RLG00000031831

Rho termination factor, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013074
Physical Location & Seq
Reverse (-)
8572962 .. 8574269
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031831

Sequence Viewer

Length: 780 bp
ATGGGTGGAATTGTGTATCAGTATCACTCTGTGCTTCGCTTTCCGGCATTCTTATCTTTCAGTAAGCAGCCCAAGCTGGGAAAACCTATTTTCTCAATCAAAGACATTGCAGATGTGTCTTCACCGTTTGAGAGGAAGGGGCTGAAGTTAACTGTGTCCTGCGTTGGATCTGATGAAGGAAGCGGAAGAGGTCAATCTTCTAGGAGAAGTTCAGGTTCAGGAAGAACGAGGAAAAATGATGAAACTAAGAAAGCTCGAGGTGGGAGAAAGTCCAAGTCATCTAATCAGGAAGAAATCATTTCTCTGTTCAGGCGGATACAGTCATCGATATCGAAAGAAGTAGACTCTGTAGATACTAAGAAGATAAACTCCAGGGCATCGGAAGAGAAGCCGCCCTCTGCTGAGTCAATTCTTAGGGTTCTTCAGGGTGGATCAACAAAGCAAAAAGAAGAAGCTAACCAGGAAAGAGAAAAGCTTGATATGAAAGAGGAGCAAATACAGGCTAATCCATCAATTACAGACTTCAAGTTAACGAGGCCACCATCCAAATTCGTGAAGAGGTCTCCAATTCCGTCTTCATCAAACAGTTCAAGCCCACCAGGAAAGCTTCTTGGGATGAATAATGGAGCATCACTTGCCACTACTGGAACCAAGGAGTTAGAGTTGGAAAGAGTTGAAGAAATGAAACTCCCTGAGCTGAAAGAACTGGCAAAGTCTAGAGGGATCAGAGGTTATTCAAAACTCAAAAAGAGGGAGCTTGTGGAACTGTTGAAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

260

Amino Acids

28.63

Weight (kDa)

9.99

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 226 - 258 2e-08 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18740 AT4G18740 AT4G18740 AT4G18740
fragaria_vesca FvH4_3g07440
malus_domestica MD05G1297000.v1.1
prunus_persica Prupe.4G065300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011411
rosa_laevigata RLG00000031831
rosa_multiflora Rmu_co8186696.1_g000001 Rmu_sc0009975.1_g000015
rosa_roxburghii Rroxscaffold_1G00065040
rosa_rugosa Rorug04G0456900
rosa_samantha Rh5AG088700 Rh5BG083700 Rh5CG097000 Rh5DG083700
rosa_wichuraiana Rw5G007700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 342
AciI CCGC 3 cut(s) 183, 313, 392
AclWI GGATC 3 cut(s) 175, 439, 731
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 2 cut(s) 164, 407
AdeI CACNNNGTG 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 5 cut(s) 526, 591, 677, 738, 772
AjnI CCWGG 3 cut(s) 371, 459, 598
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AluBI AGCT 7 cut(s) 76, 254, 455, 475, 607, 697, 757
AluI AGCT 7 cut(s) 76, 254, 455, 475, 607, 697, 757
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 3 cut(s) 175, 439, 731
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 548
AsuHPI GGTGA 1 cut(s) 114
AvaI CYCGRG 1 cut(s) 255
BbsI GAAGAC 2 cut(s) 111, 567
BbvI GCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 517, 550
BciT130I CCWGG 3 cut(s) 373, 461, 600
BciVI GTATCC 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 2 cut(s) 201, 717
BfmI CTRYAG 1 cut(s) 348
BfuI GTATCC 1 cut(s) 309
BisI GCNGC 2 cut(s) 68, 392
BlsI GCNGC 2 cut(s) 69, 393
Bme1390I CCNGG 3 cut(s) 373, 461, 600
BmeT110I CYCGRG 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 649
BmrFI CCNGG 3 cut(s) 373, 461, 600
BmsI GCATC 2 cut(s) 386, 638
BpiI GAAGAC 2 cut(s) 111, 567
BpmI CTGGAG 1 cut(s) 355
Bpu10I CCTNAGC 1 cut(s) 693
Bsa29I ATCGAT 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 372, 651
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 649, 711
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 3 cut(s) 373, 461, 600
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 372, 651
BseGI GGATG 2 cut(s) 542, 621
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 393, 684
BseNI ACTGG 2 cut(s) 649, 711
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 76
BshFI GGCC 1 cut(s) 538
BshVI ATCGAT 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 567
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 1 cut(s) 538
Bso31I GGTCTC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 255
Bsp143I GATC 3 cut(s) 167, 431, 723
BspACI CCGC 3 cut(s) 183, 313, 392
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 2 cut(s) 394, 685
BspDI ATCGAT 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 649
BspPI GGATC 3 cut(s) 175, 439, 731
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 105
BsrI ACTGG 2 cut(s) 649, 711
BssECI CCNNGG 2 cut(s) 372, 651
BssMI GATC 3 cut(s) 167, 431, 723
BssT1I CCWWGG 1 cut(s) 651
Bst2UI CCWGG 3 cut(s) 373, 461, 600
Bst4CI ACNGT 5 cut(s) 126, 154, 321, 587, 768
Bst6I CTCTTC 3 cut(s) 181, 378, 551
BstAPI GCANNNNNTGC 1 cut(s) 635
BstDEI CTNAG 5 cut(s) 246, 357, 402, 413, 693
BstF5I GGATG 2 cut(s) 542, 621
BstKTI GATC 3 cut(s) 170, 434, 726
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 3 cut(s) 167, 431, 723
BstMWI GCNNNNNNNGC 2 cut(s) 73, 635
BstNI CCWGG 3 cut(s) 373, 461, 600
BstSCI CCNGG 3 cut(s) 371, 459, 598
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 2 cut(s) 111, 567
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
Bsu15I ATCGAT 1 cut(s) 326
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 1 cut(s) 538
BsuTUI ATCGAT 1 cut(s) 326
BtsCI GGATG 2 cut(s) 542, 621
ClaI ATCGAT 1 cut(s) 326
DdeI CTNAG 5 cut(s) 246, 357, 402, 413, 693
DpnI GATC 3 cut(s) 169, 433, 725
DpnII GATC 3 cut(s) 167, 431, 723
DraIII CACNNNGTG 1 cut(s) 31
Eam1104I CTCTTC 3 cut(s) 181, 378, 551
EarI CTCTTC 3 cut(s) 181, 378, 551
EciI GGCGGA 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 651
Eco31I GGTCTC 1 cut(s) 567
Eco32I GATATC 1 cut(s) 330
Eco57I CTGAAG 2 cut(s) 164, 407
Eco88I CYCGRG 1 cut(s) 255
EcoRII CCWGG 3 cut(s) 371, 459, 598
EcoRV GATATC 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 651
FaiI YATR 1 cut(s) 482
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 68, 392
FokI GGATG 2 cut(s) 529, 628
Fsp4HI GCNGC 2 cut(s) 68, 392
FspBI CTAG 2 cut(s) 201, 717
GluI GCNGC 2 cut(s) 68, 392
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 355
HaeIII GGCC 1 cut(s) 538
HapII CCGG 1 cut(s) 44
HincII GTYRAC 2 cut(s) 150, 531
HindII GTYRAC 2 cut(s) 150, 531
HindIII AAGCTT 2 cut(s) 473, 605
HinfI GANTC 2 cut(s) 344, 404
HpaI GTTAAC 2 cut(s) 150, 531
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 114
Hpy166II GTNNAC 3 cut(s) 150, 343, 531
Hpy188I TCNGA 3 cut(s) 172, 382, 728
Hpy188III TCNNGA 4 cut(s) 219, 287, 553, 717
Hpy8I GTNNAC 3 cut(s) 150, 343, 531
HpyAV CCTTC 2 cut(s) 130, 170
HpyCH4III ACNGT 5 cut(s) 126, 154, 321, 587, 768
HpyCH4V TGCA 1 cut(s) 110
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 635
HpyF3I CTNAG 5 cut(s) 246, 357, 402, 413, 693
KspAI GTTAAC 2 cut(s) 150, 531
Kzo9I GATC 3 cut(s) 167, 431, 723
LmnI GCTCC 3 cut(s) 490, 626, 754
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 2 cut(s) 386, 638
MaeI CTAG 2 cut(s) 201, 717
MalI GATC 3 cut(s) 169, 433, 725
MboI GATC 3 cut(s) 167, 431, 723
MflI RGATCY 1 cut(s) 167
MluCI AATT 5 cut(s) 9, 408, 513, 548, 567
MlyI GAGTC 2 cut(s) 338, 413
MmeI TCCRAC 2 cut(s) 145, 645
MseI TTAA 2 cut(s) 149, 530
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 3 cut(s) 373, 461, 600
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 3 cut(s) 373, 461, 600
MwoI GCNNNNNNNGC 2 cut(s) 73, 635
NdeII GATC 3 cut(s) 167, 431, 723
NlaIV GGNNCC 1 cut(s) 649
PaeR7I CTCGAG 1 cut(s) 255
PctI GAATGC 1 cut(s) 47
PkrI GCNGC 2 cut(s) 69, 393
PleI GAGTC 2 cut(s) 338, 412
PpsI GAGTC 2 cut(s) 338, 412
Psp6I CCWGG 3 cut(s) 371, 459, 598
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 3 cut(s) 371, 459, 598
PspN4I GGNNCC 1 cut(s) 649
PspXI VCTCGAGB 1 cut(s) 255
PsuI RGATCY 1 cut(s) 167
SaqAI TTAA 2 cut(s) 149, 530
SatI GCNGC 2 cut(s) 68, 392
Sau3AI GATC 3 cut(s) 167, 431, 723
SchI GAGTC 2 cut(s) 338, 413
ScrFI CCNGG 3 cut(s) 373, 461, 600
SfaNI GCATC 2 cut(s) 386, 638
SfcI CTRYAG 1 cut(s) 348
Sfr274I CTCGAG 1 cut(s) 255
SlaI CTCGAG 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 5 cut(s) 9, 408, 513, 548, 567
SsiI CCGC 3 cut(s) 183, 313, 392
SspMI CTAG 2 cut(s) 201, 717
StyD4I CCNGG 3 cut(s) 371, 459, 598
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 5 cut(s) 126, 154, 321, 587, 768
TaqI TCGA 3 cut(s) 256, 326, 332
TasI AATT 5 cut(s) 9, 408, 513, 548, 567
TauI GCSGC 1 cut(s) 394
Tru1I TTAA 2 cut(s) 149, 530
Tru9I TTAA 2 cut(s) 149, 530
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 6 cut(s) 189, 255, 497, 567, 632, 698
TspGWI ACGGA 1 cut(s) 561
XapI RAATTY 1 cut(s) 548
XbaI TCTAGA 1 cut(s) 716
XhoI CTCGAG 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 2 cut(s) 201, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.