Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp08

Orthogroup ID

OG0011449

Physical Location & Seq

Genomic Coordinates Reverse (-)

18810297 .. 18813880

Gene Structure 3584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.8G196500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGGGCGCTTTGGATGAGGGACATTTCTGCTCGGAACTTGAGAATGGAGTGAAATTGGGTTGCATCGAGCTGAGTTTTGAGGAAGAACCTGAAACCATAGCTATTGAAGATGCTGTGAAAGTTCTCATGCAGGGTCTGGGAGAGGATGTTAACAGAGAAGGTTTGAAGAAGACGCCTCTTCGCGTTGCCAAGGCCCTTCGGGAAGGAACAAGGGGTTACAGACAAAAGGCTAAGGACATTGTGCAAGGTGCTTTATTTCCTGAATCTGGTCTCGACAATACAGTTGGTCATGCTGGAGGAGCTGGTGGACTCGTGGTTGTTAGAGATCTTGACCTCTTCTCGTATTGTGAATCTTGTATGCTACCATTCCAGGTTAAATGTCATGTGGGTTATGTCCCTTCTGGTCAACGGGTTGTAGGCTTAAGCAAGCTCTCTCGAGTAGCTGATGTGTTTGCAAAACGCCTCCAAGAGCCTCAGCACCTAGCAGACGAAATGTGTTCAGCTTTGCAGCATGGCATCAAGCCAGCAGGTGTTGCAGTTGTACTCCAATGTTTACACATCCATTTCCCAAAAGTAGAATCAGCCTTTCTTGACTCGGACCACCAAGGATGGGTAGAGTTACTGGTTAGTTCAGGCTCAGGAGTTTTTGAGAATGAAAATGCCAATCTTTGGGCAGATTTTTTGAATCTTCTGAGATTCAGAGGCATAAATGTCGAGAAAAATCGCATGAGAGACTCAAGCGATCAACACTGGTGTCCATCTCGATTCCCCTCAGGTGCTATTGCTGCCTCCAAGATTGAATCAGTTAATCAGGGTATGGTCACTGCAGTAGCTTCAATTCTTAGGTCGTTAGGTGAAGACCCATTAAGGAAGGAGCTTGTAGGAACACCTGCTCGTTTCGTGAAGTGGTTGATGAACTTCCAAACTACTAATTTAGATATGAAGCTGAATGGCTTTGTTTCTGACAGGGTGGATCCTCTAAAGCTCAATGGAGATGGCTGCAAGGAAAAGAAAATCCACTCTGAGCTGAACTTGTCATTCTGGTCGCAGTGTGAGCATCATCTACTTCCCTTCTATGGTGTTGTGCATATAGGATATTTGTGCACTGAAGGATTCAATCCCATTGCGAAATCTCTTTTACAATCGATTGTATGTTTCTACGGTTACAAGCTCCAAGTACAAGAAAGACTCACCCGGCAGATAGCAGAGACTGCATCATCACTGTTAGGTGGAGATGTGATTGTGGTTGTGGAGGCTAATCACACTTGTATGATCTCGAGAGGCATCGAGAAGTTTGGAAGTAGTACAGCGACAATTGCTGTACTGGGTCGATTTTCGACTGACCCTGCTGCAAGGGCTAAGTTTTTGCAGAGCATTCCAAACACTGCCGTTTCAGGACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003933 GO:0003934 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006725 GO:0006729 GO:0006732 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008270 GO:0008277 GO:0009058 GO:0009108 GO:0009966 GO:0009987 GO:0010646 GO:0016020 GO:0016787 GO:0016810 GO:0016814 GO:0017076 GO:0017144 GO:0018130 GO:0019001 GO:0019238 GO:0019438 GO:0019637 GO:0019751 GO:0023051 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0034311 GO:0034312 GO:0034641 GO:0035639 GO:0035998 GO:0036094 GO:0042558 GO:0042559 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046146 GO:0046165 GO:0046173 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051066 GO:0051186 GO:0051188 GO:0065007 GO:0071704 GO:0090407 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00790 ko01100 map00790 map01100

Pfam Domains

Protein Families

GTP_cyclohydroI

Protein Analysis

468

Amino Acids

51.17

Weight (kDa)

6.41

Isoelectric Point (pI)

38.95

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011449)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07270 AT3G07270
fragaria_vesca	FvH4_2g18370 FvH4_2g18370 FvH4_2g18370
malus_domestica	MD05G1169300.v1.1 MD10G1157600.v1.1
prunus_persica	Prupe.8G196500_v2.0.a1 Prupe.8G196500_v2.0.a1
pyrus_communis	pycom05g15690
rosa_chinensis	RchiOBHm_Chr6g0283751
rosa_laevigata	RLG00000012801
rosa_multiflora	Rmu_sc0001521.1_g000012
rosa_roxburghii	Rroxscaffold_7G00185210
rosa_rugosa	Rorug06G0157600
rosa_samantha	Rh6AG269700 Rh6BG271800 Rh6CG272000 Rh6DG265600
rosa_wichuraiana	Rw6G023310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	518, 898
Acc36I	ACCTGC	2 cut(s)	518, 898
AccB7I	CCANNNNNTGG	1 cut(s)	669
AccII	CGCG	1 cut(s)	183
AclWI	GGATC	2 cut(s)	968, 981
AcuI	CTGAAG	1 cut(s)	1128
AcyI	GRCGYC	1 cut(s)	173
AfaI	GTAC	4 cut(s)	543, 1179, 1306, 1323
AfiI	CCNNNNNNNGG	4 cut(s)	266, 610, 669, 1076
AflII	CTTAAG	1 cut(s)	421
AgsI	TTSAA	6 cut(s)	107, 166, 685, 800, 837, 1117
AjnI	CCWGG	1 cut(s)	369
Alw21I	GWGCWC	1 cut(s)	1106
Alw26I	GTCTC	3 cut(s)	275, 726, 1202
Alw44I	GTGCAC	1 cut(s)	1102
AlwI	GGATC	2 cut(s)	968, 981
AlwNI	CAGNNNCTG	2 cut(s)	136, 1211
Ama87I	CYCGRG	2 cut(s)	435, 1276
AoxI	GGCC	1 cut(s)	192
ApaLI	GTGCAC	1 cut(s)	1102
ApeKI	GCWGC	4 cut(s)	508, 785, 999, 1349
AspLEI	GCGC	1 cut(s)	8
AspS9I	GGNCC	2 cut(s)	193, 598
AsuC2I	CCSGG	1 cut(s)	1195
AsuHPI	GGTGA	2 cut(s)	866, 1183
AvaI	CYCGRG	2 cut(s)	435, 1276
AvaII	GGWCC	1 cut(s)	598
AxyI	CCTNAGG	1 cut(s)	772
BaeGI	GKGCMC	1 cut(s)	1106
BamHI	GGATCC	1 cut(s)	973
BauI	CACGAG	1 cut(s)	311
BbsI	GAAGAC	2 cut(s)	176, 864
Bbv12I	GWGCWC	1 cut(s)	1106
BbvCI	CCTCAGC	1 cut(s)	474
BbvI	GCAGC	4 cut(s)	520, 772, 986, 1336
BccI	CCATC	3 cut(s)	603, 766, 989
BceAI	ACGGC	1 cut(s)	1373
BcgI	CGANNNNNNTGC	2 cut(s)	694, 728
BciT130I	CCWGG	1 cut(s)	371
BcnI	CCSGG	1 cut(s)	1195
BcoDI	GTCTC	3 cut(s)	275, 726, 1202
BfaI	CTAG	1 cut(s)	482
BfmI	CTRYAG	1 cut(s)	825
BfoI	RGCGCY	1 cut(s)	9
BfrI	CTTAAG	1 cut(s)	421
BfuAI	ACCTGC	2 cut(s)	518, 898
BglII	AGATCT	1 cut(s)	325
BisI	GCNGC	4 cut(s)	509, 786, 1000, 1350
BlsI	GCNGC	4 cut(s)	510, 787, 1001, 1351
Bme1390I	CCNGG	2 cut(s)	371, 1195
Bme18I	GGWCC	1 cut(s)	598
BmeT110I	CYCGRG	2 cut(s)	435, 1276
BmgT120I	GGNCC	2 cut(s)	193, 598
BmiI	GGNNCC	1 cut(s)	975
BmrFI	CCNGG	2 cut(s)	371, 1195
BmrI	ACTGGG	1 cut(s)	1334
BmsI	GCATC	6 cut(s)	72, 100, 525, 1066, 1223, 1293
BmuI	ACTGGG	1 cut(s)	1334
BpiI	GAAGAC	2 cut(s)	176, 864
BpmI	CTGGAG	1 cut(s)	315
Bpu10I	CCTNAGC	3 cut(s)	231, 474, 637
BpuEI	CTTGAG	2 cut(s)	59, 721
BpuMI	CCSGG	1 cut(s)	1195
Bsa29I	ATCGAT	1 cut(s)	1145
BsaHI	GRCGYC	1 cut(s)	173
BsaI	GGTCTC	1 cut(s)	275
BsaJI	CCNNGG	2 cut(s)	189, 604
BsaXI	ACNNNNNCTCC	2 cut(s)	132, 162
Bsc4I	CCNNNNNNNGG	4 cut(s)	266, 610, 669, 1076
Bse1I	ACTGG	3 cut(s)	627, 755, 1329
Bse21I	CCTNAGG	1 cut(s)	772
Bse3DI	GCAATG	1 cut(s)	1122
BseBI	CCWGG	1 cut(s)	371
BseCI	ATCGAT	1 cut(s)	1145
BseDI	CCNNGG	2 cut(s)	189, 604
BseGI	GGATG	4 cut(s)	19, 151, 558, 614
BseLI	CCNNNNNNNGG	4 cut(s)	266, 610, 669, 1076
BseMI	GCAATG	1 cut(s)	1122
BseMII	CTCAG	6 cut(s)	62, 488, 651, 683, 786, 1014
BseNI	ACTGG	3 cut(s)	627, 755, 1329
BseRI	GAGGAG	1 cut(s)	312
BseSI	GKGCMC	1 cut(s)	1106
BseXI	GCAGC	4 cut(s)	520, 772, 986, 1336
Bsh1236I	CGCG	1 cut(s)	183
BshFI	GGCC	1 cut(s)	194
BshVI	ATCGAT	1 cut(s)	1145
BsiHKAI	GWGCWC	1 cut(s)	1106
BsiHKCI	CYCGRG	2 cut(s)	435, 1276
BsiSI	CCGG	1 cut(s)	1195
BslFI	GGGAC	2 cut(s)	33, 380
BslI	CCNNNNNNNGG	4 cut(s)	266, 610, 669, 1076
BsmAI	GTCTC	3 cut(s)	275, 726, 1202
BsmFI	GGGAC	2 cut(s)	33, 380
BsmI	GAATGC	1 cut(s)	1374
BsnI	GGCC	1 cut(s)	194
Bso31I	GGTCTC	1 cut(s)	275
BsoBI	CYCGRG	2 cut(s)	435, 1276
Bsp1286I	GDGCHC	1 cut(s)	1106
Bsp143I	GATC	4 cut(s)	325, 742, 973, 1272
BspANI	GGCC	1 cut(s)	194
BspCNI	CTCAG	6 cut(s)	63, 487, 650, 684, 785, 1015
BspDI	ATCGAT	1 cut(s)	1145
BspFNI	CGCG	1 cut(s)	183
BspLI	GGNNCC	1 cut(s)	975
BspMAI	CTGCAG	1 cut(s)	829
BspMI	ACCTGC	2 cut(s)	518, 898
BspPI	GGATC	2 cut(s)	968, 981
BspTI	CTTAAG	1 cut(s)	421
BspTNI	GGTCTC	1 cut(s)	275
BsrDI	GCAATG	1 cut(s)	1122
BsrI	ACTGG	3 cut(s)	627, 755, 1329
BssECI	CCNNGG	2 cut(s)	189, 604
BssMI	GATC	4 cut(s)	325, 742, 973, 1272
BssNI	GRCGYC	1 cut(s)	173
BssSI	CACGAG	1 cut(s)	311
BssT1I	CCWWGG	2 cut(s)	189, 604
Bst2BI	CACGAG	1 cut(s)	311
Bst2UI	CCWGG	1 cut(s)	371
Bst4CI	ACNGT	3 cut(s)	283, 1163, 1224
Bst6I	CTCTTC	2 cut(s)	183, 341
BstACI	GRCGYC	1 cut(s)	173
BstAFI	CTTAAG	1 cut(s)	421
BstAPI	GCANNNNNTGC	2 cut(s)	533, 1211
BstC8I	GCNNGC	2 cut(s)	428, 525
BstDEI	CTNAG	9 cut(s)	71, 231, 474, 637, 692, 772, 842, 1023, 1359
BstF5I	GGATG	4 cut(s)	19, 151, 558, 614
BstFNI	CGCG	1 cut(s)	183
BstH2I	RGCGCY	1 cut(s)	9
BstHHI	GCGC	1 cut(s)	8
BstKTI	GATC	4 cut(s)	328, 745, 976, 1275
BstMAI	GTCTC	3 cut(s)	275, 726, 1202
BstMBI	GATC	4 cut(s)	325, 742, 973, 1272
BstMWI	GCNNNNNNNGC	7 cut(s)	299, 533, 785, 1054, 1211, 1316, 1355
BstNI	CCWGG	1 cut(s)	371
BstSCI	CCNGG	2 cut(s)	369, 1193
BstSFI	CTRYAG	1 cut(s)	825
BstSLI	GKGCMC	1 cut(s)	1106
BstUI	CGCG	1 cut(s)	183
BstV1I	GCAGC	4 cut(s)	520, 772, 986, 1336
BstV2I	GAAGAC	2 cut(s)	176, 864
BstX2I	RGATCY	2 cut(s)	325, 973
BstYI	RGATCY	2 cut(s)	325, 973
Bsu15I	ATCGAT	1 cut(s)	1145
Bsu36I	CCTNAGG	1 cut(s)	772
BsuRI	GGCC	1 cut(s)	194
BsuTUI	ATCGAT	1 cut(s)	1145
BtsCI	GGATG	4 cut(s)	19, 151, 558, 614
BtsI	GCAGTG	3 cut(s)	822, 1055, 1383
BtsIMutI	CAGTG	6 cut(s)	748, 822, 1055, 1104, 1220, 1383
BveI	ACCTGC	2 cut(s)	518, 898
Cac8I	GCNNGC	2 cut(s)	428, 525
CaiI	CAGNNNCTG	2 cut(s)	136, 1211
CfoI	GCGC	1 cut(s)	8
Cfr13I	GGNCC	2 cut(s)	193, 598
ClaI	ATCGAT	1 cut(s)	1145
CseI	GACGC	1 cut(s)	181
Csp6I	GTAC	4 cut(s)	542, 1178, 1305, 1322
CviAII	CATG	5 cut(s)	127, 290, 383, 512, 727
CviQI	GTAC	4 cut(s)	542, 1178, 1305, 1322
DdeI	CTNAG	9 cut(s)	71, 231, 474, 637, 692, 772, 842, 1023, 1359
DpnI	GATC	4 cut(s)	327, 744, 975, 1274
DpnII	GATC	4 cut(s)	325, 742, 973, 1272
Eam1104I	CTCTTC	2 cut(s)	183, 341
EarI	CTCTTC	2 cut(s)	183, 341
Eco130I	CCWWGG	2 cut(s)	189, 604
Eco31I	GGTCTC	1 cut(s)	275
Eco47I	GGWCC	1 cut(s)	598
Eco57I	CTGAAG	1 cut(s)	1128
Eco81I	CCTNAGG	1 cut(s)	772
Eco88I	CYCGRG	2 cut(s)	435, 1276
EcoO109I	RGGNCCY	1 cut(s)	193
EcoRII	CCWGG	1 cut(s)	369
EcoT14I	CCWWGG	2 cut(s)	189, 604
ErhI	CCWWGG	2 cut(s)	189, 604
FaeI	CATG	5 cut(s)	130, 293, 386, 515, 730
FaqI	GGGAC	2 cut(s)	33, 380
FatI	CATG	5 cut(s)	126, 289, 382, 511, 726
Fnu4HI	GCNGC	4 cut(s)	509, 786, 1000, 1350
FokI	GGATG	4 cut(s)	26, 158, 545, 621
Fsp4HI	GCNGC	4 cut(s)	509, 786, 1000, 1350
FspBI	CTAG	1 cut(s)	482
GlaI	GCGC	1 cut(s)	7
GluI	GCNGC	4 cut(s)	509, 786, 1000, 1350
GsuI	CTGGAG	1 cut(s)	315
HaeII	RGCGCY	1 cut(s)	9
HaeIII	GGCC	1 cut(s)	194
HapII	CCGG	1 cut(s)	1195
HgaI	GACGC	1 cut(s)	181
HhaI	GCGC	1 cut(s)	8
Hin1I	GRCGYC	1 cut(s)	173
Hin1II	CATG	5 cut(s)	130, 293, 386, 515, 730
Hin6I	GCGC	1 cut(s)	6
HinP1I	GCGC	1 cut(s)	6
HincII	GTYRAC	2 cut(s)	151, 407
HindII	GTYRAC	2 cut(s)	151, 407
HpaI	GTTAAC	1 cut(s)	151
HpaII	CCGG	1 cut(s)	1195
HphI	GGTGA	2 cut(s)	866, 1183
Hpy166II	GTNNAC	5 cut(s)	151, 308, 407, 554, 1104
Hpy188I	TCNGA	6 cut(s)	34, 598, 693, 701, 964, 1024
Hpy8I	GTNNAC	5 cut(s)	151, 308, 407, 554, 1104
HpyAV	CCTTC	7 cut(s)	152, 197, 206, 408, 865, 1081, 1103
HpyCH4III	ACNGT	3 cut(s)	283, 1163, 1224
HpyF10VI	GCNNNNNNNGC	7 cut(s)	299, 533, 785, 1054, 1211, 1316, 1355
HpyF3I	CTNAG	9 cut(s)	71, 231, 474, 637, 692, 772, 842, 1023, 1359
Hsp92I	GRCGYC	1 cut(s)	173
Hsp92II	CATG	5 cut(s)	130, 293, 386, 515, 730
HspAI	GCGC	1 cut(s)	6
KspAI	GTTAAC	1 cut(s)	151
Kzo9I	GATC	4 cut(s)	325, 742, 973, 1272
LmnI	GCTCC	3 cut(s)	299, 874, 1176
Lsp1109I	GCAGC	4 cut(s)	520, 772, 986, 1336
LweI	GCATC	6 cut(s)	72, 100, 525, 1066, 1223, 1293
MaeI	CTAG	1 cut(s)	482
MaeIII	GTNAC	4 cut(s)	215, 618, 820, 1163
MalI	GATC	4 cut(s)	327, 744, 975, 1274
MboI	GATC	4 cut(s)	325, 742, 973, 1272
MboII	GAAGA	8 cut(s)	95, 119, 170, 178, 181, 328, 680, 869
MfeI	CAATTG	1 cut(s)	1314
MflI	RGATCY	2 cut(s)	325, 973
MhlI	GDGCHC	1 cut(s)	1106
MluCI	AATT	4 cut(s)	53, 837, 931, 1314
MlyI	GAGTC	4 cut(s)	303, 587, 728, 1182
MseI	TTAA	5 cut(s)	150, 375, 422, 807, 866
MslI	CAYNNNNRTG	1 cut(s)	1268
MspCI	CTTAAG	1 cut(s)	421
MspI	CCGG	1 cut(s)	1195
MspR9I	CCNGG	2 cut(s)	371, 1195
MunI	CAATTG	1 cut(s)	1314
Mva1269I	GAATGC	1 cut(s)	1374
MvaI	CCWGG	1 cut(s)	371
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	7 cut(s)	299, 533, 785, 1054, 1211, 1316, 1355
NciI	CCSGG	1 cut(s)	1195
NdeII	GATC	4 cut(s)	325, 742, 973, 1272
NlaIII	CATG	5 cut(s)	130, 293, 386, 515, 730
NlaIV	GGNNCC	1 cut(s)	975
NmuCI	GTSAC	1 cut(s)	820
PaeR7I	CTCGAG	2 cut(s)	435, 1276
PaqCI	CACCTGC	2 cut(s)	518, 898
PctI	GAATGC	1 cut(s)	1374
PfeI	GAWTC	8 cut(s)	263, 350, 578, 685, 696, 765, 800, 1113
PflMI	CCANNNNNTGG	1 cut(s)	669
PkrI	GCNGC	4 cut(s)	510, 787, 1001, 1351
PleI	GAGTC	4 cut(s)	303, 587, 728, 1182
PpsI	GAGTC	4 cut(s)	303, 587, 728, 1182
Psp6I	CCWGG	1 cut(s)	369
PspGI	CCWGG	1 cut(s)	369
PspN4I	GGNNCC	1 cut(s)	975
PspPI	GGNCC	2 cut(s)	193, 598
PstI	CTGCAG	1 cut(s)	829
PstNI	CAGNNNCTG	2 cut(s)	136, 1211
PsuI	RGATCY	2 cut(s)	325, 973
RsaI	GTAC	4 cut(s)	543, 1179, 1306, 1323
RsaNI	GTAC	4 cut(s)	542, 1178, 1305, 1322
RseI	CAYNNNNRTG	1 cut(s)	1268
SaqAI	TTAA	5 cut(s)	150, 375, 422, 807, 866
SatI	GCNGC	4 cut(s)	509, 786, 1000, 1350
Sau3AI	GATC	4 cut(s)	325, 742, 973, 1272
Sau96I	GGNCC	2 cut(s)	193, 598
SchI	GAGTC	4 cut(s)	303, 587, 728, 1182
ScrFI	CCNGG	2 cut(s)	371, 1195
SduI	GDGCHC	1 cut(s)	1106
SfaNI	GCATC	6 cut(s)	72, 100, 525, 1066, 1223, 1293
SfcI	CTRYAG	1 cut(s)	825
Sfr274I	CTCGAG	2 cut(s)	435, 1276
SinI	GGWCC	1 cut(s)	598
SlaI	CTCGAG	2 cut(s)	435, 1276
SmiMI	CAYNNNNRTG	1 cut(s)	1268
SmlI	CTYRAG	5 cut(s)	38, 421, 435, 736, 1276
SmoI	CTYRAG	5 cut(s)	38, 421, 435, 736, 1276
Sse9I	AATT	4 cut(s)	53, 837, 931, 1314
SspMI	CTAG	1 cut(s)	482
StyD4I	CCNGG	2 cut(s)	369, 1193
StyI	CCWWGG	2 cut(s)	189, 604
TaaI	ACNGT	3 cut(s)	283, 1163, 1224
TasI	AATT	4 cut(s)	53, 837, 931, 1314
TatI	WGTACW	4 cut(s)	541, 1177, 1304, 1321
TfiI	GAWTC	8 cut(s)	263, 350, 578, 685, 696, 765, 800, 1113
Tru1I	TTAA	5 cut(s)	150, 375, 422, 807, 866
Tru9I	TTAA	5 cut(s)	150, 375, 422, 807, 866
TscAI	CASTG	6 cut(s)	755, 829, 1055, 1111, 1227, 1390
TseFI	GTSAC	1 cut(s)	820
TseI	GCWGC	4 cut(s)	508, 785, 999, 1349
Tsp45I	GTSAC	1 cut(s)	820
TspDTI	ATGAA	3 cut(s)	669, 929, 956
TspRI	CASTG	6 cut(s)	755, 829, 1055, 1111, 1227, 1390
Van91I	CCANNNNNTGG	1 cut(s)	669
Vha464I	CTTAAG	1 cut(s)	421
VneI	GTGCAC	1 cut(s)	1102
VpaK11BI	GGWCC	1 cut(s)	598
XhoI	CTCGAG	2 cut(s)	435, 1276
XspI	CTAG	1 cut(s)	482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.