RchiOBHm_Chr5g0011411

Rho termination factor, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013074
Physical Location & Seq
Reverse (-)
7557283 .. 7558859
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29210

Sequence Viewer

Length: 780 bp
ATGGGTGGAATTGTGTATCAGTATCACTCTGTGCTTCGGTACCCGGCATTCTTATCTTTCAGTAAGCAGCCCAAGCTGGGAAAACCTATTTTCTCACTCAAAGACATTGCAGATGTGTCTTCACCGTTTGAGAGGAAGGGGCTGAAGTTAACCGTGTCCTGCGTTGGATCTGATGAAGGAAGCGGAAGAGGTCAATCTTCTAGGAGAAGTTCAGGTTCAGGAAGAACACGGAAAAACGATGAAACTAAGAAAGCTCGAGGTGGGAGAAAGTCCAAGTCATCTAATCAGGAAGAAATCATTTCTCTGTTCAGGCGGATACAGTCATCGATATCGAAAGAAGTAGACTCTGTAGATACTGAGAAGATAAACTCCAATGCATCGGAAGAGAAGCCGCCCTCTGCTGAGTCAATTCTTAGGGTTCTTCAAGGTGGATCAATAAAGCAAAAAGAAGAAGCTAACCAGGAAAGAGAAAAGGTTGATATGAAAGAGGAGCAAATACAGGCTAATCCATCAATTACAGACTTCAAGTTAATGAGGCCACCATCCAAATTCGTGAAGAGGTCTCCAATTCCGTCTTCATCAAACAATTCAAGCCCACCAAGAAAGCTTCTTGAGATGAATAATGGAGCATCACTTGCCACAGCTGGAACCAAGGAGTTAGAGTTGGAAACAATAGAAGAAATGAAACTCCCTGAGCTGAAAGAACTGGCAAAGTCTAGAGGGATCAGAGGTTATTCAAAACTCAAAAAGAGGGAGCTTGTGGAACTGTTGAAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

259

Amino Acids

28.76

Weight (kDa)

9.79

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 226 - 258 1.8e-08 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18740 AT4G18740 AT4G18740 AT4G18740
fragaria_vesca FvH4_3g07440
malus_domestica MD05G1297000.v1.1
prunus_persica Prupe.4G065300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011411
rosa_laevigata RLG00000031831
rosa_multiflora Rmu_co8186696.1_g000001 Rmu_sc0009975.1_g000015
rosa_roxburghii Rroxscaffold_1G00065040
rosa_rugosa Rorug04G0456900
rosa_samantha Rh5AG088700 Rh5BG083700 Rh5CG097000 Rh5DG083700
rosa_wichuraiana Rw5G007700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 342
AciI CCGC 3 cut(s) 183, 313, 392
AclWI GGATC 3 cut(s) 175, 439, 731
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 164
AdeI CACNNNGTG 1 cut(s) 31
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 5 cut(s) 425, 526, 591, 738, 772
AjnI CCWGG 1 cut(s) 459
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AluBI AGCT 7 cut(s) 76, 254, 455, 607, 644, 697, 757
AluI AGCT 7 cut(s) 76, 254, 455, 607, 644, 697, 757
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 3 cut(s) 175, 439, 731
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 548
Asp718I GGTACC 1 cut(s) 39
AsuC2I CCSGG 1 cut(s) 44
AsuHPI GGTGA 1 cut(s) 114
AvaI CYCGRG 1 cut(s) 255
BanI GGYRCC 1 cut(s) 39
BbsI GAAGAC 2 cut(s) 111, 567
BbvI GCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 517, 550
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 309
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 2 cut(s) 201, 717
BfmI CTRYAG 1 cut(s) 348
BfuI GTATCC 1 cut(s) 309
BisI GCNGC 2 cut(s) 68, 392
BlsI GCNGC 2 cut(s) 69, 393
Bme1390I CCNGG 2 cut(s) 44, 461
BmeT110I CYCGRG 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 41, 649
BmrFI CCNGG 2 cut(s) 44, 461
BmsI GCATC 2 cut(s) 386, 638
BpiI GAAGAC 2 cut(s) 111, 567
Bpu10I CCTNAGC 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 44
Bsa29I ATCGAT 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 711
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 461
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 651
BseGI GGATG 1 cut(s) 542
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 348, 393, 684
BseNI ACTGG 1 cut(s) 711
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 76
BshFI GGCC 1 cut(s) 538
BshNI GGYRCC 1 cut(s) 39
BshVI ATCGAT 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 567
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 1 cut(s) 538
Bso31I GGTCTC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 255
Bsp143I GATC 3 cut(s) 167, 431, 723
BspACI CCGC 3 cut(s) 183, 313, 392
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 3 cut(s) 349, 394, 685
BspDI ATCGAT 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 41, 649
BspPI GGATC 3 cut(s) 175, 439, 731
BspT107I GGYRCC 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 105
BsrI ACTGG 1 cut(s) 711
BssECI CCNNGG 1 cut(s) 651
BssMI GATC 3 cut(s) 167, 431, 723
BssT1I CCWWGG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 126, 154, 321, 768
Bst6I CTCTTC 3 cut(s) 181, 378, 551
BstAPI GCANNNNNTGC 1 cut(s) 635
BstDEI CTNAG 5 cut(s) 246, 357, 402, 413, 693
BstF5I GGATG 1 cut(s) 542
BstKTI GATC 3 cut(s) 170, 434, 726
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 3 cut(s) 167, 431, 723
BstMWI GCNNNNNNNGC 2 cut(s) 73, 635
BstNI CCWGG 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 42, 459
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 2 cut(s) 111, 567
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
Bsu15I ATCGAT 1 cut(s) 326
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 1 cut(s) 538
BsuTUI ATCGAT 1 cut(s) 326
BtsCI GGATG 1 cut(s) 542
ClaI ATCGAT 1 cut(s) 326
Csp6I GTAC 1 cut(s) 40
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 5 cut(s) 246, 357, 402, 413, 693
DpnI GATC 3 cut(s) 169, 433, 725
DpnII GATC 3 cut(s) 167, 431, 723
DraIII CACNNNGTG 1 cut(s) 31
Eam1104I CTCTTC 3 cut(s) 181, 378, 551
EarI CTCTTC 3 cut(s) 181, 378, 551
EciI GGCGGA 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 651
Eco31I GGTCTC 1 cut(s) 567
Eco32I GATATC 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 459
EcoRV GATATC 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 651
EcoT22I ATGCAT 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 651
FaiI YATR 1 cut(s) 482
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 68, 392
FokI GGATG 1 cut(s) 529
Fsp4HI GCNGC 2 cut(s) 68, 392
FspBI CTAG 2 cut(s) 201, 717
GluI GCNGC 2 cut(s) 68, 392
GsaI CCCAGC 1 cut(s) 80
HaeIII GGCC 1 cut(s) 538
HapII CCGG 1 cut(s) 44
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 2 cut(s) 344, 404
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 114
Hpy166II GTNNAC 2 cut(s) 150, 343
Hpy188I TCNGA 3 cut(s) 172, 382, 728
Hpy188III TCNNGA 5 cut(s) 219, 287, 553, 611, 717
Hpy8I GTNNAC 2 cut(s) 150, 343
HpyAV CCTTC 2 cut(s) 130, 170
HpyCH4III ACNGT 4 cut(s) 126, 154, 321, 768
HpyCH4V TGCA 2 cut(s) 110, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 635
HpyF3I CTNAG 5 cut(s) 246, 357, 402, 413, 693
KpnI GGTACC 1 cut(s) 43
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 3 cut(s) 167, 431, 723
LmnI GCTCC 3 cut(s) 490, 626, 754
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 2 cut(s) 386, 638
MaeI CTAG 2 cut(s) 201, 717
MalI GATC 3 cut(s) 169, 433, 725
MboI GATC 3 cut(s) 167, 431, 723
MflI RGATCY 1 cut(s) 167
MluCI AATT 6 cut(s) 9, 408, 513, 548, 567, 586
MlyI GAGTC 2 cut(s) 338, 413
MmeI TCCRAC 2 cut(s) 145, 645
Mph1103I ATGCAT 1 cut(s) 379
MseI TTAA 2 cut(s) 149, 530
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 2 cut(s) 44, 461
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 73, 635
NciI CCSGG 1 cut(s) 44
NdeII GATC 3 cut(s) 167, 431, 723
NlaIV GGNNCC 2 cut(s) 41, 649
NsiI ATGCAT 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 255
PctI GAATGC 1 cut(s) 47
PkrI GCNGC 2 cut(s) 69, 393
PleI GAGTC 2 cut(s) 338, 412
PpsI GAGTC 2 cut(s) 338, 412
Psp6I CCWGG 1 cut(s) 459
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 2 cut(s) 41, 649
PspXI VCTCGAGB 1 cut(s) 255
PsuI RGATCY 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SaqAI TTAA 2 cut(s) 149, 530
SatI GCNGC 2 cut(s) 68, 392
Sau3AI GATC 3 cut(s) 167, 431, 723
SchI GAGTC 2 cut(s) 338, 413
ScrFI CCNGG 2 cut(s) 44, 461
SfaNI GCATC 2 cut(s) 386, 638
SfcI CTRYAG 1 cut(s) 348
Sfr274I CTCGAG 1 cut(s) 255
SlaI CTCGAG 1 cut(s) 255
SmlI CTYRAG 2 cut(s) 255, 611
SmoI CTYRAG 2 cut(s) 255, 611
Sse9I AATT 6 cut(s) 9, 408, 513, 548, 567, 586
SsiI CCGC 3 cut(s) 183, 313, 392
SspMI CTAG 2 cut(s) 201, 717
StyD4I CCNGG 2 cut(s) 42, 459
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 4 cut(s) 126, 154, 321, 768
TaqI TCGA 3 cut(s) 256, 326, 332
TasI AATT 6 cut(s) 9, 408, 513, 548, 567, 586
TauI GCSGC 1 cut(s) 394
Tru1I TTAA 2 cut(s) 149, 530
Tru9I TTAA 2 cut(s) 149, 530
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 6 cut(s) 189, 255, 497, 567, 632, 698
TspGWI ACGGA 2 cut(s) 244, 561
XapI RAATTY 1 cut(s) 548
XbaI TCTAGA 1 cut(s) 716
XhoI CTCGAG 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 2 cut(s) 201, 717
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.