Rw5G007700

Rho termination factor, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013074
Physical Location & Seq
Reverse (-)
8168159 .. 8169460
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007700.1

Sequence Viewer

Length: 786 bp
ATGGGTGGAATTGTGTATCAGTATCACTCTGTGCTTCGCTTTCCGGCATTCTTATCTTTCAGTAAGCAGCCCAAGCTGGGTAAAGGAAAACCTATTTTCTCACTCAAAGACATTGCAGATGTGTCTTCACCGTTTGAGAGGAAGGGGCTGAAGTTAACAGTGTCCTGCGTTGGATCTGATGAAGGAAGCGGAAGAGGTCAATCTTCTAGGAGAAGTTCAGGTTCAGGAAGAACAAGGAAAAATGATGAACCTAAGAAAGCTCGAGGTGGGAGAAAGTCCAAGTCATCTAACCAGGAAGAAATCATTTCTCTGTTCAGGCGGATACAGTCATCGATATCGAAAGAAGTAGACTCTGTAGATACTAAGAAGATAAACTCCAATGCATCAGAAGAGAAGCCGCCCTCGGCTGAGTCAATTCTTAGGGTTCTTCAAGGTGGATCAACAAAGCAAAGAGAAGAAGCTAACCAGGAAGGAGAAAAGGTTGTTATGAAAGAGCAGCAAATACAGGCTAATCTATCAGTTACAGACTTCAAGTTAATGCGGCCACCGTCCAAATTCGTGAAGAGGTCTCCAATTCCGTCTTCATCAAACAATTCAAGCCCACCACGAGAGCTTCTTGAGATGAATAATGGAGCAGCACTTGCCACTGCTGGAACCAAAGAGTTAGAGTTGGAAAGAGTAGAAGAAATGAAACTCCCTGAGCTGAAAGAACTAGCAAAGTCTAGGGGGATGGTAGGTTACTCAAAACTCAAAAAGAGGGAGCTTGTAGAACTGTTGAAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

261

Amino Acids

28.81

Weight (kDa)

9.92

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 228 - 260 1.6e-06 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18740 AT4G18740 AT4G18740 AT4G18740
fragaria_vesca FvH4_3g07440
malus_domestica MD05G1297000.v1.1
prunus_persica Prupe.4G065300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011411
rosa_laevigata RLG00000031831
rosa_multiflora Rmu_co8186696.1_g000001 Rmu_sc0009975.1_g000015
rosa_roxburghii Rroxscaffold_1G00065040
rosa_rugosa Rorug04G0456900
rosa_samantha Rh5AG088700 Rh5BG083700 Rh5CG097000 Rh5DG083700
rosa_wichuraiana Rw5G007700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 348
AciI CCGC 4 cut(s) 189, 319, 398, 541
AclWI GGATC 2 cut(s) 181, 445
AcoI YGGCCR 1 cut(s) 542
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 170
AdeI CACNNNGTG 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 4 cut(s) 431, 532, 597, 778
AjnI CCWGG 2 cut(s) 291, 465
AjuI GAANNNNNNNTTGG 2 cut(s) 565, 597
AluBI AGCT 6 cut(s) 76, 260, 461, 613, 703, 763
AluI AGCT 6 cut(s) 76, 260, 461, 613, 703, 763
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 2 cut(s) 181, 445
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 3 cut(s) 67, 496, 635
ApoI RAATTY 1 cut(s) 554
AsuHPI GGTGA 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 261
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 2 cut(s) 117, 573
BbvI GCAGC 3 cut(s) 79, 508, 647
BccI CCATC 1 cut(s) 724
BciT130I CCWGG 2 cut(s) 293, 467
BciVI GTATCC 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 3 cut(s) 207, 713, 723
BfmI CTRYAG 1 cut(s) 354
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 5 cut(s) 68, 398, 497, 542, 636
BlsI GCNGC 5 cut(s) 69, 399, 498, 543, 637
Bme1390I CCNGG 2 cut(s) 293, 467
BmeT110I CYCGRG 1 cut(s) 261
BmiI GGNNCC 1 cut(s) 655
BmrFI CCNGG 2 cut(s) 293, 467
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 2 cut(s) 117, 573
Bpu10I CCTNAGC 1 cut(s) 699
BpuEI CTTGAG 1 cut(s) 638
Bsa29I ATCGAT 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 2 cut(s) 293, 467
BseCI ATCGAT 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 735
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 399, 690
BseXI GCAGC 3 cut(s) 79, 508, 647
BseYI CCCAGC 1 cut(s) 76
BshFI GGCC 1 cut(s) 544
BshVI ATCGAT 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 1 cut(s) 544
Bso31I GGTCTC 1 cut(s) 573
BsoBI CYCGRG 1 cut(s) 261
Bsp143I GATC 2 cut(s) 173, 437
BspACI CCGC 4 cut(s) 189, 319, 398, 541
BspANI GGCC 1 cut(s) 544
BspCNI CTCAG 2 cut(s) 400, 691
BspDI ATCGAT 1 cut(s) 332
BspLI GGNNCC 1 cut(s) 655
BspPI GGATC 2 cut(s) 181, 445
BspTNI GGTCTC 1 cut(s) 573
BsrDI GCAATG 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 2 cut(s) 173, 437
BssSI CACGAG 1 cut(s) 606
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 2 cut(s) 293, 467
Bst4CI ACNGT 5 cut(s) 132, 160, 327, 549, 774
Bst6I CTCTTC 3 cut(s) 187, 384, 557
BstAPI GCANNNNNTGC 1 cut(s) 641
BstDEI CTNAG 5 cut(s) 252, 363, 408, 419, 699
BstF5I GGATG 1 cut(s) 735
BstKTI GATC 2 cut(s) 176, 440
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 2 cut(s) 173, 437
BstMWI GCNNNNNNNGC 2 cut(s) 73, 641
BstNI CCWGG 2 cut(s) 293, 467
BstSCI CCNGG 2 cut(s) 291, 465
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 3 cut(s) 79, 508, 647
BstV2I GAAGAC 2 cut(s) 117, 573
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
Bsu15I ATCGAT 1 cut(s) 332
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 1 cut(s) 544
BsuTUI ATCGAT 1 cut(s) 332
BtsCI GGATG 1 cut(s) 735
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 2 cut(s) 165, 645
ClaI ATCGAT 1 cut(s) 332
DdeI CTNAG 5 cut(s) 252, 363, 408, 419, 699
DpnI GATC 2 cut(s) 175, 439
DpnII GATC 2 cut(s) 173, 437
DraIII CACNNNGTG 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 542
Eam1104I CTCTTC 3 cut(s) 187, 384, 557
EarI CTCTTC 3 cut(s) 187, 384, 557
EciI GGCGGA 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 573
Eco32I GATATC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 291, 465
EcoRV GATATC 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 385
FaiI YATR 1 cut(s) 488
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 5 cut(s) 68, 398, 497, 542, 636
FokI GGATG 1 cut(s) 742
Fsp4HI GCNGC 5 cut(s) 68, 398, 497, 542, 636
FspBI CTAG 3 cut(s) 207, 713, 723
GluI GCNGC 5 cut(s) 68, 398, 497, 542, 636
GsaI CCCAGC 1 cut(s) 80
HaeIII GGCC 1 cut(s) 544
HapII CCGG 1 cut(s) 44
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 2 cut(s) 350, 410
HpaI GTTAAC 1 cut(s) 156
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 120
Hpy166II GTNNAC 2 cut(s) 156, 349
Hpy188I TCNGA 2 cut(s) 178, 388
Hpy188III TCNNGA 3 cut(s) 225, 559, 617
Hpy8I GTNNAC 2 cut(s) 156, 349
HpyAV CCTTC 3 cut(s) 136, 176, 464
HpyCH4III ACNGT 5 cut(s) 132, 160, 327, 549, 774
HpyCH4V TGCA 2 cut(s) 116, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 641
HpyF3I CTNAG 5 cut(s) 252, 363, 408, 419, 699
KspAI GTTAAC 1 cut(s) 156
Kzo9I GATC 2 cut(s) 173, 437
LmnI GCTCC 2 cut(s) 632, 760
Lsp1109I GCAGC 3 cut(s) 79, 508, 647
LweI GCATC 1 cut(s) 392
MaeI CTAG 3 cut(s) 207, 713, 723
MaeIII GTNAC 2 cut(s) 520, 737
MalI GATC 2 cut(s) 175, 439
MboI GATC 2 cut(s) 173, 437
MflI RGATCY 1 cut(s) 173
MluCI AATT 5 cut(s) 9, 414, 554, 573, 592
MlyI GAGTC 2 cut(s) 344, 419
MmeI TCCRAC 2 cut(s) 151, 651
MnlI CCTC 6 cut(s) 132, 188, 257, 412, 558, 750
Mph1103I ATGCAT 1 cut(s) 385
MseI TTAA 2 cut(s) 155, 536
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 2 cut(s) 293, 467
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 2 cut(s) 293, 467
MwoI GCNNNNNNNGC 2 cut(s) 73, 641
NdeII GATC 2 cut(s) 173, 437
NlaIV GGNNCC 1 cut(s) 655
NmeAIII GCCGAG 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 385
PaeR7I CTCGAG 1 cut(s) 261
PctI GAATGC 1 cut(s) 47
PkrI GCNGC 5 cut(s) 69, 399, 498, 543, 637
PleI GAGTC 2 cut(s) 344, 418
PpsI GAGTC 2 cut(s) 344, 418
Psp6I CCWGG 2 cut(s) 291, 465
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 2 cut(s) 291, 465
PspN4I GGNNCC 1 cut(s) 655
PspXI VCTCGAGB 1 cut(s) 261
PsuI RGATCY 1 cut(s) 173
SaqAI TTAA 2 cut(s) 155, 536
SatI GCNGC 5 cut(s) 68, 398, 497, 542, 636
Sau3AI GATC 2 cut(s) 173, 437
SchI GAGTC 2 cut(s) 344, 419
ScrFI CCNGG 2 cut(s) 293, 467
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 261
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 261, 617
SmoI CTYRAG 2 cut(s) 261, 617
Sse9I AATT 5 cut(s) 9, 414, 554, 573, 592
SsiI CCGC 4 cut(s) 189, 319, 398, 541
SspMI CTAG 3 cut(s) 207, 713, 723
StyD4I CCNGG 2 cut(s) 291, 465
TaaI ACNGT 5 cut(s) 132, 160, 327, 549, 774
TaqI TCGA 3 cut(s) 262, 332, 338
TasI AATT 5 cut(s) 9, 414, 554, 573, 592
TauI GCSGC 2 cut(s) 400, 544
Tru1I TTAA 2 cut(s) 155, 536
Tru9I TTAA 2 cut(s) 155, 536
TscAI CASTG 2 cut(s) 165, 652
TseI GCWGC 3 cut(s) 67, 496, 635
TspDTI ATGAA 6 cut(s) 195, 261, 503, 573, 638, 704
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 2 cut(s) 165, 652
XapI RAATTY 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 3 cut(s) 207, 713, 723
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.