Rmu_sc0002389.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002389.1
OG0013665
Physical Location & Seq
Forward (+)
52071 .. 54052
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002389.1_g000014.1.cds

Sequence Viewer

Length: 846 bp
atgccgggccccggtccgcacctgatgtacgccatgggttcgggtgtggctctgaccactctcaccaacggcaggttcagcccccaccacaccctgatctataccgtcaacgccttcttcggccccgacatcggatccttcaccgaatggctggcctccctcgtcggcggcttcggcttcgacttcggctccgagctcgctgacgccatccaccaccccttctactacgtcatcattcttggtctccctctttgtattctctactcctggctgtccagaatcttcctcctcaatagggtcctcgactccttccctccggttcccctaacaaagttgcagtgcatcttgttggtgtcagccggttctttgtcacattttttcctcgatcacttgttcgaggaaaatgggcattctggtacgtatacatggattttgagcactggttggtgggagggccgcgcacctattaatccagatgctgttgttgtcgtcggccttttatgcattggcttaattggtggctttatttacatcaacagagttaagcctcccaagtctgttaacaaatattcatcaaatgaatcattccaactaatcatcatcatagcaagcctgtactgcttatggtgcacaagccaagtatacttggttaatcctcgtcggccagcagttggtgaagaagctgaccttggagttctctataatcatattgataaagaggacatgccacgcttgctgtcgagtatgatcgaagtagattttccaagtcttgccacagccctgagttttgttttgttgtaccttgttaccatgatttttcaggattttgtggcaacaggagtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

31.1

Weight (kDa)

5.49

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013665)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05110 FvH4_5g05110
malus_domestica MD06G1177600.v1.1
prunus_persica Prupe.5G176300_v2.0.a1 Prupe.5G176300_v2.0.a1 Prupe.5G176300_v2.0.a1
pyrus_communis pycom06g15760
rosa_chinensis RchiOBHm_Chr7g0193891
rosa_laevigata RLG00000004236
rosa_multiflora Rmu_sc0002389.1_g000014
rosa_roxburghii Rroxscaffold_3G00261730
rosa_rugosa Rorug07G0012500 Rorug07G0012500
rosa_samantha Rh7AG139400 Rh7BG139900 Rh7CG143600 Rh7DG140800
rosa_wichuraiana Rw7G011890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 63
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 2 cut(s) 422, 642
AccII CGCG 1 cut(s) 459
AciI CCGC 3 cut(s) 17, 168, 457
AclWI GGATC 2 cut(s) 129, 142
AcoI YGGCCR 1 cut(s) 662
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 4 cut(s) 29, 418, 617, 800
AfiI CCNNNNNNNGG 5 cut(s) 11, 72, 131, 295, 671
AjnI CCWGG 1 cut(s) 266
AjuI GAANNNNNNNTTGG 4 cut(s) 59, 91, 672, 704
AluBI AGCT 2 cut(s) 196, 683
AluI AGCT 2 cut(s) 196, 683
Alw21I GWGCWC 3 cut(s) 198, 440, 632
Alw26I GTCTC 1 cut(s) 248
Alw44I GTGCAC 1 cut(s) 628
AlwI GGATC 2 cut(s) 129, 142
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 6 cut(s) 7, 121, 153, 454, 493, 662
ApaI GGGCCC 1 cut(s) 11
ApaLI GTGCAC 1 cut(s) 628
AseI ATTAAT 1 cut(s) 468
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 6 cut(s) 7, 8, 14, 122, 298, 454
AsuC2I CCSGG 2 cut(s) 6, 12
AsuHPI GGTGA 3 cut(s) 55, 133, 686
AvaII GGWCC 2 cut(s) 14, 298
BaeGI GKGCMC 2 cut(s) 11, 632
BamHI GGATCC 1 cut(s) 134
BanII GRGCYC 2 cut(s) 11, 198
Bbv12I GWGCWC 3 cut(s) 198, 440, 632
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 268
BcnI CCSGG 2 cut(s) 6, 12
BcoDI GTCTC 1 cut(s) 248
BfuAI ACCTGC 1 cut(s) 63
BisI GCNGC 2 cut(s) 169, 457
BlsI GCNGC 2 cut(s) 170, 458
Bme1390I CCNGG 3 cut(s) 6, 12, 268
Bme18I GGWCC 2 cut(s) 14, 298
BmgT120I GGNCC 6 cut(s) 7, 8, 14, 122, 298, 454
BmiI GGNNCC 7 cut(s) 9, 10, 124, 136, 190, 299, 321
BmrFI CCNGG 3 cut(s) 6, 12, 268
BmsI GCATC 2 cut(s) 351, 466
BpuMI CCSGG 2 cut(s) 6, 12
BsaAI YACGTR 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 10, 33, 688
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 72, 131, 295, 671
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 268
BseDI CCNNGG 3 cut(s) 10, 33, 688
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 5 cut(s) 11, 72, 131, 295, 671
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 2 cut(s) 11, 632
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 6 cut(s) 9, 123, 155, 456, 495, 664
BsiHKAI GWGCWC 3 cut(s) 198, 440, 632
BsiSI CCGG 4 cut(s) 5, 12, 317, 360
BslI CCNNNNNNNGG 5 cut(s) 11, 72, 131, 295, 671
BsmAI GTCTC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 6 cut(s) 9, 123, 155, 456, 495, 664
Bso31I GGTCTC 1 cut(s) 248
Bsp120I GGGCCC 1 cut(s) 7
Bsp1286I GDGCHC 4 cut(s) 11, 198, 440, 632
Bsp143I GATC 4 cut(s) 96, 134, 385, 747
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 3 cut(s) 17, 168, 457
BspANI GGCC 6 cut(s) 9, 123, 155, 456, 495, 664
BspCNI CTCAG 1 cut(s) 774
BspFNI CGCG 1 cut(s) 459
BspLI GGNNCC 7 cut(s) 9, 10, 124, 136, 190, 299, 321
BspMI ACCTGC 1 cut(s) 63
BspPI GGATC 2 cut(s) 129, 142
BspTNI GGTCTC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 10, 33, 688
BssMI GATC 4 cut(s) 96, 134, 385, 747
BssNAI GTATAC 2 cut(s) 423, 643
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 33, 688
Bst1107I GTATAC 2 cut(s) 423, 643
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 420
BstC8I GCNNGC 5 cut(s) 153, 198, 610, 666, 734
BstDEI CTNAG 1 cut(s) 782
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 1 cut(s) 459
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 4 cut(s) 99, 137, 388, 750
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 4 cut(s) 96, 134, 385, 747
BstMWI GCNNNNNNNGC 6 cut(s) 78, 174, 501, 618, 627, 733
BstNI CCWGG 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 3 cut(s) 4, 10, 266
BstSLI GKGCMC 2 cut(s) 11, 632
BstSNI TACGTA 1 cut(s) 420
BstUI CGCG 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BstZ17I GTATAC 2 cut(s) 423, 643
BsuRI GGCC 6 cut(s) 9, 123, 155, 456, 495, 664
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 207
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 2 cut(s) 344, 438
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 5 cut(s) 153, 198, 610, 666, 734
CaiI CAGNNNCTG 1 cut(s) 479
CfoI GCGC 1 cut(s) 461
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 6 cut(s) 7, 8, 14, 122, 298, 454
CpoI CGGWCCG 1 cut(s) 14
CseI GACGC 1 cut(s) 212
Csp6I GTAC 4 cut(s) 28, 417, 616, 799
CspI CGGWCCG 1 cut(s) 14
CviAII CATG 4 cut(s) 34, 426, 724, 811
CviQI GTAC 4 cut(s) 28, 417, 616, 799
DdeI CTNAG 1 cut(s) 782
DpnI GATC 4 cut(s) 98, 136, 387, 749
DpnII GATC 4 cut(s) 96, 134, 385, 747
EaeI YGGCCR 1 cut(s) 662
Ecl136II GAGCTC 1 cut(s) 196
Eco105I TACGTA 1 cut(s) 420
Eco130I CCWWGG 2 cut(s) 33, 688
Eco24I GRGCYC 2 cut(s) 11, 198
Eco31I GGTCTC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 14, 298
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 2 cut(s) 8, 298
EcoRII CCWGG 1 cut(s) 266
EcoT14I CCWWGG 2 cut(s) 33, 688
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 2 cut(s) 11, 198
ErhI CCWWGG 2 cut(s) 33, 688
FaeI CATG 4 cut(s) 37, 429, 727, 814
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FatI CATG 4 cut(s) 33, 425, 723, 810
FblI GTMKAC 2 cut(s) 422, 642
Fnu4HI GCNGC 2 cut(s) 169, 457
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 11, 198
Fsp4HI GCNGC 2 cut(s) 169, 457
GlaI GCGC 1 cut(s) 460
GluI GCNGC 2 cut(s) 169, 457
HaeIII GGCC 6 cut(s) 9, 123, 155, 456, 495, 664
HapII CCGG 4 cut(s) 5, 12, 317, 360
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 461
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 4 cut(s) 37, 429, 727, 814
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 2 cut(s) 109, 562
HindII GTYRAC 2 cut(s) 109, 562
HinfI GANTC 3 cut(s) 279, 305, 581
HpaI GTTAAC 1 cut(s) 562
HpaII CCGG 4 cut(s) 5, 12, 317, 360
HphI GGTGA 3 cut(s) 55, 133, 686
Hpy166II GTNNAC 5 cut(s) 109, 423, 562, 630, 643
Hpy188I TCNGA 3 cut(s) 54, 134, 193
Hpy188III TCNNGA 3 cut(s) 276, 473, 821
Hpy8I GTNNAC 5 cut(s) 109, 423, 562, 630, 643
Hpy99I CGWCG 3 cut(s) 167, 494, 663
HpyAV CCTTC 4 cut(s) 124, 148, 229, 319
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 2 cut(s) 228, 419
HpyCH4V TGCA 4 cut(s) 337, 342, 504, 630
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 174, 501, 618, 627, 733
HpyF3I CTNAG 1 cut(s) 782
HpySE526I ACGT 2 cut(s) 228, 419
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 4 cut(s) 37, 429, 727, 814
HspAI GCGC 1 cut(s) 459
KspAI GTTAAC 1 cut(s) 562
Kzo9I GATC 4 cut(s) 96, 134, 385, 747
LmnI GCTCC 1 cut(s) 194
LweI GCATC 2 cut(s) 351, 466
MaeII ACGT 2 cut(s) 228, 419
MaeIII GTNAC 2 cut(s) 369, 805
MalI GATC 4 cut(s) 98, 136, 387, 749
MboI GATC 4 cut(s) 96, 134, 385, 747
MboII GAAGA 3 cut(s) 109, 274, 689
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 4 cut(s) 11, 198, 440, 632
MluCI AATT 1 cut(s) 513
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 613
Mph1103I ATGCAT 1 cut(s) 506
MseI TTAA 5 cut(s) 468, 512, 543, 561, 651
MspI CCGG 4 cut(s) 5, 12, 317, 360
MspR9I CCNGG 3 cut(s) 6, 12, 268
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 1 cut(s) 459
MwoI GCNNNNNNNGC 6 cut(s) 78, 174, 501, 618, 627, 733
NciI CCSGG 2 cut(s) 6, 12
NcoI CCATGG 1 cut(s) 33
NdeII GATC 4 cut(s) 96, 134, 385, 747
NlaIII CATG 4 cut(s) 37, 429, 727, 814
NlaIV GGNNCC 7 cut(s) 9, 10, 124, 136, 190, 299, 321
NmuCI GTSAC 1 cut(s) 369
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 1 cut(s) 727
PctI GAATGC 1 cut(s) 409
PfeI GAWTC 2 cut(s) 279, 581
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 2 cut(s) 170, 458
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 420
PpuMI RGGWCCY 1 cut(s) 298
PshBI ATTAAT 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 7 cut(s) 9, 10, 124, 136, 190, 299, 321
PspOMI GGGCCC 1 cut(s) 7
PspPI GGNCC 6 cut(s) 7, 8, 14, 122, 298, 454
PspPPI RGGWCCY 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 134
RsaI GTAC 4 cut(s) 29, 418, 617, 800
RsaNI GTAC 4 cut(s) 28, 417, 616, 799
Rsr2I CGGWCCG 1 cut(s) 14
RsrII CGGWCCG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 5 cut(s) 468, 512, 543, 561, 651
SatI GCNGC 2 cut(s) 169, 457
Sau3AI GATC 4 cut(s) 96, 134, 385, 747
Sau96I GGNCC 6 cut(s) 7, 8, 14, 122, 298, 454
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 3 cut(s) 6, 12, 268
SduI GDGCHC 4 cut(s) 11, 198, 440, 632
SetI ASST 9 cut(s) 24, 77, 198, 231, 422, 466, 685, 690, 804
SfaNI GCATC 2 cut(s) 351, 466
SinI GGWCC 2 cut(s) 14, 298
SnaBI TACGTA 1 cut(s) 420
Sse9I AATT 1 cut(s) 513
SsiI CCGC 3 cut(s) 17, 168, 457
SspI AATATT 1 cut(s) 569
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 3 cut(s) 4, 10, 266
StyI CCWWGG 2 cut(s) 33, 688
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 2 cut(s) 231, 422
TaqI TCGA 6 cut(s) 180, 303, 384, 396, 740, 750
TasI AATT 1 cut(s) 513
TatI WGTACW 1 cut(s) 615
TauI GCSGC 2 cut(s) 171, 459
TfiI GAWTC 2 cut(s) 279, 581
Tru1I TTAA 5 cut(s) 468, 512, 543, 561, 651
Tru9I TTAA 5 cut(s) 468, 512, 543, 561, 651
TscAI CASTG 2 cut(s) 344, 445
TseFI GTSAC 1 cut(s) 369
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 2 cut(s) 561, 594
TspRI CASTG 2 cut(s) 344, 445
Van91I CCANNNNNTGG 1 cut(s) 671
VneI GTGCAC 1 cut(s) 628
VpaK11BI GGWCC 2 cut(s) 14, 298
VspI ATTAAT 1 cut(s) 468
XceI RCATGY 1 cut(s) 727
XmiI GTMKAC 2 cut(s) 422, 642
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.