Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0005698

Physical Location & Seq

Genomic Coordinates Forward (+)

3329835 .. 3336614

Gene Structure 6780 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g04460.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGATGTGGACAACGTGCCACTGGACGACAAGGCGAAGAGGATGAGGGATCTGCTGTCGAGCTTCTACTCCCCTGACCCTTCGCTGTCGTCTCCCAACTCTAACTCCTCGTCCAAGTACGCCACTCTCGACGCCATCAACTCCACCTCCTTCGATCCCGACCAGTACATGCATCTCCTCGTACACAAGTCAAACTTGGAAGGGCTTCTACAGAGACATGTTCAAATGGCTGCTGAGATAAAGAATCTTGATACTGACTTACAAATGTTAGTATACGAGAACTACAACAAGTTTATTAGCGCGACAGATACTATTAAACAGATGAAAAGTAATATTGTGGGGATGGAGGCAAATATGGAACAGCTCCTTGAGAAGATAATGTCTGTGCAATCTAGAAGTGATGGGGTCAACACTTCTCTTTCTGAGAAGAGGGAACACATAGAAAAGTTGCATCGCACACGCAACCTTCTTCGTAAAGTTCAGTTCATATATGATCTACCTGCTAGGCTTGGGAAGTGTATCAAATCAGAAGCATATGCTGATGCAGTCAAGTTTTATACAGGAGCAATACCAATATTTAAGGCATATGGAGACTCCTCATTCCAGGATTGTAAGCGAGCATCTGAAGAAGCTGTGACCATAATTATCAAGAATTTGCAGGGAAAGCTGTTCTCAGATTCTGAATCCATACAAGCAAGAGCTGAGGCTGCTGTGCTTCTTAAGCAGTTGGATTTCCCAGTTGACAGCTTAAAGGTGAAGTTGCTTGAGAAGTTGGAACAATCTGTTGCTGGCCTTCAGCTTAAGATAGAAGATATAGGCAATTCTTCACTGGATTCTAATGATCCTTCTACTGACACAGTTCCTGCTACTGCACATGAGACTTCTGTGCATGAGTTTGCGGAGGCTGTCCGTGCTTATCGCGTAATATTTCCTGATTCGGATACACAACTAACCAAGCTTGCACAAGACTTGGTTGCCGGGCACTTCAAAACCACTGAGCAATGCATAAAGACAGAAATCTGGTCAGCAGATCTACTAGGAGTTCTTCGGATTATATGGAGAGATGTACTTCTGATGGATGATGTGTTAAATGAGGATGCTCTCCTTGATTATTCTCTGGAGGCTGCTCGGGTTACTGTGAAACTATATGTTAGCAACAAATTTTCTCATCTCCTAAACAACAGTTCAGATGCCCTCACAAAAGCCCACACTAGACAGAAGGACAGAGAAGAGTATTCCTTACAAGTTGCTCTGGAGGGCGGAAAAAAGGCAGTGCTCCAAGGCAGCATGGATGTCTTACTGGACTTCCGCCAACTTCTAGATGATAATTTGGAACTGCTAGTTAAACTGAAAGACTTGATTATTGATTGGGTCCAAGAAGGGTTTCAAGACTTCTTCAGGGCACTTGAAGGTCACTTTCTCTTGCTTTCAGGAAAGAATAGTTCAGCCAGTCAAGATCAAGGTTTGACAGAGGGAATACAAGATGACAAAGTTCTGGCTGGGCTTGTCCTCGTGCTGGCCCAAGTTTCTGTTTTCATTGAACAAAATGCCATCCCAAGAATCACCGAGGAAATAGCTGCTTCCTTTTCTGGTGGTGGTGTTAGAAGCTATGAATATGGACCCACTTTCATTCCTGGAGAAATCTGTCGGATTTTCCGCTTAGCGGGTGAAAAGTTTCTGCACATTTACATAAATATGAGAACTCAGCGGATATCAGTTCTCTTAAAGAAGAGGTTTACAACACCAAATTGGGTCCAGCACAAGGAACCCCGCGAAGTTCATATGTTTGTTGATTTATTTCTTCAAGAGTTGGAAGCAATTAGAAGTGAAGTGAAGCAGATTTTGCCCCAAGGTGTTCGCAGACATCGCCGGGCTGACAGCAACGGAAGCACTGCCTCATCTCGGAGCAACCCATTACGAGAGGAAAAATTAAGTCGGTCAAACACCCAGAGGGCTAGAAGCCAGCTGTTGGAAACCCATCTAGCAAAACTTTTTAAGCAAAAAGTTGAAATATTTACGAAAGTTGATTTTACGCAGGAGTCGGTGGTAACGACCTTGGTAAAACTTTGTCTTAAAAGCTTGCAAGAATTTGTCAGACTCCAAACTTTTAATAGGAGTGGATTCCAGCAAATTCAGTTGGATATACAGTTCCTAAGGACTCCTTTAAAGGAAATGGCAGAAGATGAAGCTGCTGTTGACTTTCTGCTGGATGAGTGGCGTGATTGTTGCAGCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000938 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0006810 GO:0006914 GO:0007034 GO:0007041 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0010051 GO:0010305 GO:0012505 GO:0016192 GO:0016197 GO:0016482 GO:0031410 GO:0031982 GO:0032456 GO:0032501 GO:0032502 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055037 GO:0061919 GO:0097708 GO:0099023 GO:1990745

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps51

Protein Analysis

746

Amino Acids

84.43

Weight (kDa)

5.78

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02030 AT4G02030
fragaria_vesca	FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210 FvH4_5g39210
malus_domestica	MD03G1295300.v1.1
prunus_persica	Prupe.5G007800_v2.0.a1 Prupe.5G007800_v2.0.a1
pyrus_communis	pycom01g04460
rosa_chinensis	RchiOBHm_Chr7g0241471
rosa_laevigata	RLG00000000578
rosa_multiflora	Rmu_sc0040921.1_g000003
rosa_roxburghii	Rroxscaffold_3G00219100
rosa_rugosa	Rorug07G0344000 Rorug07G0344100 Rorug07G0344200
rosa_samantha	Rh7AG498800 Rh7BG469400 Rh7CG515700 Rh7DG483500
rosa_wichuraiana	Rw7G042130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	508
AccB7I	CCANNNNNTGG	1 cut(s)	1969
AccI	GTMKAC	1 cut(s)	273
AccII	CGCG	3 cut(s)	302, 921, 1773
AciI	CCGC	7 cut(s)	899, 1260, 1309, 1657, 1664, 1708, 1771
AclWI	GGATC	3 cut(s)	57, 149, 836
AcsI	RAATTY	4 cut(s)	652, 1160, 2087, 2130
AcuI	CTGAAG	3 cut(s)	645, 779, 1381
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	4 cut(s)	119, 167, 183, 1068
AfiI	CCNNNNNNNGG	5 cut(s)	1516, 1663, 1762, 1902, 1969
AflII	CTTAAG	2 cut(s)	719, 800
AflIII	ACRYGT	1 cut(s)	217
AgsI	TTSAA	7 cut(s)	224, 988, 1388, 1409, 1541, 1805, 2009
AjnI	CCWGG	2 cut(s)	603, 1633
AjuI	GAANNNNNNNTTGG	2 cut(s)	1368, 1400
AloI	GAACNNNNNNTCC	2 cut(s)	2132, 2164
Alw21I	GWGCWC	1 cut(s)	1278
Alw26I	GTCTC	4 cut(s)	96, 208, 585, 872
AlwI	GGATC	3 cut(s)	57, 149, 836
AlwNI	CAGNNNCTG	3 cut(s)	680, 863, 2235
Ama87I	CYCGRG	1 cut(s)	1128
AoxI	GGCC	2 cut(s)	790, 1518
ApeKI	GCWGC	8 cut(s)	230, 707, 1124, 1284, 1577, 2189, 2229, 2232
ApoI	RAATTY	4 cut(s)	652, 1160, 2087, 2130
Asp700I	GAANNNNTTC	3 cut(s)	204, 1383, 1674
AspLEI	GCGC	1 cut(s)	302
AspS9I	GGNCC	4 cut(s)	1372, 1519, 1619, 1753
AsuC2I	CCSGG	2 cut(s)	979, 1871
AsuHPI	GGTGA	3 cut(s)	766, 1555, 1679
AvaI	CYCGRG	1 cut(s)	1128
AvaII	GGWCC	3 cut(s)	1372, 1619, 1753
AxyI	CCTNAGG	1 cut(s)	2153
BaeGI	GKGCMC	2 cut(s)	984, 1405
BarI	GAAGNNNNNNTAC	2 cut(s)	192, 224
BauI	CACGAG	1 cut(s)	1511
Bbv12I	GWGCWC	1 cut(s)	1278
BbvCI	CCTCAGC	1 cut(s)	702
BbvI	GCAGC	6 cut(s)	217, 694, 1111, 1296, 1564, 2176
BccI	CCATC	6 cut(s)	143, 337, 395, 1069, 1559, 1986
BciT130I	CCWGG	2 cut(s)	605, 1635
BciVI	GTATCC	1 cut(s)	934
BcnI	CCSGG	2 cut(s)	979, 1871
BcoDI	GTCTC	4 cut(s)	96, 208, 585, 872
BfaI	CTAG	8 cut(s)	393, 504, 1037, 1212, 1319, 1340, 1956, 1982
BfmI	CTRYAG	1 cut(s)	209
BfrI	CTTAAG	2 cut(s)	719, 800
BfuAI	ACCTGC	1 cut(s)	508
BfuI	GTATCC	1 cut(s)	934
BglII	AGATCT	1 cut(s)	1030
BisI	GCNGC	8 cut(s)	231, 708, 1125, 1285, 1578, 2190, 2230, 2233
BlpI	GCTNAGC	1 cut(s)	1660
BlsI	GCNGC	8 cut(s)	232, 709, 1126, 1286, 1579, 2191, 2231, 2234
Bme1390I	CCNGG	4 cut(s)	605, 979, 1635, 1871
Bme18I	GGWCC	3 cut(s)	1372, 1619, 1753
BmeT110I	CYCGRG	1 cut(s)	1128
BmgT120I	GGNCC	4 cut(s)	1372, 1519, 1619, 1753
BmiI	GGNNCC	4 cut(s)	1373, 1621, 1754, 1767
BmrFI	CCNGG	4 cut(s)	605, 979, 1635, 1871
BmrI	ACTGGG	1 cut(s)	731
BmsI	GCATC	6 cut(s)	181, 460, 532, 629, 1087, 1180
BmuI	ACTGGG	1 cut(s)	731
BpmI	CTGGAG	3 cut(s)	1139, 1274, 1656
Bpu10I	CCTNAGC	1 cut(s)	702
Bpu1102I	GCTNAGC	1 cut(s)	1660
BpuEI	CTTGAG	2 cut(s)	389, 785
BpuMI	CCSGG	2 cut(s)	979, 1871
BsaHI	GRCGYC	1 cut(s)	132
BsaJI	CCNNGG	4 cut(s)	1279, 1566, 1849, 2055
BsaXI	ACNNNNNCTCC	4 cut(s)	89, 119, 131, 161
Bsc4I	CCNNNNNNNGG	5 cut(s)	1516, 1663, 1762, 1902, 1969
Bse1I	ACTGG	6 cut(s)	27, 163, 737, 834, 1305, 1449
Bse21I	CCTNAGG	1 cut(s)	2153
Bse3DI	GCAATG	1 cut(s)	1007
BseBI	CCWGG	2 cut(s)	605, 1635
BseDI	CCNNGG	4 cut(s)	1279, 1566, 1849, 2055
BseGI	GGATG	8 cut(s)	10, 48, 348, 1084, 1102, 1297, 1551, 2215
BseLI	CCNNNNNNNGG	5 cut(s)	1516, 1663, 1762, 1902, 1969
BseMI	GCAATG	1 cut(s)	1007
BseMII	CTCAG	6 cut(s)	225, 414, 687, 693, 987, 1718
BseNI	ACTGG	6 cut(s)	27, 163, 737, 834, 1305, 1449
BseRI	GAGGAG	3 cut(s)	97, 167, 586
BseSI	GKGCMC	2 cut(s)	984, 1405
BseXI	GCAGC	6 cut(s)	217, 694, 1111, 1296, 1564, 2176
BseYI	CCCAGC	1 cut(s)	1499
BsgI	GTGCAG	2 cut(s)	855, 1664
Bsh1236I	CGCG	3 cut(s)	302, 921, 1773
BshFI	GGCC	2 cut(s)	792, 1520
BsiHKAI	GWGCWC	1 cut(s)	1278
BsiHKCI	CYCGRG	1 cut(s)	1128
BsiSI	CCGG	2 cut(s)	978, 1870
BslI	CCNNNNNNNGG	5 cut(s)	1516, 1663, 1762, 1902, 1969
BsmAI	GTCTC	4 cut(s)	96, 208, 585, 872
BsmBI	CGTCTC	1 cut(s)	96
BsnI	GGCC	2 cut(s)	792, 1520
BsoBI	CYCGRG	1 cut(s)	1128
Bsp1286I	GDGCHC	3 cut(s)	984, 1278, 1405
Bsp143I	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
Bsp1720I	GCTNAGC	1 cut(s)	1660
BspACI	CCGC	7 cut(s)	899, 1260, 1309, 1657, 1664, 1708, 1771
BspANI	GGCC	2 cut(s)	792, 1520
BspCNI	CTCAG	6 cut(s)	226, 415, 686, 694, 988, 1717
BspFNI	CGCG	3 cut(s)	302, 921, 1773
BspLI	GGNNCC	4 cut(s)	1373, 1621, 1754, 1767
BspMI	ACCTGC	1 cut(s)	508
BspPI	GGATC	3 cut(s)	57, 149, 836
BspTI	CTTAAG	2 cut(s)	719, 800
BsrDI	GCAATG	1 cut(s)	1007
BsrI	ACTGG	6 cut(s)	27, 163, 737, 834, 1305, 1449
BssECI	CCNNGG	4 cut(s)	1279, 1566, 1849, 2055
BssMI	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
BssNAI	GTATAC	1 cut(s)	274
BssNI	GRCGYC	1 cut(s)	132
BssSI	CACGAG	1 cut(s)	1511
BssT1I	CCWWGG	3 cut(s)	1279, 1849, 2055
Bst1107I	GTATAC	1 cut(s)	274
Bst2BI	CACGAG	1 cut(s)	1511
Bst2UI	CCWGG	2 cut(s)	605, 1635
Bst4CI	ACNGT	4 cut(s)	859, 1138, 1184, 2148
Bst6I	CTCTTC	4 cut(s)	32, 422, 1224, 1724
BstACI	GRCGYC	1 cut(s)	132
BstAFI	CTTAAG	2 cut(s)	719, 800
BstAPI	GCANNNNNTGC	1 cut(s)	1843
BstC8I	GCNNGC	6 cut(s)	618, 790, 960, 1518, 1964, 2081
BstDEI	CTNAG	8 cut(s)	234, 423, 673, 702, 996, 1660, 1704, 2153
BstF5I	GGATG	8 cut(s)	10, 48, 348, 1084, 1102, 1297, 1551, 2215
BstFNI	CGCG	3 cut(s)	302, 921, 1773
BstHHI	GCGC	1 cut(s)	302
BstKTI	GATC	6 cut(s)	52, 157, 496, 844, 1033, 1459
BstMAI	GTCTC	4 cut(s)	96, 208, 585, 872
BstMBI	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
BstMWI	GCNNNNNNNGC	7 cut(s)	664, 707, 721, 911, 1843, 1866, 1887
BstNI	CCWGG	2 cut(s)	605, 1635
BstNSI	RCATGY	2 cut(s)	172, 221
BstSCI	CCNGG	4 cut(s)	603, 977, 1633, 1869
BstSFI	CTRYAG	1 cut(s)	209
BstSLI	GKGCMC	2 cut(s)	984, 1405
BstUI	CGCG	3 cut(s)	302, 921, 1773
BstV1I	GCAGC	6 cut(s)	217, 694, 1111, 1296, 1564, 2176
BstX2I	RGATCY	2 cut(s)	49, 1030
BstYI	RGATCY	2 cut(s)	49, 1030
BstZ17I	GTATAC	1 cut(s)	274
Bsu36I	CCTNAGG	1 cut(s)	2153
BsuI	GTATCC	1 cut(s)	934
BsuRI	GGCC	2 cut(s)	792, 1520
BtgZI	GCGATG	2 cut(s)	437, 1850
BtsCI	GGATG	8 cut(s)	10, 48, 348, 1084, 1102, 1297, 1551, 2215
BtsI	GCAGTG	2 cut(s)	1278, 1890
BtsIMutI	CAGTG	5 cut(s)	20, 827, 993, 1278, 1890
BveI	ACCTGC	1 cut(s)	508
Cac8I	GCNNGC	6 cut(s)	618, 790, 960, 1518, 1964, 2081
CaiI	CAGNNNCTG	3 cut(s)	680, 863, 2235
CfoI	GCGC	1 cut(s)	302
Cfr13I	GGNCC	4 cut(s)	1372, 1519, 1619, 1753
CseI	GACGC	1 cut(s)	140
Csp6I	GTAC	4 cut(s)	118, 166, 182, 1067
CviAII	CATG	5 cut(s)	169, 218, 875, 890, 1288
CviQI	GTAC	4 cut(s)	118, 166, 182, 1067
DdeI	CTNAG	8 cut(s)	234, 423, 673, 702, 996, 1660, 1704, 2153
DpnI	GATC	6 cut(s)	51, 156, 495, 843, 1032, 1458
DpnII	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
DraI	TTTAAA	1 cut(s)	2166
Eam1104I	CTCTTC	4 cut(s)	32, 422, 1224, 1724
EarI	CTCTTC	4 cut(s)	32, 422, 1224, 1724
EciI	GGCGGA	2 cut(s)	1275, 1298
Eco130I	CCWWGG	3 cut(s)	1279, 1849, 2055
Eco32I	GATATC	1 cut(s)	1713
Eco47I	GGWCC	3 cut(s)	1372, 1619, 1753
Eco57I	CTGAAG	3 cut(s)	645, 779, 1381
Eco81I	CCTNAGG	1 cut(s)	2153
Eco88I	CYCGRG	1 cut(s)	1128
EcoRII	CCWGG	2 cut(s)	603, 1633
EcoRV	GATATC	1 cut(s)	1713
EcoT14I	CCWWGG	3 cut(s)	1279, 1849, 2055
EcoT22I	ATGCAT	2 cut(s)	174, 1007
ErhI	CCWWGG	3 cut(s)	1279, 1849, 2055
Esp3I	CGTCTC	1 cut(s)	96
FaeI	CATG	5 cut(s)	172, 221, 878, 893, 1291
FalI	AAGNNNNNCTT	4 cut(s)	179, 211, 2146, 2178
FatI	CATG	5 cut(s)	168, 217, 874, 889, 1287
FauI	CCCGC	2 cut(s)	1657, 1778
FauNDI	CATATG	3 cut(s)	535, 586, 1782
FblI	GTMKAC	1 cut(s)	273
Fnu4HI	GCNGC	8 cut(s)	231, 708, 1125, 1285, 1578, 2190, 2230, 2233
FokI	GGATG	8 cut(s)	17, 55, 355, 1091, 1109, 1304, 1538, 2222
Fsp4HI	GCNGC	8 cut(s)	231, 708, 1125, 1285, 1578, 2190, 2230, 2233
FspBI	CTAG	8 cut(s)	393, 504, 1037, 1212, 1319, 1340, 1956, 1982
GlaI	GCGC	1 cut(s)	301
GluI	GCNGC	8 cut(s)	231, 708, 1125, 1285, 1578, 2190, 2230, 2233
GsaI	CCCAGC	1 cut(s)	1503
GsuI	CTGGAG	3 cut(s)	1139, 1274, 1656
HaeIII	GGCC	2 cut(s)	792, 1520
HapII	CCGG	2 cut(s)	978, 1870
HgaI	GACGC	1 cut(s)	140
HhaI	GCGC	1 cut(s)	302
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	5 cut(s)	172, 221, 878, 893, 1291
Hin6I	GCGC	1 cut(s)	300
HinP1I	GCGC	1 cut(s)	300
HincII	GTYRAC	3 cut(s)	409, 742, 2197
HindII	GTYRAC	3 cut(s)	409, 742, 2197
HindIII	AAGCTT	2 cut(s)	956, 2077
HpaII	CCGG	2 cut(s)	978, 1870
HphI	GGTGA	3 cut(s)	766, 1555, 1679
Hpy166II	GTNNAC	7 cut(s)	10, 184, 274, 409, 742, 1737, 2197
Hpy8I	GTNNAC	7 cut(s)	10, 184, 274, 409, 742, 1737, 2197
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	9 cut(s)	90, 160, 194, 476, 803, 855, 1213, 1373, 1403
HpyCH4III	ACNGT	4 cut(s)	859, 1138, 1184, 2148
HpyCH4IV	ACGT	1 cut(s)	15
HpyF10VI	GCNNNNNNNGC	7 cut(s)	664, 707, 721, 911, 1843, 1866, 1887
HpyF3I	CTNAG	8 cut(s)	234, 423, 673, 702, 996, 1660, 1704, 2153
HpySE526I	ACGT	1 cut(s)	15
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	5 cut(s)	172, 221, 878, 893, 1291
HspAI	GCGC	1 cut(s)	300
Kzo9I	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
LmnI	GCTCC	4 cut(s)	369, 563, 1281, 1905
Lsp1109I	GCAGC	6 cut(s)	217, 694, 1111, 1296, 1564, 2176
LweI	GCATC	6 cut(s)	181, 460, 532, 629, 1087, 1180
MaeI	CTAG	8 cut(s)	393, 504, 1037, 1212, 1319, 1340, 1956, 1982
MaeII	ACGT	1 cut(s)	15
MaeIII	GTNAC	4 cut(s)	634, 1132, 1412, 2047
MalI	GATC	6 cut(s)	51, 156, 495, 843, 1032, 1458
MboI	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
MflI	RGATCY	2 cut(s)	49, 1030
MhlI	GDGCHC	3 cut(s)	984, 1278, 1405
MlyI	GAGTC	4 cut(s)	587, 2048, 2091, 2152
MmeI	TCCRAC	6 cut(s)	708, 753, 1628, 1791, 1950, 2118
Mph1103I	ATGCAT	2 cut(s)	174, 1007
MroXI	GAANNNNTTC	3 cut(s)	204, 1383, 1674
MslI	CAYNNNNRTG	1 cut(s)	1694
MspA1I	CMGCKG	3 cut(s)	1708, 1966, 2235
MspCI	CTTAAG	2 cut(s)	719, 800
MspI	CCGG	2 cut(s)	978, 1870
MspR9I	CCNGG	4 cut(s)	605, 979, 1635, 1871
MvaI	CCWGG	2 cut(s)	605, 1635
MvnI	CGCG	3 cut(s)	302, 921, 1773
MwoI	GCNNNNNNNGC	7 cut(s)	664, 707, 721, 911, 1843, 1866, 1887
NciI	CCSGG	2 cut(s)	979, 1871
NdeI	CATATG	3 cut(s)	535, 586, 1782
NdeII	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
NlaIII	CATG	5 cut(s)	172, 221, 878, 893, 1291
NlaIV	GGNNCC	4 cut(s)	1373, 1621, 1754, 1767
NmuCI	GTSAC	2 cut(s)	634, 1412
NsiI	ATGCAT	2 cut(s)	174, 1007
NspI	RCATGY	2 cut(s)	172, 221
PciI	ACATGT	1 cut(s)	217
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 2048
PdmI	GAANNNNTTC	3 cut(s)	204, 1383, 1674
PfeI	GAWTC	7 cut(s)	244, 677, 683, 833, 935, 1560, 2121
PflMI	CCANNNNNTGG	1 cut(s)	1969
PfoI	TCCNGGA	2 cut(s)	603, 1633
PkrI	GCNGC	8 cut(s)	232, 709, 1126, 1286, 1579, 2191, 2231, 2234
PleI	GAGTC	4 cut(s)	587, 2047, 2091, 2152
PpsI	GAGTC	4 cut(s)	587, 2047, 2091, 2152
PscI	ACATGT	1 cut(s)	217
Psp6I	CCWGG	2 cut(s)	603, 1633
PspFI	CCCAGC	1 cut(s)	1499
PspGI	CCWGG	2 cut(s)	603, 1633
PspN4I	GGNNCC	4 cut(s)	1373, 1621, 1754, 1767
PspPI	GGNCC	4 cut(s)	1372, 1519, 1619, 1753
PstNI	CAGNNNCTG	3 cut(s)	680, 863, 2235
PsuI	RGATCY	2 cut(s)	49, 1030
PvuII	CAGCTG	2 cut(s)	1966, 2235
RsaI	GTAC	4 cut(s)	119, 167, 183, 1068
RsaNI	GTAC	4 cut(s)	118, 166, 182, 1067
RseI	CAYNNNNRTG	1 cut(s)	1694
SatI	GCNGC	8 cut(s)	231, 708, 1125, 1285, 1578, 2190, 2230, 2233
Sau3AI	GATC	6 cut(s)	49, 154, 493, 841, 1030, 1456
Sau96I	GGNCC	4 cut(s)	1372, 1519, 1619, 1753
SchI	GAGTC	4 cut(s)	587, 2048, 2091, 2152
ScrFI	CCNGG	4 cut(s)	605, 979, 1635, 1871
SduI	GDGCHC	3 cut(s)	984, 1278, 1405
SfaNI	GCATC	6 cut(s)	181, 460, 532, 629, 1087, 1180
SfcI	CTRYAG	1 cut(s)	209
SinI	GGWCC	3 cut(s)	1372, 1619, 1753
SmiMI	CAYNNNNRTG	1 cut(s)	1694
SmlI	CTYRAG	4 cut(s)	368, 719, 764, 800
SmoI	CTYRAG	4 cut(s)	368, 719, 764, 800
SsiI	CCGC	7 cut(s)	899, 1260, 1309, 1657, 1664, 1708, 1771
SspI	AATATT	4 cut(s)	334, 576, 927, 2013
SspMI	CTAG	8 cut(s)	393, 504, 1037, 1212, 1319, 1340, 1956, 1982
StyD4I	CCNGG	4 cut(s)	603, 977, 1633, 1869
StyI	CCWWGG	3 cut(s)	1279, 1849, 2055
TaaI	ACNGT	4 cut(s)	859, 1138, 1184, 2148
TaiI	ACGT	1 cut(s)	18
TaqI	TCGA	3 cut(s)	59, 129, 153
TaqII	GACCGA	1 cut(s)	1926
TatI	WGTACW	2 cut(s)	165, 1066
TfiI	GAWTC	7 cut(s)	244, 677, 683, 833, 935, 1560, 2121
TscAI	CASTG	5 cut(s)	27, 834, 1000, 1278, 1897
TseFI	GTSAC	2 cut(s)	634, 1412
TseI	GCWGC	8 cut(s)	230, 707, 1124, 1284, 1577, 2189, 2229, 2232
Tsp45I	GTSAC	2 cut(s)	634, 1412
TspDTI	ATGAA	7 cut(s)	338, 475, 1525, 1618, 1626, 1769, 2199
TspGWI	ACGGA	2 cut(s)	899, 1899
TspRI	CASTG	5 cut(s)	27, 834, 1000, 1278, 1897
Van91I	CCANNNNNTGG	1 cut(s)	1969
Vha464I	CTTAAG	2 cut(s)	719, 800
VpaK11BI	GGWCC	3 cut(s)	1372, 1619, 1753
XapI	RAATTY	4 cut(s)	652, 1160, 2087, 2130
XbaI	TCTAGA	2 cut(s)	392, 1318
XceI	RCATGY	2 cut(s)	172, 221
XmiI	GTMKAC	1 cut(s)	273
XmnI	GAANNNNTTC	3 cut(s)	204, 1383, 1674
XspI	CTAG	8 cut(s)	393, 504, 1037, 1212, 1319, 1340, 1956, 1982
Zsp2I	ATGCAT	2 cut(s)	174, 1007

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.