pycom01g13450

Uncharacterized ACR, COG1399

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014617
Physical Location & Seq
Reverse (-)
13888043 .. 13889672
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g13450.1

Sequence Viewer

Length: 963 bp
ATGTCATTTGTAGTCACCTCATCCTCCATTGCCACCTCCTTGTACTTTAACCGCCATAGTGTTTCTAAATTAAAATCCTGTGAGAATACTACGAAATATTCATGTGTTCCGTTTGTTCATTGCAAAAGCCCATTTTGCATTACAGAAAATATGCGTACAACTTCTACAAGAAACCCAAATGGCATTTTGAGGTCTACTGTAATGAGCTGCACAAAGCCTGATTATCAGTCTTTTGCTGGAGAAGACGCGGTTTTTCTTGACTTGGGGGATAATGAAGACACAGAGTCGCCCTGGGAAGGGGCAGTAATTTACAAGAGAAACGCTTCGATATCACATGTTGAGTATTGCACAACGTTGGAGAGGCTTGGATTGGAGAACCTTTCAACAGAGGTCTCGAAATCTAGGGCTTCGGTTATGGGATTACGGGTCACCAAAGCTGTGAAAGATTATCCACAGGGCACACCAGTTCAGATCTCCATTGACATAACTAGGAAGAAGCAAAAGCTGAGGCTTGATGGGATCATTAAAACTGTTATCACGCTGACTTGTAGCAGGTGTGGAGAGCCAGCGGCTGAGTGCATTTTCTCCAACTTCGCCCTTCTCCTAACTGATGAACCCATTGAAGAACCTGATATCATCAATCTGGGAGTGATGTATGGGGACAATTCCAAAACTTATACCGAATTTGGTGACCAAGAGGAGGAAGATGATGAAGCCGCAATTGACTTGGAGGATCAGCTGTATTTTCGCCCCGATGAAAAAGAAATAGACATTTCAAAGCACATAAGAGACGCCGTGCATCTGGAGATTACAATCGCGGCAGTATGTGATCCGAGTTGCAAGGGTTTGTGTCTCGGTTGTGGTAGGAATTTGAACACTAGCAGTTGTACTTGCAGTAAAAAGGAGGTAAAGAAGGGTCTAGGACCTCTTGGAAACTTAAAGAAGCAAATGCAGCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF177

Protein Analysis

321

Amino Acids

35.51

Weight (kDa)

5.44

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YceD PF02620 223 - 314 1.9e-16 Large ribosomal RNA subunit accumulation protein YceD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014617)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19810 AT3G19810
fragaria_vesca FvH4_7g19250
malus_domestica MD01G1107600.v1.1
prunus_persica Prupe.2G212300_v2.0.a1
pyrus_communis pycom01g13450
rosa_chinensis RchiOBHm_Chr1g0363071
rosa_laevigata RLG00000027596
rosa_multiflora Rmu_sc0014047.1_g000001
rosa_roxburghii Rroxscaffold_4G00293490
rosa_rugosa Rorug01G0304300 Rorug01G0304400
rosa_samantha Rh1AG313400 Rh1BG276500 Rh1CG293300 Rh1DG307200
rosa_wichuraiana Rw1G027760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 543
Acc36I ACCTGC 1 cut(s) 543
AccI GTMKAC 1 cut(s) 194
AccII CGCG 2 cut(s) 248, 818
AciI CCGC 5 cut(s) 52, 248, 569, 717, 818
AclI AACGTT 1 cut(s) 353
AclWI GGATC 3 cut(s) 527, 741, 824
AcsI RAATTY 2 cut(s) 683, 868
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 3 cut(s) 44, 157, 889
AfiI CCNNNNNNNGG 3 cut(s) 296, 297, 700
AflIII ACRYGT 1 cut(s) 334
AgsI TTSAA 4 cut(s) 384, 623, 777, 874
AhdI GACNNNNNGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 290
AluBI AGCT 4 cut(s) 207, 437, 505, 739
AluI AGCT 4 cut(s) 207, 437, 505, 739
Alw26I GTCTC 3 cut(s) 397, 783, 857
AlwI GGATC 3 cut(s) 527, 741, 824
AlwNI CAGNNNCTG 1 cut(s) 572
ApeKI GCWGC 2 cut(s) 207, 952
ApoI RAATTY 2 cut(s) 683, 868
Asp700I GAANNNNTTC 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 923
AsuHPI GGTGA 3 cut(s) 7, 421, 701
AvaII GGWCC 1 cut(s) 923
BaeGI GKGCMC 1 cut(s) 461
BbsI GAAGAC 2 cut(s) 249, 282
BbvCI CCTCAGC 1 cut(s) 506
BbvI GCAGC 1 cut(s) 194
BccI CCATC 1 cut(s) 509
BceAI ACGGC 1 cut(s) 779
BciT130I CCWGG 1 cut(s) 292
BcoDI GTCTC 3 cut(s) 397, 783, 857
BfaI CTAG 4 cut(s) 402, 489, 879, 920
BfuAI ACCTGC 1 cut(s) 543
BglII AGATCT 1 cut(s) 471
BisI GCNGC 5 cut(s) 208, 570, 717, 819, 953
BlsI GCNGC 5 cut(s) 209, 571, 718, 820, 954
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 923
BmeRI GACNNNNNGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 923
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 808
BpiI GAAGAC 2 cut(s) 249, 282
BpmI CTGGAG 2 cut(s) 258, 824
Bpu10I CCTNAGC 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 812
BsaHI GRCGYC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 290, 291
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 297, 700
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 2 cut(s) 27, 118
Bse8I GATNNNNATC 1 cut(s) 812
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 2 cut(s) 290, 291
BseGI GGATG 1 cut(s) 20
BseJI GATNNNNATC 1 cut(s) 812
BseLI CCNNNNNNNGG 3 cut(s) 296, 297, 700
BseMI GCAATG 2 cut(s) 27, 118
BseMII CTCAG 2 cut(s) 497, 564
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 713
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 1 cut(s) 194
BsgI GTGCAG 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 248, 818
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 3 cut(s) 296, 297, 700
BsmAI GTCTC 3 cut(s) 397, 783, 857
BsmBI CGTCTC 1 cut(s) 783
BsmFI GGGAC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 4 cut(s) 471, 519, 733, 829
BspACI CCGC 5 cut(s) 52, 248, 569, 717, 818
BspCNI CTCAG 2 cut(s) 498, 565
BspFNI CGCG 2 cut(s) 248, 818
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 3 cut(s) 527, 741, 824
BspTNI GGTCTC 1 cut(s) 397
BsrDI GCAATG 2 cut(s) 27, 118
BsrI ACTGG 1 cut(s) 464
BssECI CCNNGG 2 cut(s) 290, 291
BssMI GATC 4 cut(s) 471, 519, 733, 829
BssNI GRCGYC 1 cut(s) 792
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 2 cut(s) 199, 532
BstACI GRCGYC 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 2 cut(s) 506, 573
BstEII GGTNACC 2 cut(s) 427, 689
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 2 cut(s) 248, 818
BstKTI GATC 4 cut(s) 474, 522, 736, 832
BstMAI GTCTC 3 cut(s) 397, 783, 857
BstMBI GATC 4 cut(s) 471, 519, 733, 829
BstMWI GCNNNNNNNGC 2 cut(s) 135, 952
BstNI CCWGG 1 cut(s) 292
BstNSI RCATGY 1 cut(s) 338
BstPI GGTNACC 2 cut(s) 427, 689
BstSCI CCNGG 1 cut(s) 290
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 2 cut(s) 248, 818
BstV1I GCAGC 1 cut(s) 194
BstV2I GAAGAC 2 cut(s) 249, 282
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
BtsCI GGATG 1 cut(s) 20
BveI ACCTGC 1 cut(s) 543
Cac8I GCNNGC 1 cut(s) 567
CaiI CAGNNNCTG 1 cut(s) 572
Cfr13I GGNCC 1 cut(s) 923
CseI GACGC 2 cut(s) 254, 800
Csp6I GTAC 3 cut(s) 43, 156, 888
CviAII CATG 2 cut(s) 102, 335
CviQI GTAC 3 cut(s) 43, 156, 888
DdeI CTNAG 2 cut(s) 506, 573
DpnI GATC 4 cut(s) 473, 521, 735, 831
DpnII GATC 4 cut(s) 471, 519, 733, 829
DriI GACNNNNNGTC 1 cut(s) 283
Eam1105I GACNNNNNGTC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 397
Eco32I GATATC 2 cut(s) 330, 634
Eco47I GGWCC 1 cut(s) 923
Eco91I GGTNACC 2 cut(s) 427, 689
EcoO109I RGGNCCY 1 cut(s) 923
EcoO65I GGTNACC 2 cut(s) 427, 689
EcoRII CCWGG 1 cut(s) 290
EcoRV GATATC 2 cut(s) 330, 634
Esp3I CGTCTC 1 cut(s) 783
FaeI CATG 2 cut(s) 105, 338
FaqI GGGAC 1 cut(s) 674
FatI CATG 2 cut(s) 101, 334
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 5 cut(s) 208, 570, 717, 819, 953
FokI GGATG 1 cut(s) 7
Fsp4HI GCNGC 5 cut(s) 208, 570, 717, 819, 953
FspBI CTAG 4 cut(s) 402, 489, 879, 920
GluI GCNGC 5 cut(s) 208, 570, 717, 819, 953
GsuI CTGGAG 2 cut(s) 258, 824
HgaI GACGC 2 cut(s) 254, 800
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 2 cut(s) 105, 338
HinfI GANTC 1 cut(s) 284
HphI GGTGA 3 cut(s) 7, 421, 701
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 2 cut(s) 471, 834
Hpy188III TCNNGA 3 cut(s) 257, 394, 803
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 3 cut(s) 290, 608, 907
HpyCH4III ACNGT 2 cut(s) 199, 532
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 9 cut(s) 123, 138, 210, 348, 579, 799, 840, 894, 952
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 952
HpyF3I CTNAG 2 cut(s) 506, 573
HpySE526I ACGT 1 cut(s) 353
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 2 cut(s) 105, 338
Kzo9I GATC 4 cut(s) 471, 519, 733, 829
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 1 cut(s) 808
MaeI CTAG 4 cut(s) 402, 489, 879, 920
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 3 cut(s) 13, 427, 689
MalI GATC 4 cut(s) 473, 521, 735, 831
MboI GATC 4 cut(s) 471, 519, 733, 829
MboII GAAGA 5 cut(s) 254, 287, 505, 635, 716
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 6 cut(s) 68, 306, 664, 683, 720, 868
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 336, 612
MroXI GAANNNNTTC 1 cut(s) 322
MseI TTAA 4 cut(s) 48, 71, 525, 938
MspA1I CMGCKG 2 cut(s) 569, 739
MspR9I CCNGG 1 cut(s) 292
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 1 cut(s) 292
MvnI CGCG 2 cut(s) 248, 818
MwoI GCNNNNNNNGC 2 cut(s) 135, 952
NdeII GATC 4 cut(s) 471, 519, 733, 829
NlaIII CATG 2 cut(s) 105, 338
NmuCI GTSAC 3 cut(s) 13, 427, 689
NspI RCATGY 1 cut(s) 338
PaqCI CACCTGC 1 cut(s) 543
PasI CCCWGGG 1 cut(s) 291
PciI ACATGT 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 322
PkrI GCNGC 5 cut(s) 209, 571, 718, 820, 954
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PpuMI RGGWCCY 1 cut(s) 923
PscI ACATGT 1 cut(s) 334
Psp1406I AACGTT 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 290
PspEI GGTNACC 2 cut(s) 427, 689
PspGI CCWGG 1 cut(s) 290
PspPI GGNCC 1 cut(s) 923
PspPPI RGGWCCY 1 cut(s) 923
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 1 cut(s) 471
PvuII CAGCTG 1 cut(s) 739
RsaI GTAC 3 cut(s) 44, 157, 889
RsaNI GTAC 3 cut(s) 43, 156, 888
SaqAI TTAA 4 cut(s) 48, 71, 525, 938
SatI GCNGC 5 cut(s) 208, 570, 717, 819, 953
Sau3AI GATC 4 cut(s) 471, 519, 733, 829
Sau96I GGNCC 1 cut(s) 923
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 1 cut(s) 808
SinI GGWCC 1 cut(s) 923
Sse9I AATT 6 cut(s) 68, 306, 664, 683, 720, 868
SsiI CCGC 5 cut(s) 52, 248, 569, 717, 818
SspI AATATT 1 cut(s) 98
SspMI CTAG 4 cut(s) 402, 489, 879, 920
StyD4I CCNGG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 199, 532
TaiI ACGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 326, 395
TasI AATT 6 cut(s) 68, 306, 664, 683, 720, 868
TatI WGTACW 2 cut(s) 42, 887
TauI GCSGC 3 cut(s) 572, 719, 821
Tru1I TTAA 4 cut(s) 48, 71, 525, 938
Tru9I TTAA 4 cut(s) 48, 71, 525, 938
TseFI GTSAC 3 cut(s) 13, 427, 689
TseI GCWGC 2 cut(s) 207, 952
Tsp45I GTSAC 3 cut(s) 13, 427, 689
TspDTI ATGAA 6 cut(s) 90, 107, 288, 627, 726, 771
TspGWI ACGGA 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 923
XapI RAATTY 2 cut(s) 683, 868
XceI RCATGY 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 4 cut(s) 402, 489, 879, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.