cut(s) 20
FaiI YATR 2 cut(s) 18, 411
FatI CATG 1 cut(s) 16
Fnu4HI GCNGC 3 cut(s) 68, 491, 633
FriOI GRGCYC 1 cut(s) 729
Fsp4HI GCNGC 3 cut(s) 68, 491, 633
GlaI GCGC 1 cut(s) 536
GluI GCNGC 3 cut(s) 68, 491, 633
GsaI CCCAGC 1 cut(s) 80
HaeIII GGCC 1 cut(s) 132
HhaI GCGC 1 cut(s) 537
Hin1II CATG 1 cut(s) 20
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HincII GTYRAC 1 cut(s) 531
HindII GTYRAC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 3 cut(s) 332, 389, 568
HpaI GTTAAC 1 cut(s) 531
HphI GGTGA 1 cut(s) 105
Hpy166II GTNNAC 2 cut(s) 245, 531
Hpy188I TCNGA 7 cut(s) 163, 192, 274, 302, 370, 388, 567
Hpy188III TCNNGA 5 cut(s) 17, 207, 322, 463, 602
Hpy8I GTNNAC 2 cut(s) 245, 531
HpyAV CCTTC 3 cut(s) 120, 143, 158
HpyCH4III ACNGT 2 cut(s) 145, 543
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 3 cut(s) 8, 153, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 490
HpyF3I CTNAG 5 cut(s) 264, 345, 387, 511, 663
HpySE526I ACGT 1 cut(s) 241
Hsp92II CATG 1 cut(s) 20
HspAI GCGC 1 cut(s) 535
KspAI GTTAAC 1 cut(s) 531
Kzo9I GATC 6 cut(s) 158, 285, 318, 413, 505, 560
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 2 cut(s) 79, 502
LweI GCATC 2 cut(s) 278, 629
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 701
MalI GATC 6 cut(s) 160, 287, 320, 415, 507, 562
MboI GATC 6 cut(s) 158, 285, 318, 413, 505, 560
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 3 cut(s) 158, 505, 560
MhlI GDGCHC 1 cut(s) 729
MluCI AATT 5 cut(s) 9, 355, 393, 548, 693
MlyI GAGTC 2 cut(s) 341, 398
MseI TTAA 4 cut(s) 140, 530, 696, 714
MspA1I CMGCKG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 535
MwoI GCNNNNNNNGC 2 cut(s) 73, 490
NdeII GATC 6 cut(s) 158, 285, 318, 413, 505, 560
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 2 cut(s) 232, 507
PagI TCATGA 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 539
PfeI GAWTC 1 cut(s) 568
PkrI GCNGC 3 cut(s) 69, 492, 634
PleI GAGTC 2 cut(s) 340, 397
PpsI GAGTC 2 cut(s) 340, 397
Psp124BI GAGCTC 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 2 cut(s) 232, 507
PspPI GGNCC 2 cut(s) 130, 245
PsuI RGATCY 3 cut(s) 158, 505, 560
PvuII CAGCTG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 729
SaqAI TTAA 4 cut(s) 140, 530, 696, 714
SatI GCNGC 3 cut(s) 68, 491, 633
Sau3AI GATC 6 cut(s) 158, 285, 318, 413, 505, 560
Sau96I GGNCC 2 cut(s) 130, 245
SchI GAGTC 2 cut(s) 341, 398
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 1 cut(s) 729
SfaNI GCATC 2 cut(s) 278, 629
SinI GGWCC 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 5 cut(s) 9, 355, 393, 548, 693
SsiI CCGC 1 cut(s) 632
SstI GAGCTC 1 cut(s) 729
StyD4I CCNGG 1 cut(s) 276
TaaI ACNGT 2 cut(s) 145, 543
TaiI ACGT 1 cut(s) 244
TaqI TCGA 4 cut(s) 317, 323, 416, 707
TasI AATT 5 cut(s) 9, 355, 393, 548, 693
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 4 cut(s) 140, 530, 696, 714
Tru9I TTAA 4 cut(s) 140, 530, 696, 714
TseI GCWGC 2 cut(s) 67, 490
TspDTI ATGAA 3 cut(s) 5, 33, 668
TspGWI ACGGA 2 cut(s) 319, 630
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 2 cut(s) 355, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.