Prupe.5G176300_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013665
Physical Location & Seq
Reverse (-)
14950008 .. 14953680
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G176300.3

Sequence Viewer

Length: 780 bp
ATGCCAGGCCCTGGTCCGCACCTCCTGTACGCCATGGGAACTGGCCTGGCTCTGACCACTCTCAGCAATGGCAGGTTCAGCCCCCACCACACCCTCTTCTACACCATCAACGCCTTCTTTGGCCCTGACATCGGATCCTTCTCTGAGTGGCTCGACTCCACCCTCGGCTTCGGCTTCGGCTCCAAGCTAGCCGACTTAATCCACCATCCCTTTTACTATGTTCTCTTTCCGGGTCTTCCTCTGTGTTTGCTGTACTCTTTGGTCTCTAGAGTTTTGCTTCAAAGACGGCTTCTTGATTCGTTTTCTCGGGTGCCCCTTACTAGGAAGCAGTGCTGGTTCTTGGTGTCAGCTGGTTCTTTTTCACACTTTTTCCTTGACCACTTGTTTGAGGAGAATGGGCATTCAAAAATGTATACTTGGATTTTGAGCACTGGTTGGTGGAAGGGCCGGGCACCGGTTAACCCAGATGCTGTTGTTGTAGTTGGCCTTTTATGCATTTTCTTAATCGGCGGCTTTATTTACATCAACAGGGTTAAGCCTCACAAGTCTATTAATAAGCAATCATATCAATCAGTGACACTGATTTCCATTGTAGCAATCCTGTATTGCTTATGGTGTGCAAGCCAAATACACTGGGTTAATCCTCGTCGGCCAGCAGTTGGTGAAGAGGCTGATCTTGGGGTTCTTGTATTTCTAGCCATTTACTTCTTTCTTCCTCACCTTTTGTGTATATTGTCCATGCACCCTATAGATCTTCAAGCAGAGCAACTTCCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.23

Weight (kDa)

8.53

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05110 FvH4_5g05110
malus_domestica MD06G1177600.v1.1
prunus_persica Prupe.5G176300_v2.0.a1 Prupe.5G176300_v2.0.a1 Prupe.5G176300_v2.0.a1
pyrus_communis pycom06g15760
rosa_chinensis RchiOBHm_Chr7g0193891
rosa_laevigata RLG00000004236
rosa_multiflora Rmu_sc0002389.1_g000014
rosa_roxburghii Rroxscaffold_3G00261730
rosa_rugosa Rorug07G0012500 Rorug07G0012500
rosa_samantha Rh7AG139400 Rh7BG139900 Rh7CG143600 Rh7DG140800
rosa_wichuraiana Rw7G011890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 63
AccB1I GGYRCC 2 cut(s) 310, 451
AccB7I CCANNNNNTGG 2 cut(s) 11, 659
AccI GTMKAC 1 cut(s) 413
AciI CCGC 2 cut(s) 17, 510
AclWI GGATC 2 cut(s) 129, 142
AcoI YGGCCR 1 cut(s) 650
AfaI GTAC 2 cut(s) 29, 254
AfiI CCNNNNNNNGG 5 cut(s) 11, 131, 321, 454, 659
AgeI ACCGGT 1 cut(s) 454
AgsI TTSAA 3 cut(s) 281, 405, 758
AjnI CCWGG 3 cut(s) 4, 10, 45
AluBI AGCT 2 cut(s) 187, 350
AluI AGCT 2 cut(s) 187, 350
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 2 cut(s) 129, 142
AlwNI CAGNNNCTG 2 cut(s) 11, 470
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 6 cut(s) 7, 43, 121, 445, 484, 650
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AseI ATTAAT 1 cut(s) 552
AsiGI ACCGGT 1 cut(s) 454
AspS9I GGNCC 4 cut(s) 8, 14, 122, 445
AsuC2I CCSGG 2 cut(s) 231, 449
AsuHPI GGTGA 2 cut(s) 674, 710
AsuNHI GCTAGC 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 1 cut(s) 14
BaeGI GKGCMC 2 cut(s) 315, 454
BamHI GGATCC 1 cut(s) 134
BanI GGYRCC 2 cut(s) 310, 451
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 431
BccI CCATC 2 cut(s) 113, 213
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 3 cut(s) 6, 12, 47
BcnI CCSGG 2 cut(s) 231, 449
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 4 cut(s) 188, 267, 321, 695
BfmI CTRYAG 1 cut(s) 747
BfuAI ACCTGC 1 cut(s) 63
BglII AGATCT 1 cut(s) 751
BisI GCNGC 1 cut(s) 511
BlsI GCNGC 1 cut(s) 512
Bme1390I CCNGG 5 cut(s) 6, 12, 47, 231, 449
Bme18I GGWCC 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 4 cut(s) 8, 14, 122, 445
BmiI GGNNCC 4 cut(s) 136, 181, 312, 453
BmrFI CCNGG 5 cut(s) 6, 12, 47, 231, 449
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 1 cut(s) 457
BmtI GCTAGC 1 cut(s) 191
BmuI ACTGGG 1 cut(s) 643
BpiI GAAGAC 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 231, 449
BsaI GGTCTC 1 cut(s) 268
BsaJI CCNNGG 3 cut(s) 10, 33, 163
BsaWI WCCGGW 1 cut(s) 454
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 131, 321, 454, 659
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 3 cut(s) 46, 436, 638
Bse3DI GCAATG 1 cut(s) 73
BseBI CCWGG 3 cut(s) 6, 12, 47
BseDI CCNNGG 3 cut(s) 10, 33, 163
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 5 cut(s) 11, 131, 321, 454, 659
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 2 cut(s) 76, 135
BseNI ACTGG 3 cut(s) 46, 436, 638
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 2 cut(s) 315, 454
BshFI GGCC 6 cut(s) 9, 45, 123, 447, 486, 652
BshNI GGYRCC 2 cut(s) 310, 451
BshTI ACCGGT 1 cut(s) 454
BsiHKAI GWGCWC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 3 cut(s) 230, 448, 455
BslI CCNNNNNNNGG 5 cut(s) 11, 131, 321, 454, 659
BsmAI GTCTC 1 cut(s) 268
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 6 cut(s) 9, 45, 123, 447, 486, 652
Bso31I GGTCTC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 3 cut(s) 315, 431, 454
Bsp143I GATC 3 cut(s) 134, 673, 751
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 2 cut(s) 17, 510
BspANI GGCC 6 cut(s) 9, 45, 123, 447, 486, 652
BspCNI CTCAG 2 cut(s) 75, 136
BspLI GGNNCC 4 cut(s) 136, 181, 312, 453
BspMI ACCTGC 1 cut(s) 63
BspOI GCTAGC 1 cut(s) 191
BspPI GGATC 2 cut(s) 129, 142
BspT107I GGYRCC 2 cut(s) 310, 451
BspTNI GGTCTC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 454
BsrI ACTGG 3 cut(s) 46, 436, 638
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 3 cut(s) 10, 33, 163
BssMI GATC 3 cut(s) 134, 673, 751
BssNAI GTATAC 1 cut(s) 414
BssT1I CCWWGG 1 cut(s) 33
Bst1107I GTATAC 1 cut(s) 414
Bst2UI CCWGG 3 cut(s) 6, 12, 47
Bst4CI ACNGT 1 cut(s) 777
Bst6I CTCTTC 2 cut(s) 101, 660
BstC8I GCNNGC 3 cut(s) 189, 622, 654
BstDEI CTNAG 2 cut(s) 62, 144
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 205
BstKTI GATC 3 cut(s) 137, 676, 754
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 3 cut(s) 134, 673, 751
BstMWI GCNNNNNNNGC 2 cut(s) 78, 492
BstNI CCWGG 3 cut(s) 6, 12, 47
BstSCI CCNGG 5 cut(s) 4, 10, 45, 229, 447
BstSFI CTRYAG 1 cut(s) 747
BstSLI GKGCMC 2 cut(s) 315, 454
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 2 cut(s) 134, 751
BstYI RGATCY 2 cut(s) 134, 751
BstZ17I GTATAC 1 cut(s) 414
BsuRI GGCC 6 cut(s) 9, 45, 123, 447, 486, 652
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 335
BtsIMutI CAGTG 6 cut(s) 335, 429, 578, 579, 631, 773
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 3 cut(s) 189, 622, 654
CaiI CAGNNNCTG 2 cut(s) 11, 470
Cfr10I RCCGGY 1 cut(s) 454
Cfr13I GGNCC 4 cut(s) 8, 14, 122, 445
Csp6I GTAC 2 cut(s) 28, 253
CspAI ACCGGT 1 cut(s) 454
CviAII CATG 2 cut(s) 34, 739
CviQI GTAC 2 cut(s) 28, 253
DdeI CTNAG 2 cut(s) 62, 144
DpnI GATC 3 cut(s) 136, 675, 753
DpnII GATC 3 cut(s) 134, 673, 751
EaeI YGGCCR 1 cut(s) 650
Eam1104I CTCTTC 2 cut(s) 101, 660
EarI CTCTTC 2 cut(s) 101, 660
Eco130I CCWWGG 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 3 cut(s) 4, 10, 45
EcoT14I CCWWGG 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 2 cut(s) 37, 742
FaiI YATR 9 cut(s) 35, 219, 414, 493, 565, 613, 731, 740, 749
FatI CATG 2 cut(s) 33, 738
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 1 cut(s) 511
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 511
FspBI CTAG 4 cut(s) 188, 267, 321, 695
GluI GCNGC 1 cut(s) 511
HaeIII GGCC 6 cut(s) 9, 45, 123, 447, 486, 652
HapII CCGG 3 cut(s) 230, 448, 455
Hin1II CATG 2 cut(s) 37, 742
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 2 cut(s) 155, 296
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 3 cut(s) 230, 448, 455
HphI GGTGA 2 cut(s) 674, 710
Hpy166II GTNNAC 2 cut(s) 414, 460
Hpy188I TCNGA 3 cut(s) 54, 134, 145
Hpy188III TCNNGA 2 cut(s) 267, 293
Hpy8I GTNNAC 2 cut(s) 414, 460
Hpy99I CGWCG 1 cut(s) 651
HpyAV CCTTC 3 cut(s) 124, 148, 436
HpyCH4III ACNGT 1 cut(s) 777
HpyCH4V TGCA 3 cut(s) 495, 620, 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 492
HpyF3I CTNAG 2 cut(s) 62, 144
Hsp92II CATG 2 cut(s) 37, 742
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 3 cut(s) 134, 673, 751
LmnI GCTCC 1 cut(s) 185
LweI GCATC 1 cut(s) 457
MaeI CTAG 4 cut(s) 188, 267, 321, 695
MaeIII GTNAC 1 cut(s) 574
MalI GATC 3 cut(s) 136, 675, 753
MboI GATC 3 cut(s) 134, 673, 751
MboII GAAGA 5 cut(s) 88, 227, 677, 704, 746
MflI RGATCY 2 cut(s) 134, 751
MhlI GDGCHC 3 cut(s) 315, 431, 454
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 9 cut(s) 32, 104, 173, 249, 382, 549, 654, 661, 726
Mph1103I ATGCAT 1 cut(s) 497
MseI TTAA 6 cut(s) 197, 459, 503, 534, 552, 639
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 3 cut(s) 230, 448, 455
MspR9I CCNGG 5 cut(s) 6, 12, 47, 231, 449
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 3 cut(s) 6, 12, 47
MwoI GCNNNNNNNGC 2 cut(s) 78, 492
NciI CCSGG 2 cut(s) 231, 449
NcoI CCATGG 1 cut(s) 33
NdeII GATC 3 cut(s) 134, 673, 751
NheI GCTAGC 1 cut(s) 187
NlaIII CATG 2 cut(s) 37, 742
NlaIV GGNNCC 4 cut(s) 136, 181, 312, 453
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 574
NsiI ATGCAT 1 cut(s) 497
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 1 cut(s) 296
PflMI CCANNNNNTGG 2 cut(s) 11, 659
PinAI ACCGGT 1 cut(s) 454
PkrI GCNGC 1 cut(s) 512
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 4, 10, 45
PspGI CCWGG 3 cut(s) 4, 10, 45
PspN4I GGNNCC 4 cut(s) 136, 181, 312, 453
PspPI GGNCC 4 cut(s) 8, 14, 122, 445
PstNI CAGNNNCTG 2 cut(s) 11, 470
PsuI RGATCY 2 cut(s) 134, 751
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 29, 254
RsaNI GTAC 2 cut(s) 28, 253
SaqAI TTAA 6 cut(s) 197, 459, 503, 534, 552, 639
SatI GCNGC 1 cut(s) 511
Sau3AI GATC 3 cut(s) 134, 673, 751
Sau96I GGNCC 4 cut(s) 8, 14, 122, 445
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 5 cut(s) 6, 12, 47, 231, 449
SduI GDGCHC 3 cut(s) 315, 431, 454
SetI ASST 5 cut(s) 24, 77, 189, 352, 723
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 1 cut(s) 747
SinI GGWCC 1 cut(s) 14
SsiI CCGC 2 cut(s) 17, 510
SspMI CTAG 4 cut(s) 188, 267, 321, 695
StyD4I CCNGG 5 cut(s) 4, 10, 45, 229, 447
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 777
TaqI TCGA 1 cut(s) 153
TatI WGTACW 1 cut(s) 252
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 6 cut(s) 197, 459, 503, 534, 552, 639
Tru9I TTAA 6 cut(s) 197, 459, 503, 534, 552, 639
TscAI CASTG 6 cut(s) 335, 436, 579, 585, 638, 780
TseFI GTSAC 1 cut(s) 574
Tsp45I GTSAC 1 cut(s) 574
TspRI CASTG 6 cut(s) 335, 436, 579, 585, 638, 780
Van91I CCANNNNNTGG 2 cut(s) 11, 659
VpaK11BI GGWCC 1 cut(s) 14
VspI ATTAAT 1 cut(s) 552
XbaI TCTAGA 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 4 cut(s) 188, 267, 321, 695
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.