pycom07g12870

1-aminocyclopropane-1-carboxylate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011827
Physical Location & Seq
Forward (+)
14253036 .. 14256933
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12870.1

Sequence Viewer

Length: 1572 bp
ATGTATTATATAAGGATCACCAACAGGTACACTACTGGTGTACACGTGACAACATGCAAGCTTTACGACTCATTGACCAACAAAATGGCATCATCTGCAAGTAAAAATAGCTTACTGCTATCGAAGATTGCAACCAATGAAAAACATGGTGAGAACTCGCCGTACTTCAACGGATGGAAGGCGTACGATCAAAACCCTTTTCACCCAACTGAAAACCCTGAGGGTGTAATCCAGATGGGCCTTGCAGAAAATCAGCTTTCCTTTGATTTGGTTGAAGAATGGATTAGGAAAAATCCCAAAGCCTCTTTTTGCACTGCTGAAGGAGTTGAGAAGTTCAGAAGCGTGGCCAATTTTCAAGATTATCATGGCTTCCCAGAGTTCAGACAGGCCATTGCTACGTTCATGTCGAAAGCAAGAGGTGGCAGAGTCACGTTTGATCCTGACCGCGTAGTGATGAGTGGCGGAGCCACCGGAGCAAACGAGCTGGTCATGTTCTGTTTGGCTGACCCCGGCGATGCTTTCCTTATCCCCTCACCGTACTATCCAGCATTTTACCGAGACCTCGGATGGAGAACTGGAGTCCAAATTGTCCCAGTCGATTGTGATAGCTCCAACAATTTCAAAATAACCAAAGAAGCAGTGGAAGCAGCTTACGAAAAAGCCAAAAACAACAACATCAAAGTGAAGGGCTTGATCATAACAAACCCATCAAATCCATTGGGCACAACCCTTGACAAAGACACACTTGAAAGCCTTGTCACATTCATAAACCAAAAGAACATTCACTTGGTTTGTGATGAAATCTATGCAGCCACAGTCTTCAGCTCCCCAAAATACACATGCATCACCGAGGTCATACAAGACATGGATTGCAATCCCAACCTAATCCACATTGTCTACAGTTTGTCCAAGGACATGGGGTTTCCCGGTTTGAGGGTCGGCATTGTTTACTCCTACAACGATGATGTGGTGAACATCGGGCGAAAAATGTCAAGTTTCGGGCTGGTCTCATCCCAAACACAACACATGCTCGCATCAATGCTATCGGATGAAGATTTTGTCGAGAAGTTCCTCGCGACAAGCTCAAAAAGGCTTGCGAAGAGGCACGGGGTCTTCACGAAGGGGCTTGAGGAAGTGGGAATTAGCTATTTGAAGAGCAATGCAGGCCTCTTCTGTTGGATGGACTTAAGGAGGCTGTTGAAAGATCAAACGCTCGACGGAGAAATGGTGTTGTGGCGTGTGATTGTGAACGAAGTGAAGCTCAACATTTCGCCGGGCTCTTCGTTTAAATGTGTGGAGCCTGGTTGGTTTAGGGTTTGCTTTGCCAACATGGATGATGACACTGTGGAAGTTGCACTCAAAAGGATTAGGGCACTCGTAGGGCAAGAAAAGAAAGCTCAAGAACAAGCACAAGTGAAAAGTACTAAGAAGCGTTGGCAGAGCAATCTAAGGCTGAGCTTTTCATCGTCAATAAGAAGATTTGAGCAGGAAGGTGTTAGTGTTAAGTCACCACACATGATGTCTCCACATTCACCAATGCCTCACACGCCTTTCGTTCGAGCTACGACTTAA

Protein Analysis

524

Amino Acids

58.8

Weight (kDa)

8.5

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 77 - 456 3.1e-108 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01480 AT1G01480 AT3G61510
fragaria_vesca FvH4_7g15310
malus_domestica MD00G1167500.v1.1 MD01G1070400.v1.1
prunus_persica Prupe.2G176900_v2.0.a1
pyrus_communis pycom01g09960 pycom07g12870
rosa_chinensis RchiOBHm_Chr1g0357641
rosa_laevigata RLG00000028012
rosa_multiflora Rmu_sc0004683.1_g000005
rosa_roxburghii Rroxscaffold_4G00298680
rosa_rugosa Rorug01G0257300
rosa_samantha Rh1AG271300 Rh1BG238200 Rh1CG255100 Rh1DG266500
rosa_wichuraiana Rw1G024180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 897
AccII CGCG 2 cut(s) 447, 1076
AciI CCGC 2 cut(s) 445, 462
AclWI GGATC 2 cut(s) 23, 431
AcoI YGGCCR 1 cut(s) 345
AcuI CTGAAG 2 cut(s) 339, 805
AcvI CACGTG 1 cut(s) 46
AfaI GTAC 6 cut(s) 29, 42, 164, 185, 539, 1423
AfiI CCNNNNNNNGG 1 cut(s) 933
AflII CTTAAG 1 cut(s) 1186
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 7 cut(s) 169, 275, 356, 622, 749, 1153, 1201
AjnI CCWGG 1 cut(s) 1300
AjuI GAANNNNNNNTTGG 4 cut(s) 765, 770, 797, 802
Alw26I GTCTC 3 cut(s) 552, 1012, 1527
AlwI GGATC 2 cut(s) 23, 431
AoxI GGCC 4 cut(s) 238, 345, 387, 1165
ApeKI GCWGC 2 cut(s) 647, 809
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 3 cut(s) 510, 927, 1275
AsuHPI GGTGA 8 cut(s) 10, 161, 194, 525, 838, 982, 1500, 1524
AxyI CCTNAGG 1 cut(s) 219
BaeGI GKGCMC 2 cut(s) 725, 1375
BalI TGGCCA 1 cut(s) 347
BanII GRGCYC 1 cut(s) 1280
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 811, 1105
BbvI GCAGC 2 cut(s) 659, 821
BccI CCATC 5 cut(s) 168, 229, 561, 715, 1174
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 1302
BclI TGATCA 1 cut(s) 693
BcnI CCSGG 3 cut(s) 510, 927, 1275
BcoDI GTCTC 3 cut(s) 552, 1012, 1527
BfmI CTRYAG 1 cut(s) 898
BfrI CTTAAG 1 cut(s) 1186
BisI GCNGC 2 cut(s) 648, 810
BlpI GCTNAGC 1 cut(s) 1454
BlsI GCNGC 2 cut(s) 649, 811
BmcAI AGTACT 1 cut(s) 1423
Bme1390I CCNGG 4 cut(s) 510, 927, 1275, 1302
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 466, 1299
BmrFI CCNGG 4 cut(s) 510, 927, 1275, 1302
BmrI ACTGGG 1 cut(s) 587
BmsI GCATC 4 cut(s) 98, 505, 852, 1043
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 2 cut(s) 811, 1105
BpmI CTGGAG 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 1454
BpuEI CTTGAG 2 cut(s) 1148, 1383
BpuMI CCSGG 3 cut(s) 510, 927, 1275
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 873
BsaI GGTCTC 2 cut(s) 552, 1012
BsaJI CCNNGG 4 cut(s) 508, 562, 849, 909
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 2 cut(s) 1212, 1242
Bsc4I CCNNNNNNNGG 1 cut(s) 933
Bse1I ACTGG 3 cut(s) 40, 580, 593
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 390, 1165
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 1 cut(s) 1302
BseDI CCNNGG 4 cut(s) 508, 562, 849, 909
BseGI GGATG 6 cut(s) 179, 572, 1010, 1054, 1185, 1339
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 1 cut(s) 933
BseMI GCAATG 2 cut(s) 390, 1165
BseMII CTCAG 2 cut(s) 210, 1445
BseNI ACTGG 3 cut(s) 40, 580, 593
BseSI GKGCMC 2 cut(s) 725, 1375
BseXI GCAGC 2 cut(s) 659, 821
Bsh1236I CGCG 2 cut(s) 447, 1076
BshFI GGCC 4 cut(s) 240, 347, 389, 1167
BsiSI CCGG 4 cut(s) 471, 510, 927, 1274
BsiWI CGTACG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 1 cut(s) 933
BsmAI GTCTC 3 cut(s) 552, 1012, 1527
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 4 cut(s) 240, 347, 389, 1167
Bso31I GGTCTC 2 cut(s) 552, 1012
Bsp1286I GDGCHC 3 cut(s) 725, 1280, 1375
Bsp1407I TGTACA 1 cut(s) 40
Bsp143I GATC 5 cut(s) 15, 187, 436, 693, 1204
Bsp1720I GCTNAGC 1 cut(s) 1454
Bsp68I TCGCGA 1 cut(s) 1076
BspACI CCGC 2 cut(s) 445, 462
BspANI GGCC 4 cut(s) 240, 347, 389, 1167
BspCNI CTCAG 2 cut(s) 211, 1446
BspFNI CGCG 2 cut(s) 447, 1076
BspLI GGNNCC 2 cut(s) 466, 1299
BspPI GGATC 2 cut(s) 23, 431
BspQI GCTCTTC 2 cut(s) 1148, 1285
BspTI CTTAAG 1 cut(s) 1186
BspTNI GGTCTC 2 cut(s) 552, 1012
BsrDI GCAATG 2 cut(s) 390, 1165
BsrGI TGTACA 1 cut(s) 40
BsrI ACTGG 3 cut(s) 40, 580, 593
BssECI CCNNGG 4 cut(s) 508, 562, 849, 909
BssMI GATC 5 cut(s) 15, 187, 436, 693, 1204
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 1 cut(s) 1302
Bst4CI ACNGT 4 cut(s) 537, 817, 902, 1345
Bst6I CTCTTC 4 cut(s) 1094, 1148, 1175, 1285
BstAFI CTTAAG 1 cut(s) 1186
BstAPI GCANNNNNTGC 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 59, 1032, 1095, 1165
BstDEI CTNAG 4 cut(s) 219, 1425, 1448, 1454
BstF5I GGATG 6 cut(s) 179, 572, 1010, 1054, 1185, 1339
BstFNI CGCG 2 cut(s) 447, 1076
BstKTI GATC 5 cut(s) 18, 190, 439, 696, 1207
BstMAI GTCTC 3 cut(s) 552, 1012, 1527
BstMBI GATC 5 cut(s) 15, 187, 436, 693, 1204
BstMWI GCNNNNNNNGC 5 cut(s) 95, 473, 644, 1164, 1546
BstNI CCWGG 1 cut(s) 1302
BstNSI RCATGY 3 cut(s) 57, 843, 1030
BstSCI CCNGG 4 cut(s) 508, 925, 1273, 1300
BstSFI CTRYAG 1 cut(s) 898
BstSLI GKGCMC 2 cut(s) 725, 1375
BstUI CGCG 2 cut(s) 447, 1076
BstV1I GCAGC 2 cut(s) 659, 821
BstV2I GAAGAC 2 cut(s) 811, 1105
BstXI CCANNNNNNTGG 2 cut(s) 85, 916
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 4 cut(s) 240, 347, 389, 1167
BtgZI GCGATG 1 cut(s) 528
BtsCI GGATG 6 cut(s) 179, 572, 1010, 1054, 1185, 1339
BtsI GCAGTG 2 cut(s) 312, 645
BtsIMutI CAGTG 3 cut(s) 312, 645, 1341
BtuMI TCGCGA 1 cut(s) 1076
Cac8I GCNNGC 4 cut(s) 59, 1032, 1095, 1165
Cfr13I GGNCC 1 cut(s) 238
Csp6I GTAC 6 cut(s) 28, 41, 163, 184, 538, 1422
CviQI GTAC 6 cut(s) 28, 41, 163, 184, 538, 1422
DdeI CTNAG 4 cut(s) 219, 1425, 1448, 1454
DpnI GATC 5 cut(s) 17, 189, 438, 695, 1206
DpnII GATC 5 cut(s) 15, 187, 436, 693, 1204
DraI TTTAAA 1 cut(s) 1288
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 4 cut(s) 1094, 1148, 1175, 1285
EarI CTCTTC 4 cut(s) 1094, 1148, 1175, 1285
EciI GGCGGA 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 909
Eco147I AGGCCT 1 cut(s) 1167
Eco24I GRGCYC 1 cut(s) 1280
Eco31I GGTCTC 2 cut(s) 552, 1012
Eco57I CTGAAG 2 cut(s) 339, 805
Eco72I CACGTG 1 cut(s) 46
Eco81I CCTNAGG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 1300
EcoT14I CCWWGG 1 cut(s) 909
EcoT22I ATGCAT 1 cut(s) 845
EcoT38I GRGCYC 1 cut(s) 1280
ErhI CCWWGG 1 cut(s) 909
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 1 cut(s) 575
FbaI TGATCA 1 cut(s) 693
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 2 cut(s) 648, 810
FokI GGATG 6 cut(s) 186, 579, 997, 1061, 1192, 1346
FriOI GRGCYC 1 cut(s) 1280
Fsp4HI GCNGC 2 cut(s) 648, 810
GluI GCNGC 2 cut(s) 648, 810
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 4 cut(s) 240, 347, 389, 1167
HapII CCGG 4 cut(s) 471, 510, 927, 1274
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 3 cut(s) 68, 426, 579
HpaII CCGG 4 cut(s) 471, 510, 927, 1274
HphI GGTGA 8 cut(s) 10, 161, 194, 525, 838, 982, 1500, 1524
Hpy166II GTNNAC 7 cut(s) 30, 41, 43, 898, 949, 973, 1249
Hpy188I TCNGA 4 cut(s) 338, 383, 566, 1048
Hpy188III TCNNGA 7 cut(s) 232, 356, 440, 1063, 1075, 1117, 1400
Hpy8I GTNNAC 7 cut(s) 30, 41, 43, 898, 949, 973, 1249
Hpy99I CGWCG 1 cut(s) 1220
HpyAV CCTTC 5 cut(s) 172, 314, 679, 1114, 1484
HpyCH4III ACNGT 4 cut(s) 537, 817, 902, 1345
HpyCH4IV ACGT 3 cut(s) 45, 398, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 473, 644, 1164, 1546
HpyF3I CTNAG 4 cut(s) 219, 1425, 1448, 1454
HpySE526I ACGT 3 cut(s) 45, 398, 431
Ksp22I TGATCA 1 cut(s) 693
Kzo9I GATC 5 cut(s) 15, 187, 436, 693, 1204
LguI GCTCTTC 2 cut(s) 1148, 1285
LmnI GCTCC 5 cut(s) 464, 473, 614, 830, 1297
Lsp1109I GCAGC 2 cut(s) 659, 821
LweI GCATC 4 cut(s) 98, 505, 852, 1043
MaeII ACGT 3 cut(s) 45, 398, 431
MaeIII GTNAC 4 cut(s) 46, 427, 757, 1506
MalI GATC 5 cut(s) 17, 189, 438, 695, 1206
MboI GATC 5 cut(s) 15, 187, 436, 693, 1204
MhlI GDGCHC 3 cut(s) 725, 1280, 1375
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 4 cut(s) 349, 585, 616, 1140
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 3 cut(s) 62, 435, 588
MmeI TCCRAC 2 cut(s) 636, 1157
Mox20I TGGCCA 1 cut(s) 347
Mph1103I ATGCAT 1 cut(s) 845
MscI TGGCCA 1 cut(s) 347
MseI TTAA 4 cut(s) 1187, 1287, 1503, 1570
MslI CAYNNNNRTG 1 cut(s) 680
Msp20I TGGCCA 1 cut(s) 347
MspCI CTTAAG 1 cut(s) 1186
MspI CCGG 4 cut(s) 471, 510, 927, 1274
MspR9I CCNGG 4 cut(s) 510, 927, 1275, 1302
MvaI CCWGG 1 cut(s) 1302
MvnI CGCG 2 cut(s) 447, 1076
MwoI GCNNNNNNNGC 5 cut(s) 95, 473, 644, 1164, 1546
NciI CCSGG 3 cut(s) 510, 927, 1275
NdeII GATC 5 cut(s) 15, 187, 436, 693, 1204
NlaIV GGNNCC 2 cut(s) 466, 1299
NmuCI GTSAC 4 cut(s) 46, 427, 757, 1506
NruI TCGCGA 1 cut(s) 1076
NsiI ATGCAT 1 cut(s) 845
NspI RCATGY 3 cut(s) 57, 843, 1030
PceI AGGCCT 1 cut(s) 1167
PciSI GCTCTTC 2 cut(s) 1148, 1285
PcsI WCGNNNNNNNCGW 1 cut(s) 404
Pfl23II CGTACG 1 cut(s) 183
PkrI GCNGC 2 cut(s) 649, 811
PleI GAGTC 3 cut(s) 62, 434, 587
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
PpsI GAGTC 3 cut(s) 62, 434, 587
Ppu21I YACGTR 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 1300
PspCI CACGTG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 1300
PspLI CGTACG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 466, 1299
PspPI GGNCC 1 cut(s) 238
PsrI GAACNNNNNNTAC 2 cut(s) 146, 178
RruI TCGCGA 1 cut(s) 1076
RsaI GTAC 6 cut(s) 29, 42, 164, 185, 539, 1423
RsaNI GTAC 6 cut(s) 28, 41, 163, 184, 538, 1422
RseI CAYNNNNRTG 1 cut(s) 680
SapI GCTCTTC 2 cut(s) 1148, 1285
SaqAI TTAA 4 cut(s) 1187, 1287, 1503, 1570
SatI GCNGC 2 cut(s) 648, 810
Sau3AI GATC 5 cut(s) 15, 187, 436, 693, 1204
Sau96I GGNCC 1 cut(s) 238
ScaI AGTACT 1 cut(s) 1423
SchI GAGTC 3 cut(s) 62, 435, 588
ScrFI CCNGG 4 cut(s) 510, 927, 1275, 1302
SduI GDGCHC 3 cut(s) 725, 1280, 1375
SfaNI GCATC 4 cut(s) 98, 505, 852, 1043
SfcI CTRYAG 1 cut(s) 898
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 3 cut(s) 1127, 1186, 1398
SmoI CTYRAG 3 cut(s) 1127, 1186, 1398
Sse9I AATT 4 cut(s) 349, 585, 616, 1140
SseBI AGGCCT 1 cut(s) 1167
SsiI CCGC 2 cut(s) 445, 462
StuI AGGCCT 1 cut(s) 1167
StyD4I CCNGG 4 cut(s) 508, 925, 1273, 1300
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 4 cut(s) 537, 817, 902, 1345
TaiI ACGT 3 cut(s) 48, 401, 434
TaqI TCGA 6 cut(s) 122, 407, 597, 1062, 1215, 1558
TasI AATT 4 cut(s) 349, 585, 616, 1140
TatI WGTACW 2 cut(s) 40, 1421
Tru1I TTAA 4 cut(s) 1187, 1287, 1503, 1570
Tru9I TTAA 4 cut(s) 1187, 1287, 1503, 1570
TscAI CASTG 3 cut(s) 319, 645, 1348
TseFI GTSAC 4 cut(s) 46, 427, 757, 1506
TseI GCWGC 2 cut(s) 647, 809
Tsp45I GTSAC 4 cut(s) 46, 427, 757, 1506
TspDTI ATGAA 6 cut(s) 153, 391, 754, 813, 1065, 1452
TspGWI ACGGA 2 cut(s) 186, 1233
TspRI CASTG 3 cut(s) 319, 645, 1348
Vha464I CTTAAG 1 cut(s) 1186
XceI RCATGY 3 cut(s) 57, 843, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XmiI GTMKAC 1 cut(s) 897
ZrmI AGTACT 1 cut(s) 1423
Zsp2I ATGCAT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.