pycom01g09960

1-aminocyclopropane-1-carboxylate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011827
Physical Location & Seq
Reverse (-)
10765634 .. 10768378
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09960.2

Sequence Viewer

Length: 1440 bp
ATGAAAAACATGGTGAGAACTCGCCGTACTTCGACGGATGGAAGGCGTACGATCGATCAAAACCCATTTCACCTAACTGAAAATCCTGAAGGAGTTATCCAGATGGGTCTGGCAGAAAATCAGCTTTCCTTCGACTTGGTTGAAGAGTGGATTAGAAAAAATCCCAAAGCCTCTATTTGCACTGCTGAAGGAATTGAGAAGTTCAGAAGCGTGGCCAATTTTCAAGACTATCATGGCTTACCAGAGTTCAGACAGGCCATTGCTACGTTCATGTCGAAAGCAAGAGGTGGTAGAGTCACATTTGATCCTCATCGCGTAGTTATGAGTGGAGGAGCCACCGGAGCAAATGAGCTGGTCATGTTCTGTTTGGCCAACCGCGGCGATGCTTTCCTTATCCCCTCACCGTACTATCCAGCATTTTACCGAGACCTCGGATGGAGAACTGGAGTCCAAATTGTCCCGGTCGATTGTGATAGCTCCAACAATTTCAAAATAACCAAGGAAGCACTGGAAGCAGCTTATGAAAAAGCCCAAAACAACAACATCAATGTCAAGGGCTTGATCATAACAAACCCATCAAATCCATTAGGCACAACCCTAGACAAAGACACACTTGAAAGCCTTGTCACATTTATAAACCAAAAGAACATTCACTTGGTTTGTGATGAAATCTATGCAGCCACAGTCTTCAGCTCCCCAAAATTCACATGCATCACCGAGGTTATACAAAACATGAATTGCAACCCCAACCTAATCCACATTGTCTACAGTTTGTCCAAGGACATGGGGTTCCCGGGATTGAGAGTCGGCATCGTTTACTCCTACAACGATGACGTGGTGAACATTGGCCGAAAAATGTCAAGTTTTGGGCTGGTCTCATCCCAAACTCAGCACATGCTTGCGTCCATGCTTTCGGACGAAGATTTTGTCGAGAAGTTCCTCACCACAAGCGCAAAAAGGCTCGCAAAGAGGCACGGGGTCTTCACTGATGGGCTTGAGGAAGTGGGAATCAACTGCTTGAAGAGCAATGCCGGCCTTTTCTGTTGGATGGACTTAAGGAGGCTATTGAAAGATCAAACGTTTGATGGCGAAATGGTGTTGTGGCGTGTAATCGTTAATGAAGTGAAGCTCAACGTTTCTCCGGGCTCTTCATTTAAATGTGTCGAGCCTGGTTGGTTTAGGGTTTGCTTCGCCAACATGGATGATGACACAGTGGAAGTTGCACTCAAAAGGATTAGGGCATTTGTAGGGCAAGGAAAGAAAGCTCAAGAACAAGCACAAGTGAAAAGTCCTAAGAAGCGTTGGCAGAGCAATCTAAGGCTGAGCTTTTCATCATCATCCAGAAGGTTTGAGCAGGAAGGTGTTAGTGTTATGTCACCACGTATGATGTCTCCTCATTCACCAATGCCTCACTCACCTCTCCTTCGAGCTACGACTTAA

Protein Analysis

480

Amino Acids

53.83

Weight (kDa)

8.8

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01480 AT1G01480 AT3G61510
fragaria_vesca FvH4_7g15310
malus_domestica MD00G1167500.v1.1 MD01G1070400.v1.1
prunus_persica Prupe.2G176900_v2.0.a1
pyrus_communis pycom01g09960 pycom07g12870
rosa_chinensis RchiOBHm_Chr1g0357641
rosa_laevigata RLG00000028012
rosa_multiflora Rmu_sc0004683.1_g000005
rosa_roxburghii Rroxscaffold_4G00298680
rosa_rugosa Rorug01G0257300
rosa_samantha Rh1AG271300 Rh1BG238200 Rh1CG255100 Rh1DG266500
rosa_wichuraiana Rw1G024180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AccI GTMKAC 1 cut(s) 765
AccII CGCG 2 cut(s) 315, 378
AciI CCGC 2 cut(s) 376, 378
AclI AACGTT 2 cut(s) 1079, 1134
AclWI GGATC 1 cut(s) 299
AcoI YGGCCR 3 cut(s) 213, 369, 847
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 3 cut(s) 108, 207, 673
AfaI GTAC 3 cut(s) 28, 49, 407
AflII CTTAAG 1 cut(s) 1054
AgsI TTSAA 6 cut(s) 143, 224, 490, 617, 1021, 1069
AjiI CACGTC 1 cut(s) 835
AjnI CCWGG 1 cut(s) 1168
AjuI GAANNNNNNNTTGG 4 cut(s) 633, 638, 665, 670
AloI GAACNNNNNNTCC 2 cut(s) 773, 805
AluBI AGCT 9 cut(s) 124, 352, 477, 518, 693, 1129, 1265, 1326, 1430
AluI AGCT 9 cut(s) 124, 352, 477, 518, 693, 1129, 1265, 1326, 1430
Alw26I GTCTC 3 cut(s) 420, 880, 1395
AlwI GGATC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 793
AoxI GGCC 5 cut(s) 213, 255, 369, 847, 1033
ApeKI GCWGC 2 cut(s) 515, 677
ApoI RAATTY 1 cut(s) 701
ArsI GACNNNNNNTTYG 4 cut(s) 908, 912, 940, 944
AspLEI GCGC 1 cut(s) 953
AsuC2I CCSGG 4 cut(s) 461, 794, 795, 1143
AsuHPI GGTGA 9 cut(s) 25, 62, 393, 706, 850, 934, 1368, 1392, 1407
AvaI CYCGRG 1 cut(s) 793
BalI TGGCCA 2 cut(s) 215, 371
BanII GRGCYC 1 cut(s) 1148
BbsI GAAGAC 2 cut(s) 679, 973
BbvI GCAGC 2 cut(s) 527, 689
BccI CCATC 7 cut(s) 32, 97, 429, 583, 983, 1042, 1079
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 1 cut(s) 1170
BclI TGATCA 1 cut(s) 561
BcnI CCSGG 4 cut(s) 461, 794, 795, 1143
BcoDI GTCTC 3 cut(s) 420, 880, 1395
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 766
BfrI CTTAAG 1 cut(s) 1054
BisI GCNGC 3 cut(s) 379, 516, 678
BlpI GCTNAGC 1 cut(s) 1322
BlsI GCNGC 3 cut(s) 380, 517, 679
Bme1390I CCNGG 5 cut(s) 461, 794, 795, 1143, 1170
BmeT110I CYCGRG 1 cut(s) 793
BmgBI CACGTC 1 cut(s) 835
BmiI GGNNCC 2 cut(s) 334, 791
BmrFI CCNGG 5 cut(s) 461, 794, 795, 1143, 1170
BmsI GCATC 3 cut(s) 373, 720, 819
BpiI GAAGAC 2 cut(s) 679, 973
BpmI CTGGAG 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 1322
BpuEI CTTGAG 2 cut(s) 1016, 1251
BpuMI CCSGG 4 cut(s) 461, 794, 795, 1143
Bsa29I ATCGAT 1 cut(s) 54
BsaAI YACGTR 1 cut(s) 1382
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 2 cut(s) 420, 880
BsaJI CCNNGG 6 cut(s) 376, 430, 498, 717, 777, 793
BsaWI WCCGGW 1 cut(s) 338
Bse118I RCCGGY 1 cut(s) 1031
Bse1I ACTGG 2 cut(s) 448, 513
Bse3DI GCAATG 2 cut(s) 258, 1033
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 1170
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 6 cut(s) 376, 430, 498, 717, 777, 793
BseGI GGATG 6 cut(s) 43, 440, 878, 1053, 1207, 1337
BseJI GATNNNNATC 1 cut(s) 309
BseMI GCAATG 2 cut(s) 258, 1033
BseMII CTCAG 2 cut(s) 902, 1313
BseNI ACTGG 2 cut(s) 448, 513
BseRI GAGGAG 2 cut(s) 345, 1383
BseXI GCAGC 2 cut(s) 527, 689
Bsh1236I CGCG 2 cut(s) 315, 378
Bsh1285I CGRYCG 2 cut(s) 54, 465
BshFI GGCC 5 cut(s) 215, 257, 371, 849, 1035
BshVI ATCGAT 1 cut(s) 54
BsiEI CGRYCG 2 cut(s) 54, 465
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 5 cut(s) 339, 461, 794, 1032, 1142
BsiWI CGTACG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 443
BsmAI GTCTC 3 cut(s) 420, 880, 1395
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 5 cut(s) 215, 257, 371, 849, 1035
Bso31I GGTCTC 2 cut(s) 420, 880
BsoBI CYCGRG 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 1148
Bsp143I GATC 5 cut(s) 51, 55, 304, 561, 1072
Bsp1720I GCTNAGC 1 cut(s) 1322
BspACI CCGC 2 cut(s) 376, 378
BspANI GGCC 5 cut(s) 215, 257, 371, 849, 1035
BspCNI CTCAG 2 cut(s) 901, 1314
BspDI ATCGAT 1 cut(s) 54
BspFNI CGCG 2 cut(s) 315, 378
BspLI GGNNCC 2 cut(s) 334, 791
BspPI GGATC 1 cut(s) 299
BspQI GCTCTTC 2 cut(s) 1016, 1153
BspTI CTTAAG 1 cut(s) 1054
BspTNI GGTCTC 2 cut(s) 420, 880
BsrDI GCAATG 2 cut(s) 258, 1033
BsrFI RCCGGY 1 cut(s) 1031
BsrI ACTGG 2 cut(s) 448, 513
BssAI RCCGGY 1 cut(s) 1031
BssECI CCNNGG 6 cut(s) 376, 430, 498, 717, 777, 793
BssMI GATC 5 cut(s) 51, 55, 304, 561, 1072
BssT1I CCWWGG 2 cut(s) 498, 777
Bst2UI CCWGG 1 cut(s) 1170
Bst4CI ACNGT 4 cut(s) 405, 685, 770, 1213
Bst6I CTCTTC 3 cut(s) 138, 1016, 1153
BstAFI CTTAAG 1 cut(s) 1054
BstBAI YACGTR 1 cut(s) 1382
BstC8I GCNNGC 3 cut(s) 900, 963, 1033
BstDEI CTNAG 4 cut(s) 888, 1293, 1316, 1322
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 6 cut(s) 43, 440, 878, 1053, 1207, 1337
BstFNI CGCG 2 cut(s) 315, 378
BstHHI GCGC 1 cut(s) 953
BstKTI GATC 5 cut(s) 54, 58, 307, 564, 1075
BstMAI GTCTC 3 cut(s) 420, 880, 1395
BstMBI GATC 5 cut(s) 51, 55, 304, 561, 1072
BstMCI CGRYCG 2 cut(s) 54, 465
BstMWI GCNNNNNNNGC 4 cut(s) 341, 512, 1023, 1032
BstNI CCWGG 1 cut(s) 1170
BstNSI RCATGY 2 cut(s) 711, 898
BstSCI CCNGG 5 cut(s) 459, 792, 793, 1141, 1168
BstSFI CTRYAG 1 cut(s) 766
BstUI CGCG 2 cut(s) 315, 378
BstV1I GCAGC 2 cut(s) 527, 689
BstV2I GAAGAC 2 cut(s) 679, 973
BstXI CCANNNNNNTGG 1 cut(s) 784
Bsu15I ATCGAT 1 cut(s) 54
BsuRI GGCC 5 cut(s) 215, 257, 371, 849, 1035
BsuTUI ATCGAT 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 376
BtgZI GCGATG 2 cut(s) 296, 396
BtrI CACGTC 1 cut(s) 835
BtsCI GGATG 6 cut(s) 43, 440, 878, 1053, 1207, 1337
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 4 cut(s) 180, 506, 984, 1218
Cac8I GCNNGC 3 cut(s) 900, 963, 1033
CfoI GCGC 1 cut(s) 953
Cfr10I RCCGGY 1 cut(s) 1031
Cfr42I CCGCGG 1 cut(s) 379
Cfr9I CCCGGG 1 cut(s) 793
ClaI ATCGAT 1 cut(s) 54
CseI GACGC 1 cut(s) 891
Csp6I GTAC 3 cut(s) 27, 48, 406
CviQI GTAC 3 cut(s) 27, 48, 406
DdeI CTNAG 4 cut(s) 888, 1293, 1316, 1322
DpnI GATC 5 cut(s) 53, 57, 306, 563, 1074
DpnII GATC 5 cut(s) 51, 55, 304, 561, 1072
DraI TTTAAA 1 cut(s) 1156
EaeI YGGCCR 3 cut(s) 213, 369, 847
Eam1104I CTCTTC 3 cut(s) 138, 1016, 1153
EarI CTCTTC 3 cut(s) 138, 1016, 1153
Eco130I CCWWGG 2 cut(s) 498, 777
Eco24I GRGCYC 1 cut(s) 1148
Eco31I GGTCTC 2 cut(s) 420, 880
Eco57I CTGAAG 3 cut(s) 108, 207, 673
Eco88I CYCGRG 1 cut(s) 793
EcoRII CCWGG 1 cut(s) 1168
EcoT14I CCWWGG 2 cut(s) 498, 777
EcoT22I ATGCAT 1 cut(s) 713
EcoT38I GRGCYC 1 cut(s) 1148
ErhI CCWWGG 2 cut(s) 498, 777
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 1 cut(s) 443
FbaI TGATCA 1 cut(s) 561
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 3 cut(s) 379, 516, 678
FokI GGATG 6 cut(s) 50, 447, 865, 1060, 1214, 1324
FriOI GRGCYC 1 cut(s) 1148
Fsp4HI GCNGC 3 cut(s) 379, 516, 678
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 952
GluI GCNGC 3 cut(s) 379, 516, 678
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 5 cut(s) 215, 257, 371, 849, 1035
HapII CCGG 5 cut(s) 339, 461, 794, 1032, 1142
HgaI GACGC 1 cut(s) 891
HhaI GCGC 1 cut(s) 953
Hin6I GCGC 1 cut(s) 951
HinP1I GCGC 1 cut(s) 951
HinfI GANTC 4 cut(s) 294, 447, 804, 1008
HpaII CCGG 5 cut(s) 339, 461, 794, 1032, 1142
HphI GGTGA 9 cut(s) 25, 62, 393, 706, 850, 934, 1368, 1392, 1407
Hpy166II GTNNAC 3 cut(s) 766, 817, 841
Hpy188I TCNGA 4 cut(s) 206, 251, 434, 916
Hpy188III TCNNGA 6 cut(s) 86, 100, 224, 931, 1268, 1341
Hpy8I GTNNAC 3 cut(s) 766, 817, 841
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 7 cut(s) 36, 83, 139, 182, 1338, 1352, 1433
HpyCH4III ACNGT 4 cut(s) 405, 685, 770, 1213
HpyCH4IV ACGT 5 cut(s) 266, 834, 1079, 1134, 1381
HpyCH4V TGCA 5 cut(s) 180, 677, 711, 741, 1223
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 512, 1023, 1032
HpyF3I CTNAG 4 cut(s) 888, 1293, 1316, 1322
HpySE526I ACGT 5 cut(s) 266, 834, 1079, 1134, 1381
HspAI GCGC 1 cut(s) 951
KroI GCCGGC 1 cut(s) 1031
KroNI GCCGGC 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 561
KspI CCGCGG 1 cut(s) 379
Kzo9I GATC 5 cut(s) 51, 55, 304, 561, 1072
LguI GCTCTTC 2 cut(s) 1016, 1153
LmnI GCTCC 4 cut(s) 332, 341, 482, 698
Lsp1109I GCAGC 2 cut(s) 527, 689
LweI GCATC 3 cut(s) 373, 720, 819
MaeI CTAG 1 cut(s) 599
MaeII ACGT 5 cut(s) 266, 834, 1079, 1134, 1381
MaeIII GTNAC 3 cut(s) 295, 625, 1374
MalI GATC 5 cut(s) 53, 57, 306, 563, 1074
MboI GATC 5 cut(s) 51, 55, 304, 561, 1072
MboII GAAGA 6 cut(s) 155, 679, 932, 973, 1033, 1140
MhlI GDGCHC 1 cut(s) 1148
MlsI TGGCCA 2 cut(s) 215, 371
MluCI AATT 6 cut(s) 192, 217, 453, 484, 701, 736
MluNI TGGCCA 2 cut(s) 215, 371
MlyI GAGTC 3 cut(s) 303, 456, 813
MmeI TCCRAC 2 cut(s) 504, 1025
Mox20I TGGCCA 2 cut(s) 215, 371
Mph1103I ATGCAT 1 cut(s) 713
MroNI GCCGGC 1 cut(s) 1031
MscI TGGCCA 2 cut(s) 215, 371
MseI TTAA 4 cut(s) 1055, 1116, 1155, 1438
MslI CAYNNNNRTG 1 cut(s) 1156
Msp20I TGGCCA 2 cut(s) 215, 371
MspA1I CMGCKG 1 cut(s) 378
MspCI CTTAAG 1 cut(s) 1054
MspI CCGG 5 cut(s) 339, 461, 794, 1032, 1142
MspR9I CCNGG 5 cut(s) 461, 794, 795, 1143, 1170
MvaI CCWGG 1 cut(s) 1170
MvnI CGCG 2 cut(s) 315, 378
MwoI GCNNNNNNNGC 4 cut(s) 341, 512, 1023, 1032
NaeI GCCGGC 1 cut(s) 1033
NciI CCSGG 4 cut(s) 461, 794, 795, 1143
NdeII GATC 5 cut(s) 51, 55, 304, 561, 1072
NgoMIV GCCGGC 1 cut(s) 1031
NlaIV GGNNCC 2 cut(s) 334, 791
NmuCI GTSAC 3 cut(s) 295, 625, 1374
NsiI ATGCAT 1 cut(s) 713
NspI RCATGY 2 cut(s) 711, 898
PciSI GCTCTTC 2 cut(s) 1016, 1153
PcsI WCGNNNNNNNCGW 1 cut(s) 272
PdiI GCCGGC 1 cut(s) 1033
PfeI GAWTC 1 cut(s) 1008
Pfl23II CGTACG 1 cut(s) 47
PkrI GCNGC 3 cut(s) 380, 517, 679
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 3 cut(s) 302, 455, 812
PpsI GAGTC 3 cut(s) 302, 455, 812
Ppu21I YACGTR 1 cut(s) 1382
PsiI TTATAA 1 cut(s) 635
Psp1406I AACGTT 2 cut(s) 1079, 1134
Psp6I CCWGG 1 cut(s) 1168
PspGI CCWGG 1 cut(s) 1168
PspLI CGTACG 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 334, 791
PsrI GAACNNNNNNTAC 2 cut(s) 10, 42
PvuI CGATCG 1 cut(s) 54
RsaI GTAC 3 cut(s) 28, 49, 407
RsaNI GTAC 3 cut(s) 27, 48, 406
RseI CAYNNNNRTG 1 cut(s) 1156
SacII CCGCGG 1 cut(s) 379
SapI GCTCTTC 2 cut(s) 1016, 1153
SaqAI TTAA 4 cut(s) 1055, 1116, 1155, 1438
SatI GCNGC 3 cut(s) 379, 516, 678
Sau3AI GATC 5 cut(s) 51, 55, 304, 561, 1072
SchI GAGTC 3 cut(s) 303, 456, 813
ScrFI CCNGG 5 cut(s) 461, 794, 795, 1143, 1170
SduI GDGCHC 1 cut(s) 1148
SfaNI GCATC 3 cut(s) 373, 720, 819
SfcI CTRYAG 1 cut(s) 766
Sfr303I CCGCGG 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 379
SmaI CCCGGG 1 cut(s) 795
SmiI ATTTAAAT 1 cut(s) 1156
SmiMI CAYNNNNRTG 1 cut(s) 1156
SmlI CTYRAG 3 cut(s) 995, 1054, 1266
SmoI CTYRAG 3 cut(s) 995, 1054, 1266
Sse9I AATT 6 cut(s) 192, 217, 453, 484, 701, 736
SsiI CCGC 2 cut(s) 376, 378
SspMI CTAG 1 cut(s) 599
StyD4I CCNGG 5 cut(s) 459, 792, 793, 1141, 1168
StyI CCWWGG 2 cut(s) 498, 777
SwaI ATTTAAAT 1 cut(s) 1156
TaaI ACNGT 4 cut(s) 405, 685, 770, 1213
TaiI ACGT 5 cut(s) 269, 837, 1082, 1137, 1384
TaqI TCGA 8 cut(s) 32, 54, 132, 275, 465, 930, 1164, 1426
TasI AATT 6 cut(s) 192, 217, 453, 484, 701, 736
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 1 cut(s) 1008
Tru1I TTAA 4 cut(s) 1055, 1116, 1155, 1438
Tru9I TTAA 4 cut(s) 1055, 1116, 1155, 1438
TscAI CASTG 4 cut(s) 187, 513, 991, 1218
TseFI GTSAC 3 cut(s) 295, 625, 1374
TseI GCWGC 2 cut(s) 515, 677
Tsp45I GTSAC 3 cut(s) 295, 625, 1374
TspDTI ATGAA 8 cut(s) 17, 259, 537, 681, 749, 1134, 1140, 1320
TspGWI ACGGA 1 cut(s) 50
TspMI CCCGGG 1 cut(s) 793
TspRI CASTG 4 cut(s) 187, 513, 991, 1218
Vha464I CTTAAG 1 cut(s) 1054
XapI RAATTY 1 cut(s) 701
XceI RCATGY 2 cut(s) 711, 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmaI CCCGGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 765
XspI CTAG 1 cut(s) 599
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.