Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0011449

Physical Location & Seq

Genomic Coordinates Forward (+)

22493992 .. 22496713

Gene Structure 2722 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012801

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1407 bp

ATGGGCGCATTGGATGAGGGGCATTTCTGTTCGGAACTTGAGAATGGAGTGAAGCTGGGTTGTATTGAGCTGAGTGTTGATGCCGAACCCGAGACCGTAGCTATTGAGGATGCTGTCAAAGTTCTCATGCAGGGTCTTGGAGAGGATGTGAACAGAGAAGGTCTCAAGAAGACACCTCTTCGTGTTGCCAAGGCCCTTCGAGAGGGAACAAGGGGTTATAGACAAAAGGTTAAGGACATTGTGCAAGGTGCTTTGTTCCCTGAATCTGGTCTGGATAATGCAGTAGGTCATGCTGGAGGAGCTGGTGGACTAGTGGTTGTTCGAGATCTGAACCTCTTCTCCTATTGTGAATCTTGTATGCTACCATTCCAGGTTAAATGTCATGTGGGTTATGTCCCTTCTGGTCAACGAGTTGTAGGCTTAAGCAAGCTCTCTCGAGTAGCTGATGTGTTTGGAAAACGTCTTCAAGAGCCGCAGCGTCTAGCTGATGAAGTGTGTTCAGCCCTGCACCATGGCATCAAGCCAGCCGGTGTTGCTGTCATGCTCCAGTGTTCACACATTCATTTCCCAAATTTAGAATCAGCTCTTCTCGACTCGAACCACCAAGGATGGGTAAAATTGCTGGTTAGTTCAGGTTCTGGAGTTTTTGAAAATGAAAGTGCCAATATTTGGGCAGATTTTTTGAGTCTTCTGAAATTCAGAGGCATAGATGTGGAGAAAACTGGCTTGAGAGACTCAAGCAATTGGTGTCCATCTCGATCTTCAGCCGGTGCTAATGCTGTCTCCAAGGTTGAATCAGCCAATCAGGGAATGGTCACTGCAGTAGCTTCAATTCTGAGATCACTGGGTGAAGACCCATTGAGAAAGGAGCTCGTAGGAACACCTGCTCGTTTTGTGAAATGGTTGATGAACTTCCAGAATTGTAATTTGGATATGAAGCTGAATGGGTTTGTTTCTGCTAGGGTGGATCCCCTACACCCCAATGGAGATAGCTGCAAAGAAAAGCAAATCCACTCTGAGCTCAACTTGTCTGTGTGGTCTCAGTGTGAGCATCATTTACTTCCCTTTTATGGTGTCGTGCATATAGGATATGCAGCCACAGAAGCCACAGAAGGATCCAATCCCATTGGAAAATCTCTTCTACAATCAATTGTCTGTTTCTATGGTTTTAAGCTCCAGGTGCAGGAGAGACTTACACGGCAGATAGCAGAAACTGTGGCATCACTGTCAGGTGGAGATGTGATAGTTGTTGTGGAGGCTAACCACACCTGTATGATCTCTAGAGGGATTGAGAAGTTTGGAAGTAGCACAGCTACCATCGCTGTACTGGGTCGTTTTTCAACTGACCCTGCTGCAAGGGCCAAGTTTTTGCAGAGCCTCCCAAACACTGCCATTTCAGGACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003933 GO:0003934 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006725 GO:0006729 GO:0006732 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008270 GO:0008277 GO:0009058 GO:0009108 GO:0009966 GO:0009987 GO:0010646 GO:0016020 GO:0016787 GO:0016810 GO:0016814 GO:0017076 GO:0017144 GO:0018130 GO:0019001 GO:0019238 GO:0019438 GO:0019637 GO:0019751 GO:0023051 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0034311 GO:0034312 GO:0034641 GO:0035639 GO:0035998 GO:0036094 GO:0042558 GO:0042559 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046146 GO:0046165 GO:0046173 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051066 GO:0051186 GO:0051188 GO:0065007 GO:0071704 GO:0090407 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00790 ko01100 map00790 map01100

Pfam Domains

Protein Families

GTP_cyclohydroI

Protein Analysis

469

Amino Acids

50.44

Weight (kDa)

6.54

Isoelectric Point (pI)

38.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_cyclohydroI	PF01227	35 - 187	7.5e-35	GTP cyclohydrolase I
GTP_cyclohydroI	PF01227	273 - 459	5.2e-37	GTP cyclohydrolase I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011449)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07270 AT3G07270
fragaria_vesca	FvH4_2g18370 FvH4_2g18370 FvH4_2g18370
malus_domestica	MD05G1169300.v1.1 MD10G1157600.v1.1
prunus_persica	Prupe.8G196500_v2.0.a1 Prupe.8G196500_v2.0.a1
pyrus_communis	pycom05g15690
rosa_chinensis	RchiOBHm_Chr6g0283751
rosa_laevigata	RLG00000012801
rosa_multiflora	Rmu_sc0001521.1_g000012
rosa_roxburghii	Rroxscaffold_7G00185210
rosa_rugosa	Rorug06G0157600
rosa_samantha	Rh6AG269700 Rh6BG271800 Rh6CG272000 Rh6DG265600
rosa_wichuraiana	Rw6G023310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	892
Acc36I	ACCTGC	1 cut(s)	892
AccB7I	CCANNNNNTGG	1 cut(s)	669
AciI	CCGC	1 cut(s)	473
AclWI	GGATC	4 cut(s)	962, 975, 1110, 1123
AcsI	RAATTY	2 cut(s)	571, 695
AcuI	CTGAAG	1 cut(s)	747
AdeI	CACNNNGTG	1 cut(s)	848
AfaI	GTAC	1 cut(s)	1326
AfiI	CCNNNNNNNGG	6 cut(s)	202, 266, 610, 669, 1070, 1183
AflII	CTTAAG	1 cut(s)	421
AgsI	TTSAA	5 cut(s)	467, 650, 794, 831, 1341
AhlI	ACTAGT	1 cut(s)	310
AjnI	CCWGG	2 cut(s)	369, 1176
AjuI	GAANNNNNNNTTGG	2 cut(s)	911, 943
AloI	GAACNNNNNNTCC	2 cut(s)	323, 355
Alw21I	GWGCWC	2 cut(s)	873, 1023
Alw26I	GTCTC	6 cut(s)	86, 167, 726, 787, 1044, 1183
AlwI	GGATC	4 cut(s)	962, 975, 1110, 1123
AlwNI	CAGNNNCTG	2 cut(s)	638, 1214
Ama87I	CYCGRG	2 cut(s)	89, 435
AoxI	GGCC	2 cut(s)	192, 1359
ApeKI	GCWGC	4 cut(s)	475, 993, 1094, 1352
ApoI	RAATTY	2 cut(s)	571, 695
Asp700I	GAANNNNTTC	1 cut(s)	335
AspLEI	GCGC	1 cut(s)	8
AspS9I	GGNCC	3 cut(s)	193, 1359, 1400
AsuHPI	GGTGA	1 cut(s)	860
AvaI	CYCGRG	2 cut(s)	89, 435
AvaII	GGWCC	1 cut(s)	1400
BamHI	GGATCC	2 cut(s)	967, 1115
BanII	GRGCYC	2 cut(s)	873, 1023
BbsI	GAAGAC	4 cut(s)	176, 455, 680, 858
Bbv12I	GWGCWC	2 cut(s)	873, 1023
BbvI	GCAGC	4 cut(s)	487, 980, 1106, 1339
BccI	CCATC	3 cut(s)	603, 760, 1325
BceAI	ACGGC	1 cut(s)	1214
BciT130I	CCWGG	2 cut(s)	371, 1178
BcoDI	GTCTC	6 cut(s)	86, 167, 726, 787, 1044, 1183
BcuI	ACTAGT	1 cut(s)	310
BfaI	CTAG	4 cut(s)	311, 482, 960, 1281
BfmI	CTRYAG	1 cut(s)	819
BfrI	CTTAAG	1 cut(s)	421
BfuAI	ACCTGC	1 cut(s)	892
BglII	AGATCT	1 cut(s)	325
BisI	GCNGC	5 cut(s)	473, 476, 994, 1095, 1353
BlsI	GCNGC	5 cut(s)	474, 477, 995, 1096, 1354
Bme1390I	CCNGG	2 cut(s)	371, 1178
Bme18I	GGWCC	1 cut(s)	1400
BmeT110I	CYCGRG	2 cut(s)	89, 435
BmgT120I	GGNCC	3 cut(s)	193, 1359, 1400
BmiI	GGNNCC	2 cut(s)	969, 1117
BmrFI	CCNGG	2 cut(s)	371, 1178
BmrI	ACTGGG	2 cut(s)	854, 1337
BmsI	GCATC	5 cut(s)	70, 100, 525, 1060, 1229
BmuI	ACTGGG	2 cut(s)	854, 1337
BpiI	GAAGAC	4 cut(s)	176, 455, 680, 858
BplI	GAGNNNNNCTC	2 cut(s)	147, 179
BpmI	CTGGAG	4 cut(s)	315, 530, 660, 1160
BpuEI	CTTGAG	4 cut(s)	59, 149, 721, 748
BsaI	GGTCTC	3 cut(s)	86, 167, 1044
BsaJI	CCNNGG	4 cut(s)	189, 511, 604, 786
BsaXI	ACNNNNNCTCC	4 cut(s)	132, 162, 323, 353
Bsc4I	CCNNNNNNNGG	6 cut(s)	202, 266, 610, 669, 1070, 1183
Bse118I	RCCGGY	2 cut(s)	527, 767
Bse1I	ACTGG	4 cut(s)	547, 727, 849, 1332
BseBI	CCWGG	2 cut(s)	371, 1178
BseDI	CCNNGG	4 cut(s)	189, 511, 604, 786
BseGI	GGATG	4 cut(s)	19, 115, 151, 614
BseLI	CCNNNNNNNGG	6 cut(s)	202, 266, 610, 669, 1070, 1183
BseMII	CTCAG	4 cut(s)	62, 827, 1008, 1055
BseNI	ACTGG	4 cut(s)	547, 727, 849, 1332
BseRI	GAGGAG	1 cut(s)	312
BseXI	GCAGC	4 cut(s)	487, 980, 1106, 1339
BseYI	CCCAGC	1 cut(s)	55
BsgI	GTGCAG	2 cut(s)	491, 1202
BshFI	GGCC	2 cut(s)	194, 1361
BsiHKAI	GWGCWC	2 cut(s)	873, 1023
BsiHKCI	CYCGRG	2 cut(s)	89, 435
BsiSI	CCGG	2 cut(s)	528, 768
BslFI	GGGAC	1 cut(s)	380
BslI	CCNNNNNNNGG	6 cut(s)	202, 266, 610, 669, 1070, 1183
BsmAI	GTCTC	6 cut(s)	86, 167, 726, 787, 1044, 1183
BsmFI	GGGAC	1 cut(s)	380
BsnI	GGCC	2 cut(s)	194, 1361
Bso31I	GGTCTC	3 cut(s)	86, 167, 1044
BsoBI	CYCGRG	2 cut(s)	89, 435
Bsp1286I	GDGCHC	2 cut(s)	873, 1023
Bsp143I	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
Bsp19I	CCATGG	1 cut(s)	511
BspACI	CCGC	1 cut(s)	473
BspANI	GGCC	2 cut(s)	194, 1361
BspCNI	CTCAG	4 cut(s)	63, 828, 1009, 1054
BspLI	GGNNCC	2 cut(s)	969, 1117
BspMAI	CTGCAG	1 cut(s)	823
BspMI	ACCTGC	1 cut(s)	892
BspPI	GGATC	4 cut(s)	962, 975, 1110, 1123
BspQI	GCTCTTC	1 cut(s)	591
BspTI	CTTAAG	1 cut(s)	421
BspTNI	GGTCTC	3 cut(s)	86, 167, 1044
BsrFI	RCCGGY	2 cut(s)	527, 767
BsrI	ACTGG	4 cut(s)	547, 727, 849, 1332
BssAI	RCCGGY	2 cut(s)	527, 767
BssECI	CCNNGG	4 cut(s)	189, 511, 604, 786
BssMI	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
BssT1I	CCWWGG	4 cut(s)	189, 511, 604, 786
Bst2UI	CCWGG	2 cut(s)	371, 1178
Bst4CI	ACNGT	4 cut(s)	97, 1216, 1227, 1404
Bst6I	CTCTTC	4 cut(s)	183, 341, 591, 1143
BstAFI	CTTAAG	1 cut(s)	421
BstC8I	GCNNGC	2 cut(s)	428, 525
BstDEI	CTNAG	4 cut(s)	71, 836, 1017, 1041
BstDSI	CCRYGG	1 cut(s)	511
BstENI	CCTNNNNNAGG	1 cut(s)	200
BstF5I	GGATG	4 cut(s)	19, 115, 151, 614
BstHHI	GCGC	1 cut(s)	8
BstKTI	GATC	6 cut(s)	328, 761, 842, 970, 1118, 1278
BstMAI	GTCTC	6 cut(s)	86, 167, 726, 787, 1044, 1183
BstMBI	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
BstMWI	GCNNNNNNNGC	6 cut(s)	299, 533, 1103, 1180, 1319, 1358
BstNI	CCWGG	2 cut(s)	371, 1178
BstSCI	CCNGG	2 cut(s)	369, 1176
BstSFI	CTRYAG	1 cut(s)	819
BstV1I	GCAGC	4 cut(s)	487, 980, 1106, 1339
BstV2I	GAAGAC	4 cut(s)	176, 455, 680, 858
BstX2I	RGATCY	3 cut(s)	325, 967, 1115
BstYI	RGATCY	3 cut(s)	325, 967, 1115
BsuRI	GGCC	2 cut(s)	194, 1361
BtgI	CCRYGG	1 cut(s)	511
BtgZI	GCGATG	1 cut(s)	1303
BtsCI	GGATG	4 cut(s)	19, 115, 151, 614
BtsI	GCAGTG	2 cut(s)	816, 1386
BtsIMutI	CAGTG	6 cut(s)	554, 816, 842, 1049, 1223, 1386
BveI	ACCTGC	1 cut(s)	892
Cac8I	GCNNGC	2 cut(s)	428, 525
CaiI	CAGNNNCTG	2 cut(s)	638, 1214
CfoI	GCGC	1 cut(s)	8
Cfr10I	RCCGGY	2 cut(s)	527, 767
Cfr13I	GGNCC	3 cut(s)	193, 1359, 1400
CseI	GACGC	1 cut(s)	467
Csp6I	GTAC	1 cut(s)	1325
CviAII	CATG	5 cut(s)	127, 290, 383, 512, 541
CviQI	GTAC	1 cut(s)	1325
DdeI	CTNAG	4 cut(s)	71, 836, 1017, 1041
DpnI	GATC	6 cut(s)	327, 760, 841, 969, 1117, 1277
DpnII	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
DraIII	CACNNNGTG	1 cut(s)	848
Eam1104I	CTCTTC	4 cut(s)	183, 341, 591, 1143
EarI	CTCTTC	4 cut(s)	183, 341, 591, 1143
Ecl136II	GAGCTC	2 cut(s)	871, 1021
Eco130I	CCWWGG	4 cut(s)	189, 511, 604, 786
Eco24I	GRGCYC	2 cut(s)	873, 1023
Eco31I	GGTCTC	3 cut(s)	86, 167, 1044
Eco47I	GGWCC	1 cut(s)	1400
Eco53kI	GAGCTC	2 cut(s)	871, 1021
Eco57I	CTGAAG	1 cut(s)	747
Eco88I	CYCGRG	2 cut(s)	89, 435
EcoICRI	GAGCTC	2 cut(s)	871, 1021
EcoNI	CCTNNNNNAGG	1 cut(s)	200
EcoO109I	RGGNCCY	1 cut(s)	193
EcoRII	CCWGG	2 cut(s)	369, 1176
EcoT14I	CCWWGG	4 cut(s)	189, 511, 604, 786
EcoT38I	GRGCYC	2 cut(s)	873, 1023
ErhI	CCWWGG	4 cut(s)	189, 511, 604, 786
FaeI	CATG	5 cut(s)	130, 293, 386, 515, 544
FaqI	GGGAC	1 cut(s)	380
FatI	CATG	5 cut(s)	126, 289, 382, 511, 540
Fnu4HI	GCNGC	5 cut(s)	473, 476, 994, 1095, 1353
FokI	GGATG	4 cut(s)	26, 122, 158, 621
FriOI	GRGCYC	2 cut(s)	873, 1023
Fsp4HI	GCNGC	5 cut(s)	473, 476, 994, 1095, 1353
FspBI	CTAG	4 cut(s)	311, 482, 960, 1281
GlaI	GCGC	1 cut(s)	7
GluI	GCNGC	5 cut(s)	473, 476, 994, 1095, 1353
GsaI	CCCAGC	1 cut(s)	59
GsuI	CTGGAG	4 cut(s)	315, 530, 660, 1160
HaeIII	GGCC	2 cut(s)	194, 1361
HapII	CCGG	2 cut(s)	528, 768
HgaI	GACGC	1 cut(s)	467
HhaI	GCGC	1 cut(s)	8
Hin1II	CATG	5 cut(s)	130, 293, 386, 515, 544
Hin6I	GCGC	1 cut(s)	6
HinP1I	GCGC	1 cut(s)	6
HincII	GTYRAC	1 cut(s)	407
HindII	GTYRAC	1 cut(s)	407
HinfI	GANTC	7 cut(s)	263, 350, 578, 593, 685, 734, 794
HpaII	CCGG	2 cut(s)	528, 768
HphI	GGTGA	1 cut(s)	860
Hpy166II	GTNNAC	4 cut(s)	151, 308, 407, 554
Hpy188I	TCNGA	6 cut(s)	34, 330, 693, 701, 837, 1018
Hpy8I	GTNNAC	4 cut(s)	151, 308, 407, 554
HpyAV	CCTTC	4 cut(s)	152, 206, 408, 1106
HpyCH4III	ACNGT	4 cut(s)	97, 1216, 1227, 1404
HpyCH4IV	ACGT	1 cut(s)	460
HpyF10VI	GCNNNNNNNGC	6 cut(s)	299, 533, 1103, 1180, 1319, 1358
HpyF3I	CTNAG	4 cut(s)	71, 836, 1017, 1041
HpySE526I	ACGT	1 cut(s)	460
Hsp92II	CATG	5 cut(s)	130, 293, 386, 515, 544
HspAI	GCGC	1 cut(s)	6
Kzo9I	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
LguI	GCTCTTC	1 cut(s)	591
LmnI	GCTCC	4 cut(s)	299, 549, 868, 1179
Lsp1109I	GCAGC	4 cut(s)	487, 980, 1106, 1339
LweI	GCATC	5 cut(s)	70, 100, 525, 1060, 1229
MaeI	CTAG	4 cut(s)	311, 482, 960, 1281
MaeII	ACGT	1 cut(s)	460
MaeIII	GTNAC	1 cut(s)	814
MalI	GATC	6 cut(s)	327, 760, 841, 969, 1117, 1277
MboI	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
MboII	GAAGA	9 cut(s)	170, 181, 328, 455, 578, 680, 753, 863, 1130
MfeI	CAATTG	2 cut(s)	742, 1149
MflI	RGATCY	3 cut(s)	325, 967, 1115
MhlI	GDGCHC	2 cut(s)	873, 1023
MluCI	AATT	8 cut(s)	571, 617, 695, 742, 831, 919, 925, 1149
MlyI	GAGTC	3 cut(s)	587, 694, 728
MroXI	GAANNNNTTC	1 cut(s)	335
MseI	TTAA	4 cut(s)	231, 375, 422, 1170
MslI	CAYNNNNRTG	3 cut(s)	710, 981, 1271
MspCI	CTTAAG	1 cut(s)	421
MspI	CCGG	2 cut(s)	528, 768
MspR9I	CCNGG	2 cut(s)	371, 1178
MunI	CAATTG	2 cut(s)	742, 1149
MvaI	CCWGG	2 cut(s)	371, 1178
MwoI	GCNNNNNNNGC	6 cut(s)	299, 533, 1103, 1180, 1319, 1358
NcoI	CCATGG	1 cut(s)	511
NdeII	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
NlaIII	CATG	5 cut(s)	130, 293, 386, 515, 544
NlaIV	GGNNCC	2 cut(s)	969, 1117
NmuCI	GTSAC	1 cut(s)	814
PaeR7I	CTCGAG	1 cut(s)	435
PaqCI	CACCTGC	1 cut(s)	892
PciSI	GCTCTTC	1 cut(s)	591
PdmI	GAANNNNTTC	1 cut(s)	335
PfeI	GAWTC	4 cut(s)	263, 350, 578, 794
PflMI	CCANNNNNTGG	1 cut(s)	669
PkrI	GCNGC	5 cut(s)	474, 477, 995, 1096, 1354
PleI	GAGTC	3 cut(s)	587, 693, 728
PpsI	GAGTC	3 cut(s)	587, 693, 728
Psp124BI	GAGCTC	2 cut(s)	873, 1023
Psp6I	CCWGG	2 cut(s)	369, 1176
PspFI	CCCAGC	1 cut(s)	55
PspGI	CCWGG	2 cut(s)	369, 1176
PspN4I	GGNNCC	2 cut(s)	969, 1117
PspPI	GGNCC	3 cut(s)	193, 1359, 1400
PstI	CTGCAG	1 cut(s)	823
PstNI	CAGNNNCTG	2 cut(s)	638, 1214
PsuI	RGATCY	3 cut(s)	325, 967, 1115
RsaI	GTAC	1 cut(s)	1326
RsaNI	GTAC	1 cut(s)	1325
RseI	CAYNNNNRTG	3 cut(s)	710, 981, 1271
SacI	GAGCTC	2 cut(s)	873, 1023
SapI	GCTCTTC	1 cut(s)	591
SaqAI	TTAA	4 cut(s)	231, 375, 422, 1170
SatI	GCNGC	5 cut(s)	473, 476, 994, 1095, 1353
Sau3AI	GATC	6 cut(s)	325, 758, 839, 967, 1115, 1275
Sau96I	GGNCC	3 cut(s)	193, 1359, 1400
SchI	GAGTC	3 cut(s)	587, 694, 728
ScrFI	CCNGG	2 cut(s)	371, 1178
SduI	GDGCHC	2 cut(s)	873, 1023
SfaNI	GCATC	5 cut(s)	70, 100, 525, 1060, 1229
SfcI	CTRYAG	1 cut(s)	819
Sfr274I	CTCGAG	1 cut(s)	435
SinI	GGWCC	1 cut(s)	1400
SlaI	CTCGAG	1 cut(s)	435
SmiMI	CAYNNNNRTG	3 cut(s)	710, 981, 1271
SmlI	CTYRAG	6 cut(s)	38, 164, 421, 435, 727, 736
SmoI	CTYRAG	6 cut(s)	38, 164, 421, 435, 727, 736
SpeI	ACTAGT	1 cut(s)	310
Sse9I	AATT	8 cut(s)	571, 617, 695, 742, 831, 919, 925, 1149
SsiI	CCGC	1 cut(s)	473
SspI	AATATT	1 cut(s)	667
SspMI	CTAG	4 cut(s)	311, 482, 960, 1281
SstI	GAGCTC	2 cut(s)	873, 1023
StyD4I	CCNGG	2 cut(s)	369, 1176
StyI	CCWWGG	4 cut(s)	189, 511, 604, 786
TaaI	ACNGT	4 cut(s)	97, 1216, 1227, 1404
TaiI	ACGT	1 cut(s)	463
TaqI	TCGA	6 cut(s)	199, 322, 436, 591, 596, 757
TasI	AATT	8 cut(s)	571, 617, 695, 742, 831, 919, 925, 1149
TatI	WGTACW	1 cut(s)	1324
TauI	GCSGC	1 cut(s)	475
TfiI	GAWTC	4 cut(s)	263, 350, 578, 794
Tru1I	TTAA	4 cut(s)	231, 375, 422, 1170
Tru9I	TTAA	4 cut(s)	231, 375, 422, 1170
TscAI	CASTG	6 cut(s)	554, 823, 849, 1049, 1230, 1393
TseFI	GTSAC	1 cut(s)	814
TseI	GCWGC	4 cut(s)	475, 993, 1094, 1352
Tsp45I	GTSAC	1 cut(s)	814
TspDTI	ATGAA	5 cut(s)	504, 551, 669, 923, 950
TspRI	CASTG	6 cut(s)	554, 823, 849, 1049, 1230, 1393
Van91I	CCANNNNNTGG	1 cut(s)	669
Vha464I	CTTAAG	1 cut(s)	421
VpaK11BI	GGWCC	1 cut(s)	1400
XagI	CCTNNNNNAGG	1 cut(s)	200
XapI	RAATTY	2 cut(s)	571, 695
XbaI	TCTAGA	1 cut(s)	1280
XcmI	CCANNNNNNNNNTGG	2 cut(s)	808, 1324
XhoI	CTCGAG	1 cut(s)	435
XmnI	GAANNNNTTC	1 cut(s)	335
XspI	CTAG	4 cut(s)	311, 482, 960, 1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.